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Hundreds of thousands of people die annually from malaria; a protozoan of the genus Plasmodium is responsible for this mortality. The Plasmodium parasite undergoes several life stages within the mosquito vector, the transition between which require passage across the lumen of the mosquito midgut. It has been observed that in about 15% of parasites that develop ookinetes in the mosquito abdomen, sporozoites never develop in the salivary glands, indicating that passage across the midgut lumen is a significant barrier in parasite development (Gamage-Mendis et al., 1993). We aim to investigate a possible correlation between passage through the midgut lumen and drug-resistance trends in Plasmodium falciparum parasites. This study contains a total of 1024 Anopheles mosquitoes: 187 Anopheles gambiae and 837 Anopheles funestus samples collected in high malaria transmission areas of Mozambique between March and June of 2016. Sanger sequencing will be used to determine the prevalence of known resistance alleles for anti-malarial drugs: chloroquine resistance transporter (pfcrt), multidrug resistance (pfmdr1) gene, dihydropteroate synthase (pfdhps) and dihydrofolate reductase (pfdhfr). We compare prevalence of resistance between abdomen and head/thorax in order to determine whether drug resistant parasites are disproportionately hindered during their passage through the midgut lumen. A statistically significant difference between resistance alleles in the two studied body sections supports the efficacy of new anti-malarial gene surveillance strategies in areas of high malaria transmission.
Mosquitoes are estimated to kill roughly 700,000 people each year through the transmission of vector-borne diseases. Vector control via insecticides is a widely used method in order to combat the spread of mosquito populations; however, this comes at a cost. Resistance to insecticides has the potential to increase vector-borne disease rates. Aedes aegypti is an invasive mosquito species in Arizona and is a known potential vector for a variety of infectious diseases including dengue, chikungunya, Zika, and yellow fever. In contrast to many other mosquito species Ae. aegypti mosquito eggs can undergo quiescence, an active state of dormancy, over long periods of time. Variation in quiescent periods correlates to climatic rainfall alterations and can ultimately influence hatching and mating between multiple generations. I have studied the effect of quiescence on larvicide (i.e., temephos) susceptibility using mosquito eggs collected from a susceptible lab strain and stored under optimal temperature and humidity conditions. After undergoing various quiescent periods (3, 7, 14, 28, 84, and 182 days), the experimental eggs as well as 7-day quiescent control eggs were hatched and reared to 3rd instar larvae. Temephos susceptibility was tested using the WHO bioassay procedure at lethal concentration (LC) 20, LC50, LC80, diagnostic dose (twice LC99), plus an untreated control. Each concentration dose was replicated four times with 20 larvae each. The 3-day experimental group was excluded from analysis because the mortality was significantly lower than the 7-day for both the experimental and control groups. The 3 day experimental eggs displayed decreased mortality which did not align with the hypothesis, as the quiescence period elongates under optimal conditions, susceptibility to insecticides decreases, and this could have likely resulted from unintentional selection for increased fitness and faster developing eggs because the larvae that developed to 3rd instar first were those used for larvicide testing. ANOVA testing demonstrates variability in the LC80 experimental group which suggests the need for further investigation into high dose temephos concentrations. For the experimental LC20 linear regression, there were significant differences in mortality. The results indicate mortality gradually decreases when the quiescence period elongates, therefore there are significant differences in insecticide susceptibility when quiescence is 182 days (or longer), compared to when quiescence is 7 days. Further investigation into field mosquito’s genetic diversity, insecticide resistance profile, and environmental conditions should be considered.
Activist burnout theory has produced minimal but meaningful literature and research that explores the dynamics of burnout culture, movement in-fighting, marginalized identities, and dimensions of burnout symptoms. Black feminist visionaries and writers such as Audre Lorde and bell hooks have developed theories of love, self-care and community as central to resistance that have informed my research approach. Thus, my study aims to investigate activist burnout from a perspective that marries popular activist burnout theory with these frameworks of self-care and community. I conducted a survey of Arizona State University student organizers and activists (N=34) to address the following research questions: What are the causes and symptoms of burnout for Arizona State University activists and organizers? How have self-care and community played a role in their work and countered burnout? Can working conceptions of self-care and community serve as resistance in ways that feel meaningful to activists? The survey was broken into three dimensions: “Demographics and Experience,” “Burnout,” and “Self-Care and Community.” The results reinforced prior findings on established toxic cultures and burnout symptoms but introduced complications to working theories, such as the connections between cycles of burnout and the cyclical nature of electoral politics along with the roles of chronic and mental illness. Respondents largely demonstrated conceptions of self-care and community as resistance but also demonstrated personal and professional barriers to putting these conceptions into practice.
Results indicate that there were social equity failures across all three sites with the private sites experiencing the most barriers for residents. The power-knowledge structure and perceptions of the residents shaped the institutions or staffing, services, policies, and amenities that either empowered the residents by helping them build a cohesive community; or it subjugated them by not offering space for community-building. In response, many residents' actions and beliefs were shaped by these institutions; however, in the face of resistance to management practices, they often exercised power through self-governing to achieve the satisfaction they desired. Recognizing that residents can exercise their own power, community resiliency to support aging in place may be achieved by supporting resident needs and drawing upon their expertise, assistance, and influential power to build stronger housing communities - an option with low costs but great gains. But in order to do so, the power-knowledge structure must be influenced to support this goal. This research describes the governance of public housing and the responses and relationships of both management and residents in these newly created public spaces. It then presents a model that can foster change in resident engagement and network building to support aging in place, and advance social and community resiliency, regardless of sector.
The oppressive legislative policies and polarizing media narratives of undocu/DACAmented Latinx im/migrants in the United States have created unfavorable campus climates, which have further marginalized those students in higher education who fit into this category. As a result of Donald Trump’s presidency and rescission of the Deferred Action for Childhood Arrivals (DACA) program that soon followed, undocu/DACAmented Latinx students are experiencing an increase in stress, anxiety, and fear to the point that they become silent, depressed, and feel the need to advocate more for their existence and worth on campus. My critical ethnographic case study investigates the everyday experiences of Mexican undocu/DACAmented students enrolled at a public university in Arizona – a state that borders Mexico – as they pursue their undergraduate degrees in the Trump era. This study is guided by critical race theory and LatCrit, sense of belonging, and resistance capital theoretical frameworks, and seeks to answer the following: (a) how race and racism shape their collegiate experiences, (b) where these collegians find belongingness to persist towards graduation while navigating an anti-im/migrant sociopolitical climate, and (c) how these students exercise agency via their activism efforts. The broader case study includes individual collaborative interviews, twelve months of participatory field observations, and a collection of documents. This study aims to expand the field of higher education’s understanding of how federal, state, and institutional policies and policymakers affect undocu/DACAmented students’ experiences in and persistence through college, highlight the agency exercised and assets these collegians bring with them to college, and offer research, policy, and practical recommendations for higher education and student affairs institutional agents.
