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- All Subjects: Civil Engineering
- All Subjects: Biology
- Creators: Arizona State University
- Creators: Zapata, Claudia
- Creators: Hjelmstad, Keith
Description
Studies have demonstrated that anthocyanins can function as antioxidants, reduce inflammation, and improve dyslipidemia. Tart cherries are anthocyanin-rich, making them particularly attractive as a functional food to improve cardiovascular disease (CVD) risk. There have been few published studies to date examining the impact of tart cherries on biomarkers of dyslipidemia and inflammation, particularly in overweight and obese individuals at high risk for these conditions. This study evaluated the effect of consuming 100% tart cherry juice daily on blood lipids including total cholesterol, low-density lipoprotein cholesterol (LDL-C), calculated very low density lipoprotein cholesterol (VLDL-C), triglycerides (TG), high density lipoprotein cholesterol (HDL-C), and the CVD risk ratios, as well as the inflammatory biomarkers interleukin 6 (IL-6), interleukin 10 (IL-10), tumor necrosis factor-alpha (TNF-alpha), C-reactive protein (CRP), monocyte chemotactic protein-1 (MCP-1), and erythrocyte sedimentation rate (ESR) following a 4-week period. Based on the high anthocyanin content of tart cherries, it was hypothesized that the lipid and inflammatory profiles would be significantly improved following the intervention. A total of 26 men and women completed this 4-week randomized, single-blind, placebo-controlled, crossover study. Participants were randomized to drink either 8 ounces of placebo beverage or tart cherry juice daily for 4 weeks. Following a 4-week washout period, the alternate beverage was consumed. Ultimately, this investigation demonstrated no statistically significant alterations in any of the lipid or inflammatory biomarkers when analyzed across time and between interventions (p > 0.05). As expected, glucose and insulin parameters remained stable over the duration of the study, as well as self-reported physical activity level, total calorie consumption, and macronutrient intake. However, trans-fat was reported to be significantly higher during the cherry arm of the study as compared to the placebo arm (p < 0.05), potentially confounding other results. Although the results of this study were equivocal, it is feasible that a higher dose, longer treatment duration, or more susceptible target population may be required to elicit significant effects. Consequently, further investigation is necessary to clarify this research.
ContributorsColes, Katie (Author) / Martin, Keith R. (Thesis advisor) / Traustadottir, Tinna (Committee member) / Vega-Lopez, Sonia (Committee member) / Arizona State University (Publisher)
Created2012
Description
This dissertation describes development of a procedure for obtaining high quality, optical grade sand coupons from frozen sand specimens of Ottawa 20/30 sand for image processing and analysis to quantify soil structure along with a methodology for quantifying the microstructure from the images. A technique for thawing and stabilizing frozen core samples was developed using optical grade Buehler® Epo-Tek® epoxy resin, a modified triaxial cell, a vacuum/reservoir chamber, a desiccator, and a moisture gauge. The uniform epoxy resin impregnation required proper drying of the soil specimen, application of appropriate confining pressure and vacuum levels, and epoxy mixing, de-airing and curing. The resulting stabilized sand specimen was sectioned into 10 mm thick coupons that were planed, ground, and polished with progressively finer diamond abrasive grit levels using the modified Allied HTP Inc. polishing method so that the soil structure could be accurately quantified using images obtained with the use of an optical microscopy technique. Illumination via Bright Field Microscopy was used to capture the images for subsequent image processing and sand microstructure analysis. The quality of resulting images and the validity of the subsequent image morphology analysis hinged largely on employment of a polishing and grinding technique that resulted in a flat, scratch free, reflective coupon surface characterized by minimal microstructure relief and good contrast between the sand particles and the surrounding epoxy resin. Subsequent image processing involved conversion of the color images first to gray scale images and then to binary images with the use of contrast and image adjustments, removal of noise and image artifacts, image filtering, and image segmentation. Mathematical morphology algorithms were used on the resulting binary images to further enhance image quality. The binary images were then used to calculate soil structure parameters that included particle roundness and sphericity, particle orientation variability represented by rose diagrams, statistics on the local void ratio variability as a function of the sample size, and the local void ratio distribution histograms using Oda's method and Voronoi tessellation method, including the skewness, kurtosis, and entropy of a gamma cumulative probability distribution fit to the local void ratio distribution.
ContributorsCzupak, Zbigniew David (Author) / Kavazanjian, Edward (Thesis advisor) / Zapata, Claudia (Committee member) / Houston, Sandra (Committee member) / Arizona State University (Publisher)
Created2011
Description
African Swine Fever (ASF), endemic in many African countries, is now spreading to other continents. Though ASF is capable of incurring serious economic losses in affected countries, no vaccine exists to provide immunity to animals. Disease control relies largely on rapid diagnosis and the implementation of movement restrictions and strict eradication programs. Developing a scalable, accurate and low cost diagnostic for ASF will be of great help for the current situation. CIM's 10K random peptide microarray is a new high-throughput platform that allows systematic investigations of immune responses associated with disease and shows promise as a diagnostic tool. In this study, this new technology was applied to characterize the immune responses of ASF virus (ASFV) infections and immunizations. Six sets of sera from ASFV antigen immunized pigs, 6 sera from infected pigs and 20 sera samples from unexposed pigs were tested and analyzed statistically. Results show that both ASFV antigen immunized pigs and ASFV viral infected pigs can be distinguished from unexposed pigs. Since it appears that immune responses to other viral infections are also distinguishable on this platform, it holds the potential of being useful in developing a new ASF diagnostic. The ability of this platform to identify specific ASFV antibody epitopes was also explored. A subtle motif was found to be shared among a set of peptides displaying the highest reactivity for an antigen specific antibody. However, this motif does not seem to match with any antibody epitopes predicted by a linear antibody epitope prediction.
ContributorsXiao, Liang (Author) / Sykes, Kathryn (Thesis advisor) / Zhao, Zhan-Gong (Committee member) / Stafford, Phillip (Committee member) / Arizona State University (Publisher)
Created2011
Description
The wood-framing trade has not sufficiently been investigated to understand the work task sequencing and coordination among crew members. A new mental framework for a performing crew was developed and tested through four case studies. This framework ensured similar team performance as the one provided by task micro-scheduling in planning software. It also allowed evaluation of the effect of individual coordination within the crew on the crew's productivity. Using design information, a list of micro-activities/tasks and their predecessors was automatically generated for each piece of lumber in the four wood frames. The task precedence was generated by applying elementary geometrical and technological reasoning to each frame. Then, the duration of each task was determined based on observations from videotaped activities. Primavera's (P6) resource leveling rules were used to calculate the sequencing of tasks and the minimum duration of the whole activity for various crew sizes. The results showed quick convergence towards the minimum production time and allowed to use information from Building Information Models (BIM) to automatically establish the optimal crew sizes for frames. Late Start (LS) leveling priority rule gave the shortest duration in every case. However, the logic of LS tasks rule is too complex to be conveyed to the framing crew. Therefore, the new mental framework of a well performing framer was developed and tested to ensure high coordination. This mental framework, based on five simple rules, can be easily taught to the crew and ensures a crew productivity congruent with the one provided by the LS logic. The case studies indicate that once the worst framer in the crew surpasses the limit of 11% deviation from applying the said five rules, every additional percent of deviation reduces the productivity of the whole crew by about 4%.
ContributorsMaghiar, Marcel M (Author) / Wiezel, Avi (Thesis advisor) / Mitropoulos, Panagiotis (Committee member) / Cooke, Nancy J. (Committee member) / Arizona State University (Publisher)
Created2011
Description
Once considered an abundant species in the eastern United States, local populations of red-shouldered hawks, Buteo lineatus, have declined due to habitat destruction. This destruction has created suitable habitat for red-tailed hawks, Buteo jamaicensis, and therefore increased competition between these two raptor species. Since suitable habitat is the main limiting factor for raptors, a computer model was created to simulate the effect of habitat loss in central Maryland and the impact of increased competition between the more aggressive red-tailed hawk. These simulations showed urban growth contributed to over a 30% increase in red-tailed hawk habitat as red-shouldered hawk habitat decreased 62.5-70.1% without competition and 71.8-76.3% with competition. However there was no significant difference seen between the rate of available habitat decline for current and predicted development growth.
ContributorsMurillo, Crystal (Author) / Whysong, Gary (Thesis advisor) / Alford, Eddie (Committee member) / Miller, William (Committee member) / Arizona State University (Publisher)
Created2011
Description
As a prelude to a study on the post-liquefaction properties and structure of soil, an investigation of ground freezing as an undisturbed sampling technique was conducted to investigate the ability of this sampling technique to preserve soil structure and properties. Freezing the ground is widely regarded as an appropriate technique to recover undisturbed samples of saturated cohesionless soil for laboratory testing, despite the fact that water increases in volume when frozen. The explanation generally given for the preservation of soil structure using the freezing technique was that, as long as the freezing front advanced uni-directionally, the expanding pore water is expelled ahead of the freezing front as the front advances. However, a literature review on the transition of water to ice shows that the volume of ice expands approximately nine percent after freezing, bringing into question the hypothesized mechanism and the ability of a frozen and then thawed specimen to retain the properties and structure of the soil in situ. Bench-top models were created by pluviation of sand. The soil in the model was then saturated and subsequently frozen. Freezing was accomplished using a pan filled with alcohol and dry ice placed on the surface of the sand layer to induce a unidirectional freezing front in the sample container. Coring was used to recover frozen samples from model containers. Recovered cores were then placed in a triaxial cell, thawed, and subjected to consolidated undrained loading. The stress-strain-strength behavior of the thawed cores was compared to the behavior of specimens created in a split mold by pluviation and then saturated and sheared without freezing and thawing. The laboratory testing provide insight to the impact of freezing and thawing on the properties of cohesionless soil.
ContributorsKatapa, Kanyembo (Author) / Kavazanjian, Edward (Thesis advisor) / Houston, Sandra (Committee member) / Zapata, Claudia (Committee member) / Arizona State University (Publisher)
Created2011
Description
Civil infrastructures are susceptible to damage under the events of natural or manmade disasters. Over the last two decades, the use of emerging engineering materials, such as the fiber-reinforced plastics (FRPs), in structural retrofitting have gained significant popularity. However, due to their inherent brittleness and lack of energy dissipation, undesirable failure modes of the FRP-retrofitted systems, such as sudden laminate fracture and debonding, have been frequently observed. In this light, a Carbon-fiber reinforced Hybrid-polymeric Matrix Composite (or CHMC) was developed to provide a superior, yet affordable, solution for infrastructure damage mitigation and protection. The microstructural and micromechanical characteristics of the CHMC was investigated using scanning electron microscopy (SEM) and nanoindentation technique. The mechanical performance, such as damping, was identified using free and forced vibration tests. A simplified analytical model based on micromechanics was developed to predict the laminate stiffness using the modulus profile tested by the nanoindentation. The prediction results were verified by the flexural modulus calculated from the vibration tests. The feasibility of using CHMC to retrofit damaged structural systems was investigated via a series of structural component level tests. The effectiveness of using CHMC versus conventional carbon-fiber reinforced epoxy (CF/ epoxy) to retrofit notch damaged steel beams were tested. The comparison of the test results indicated the superior deformation capacity of the CHMC retrofitted beams. The full field strain distributions near the critical notch tip region were experimentally determined by the digital imaging correlation (DIC), and the results matched well with the finite element analysis (FEA) results. In the second series of tests, the application of CHMC was expanded to retrofit the full-scale fatigue-damaged concrete-encased steel (or SRC) girders. Similar to the notched steel beam tests, the CHMC retrofitted SRC girders exhibited substantially better post-peak load ductility than that of CF/ epoxy retrofitted girder. Lastly, a quasi-static push over test on the CHMC retrofitted reinforced concrete shear wall further highlighted the CHMC's capability of enhancing the deformation and energy dissipating potential of the damaged civil infrastructure systems. Analytical and numerical models were developed to assist the retrofitting design using the newly developed CHMC material.
ContributorsZhou, Hongyu (Author) / Attard, Thomas L (Thesis advisor) / Fafitis, Apostolos (Thesis advisor) / Mignolet, Marc P (Committee member) / Ariaratnam, Samuel (Committee member) / Thomas, Benjamin (Committee member) / Blumsom, Jim (Committee member) / Arizona State University (Publisher)
Created2013
Description
Functional magnetic resonance imaging (fMRI) has been widely used to measure the retinotopic organization of early visual cortex in the human brain. Previous studies have identified multiple visual field maps (VFMs) based on statistical analysis of fMRI signals, but the resulting geometry has not been fully characterized with mathematical models. This thesis explores using concepts from computational conformal geometry to create a custom software framework for examining and generating quantitative mathematical models for characterizing the geometry of early visual areas in the human brain. The software framework includes a graphical user interface built on top of a selected core conformal flattening algorithm and various software tools compiled specifically for processing and examining retinotopic data. Three conformal flattening algorithms were implemented and evaluated for speed and how well they preserve the conformal metric. All three algorithms performed well in preserving the conformal metric but the speed and stability of the algorithms varied. The software framework performed correctly on actual retinotopic data collected using the standard travelling-wave experiment. Preliminary analysis of the Beltrami coefficient for the early data set shows that selected regions of V1 that contain reasonably smooth eccentricity and polar angle gradients do show significant local conformality, warranting further investigation of this approach for analysis of early and higher visual cortex.
ContributorsTa, Duyan (Author) / Wang, Yalin (Thesis advisor) / Maciejewski, Ross (Committee member) / Wonka, Peter (Committee member) / Arizona State University (Publisher)
Created2013
Description
Pathogenic Gram-negative bacteria employ a variety of molecular mechanisms to combat host defenses. Two-component regulatory systems (TCR systems) are the most ubiquitous signal transduction systems which regulate many genes required for virulence and survival of bacteria. In this study, I analyzed different TCR systems in two clinically-relevant Gram-negative bacteria, i.e., oral pathogen Porphyromonas gingivalis and enterobacterial Escherichia coli. P. gingivalis is a major causative agent of periodontal disease as well as systemic illnesses, like cardiovascular disease. A microarray study found that the putative PorY-PorX TCR system controls the secretion and maturation of virulence factors, as well as loci involved in the PorSS secretion system, which secretes proteinases, i.e., gingipains, responsible for periodontal disease. Proteomic analysis (SILAC) was used to improve the microarray data, reverse-transcription PCR to verify the proteomic data, and primer extension assay to determine the promoter regions of specific PorX regulated loci. I was able to characterize multiple genetic loci regulated by this TCR system, many of which play an essential role in hemagglutination and host-cell adhesion, and likely contribute to virulence in this bacterium. Enteric Gram-negative bacteria must withstand many host defenses such as digestive enzymes, low pH, and antimicrobial peptides (AMPs). The CpxR-CpxA TCR system of E. coli has been extensively characterized and shown to be required for protection against AMPs. Most recently, this TCR system has been shown to up-regulate the rfe-rff operon which encodes genes involved in the production of enterobacterial common antigen (ECA), and confers protection against a variety of AMPs. In this study, I utilized primer extension and DNase I footprinting to determine how CpxR regulates the ECA operon. My findings suggest that CpxR modulates transcription by directly binding to the rfe promoter. Multiple genetic and biochemical approaches were used to demonstrate that specific TCR systems contribute to regulation of virulence factors and resistance to host defenses in P. gingivalis and E. coli, respectively. Understanding these genetic circuits provides insight into strategies for pathogenesis and resistance to host defenses in Gram negative bacterial pathogens. Finally, these data provide compelling potential molecular targets for therapeutics to treat P. gingivalis and E. coli infections.
ContributorsLeonetti, Cori (Author) / Shi, Yixin (Thesis advisor) / Stout, Valerie (Committee member) / Nickerson, Cheryl (Committee member) / Sandrin, Todd (Committee member) / Arizona State University (Publisher)
Created2013
Description
This study focused on investigating the ability of a polymeric-enhanced high-tenacity fabric composite called CarbonFlex to mitigate damages from multi-natural hazards, which are earthquakes and tornadoes, in wood-framed structures. Typically, wood-framed shear wall is a seismic protection system used in low-rise wood structures. It is well-known that the main energy dissipation of the system is its fasteners (nails) which are not enough to dissipate energy leading to decreasing of structure's integrity. Moreover, wood shear walls could not sustain their stiffness after experiencing moderate wall drift which made them susceptible to strong aftershocks. Therefore, CarbonFlex shear wall system was proposed to be used in the wood-framed structures. Seven full-size CarbonFlex shear walls and a CarbonFlex wrapped structures were tested. The results were compared to those of conventional wood-framed shear walls and a wood structure. The comparisons indicated that CarbonFlex specimens could sustain their strength and fully recover their initial stiffness although they experienced four percent story drift while the stiffness of the conventional structure dramatically degraded. This indicated that CarbonFlex shear wall systems provided a better seismic protection to wood-framed structures. To evaluate capability of CarbonFlex to resist impact damages from wind-borne debris in tornadoes, several debris impact tests of CarbonFlex and a carbon fiber reinforced storm shelter's wall panels were conducted. The results showed that three CarbonFlex wall panels passed the test at the highest debris impact speed and the other two passed the test at the second highest speed while the carbon fiber panel failed both impact speeds.
ContributorsDhiradhamvit, Kittinan (Author) / Attard, Thomas L (Thesis advisor) / Fafitis, Apostolos (Thesis advisor) / Neithalath, Narayanan (Committee member) / Thomas, Benjamin (Committee member) / Arizona State University (Publisher)
Created2013