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- All Subjects: Immunology
- Creators: School of Life Sciences
- Creators: Daliri, Ayoub
- Member of: Barrett, The Honors College Thesis/Creative Project Collection
- Resource Type: Text
PKR interaction mapped to a region within the dsRNA-binding domain of E3 and overlapped with sequences in the C-terminus of this domain that are necessary for binding to dsRNA. Point mutants of E3 were generated and screened for PKR inhibition and direct interaction. Analysis of these mutants demonstrates that dsRNA-binding but not PKR interaction plays a critical role in the broad host range of VACV. Nonetheless, full inhibition of PKR in cells in culture requires both dsRNA-binding and PKR interaction. Because E3 is highly conserved among orthopoxviruses, understanding the mechanisms that E3 uses to inhibit PKR can give insight into host range pathogenesis of dsRNA producing viruses.
The brain continuously monitors speech output to detect potential errors between its sensory prediction and its sensory production (Daliri et al., 2020). When the brain encounters an error, it generates a corrective motor response, usually in the opposite direction, to reduce the effect of the error. Previous studies have shown that the type of auditory error received may impact a participant’s corrective response. In this study, we examined whether participants respond differently to categorical or non-categorical errors. We applied two types of perturbation in real-time by shifting the first formant (F1) and second formant (F2) at three different magnitudes. The vowel /ɛ/ was shifted toward the vowel /æ/ in the categorical perturbation condition. In the non-categorical perturbation condition, the vowel /ɛ/ was shifted to a sound outside of the vowel quadrilateral (increasing both F1 and F2). Our results showed that participants responded to the categorical perturbation while they did not respond to the non-categorical perturbation. Additionally, we found that in the categorical perturbation condition, as the magnitude of the perturbation increased, the magnitude of the response increased. Overall, our results suggest that the brain may respond differently to categorical and non-categorical errors, and the brain is highly attuned to errors in speech.
Moraxella catarrhalis is a gram negative commensal bacteria that is a primary cause of otitis media in infants and severe exacerbations of COPD in adults. M. catarrhalis treatment has become increasingly difficult and expensive over the past half-century due to the emergence of beta-lactamase producing strains. There are currently no vaccines available to protect against infections. In this paper, we propose a transcriptomics-based approach for identifying potential vaccine targets. Additionally, a novel method was used to create bacterial vaccine polypeptides composed of sequence conserved peptides secreted through the outer membrane. Polypeptides were tested for immunogenicity and protective capacity in mice. We show that relative abundance of outer membrane proteins does not correlate with immunogenicity. We also show promising results for polypeptide protection in a mouse pulmonary clearance model.