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Description
Enzyme Replacement Therapy (ERT) is a treatment often used for patients with disorders that affect the production of various enzymes within the body, such as Cystic Fibrosis and Fabry Disease. ERT involves the use of artificially-produced enzymes, which can be derived from humans, pigs, and bacteria. Generally, enzymes derived from

Enzyme Replacement Therapy (ERT) is a treatment often used for patients with disorders that affect the production of various enzymes within the body, such as Cystic Fibrosis and Fabry Disease. ERT involves the use of artificially-produced enzymes, which can be derived from humans, pigs, and bacteria. Generally, enzymes derived from porcine and bacterial sources are much less expensive and more accessible than those derived from a human source. This, and the ethical implications that porcine enzymes carry, make the decision of choosing treatment simple to some and complex to others. Ethically, human-derived enzymes are often considered more ethical, while not conflicting with religious beliefs and practices as porcine-derived enzymes do.
In order to further compare porcine and human-derived enzymes, a determination of the enzyme effectiveness was done via digestion simulation. The digestion for both the human and porcine-derived enzymes consisted of three steps: oral, gastric, and intestinal. After the digestion, the absorbance for each enzyme class as well as a dilution curve of the formula used was read and recorded. Using the standard dilution curve and the absorbance values for each unknown, the formula and thus enzyme concentration that was lost through the reaction was able to be calculated.
The effectiveness of both the human and porcine enzymes, determined by the percent of formula lost, was 18.2% and 19.7%, respectively, with an error of 0.6% from the spectrophotometer, and an error of about 10% from the scale used for measuring the enzymes. This error was likely due to the small mass required of the enzymes and can be prevented in the future by performing the experiment at a larger scale.
ContributorsBlevins, Brianna R (Author) / Martin, Thomas (Thesis director) / McILwraith, Heide (Committee member) / College of Integrative Sciences and Arts (Contributor) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
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Description

Toy hacks modify commercially available toys to be more easily used by people with motor disabilities, and donate them to schools, families, or toy libraries. Switch-adapting a toy adds an audio jack to allow an assistive technology (AT) switch to be plugged in. Switch-adapted toys help children develop essential skills

Toy hacks modify commercially available toys to be more easily used by people with motor disabilities, and donate them to schools, families, or toy libraries. Switch-adapting a toy adds an audio jack to allow an assistive technology (AT) switch to be plugged in. Switch-adapted toys help children develop essential skills through play. Hacking toys is helpful because toys that come with AT switches are often significantly more expensive than their unadapted counterparts. Toy hacks are also an opportunity to teach and practice engineering skills such as soldering and technical problem solving. Many resources are available online to assist makers with hosting toy hacks, but most of them lack information on holding the event. To fill this gap, the authors created a toy hack guide website, drawing from experience hosting two toy hacks. It walks users through steps like choosing the size of the event, the materials that need to be purchased, and connects them to other existing resources. In the future, it will be used to help people host more successful toy hacks.

ContributorsBushroe, Isabella (Author) / Koehl, Bridget (Co-author) / Frank, Daniel (Thesis director) / Brunhaver, Samantha (Committee member) / Martin, Thomas (Committee member) / Barrett, The Honors College (Contributor) / School of Art (Contributor) / Engineering Programs (Contributor)
Created2022-05
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Description

Toy hacks modify commercially available toys to be more easily used by people with motor disabilities, and donate them to schools, families, or toy libraries. Switch-adapting a toy adds an audio jack to allow an assistive technology (AT) switch to be plugged in. Switch-adapted toys help children develop essential skills

Toy hacks modify commercially available toys to be more easily used by people with motor disabilities, and donate them to schools, families, or toy libraries. Switch-adapting a toy adds an audio jack to allow an assistive technology (AT) switch to be plugged in. Switch-adapted toys help children develop essential skills through play. Hacking toys is helpful because toys that come with AT switches are often significantly more expensive than their unadapted counterparts. Toy hacks are also an opportunity to teach and practice engineering skills such as soldering and technical problem solving. Many resources are available online to assist makers with hosting toy hacks, but most of them lack information on holding the event. To fill this gap, the authors created a toy hack guide website, drawing from experience hosting two toy hacks. It walks users through steps like choosing the size of the event, the materials that need to be purchased, and connects them to other existing resources. In the future, it will be used to help people host more successful toy hacks.

ContributorsKoehl, Bridget (Author) / Bushroe, Isabella (Co-author) / Frank, Daniel (Thesis director) / Brunhaver, Samantha (Committee member) / Martin, Thomas (Committee member) / Barrett, The Honors College (Contributor) / Engineering Programs (Contributor)
Created2022-05