Matching Items (10)
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- All Subjects: Biosensors
- All Subjects: Security
- Genre: Doctoral Dissertation
- Creators: Goryll, Michael
- Creators: Zhang, Junshan
- Member of: ASU Electronic Theses and Dissertations
Description
Over the past fifty years, the development of sensors for biological applications has increased dramatically. This rapid growth can be attributed in part to the reduction in feature size, which the electronics industry has pioneered over the same period. The decrease in feature size has led to the production of microscale sensors that are used for sensing applications, ranging from whole-body monitoring down to molecular sensing. Unfortunately, sensors are often developed without regard to how they will be integrated into biological systems. The complexities of integration are underappreciated. Integration involves more than simply making electrical connections. Interfacing microscale sensors with biological environments requires numerous considerations with respect to the creation of compatible packaging, the management of biological reagents, and the act of combining technologies with different dimensions and material properties. Recent advances in microfluidics, especially the proliferation of soft lithography manufacturing methods, have established the groundwork for creating systems that may solve many of the problems inherent to sensor-fluidic interaction. The adaptation of microelectronics manufacturing methods, such as Complementary Metal-Oxide-Semiconductor (CMOS) and Microelectromechanical Systems (MEMS) processes, allows the creation of a complete biological sensing system with integrated sensors and readout circuits. Combining these technologies is an obstacle to forming complete sensor systems. This dissertation presents new approaches for the design, fabrication, and integration of microscale sensors and microelectronics with microfluidics. The work addresses specific challenges, such as combining commercial manufacturing processes into biological systems and developing microscale sensors in these processes. This work is exemplified through a feedback-controlled microfluidic pH system to demonstrate the integration capabilities of microscale sensors for autonomous microenvironment control.
ContributorsWelch, David (Author) / Blain Christen, Jennifer (Thesis advisor) / Muthuswamy, Jitendran (Committee member) / Frakes, David (Committee member) / LaBelle, Jeffrey (Committee member) / Goryll, Michael (Committee member) / Arizona State University (Publisher)
Created2012
Description
Adversarial threats of deep learning are increasingly becoming a concern due to the ubiquitous deployment of deep neural networks(DNNs) in many security-sensitive domains. Among the existing threats, adversarial weight perturbation is an emerging class of threats that attempts to perturb the weight parameters of DNNs to breach security and privacy.In this thesis, the first weight perturbation attack introduced is called Bit-Flip Attack (BFA), which can maliciously flip a small number of bits within a computer’s main memory system storing the DNN weight parameter to achieve malicious objectives. Our developed algorithm can achieve three specific attack objectives: I) Un-targeted accuracy degradation attack, ii) Targeted attack, & iii) Trojan attack. Moreover, BFA utilizes the rowhammer technique to demonstrate the bit-flip attack in an actual computer prototype. While the bit-flip attack is conducted in a white-box setting, the subsequent contribution of this thesis is to develop another novel weight perturbation attack in a black-box setting. Consequently, this thesis discusses a new study of DNN model vulnerabilities in a multi-tenant Field Programmable Gate Array (FPGA) cloud under a strict black-box framework. This newly developed attack framework injects faults in the malicious tenant by duplicating specific DNN weight packages during data transmission between off-chip memory and on-chip buffer of a victim FPGA. The proposed attack is also experimentally validated in a multi-tenant cloud FPGA prototype. In the final part, the focus shifts toward deep learning model privacy, popularly known as model extraction, that can steal partial DNN weight parameters remotely with the aid of a memory side-channel attack. In addition, a novel training algorithm is designed to utilize the partially leaked DNN weight bit information, making the model extraction attack more effective. The algorithm effectively leverages the partial leaked bit information and generates a substitute prototype of the victim model with almost identical performance to the victim.
ContributorsRakin, Adnan Siraj (Author) / Fan, Deliang (Thesis advisor) / Chakrabarti, Chaitali (Committee member) / Seo, Jae-Sun (Committee member) / Cao, Yu (Committee member) / Arizona State University (Publisher)
Created2022
Description
Mobile devices have penetrated into every aspect of modern world. For one thing, they are becoming ubiquitous in daily life. For the other thing, they are storing more and more data, including sensitive data. Therefore, security and privacy of mobile devices are indispensable. This dissertation consists of five parts: two authentication schemes, two attacks, and one countermeasure related to security and privacy of mobile devices.
Specifically, in Chapter 1, I give an overview the challenges and existing solutions in these areas. In Chapter 2, a novel authentication scheme is presented, which is based on a user’s tapping or sliding on the touchscreen of a mobile device. In Chapter 3, I focus on mobile app fingerprinting and propose a method based on analyzing the power profiles of targeted mobile devices. In Chapter 4, I mainly explore a novel liveness detection method for face authentication on mobile devices. In Chapter 5, I investigate a novel keystroke inference attack on mobile devices based on user eye movements. In Chapter 6, a novel authentication scheme is proposed, based on detecting a user’s finger gesture through acoustic sensing. In Chapter 7, I discuss the future work.
Specifically, in Chapter 1, I give an overview the challenges and existing solutions in these areas. In Chapter 2, a novel authentication scheme is presented, which is based on a user’s tapping or sliding on the touchscreen of a mobile device. In Chapter 3, I focus on mobile app fingerprinting and propose a method based on analyzing the power profiles of targeted mobile devices. In Chapter 4, I mainly explore a novel liveness detection method for face authentication on mobile devices. In Chapter 5, I investigate a novel keystroke inference attack on mobile devices based on user eye movements. In Chapter 6, a novel authentication scheme is proposed, based on detecting a user’s finger gesture through acoustic sensing. In Chapter 7, I discuss the future work.
ContributorsChen, Yimin (Author) / Zhang, Yanchao (Thesis advisor) / Zhang, Junshan (Committee member) / Reisslein, Martin (Committee member) / Ying, Lei (Committee member) / Arizona State University (Publisher)
Created2018
Description
Microblogging services such as Twitter, Sina Weibo, and Tumblr have been emerging and deeply embedded into people's daily lives. Used by hundreds of millions of users to connect the people worldwide and share and access information in real-time, the microblogging service has also became the target of malicious attackers due to its massive user engagement and structural openness. Although existed, little is still known in the community about new types of vulnerabilities in current microblogging services which could be leveraged by the intelligence-evolving attackers, and more importantly, the corresponding defenses that could prevent both the users and the microblogging service providers from being attacked. This dissertation aims to uncover a number of challenging security and privacy issues in microblogging services and also propose corresponding defenses.
This dissertation makes fivefold contributions. The first part presents the social botnet, a group of collaborative social bots under the control of a single botmaster, demonstrate the effectiveness and advantages of exploiting a social botnet for spam distribution and digital-influence manipulation, and propose the corresponding countermeasures and evaluate their effectiveness. Inspired by Pagerank, the second part describes TrueTop, the first sybil-resilient system to find the top-K influential users in microblogging services with very accurate results and strong resilience to sybil attacks. TrueTop has been implemented to handle millions of nodes and 100 times more edges on commodity computers. The third and fourth part demonstrate that microblogging systems' structural openness and users' carelessness could disclose the later's sensitive information such as home city and age. LocInfer, a novel and lightweight system, is presented to uncover the majority of the users in any metropolitan area; the dissertation also proposes MAIF, a novel machine learning framework that leverages public content and interaction information in microblogging services to infer users' hidden ages. Finally, the dissertation proposes the first privacy-preserving social media publishing framework to let the microblogging service providers publish their data to any third-party without disclosing users' privacy and meanwhile meeting the data's commercial utilities. This dissertation sheds the light on the state-of-the-art security and privacy issues in the microblogging services.
This dissertation makes fivefold contributions. The first part presents the social botnet, a group of collaborative social bots under the control of a single botmaster, demonstrate the effectiveness and advantages of exploiting a social botnet for spam distribution and digital-influence manipulation, and propose the corresponding countermeasures and evaluate their effectiveness. Inspired by Pagerank, the second part describes TrueTop, the first sybil-resilient system to find the top-K influential users in microblogging services with very accurate results and strong resilience to sybil attacks. TrueTop has been implemented to handle millions of nodes and 100 times more edges on commodity computers. The third and fourth part demonstrate that microblogging systems' structural openness and users' carelessness could disclose the later's sensitive information such as home city and age. LocInfer, a novel and lightweight system, is presented to uncover the majority of the users in any metropolitan area; the dissertation also proposes MAIF, a novel machine learning framework that leverages public content and interaction information in microblogging services to infer users' hidden ages. Finally, the dissertation proposes the first privacy-preserving social media publishing framework to let the microblogging service providers publish their data to any third-party without disclosing users' privacy and meanwhile meeting the data's commercial utilities. This dissertation sheds the light on the state-of-the-art security and privacy issues in the microblogging services.
ContributorsZhang, Jinxue (Author) / Zhang, Yanchao (Thesis advisor) / Zhang, Junshan (Committee member) / Ying, Lei (Committee member) / Ahn, Gail-Joon (Committee member) / Arizona State University (Publisher)
Created2016
Description
Biosensors aiming at detection of target analytes, such as proteins, microbes, virus, and toxins, are widely needed for various applications including detection of chemical and biological warfare (CBW) agents, biomedicine, environmental monitoring, and drug screening. Surface Plasmon Resonance (SPR), as a surface-sensitive analytical tool, can very sensitively respond to minute changes of refractive index occurring adjacent to a metal film, offering detection limits up to a few ppt (pg/mL). Through SPR, the process of protein adsorption may be monitored in real-time, and transduced into an SPR angle shift. This unique technique bypasses the time-consuming, labor-intensive labeling processes, such as radioisotope and fluorescence labeling. More importantly, the method avoids the modification of the biomarker’s characteristics and behaviors by labeling that often occurs in traditional biosensors. While many transducers, including SPR, offer high sensitivity, selectivity is determined by the bio-receptors. In traditional biosensors, the selectivity is provided by bio-receptors possessing highly specific binding affinity to capture target analytes, yet their use in biosensors are often limited by their relatively-weak binding affinity with analyte, non-specific adsorption, need for optimization conditions, low reproducibility, and difficulties integrating onto the surface of transducers. In order to circumvent the use of bio-receptors, the competitive adsorption of proteins, termed the Vroman effect, is utilized in this work. The Vroman effect was first reported by Vroman and Adams in 1969. The competitive adsorption targeted here occurs among different proteins competing to adsorb to a surface, when more than one type of protein is present. When lower-affinity proteins are adsorbed on the surface first, they can be displaced by higher-affinity proteins arriving at the surface at a later point in time. Moreover, only low-affinity proteins can be displaced by high-affinity proteins, typically possessing higher molecular weight, yet the reverse sequence does not occur. The SPR biosensor based on competitive adsorption is successfully demonstrated to detect fibrinogen and thyroglobulin (Tg) in undiluted human serum and copper ions in drinking water through the denatured albumin.
ContributorsWang, Ran (Author) / Chae, Junseok (Thesis advisor) / Bakkaloglu, Bertan (Committee member) / Tsow, Tsing (Committee member) / Goryll, Michael (Committee member) / Arizona State University (Publisher)
Created2015
Description
This work explores how flexible electronics and display technology can be applied to develop new biomedical devices for medical, biological, and life science applications. It demonstrates how new biomedical devices can be manufactured by only modifying or personalizing the upper layers of a conventional thin film transistor (TFT) display process. This personalization was applied first to develop and demonstrate the world's largest flexible digital x-ray detector for medical and industrial imaging, and the world's first flexible ISFET pH biosensor using TFT technology. These new, flexible, digital x-ray detectors are more durable than conventional glass substrate x-ray detectors, and also can conform to the surface of the object being imaged. The new flexible ISFET pH biosensors are >10X less expensive to manufacture than comparable CMOS-based ISFETs and provide a sensing area that is orders of magnitude larger than CMOS-based ISFETs. This allows for easier integration with area intensive chemical and biological recognition material as well as allow for a larger number of unique recognition sites for low cost multiple disease and pathogen detection.
The flexible x-ray detector technology was then extended to demonstrate the viability of a new technique to seamlessly combine multiple smaller flexible x-ray detectors into a single very large, ultimately human sized, composite x-ray detector for new medical imaging applications such as single-exposure, low-dose, full-body digital radiography. Also explored, is a new approach to increase the sensitivity of digital x-ray detectors by selectively disabling rows in the active matrix array that are not part of the imaged region. It was then shown how high-resolution, flexible, organic light-emitting diode display (OLED) technology can be used to selectively stimulate and/or silence small groups of neurons on the cortical surface or within the deep brain as a potential new tool to diagnose and treat, as well as understand, neurological diseases and conditions. This work also explored the viability of a new miniaturized high sensitivity fluorescence measurement-based lab-on-a-chip optical biosensor using OLED display and a-Si:H PiN photodiode active matrix array technology for point-of-care diagnosis of multiple disease or pathogen biomarkers in a low cost disposable configuration.
The flexible x-ray detector technology was then extended to demonstrate the viability of a new technique to seamlessly combine multiple smaller flexible x-ray detectors into a single very large, ultimately human sized, composite x-ray detector for new medical imaging applications such as single-exposure, low-dose, full-body digital radiography. Also explored, is a new approach to increase the sensitivity of digital x-ray detectors by selectively disabling rows in the active matrix array that are not part of the imaged region. It was then shown how high-resolution, flexible, organic light-emitting diode display (OLED) technology can be used to selectively stimulate and/or silence small groups of neurons on the cortical surface or within the deep brain as a potential new tool to diagnose and treat, as well as understand, neurological diseases and conditions. This work also explored the viability of a new miniaturized high sensitivity fluorescence measurement-based lab-on-a-chip optical biosensor using OLED display and a-Si:H PiN photodiode active matrix array technology for point-of-care diagnosis of multiple disease or pathogen biomarkers in a low cost disposable configuration.
ContributorsSmith, Joseph T. (Author) / Allee, David (Thesis advisor) / Goryll, Michael (Committee member) / Kozicki, Michael (Committee member) / Blain Christen, Jennifer (Committee member) / Couture, Aaron (Committee member) / Arizona State University (Publisher)
Created2014
Description
A principal goal of this dissertation is to study wireless network design and optimization with the focus on two perspectives: 1) socially-aware mobile networking and computing; 2) security and privacy in wireless networking. Under this common theme, this dissertation can be broadly organized into three parts.
The first part studies socially-aware mobile networking and computing. First, it studies random access control and power control under a social group utility maximization (SGUM) framework. The socially-aware Nash equilibria (SNEs) are derived and analyzed. Then, it studies mobile crowdsensing under an incentive mechanism that exploits social trust assisted reciprocity (STAR). The efficacy of the STAR mechanism is thoroughly investigated. Next, it studies mobile users' data usage behaviors under the impact of social services and the wireless operator's pricing. Based on a two-stage Stackelberg game formulation, the user demand equilibrium (UDE) is analyzed in Stage II and the optimal pricing strategy is developed in Stage I. Last, it studies opportunistic cooperative networking under an optimal stopping framework with two-level decision-making. For both cases with or without dedicated relays, the optimal relaying strategies are derived and analyzed.
The second part studies radar sensor network coverage for physical security. First, it studies placement of bistatic radar (BR) sensor networks for barrier coverage. The optimality of line-based placement is analyzed, and the optimal placement of BRs on a line segment is characterized. Then, it studies the coverage of radar sensor networks that exploits the Doppler effect. Based on a Doppler coverage model, an efficient method is devised to characterize Doppler-covered regions and an algorithm is developed to find the minimum radar density required for Doppler coverage.
The third part studies cyber security and privacy in socially-aware networking and computing. First, it studies random access control, cooperative jamming, and spectrum access under an extended SGUM framework that incorporates negative social ties. The SNEs are derived and analyzed. Then, it studies pseudonym change for personalized location privacy under the SGUM framework. The SNEs are analyzed and an efficient algorithm is developed to find an SNE with desirable properties.
The first part studies socially-aware mobile networking and computing. First, it studies random access control and power control under a social group utility maximization (SGUM) framework. The socially-aware Nash equilibria (SNEs) are derived and analyzed. Then, it studies mobile crowdsensing under an incentive mechanism that exploits social trust assisted reciprocity (STAR). The efficacy of the STAR mechanism is thoroughly investigated. Next, it studies mobile users' data usage behaviors under the impact of social services and the wireless operator's pricing. Based on a two-stage Stackelberg game formulation, the user demand equilibrium (UDE) is analyzed in Stage II and the optimal pricing strategy is developed in Stage I. Last, it studies opportunistic cooperative networking under an optimal stopping framework with two-level decision-making. For both cases with or without dedicated relays, the optimal relaying strategies are derived and analyzed.
The second part studies radar sensor network coverage for physical security. First, it studies placement of bistatic radar (BR) sensor networks for barrier coverage. The optimality of line-based placement is analyzed, and the optimal placement of BRs on a line segment is characterized. Then, it studies the coverage of radar sensor networks that exploits the Doppler effect. Based on a Doppler coverage model, an efficient method is devised to characterize Doppler-covered regions and an algorithm is developed to find the minimum radar density required for Doppler coverage.
The third part studies cyber security and privacy in socially-aware networking and computing. First, it studies random access control, cooperative jamming, and spectrum access under an extended SGUM framework that incorporates negative social ties. The SNEs are derived and analyzed. Then, it studies pseudonym change for personalized location privacy under the SGUM framework. The SNEs are analyzed and an efficient algorithm is developed to find an SNE with desirable properties.
ContributorsGong, Xiaowen (Author) / Zhang, Junshan (Thesis advisor) / Cochran, Douglas (Committee member) / Ying, Lei (Committee member) / Zhang, Yanchao (Committee member) / Arizona State University (Publisher)
Created2015
Description
Mobile devices are penetrating everyday life. According to a recent Cisco report [10], the number of mobile connected devices such as smartphones, tablets, laptops, eReaders, and Machine-to-Machine (M2M) modules will hit 11.6 billion by 2021, exceeding the world's projected population at that time (7.8 billion). The rapid development of mobile devices has brought a number of emerging security and privacy issues in mobile computing. This dissertation aims to address a number of challenging security and privacy issues in mobile computing.
This dissertation makes fivefold contributions. The first and second parts study the security and privacy issues in Device-to-Device communications. Specifically, the first part develops a novel scheme to enable a new way of trust relationship called spatiotemporal matching in a privacy-preserving and efficient fashion. To enhance the secure communication among mobile users, the second part proposes a game-theoretical framework to stimulate the cooperative shared secret key generation among mobile users. The third and fourth parts investigate the security and privacy issues in mobile crowdsourcing. In particular, the third part presents a secure and privacy-preserving mobile crowdsourcing system which strikes a good balance among object security, user privacy, and system efficiency. The fourth part demonstrates a differentially private distributed stream monitoring system via mobile crowdsourcing. Finally, the fifth part proposes VISIBLE, a novel video-assisted keystroke inference framework that allows an attacker to infer a tablet user's typed inputs on the touchscreen by recording and analyzing the video of the tablet backside during the user's input process. Besides, some potential countermeasures to this attack are also discussed. This dissertation sheds the light on the state-of-the-art security and privacy issues in mobile computing.
This dissertation makes fivefold contributions. The first and second parts study the security and privacy issues in Device-to-Device communications. Specifically, the first part develops a novel scheme to enable a new way of trust relationship called spatiotemporal matching in a privacy-preserving and efficient fashion. To enhance the secure communication among mobile users, the second part proposes a game-theoretical framework to stimulate the cooperative shared secret key generation among mobile users. The third and fourth parts investigate the security and privacy issues in mobile crowdsourcing. In particular, the third part presents a secure and privacy-preserving mobile crowdsourcing system which strikes a good balance among object security, user privacy, and system efficiency. The fourth part demonstrates a differentially private distributed stream monitoring system via mobile crowdsourcing. Finally, the fifth part proposes VISIBLE, a novel video-assisted keystroke inference framework that allows an attacker to infer a tablet user's typed inputs on the touchscreen by recording and analyzing the video of the tablet backside during the user's input process. Besides, some potential countermeasures to this attack are also discussed. This dissertation sheds the light on the state-of-the-art security and privacy issues in mobile computing.
ContributorsSun, Jingchao (Author) / Zhang, Yanchao (Thesis advisor) / Zhang, Junshan (Committee member) / Ying, Lei (Committee member) / Ahn, Gail-Joon (Committee member) / Arizona State University (Publisher)
Created2017
Description
Biogenic silica nanostructures, derived from diatoms, possess highly ordered porous hierarchical nanostructures and afford flexibility in design in large part due to the availability of a great variety of shapes, sizes, and symmetries. These advantages have been exploited for study of transport phenomena of ions and molecules towards the goal of developing ultrasensitive and selective filters and biosensors. Diatom frustules give researchers many inspiration and ideas for the design and production of novel nanostructured materials. In this doctoral research will focus on the following three aspects of biogenic silica: 1) Using diatom frustule as protein sensor. 2) Using diatom nanostructures as template to fabricate nano metal materials. 3) Using diatom nanostructures to fabricate hybrid platform.
Nanoscale confinement biogenetic silica template-based electrical biosensor assay offers the user the ability to detect and quantify the biomolecules. Diatoms have been demonstrated as part of a sensor. The sensor works on the principle of electrochemical impedance spectroscopy. When specific protein biomarkers from a test sample bind to corresponding antibodies conjugated to the surface of the gold surface at the base of each nanowell, a perturbation of electrical double layer occurs resulting in a change in the impedance.
Diatoms are also a new source of inspiration for the design and fabrication of nanostructured materials. Template-directed deposition within cylindrical nanopores of a porous membrane represents an attractive and reproducible approach for preparing metal nanopatterns or nanorods of a variety of aspect ratios. The nanopatterns fabricated from diatom have the potential of the metal-enhanced fluorescence to detect dye-conjugated molecules.
Another approach presents a platform integrating biogenic silica nanostructures with micromachined silicon substrates in a micro
ano hybrid device. In this study, one can take advantages of the unique properties of a marine diatom that exhibits nanopores on the order of 40 nm in diameter and a hierarchical structure. This device can be used to several applications, such as nano particles separation and detection. This platform is also a good substrate to study cell growth that one can observe the reaction of cell growing on the nanostructure of frustule.
Nanoscale confinement biogenetic silica template-based electrical biosensor assay offers the user the ability to detect and quantify the biomolecules. Diatoms have been demonstrated as part of a sensor. The sensor works on the principle of electrochemical impedance spectroscopy. When specific protein biomarkers from a test sample bind to corresponding antibodies conjugated to the surface of the gold surface at the base of each nanowell, a perturbation of electrical double layer occurs resulting in a change in the impedance.
Diatoms are also a new source of inspiration for the design and fabrication of nanostructured materials. Template-directed deposition within cylindrical nanopores of a porous membrane represents an attractive and reproducible approach for preparing metal nanopatterns or nanorods of a variety of aspect ratios. The nanopatterns fabricated from diatom have the potential of the metal-enhanced fluorescence to detect dye-conjugated molecules.
Another approach presents a platform integrating biogenic silica nanostructures with micromachined silicon substrates in a micro
ano hybrid device. In this study, one can take advantages of the unique properties of a marine diatom that exhibits nanopores on the order of 40 nm in diameter and a hierarchical structure. This device can be used to several applications, such as nano particles separation and detection. This platform is also a good substrate to study cell growth that one can observe the reaction of cell growing on the nanostructure of frustule.
ContributorsLin, Kai-Chun (Author) / Ramakrishna, B.L. (Thesis advisor) / Goryll, Michael (Thesis advisor) / Dey, Sandwip (Committee member) / Prasad, Shalini (Committee member) / Arizona State University (Publisher)
Created2014
Description
Engineered nanoporous substrates made using materials such as silicon nitride or silica have been demonstrated to work as particle counters or as hosts for nano-lipid bilayer membrane formation. These mechanically fabricated porous structures have thicknesses of several hundred nanometers up to several micrometers to ensure mechanical stability of the membrane. However, it is desirable to have a three-dimensional structure to ensure increased mechanical stability. In this study, circular silica shells used from Coscinodiscus wailesii, a species of diatoms (unicellular marine algae) were immobilized on a silicon chip with a micrometer-sized aperture using a UV curable polyurethane adhesive. The current conducted by a single nanopore of 40 nm diameter and 50 nm length, during the translocation of a 27 nm polystyrene sphere was simulated using COMSOL multiphysics and tested experimentally. The current conducted by a single 40 nm diameter nanopore of the diatom shell during the translocation of a 27 nm polystyrene sphere was simulated using COMSOL Multiphysics (28.36 pA) and was compared to the experimental measurement (28.69 pA) and Coulter Counting theory (29.95 pA).In addition, a mobility of 1.11 x 10-8 m2s-1V-1 for the 27 nm polystyrene spheres was used to convert the simulated current from spatial dependence to time dependence.
To achieve a sensing diameter of 1-2 nanometers, the diatom shells were used as substrates to perform ion-channel reconstitution experiments. The immobilized diatom shell was functionalized using silane chemistry and lipid bilayer membranes were formed. Functionalization of the diatom shell surface improves bilayer formation probability from 1 out of 10 to 10 out of 10 as monitored by impedance spectroscopy. Self-insertion of outer membrane protein OmpF of E.Coli into the lipid membranes could be confirmed using single channel recordings, indicating that nano-BLMs had formed which allow for fully functional porin activity. The results indicate that biogenic silica nanoporous substrates can be simulated using a simplified two dimensional geometry to predict the current when a nanoparticle translocates through a single aperture. With their tiered three-dimensional structure, diatom shells can be used in to form nano-lipid bilayer membranes and can be used in ion-channel reconstitution experiments similar to synthetic nanoporous membranes.
To achieve a sensing diameter of 1-2 nanometers, the diatom shells were used as substrates to perform ion-channel reconstitution experiments. The immobilized diatom shell was functionalized using silane chemistry and lipid bilayer membranes were formed. Functionalization of the diatom shell surface improves bilayer formation probability from 1 out of 10 to 10 out of 10 as monitored by impedance spectroscopy. Self-insertion of outer membrane protein OmpF of E.Coli into the lipid membranes could be confirmed using single channel recordings, indicating that nano-BLMs had formed which allow for fully functional porin activity. The results indicate that biogenic silica nanoporous substrates can be simulated using a simplified two dimensional geometry to predict the current when a nanoparticle translocates through a single aperture. With their tiered three-dimensional structure, diatom shells can be used in to form nano-lipid bilayer membranes and can be used in ion-channel reconstitution experiments similar to synthetic nanoporous membranes.
ContributorsRamakrishnan, Shankar (Author) / Goryll, Michael (Thesis advisor) / Blain Christen, Jennifer (Committee member) / Dey, Sandwip (Committee member) / Thornton, Trevor (Committee member) / Arizona State University (Publisher)
Created2015