Matching Items (25)
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- All Subjects: Linguistics
- All Subjects: Synthetic Biology
- Creators: Matsuda, Aya
- Status: Published
Description
This study investigates how university instructors from various disciplines at a large, comprehensive university in the United States evaluate different varieties of English from countries considered "outer circle" (OC) countries, formerly colonized countries where English has been transplanted and is now used unofficially and officially to varying degrees. The study was designed to address two gaps in the research: (1) how instructors in increasingly internationalized U.S. universities evaluate different written varieties of English, since many international students may be writing in an L1 other than American English, and (2) how instructors' first language and/or disciplinary backgrounds appear to affect their evaluations. Through a comparison of rankings and qualitative analysis of interview data, the study examines whether the participating instructors value the same features and characteristics in writing, such as text and organization features, found in American English and varieties of OC written English. In addition, it examines whether one's first or native language or one's disciplinary training affects the perception and evaluation of these particular varieties of English. This study showed that what is currently valued and expected by instructors from various disciplines in U.S. universities is what may be identified as an "American" style of writing; participants expected an organization providing a clear purpose up front, including paragraphs of a certain length, and containing sentences perceived as more direct and succinct. In addition, given the overall agreement on the element of good writing demonstrated in how composition and content area professors ranked the writing samples, my study suggests that what is being taught in composition is preparing student for the writing expected in content area classes. Last, my findings add to World Englishes (WE) research by adding a writing component to WE attitudinal research studies, which have previously focused on oral production. Almost equal numbers of Native and Non-Native English Speakers (NESs and NNESs) participated, and the NNESs appeared more tolerant of different varieties, unlike the preference for inner circle norms noted in previous studies. This study, therefore, has implications for writing research and instruction at U.S. colleges and universities, as well as informing the field of World Englishes.
ContributorsCollier, Lizabeth C (Author) / Matsuda, Aya (Thesis advisor) / Wiley, Terrence (Committee member) / Mccarty, Teresa (Committee member) / Friedrich, Patricia (Committee member) / Arizona State University (Publisher)
Created2014
Description
This study investigates the uses of English in advertising in Brazil and the attitudes of Brazilians towards the use of different difficulty levels of English in advertising. Using a two part, mixed-methods approach, drawing from quantitative and qualitative methods, I utilized a corpus study to examine English uses in Brazilian magazines and a survey to investigate the difficulty of English slogans as a determinant for people's attitudes towards English in advertising. For the first part, three major Brazilian news magazines, Veja, Época, and ISTOÉ were used. From three issues of each magazine, results showed that 57% of the advertisements in all nine magazines contained English in different parts of the advertisements, with most occurrences in the product name, followed by the body copy, headline, subheadline, and slogan. English was used to advertise a number of different product types, but was especially used for advertising cars, electronics, events, and banks. It was also found that the majority of English was used for its symbolic representations of modernity, prestige, globalization, and reliability. Using a survey for the second part of the study, I investigated how Brazilian participants judged four advertisements that featured English slogans that were comparable to slogans judged to be easy or difficult to understand in a similar study conducted by Hornikx, van Meurs, and de Boer (2010). Participants were offered attitudinal choices to mark off on a 4-point Likert scale, where they indicated their attitudes towards the English slogans provided. They were also asked to determine if they understood the slogans and to translate them to indicate their actual understanding of the slogans. Participants showed more positive attitudes towards the uses of English than negative attitudes. The survey provided evidence that with the very low numbers of correctly translated slogans, many participants believed they understood the slogans, which could prove to be more of an indicator of positive attitudes than their actual understanding of the slogans. This project provides an example from one Expanding Circle context touched by the far-reaching influences of World Englishes.
ContributorsMontes, Amanda Lira Gordenstein (Author) / Friedrich, Patricia M (Thesis advisor) / Matsuda, Aya (Committee member) / Lafford, Barbara (Committee member) / Anokye, Akua Duku (Committee member) / Arizona State University (Publisher)
Created2014
Description
The present study investigates some of the different ways in which English has been conceptualized in Brazil since the beginning of intensified globalization in the 1990s, and proposes how such conceptualizations relate to sociocultural, political and historical phenomena in the country. To this end, central texts (governmental documents, musical lyrics, cultural messages, educational policies, and language school commercials) of three domains of language regulation and use (political discourse, pop culture, and English language teaching) were examined through discourse analytical tools, text mapping, and content analyses. The investigation of each domain was supplemented by analyses of additional data (media texts, artistic work, and teacher interviews) that either confirmed or problematized results. Findings showed that the symbolic meanings of English in Brazil are caught in a heteroglossic web of discourses, which reflect diverse understandings of global processes, of the spread of English, and of Brazil itself. Tensions between authoritative and internally persuasive discourses, and between centripetal and centrifugal forces are revealed not only across different texts and realms - as reported in studies of English in other contexts - but also within domains, and within the discourses of the same people and institutions. It is argued that legislative authority, the role of the state, and the contradictions between discourses of mobility and empowerment and unsuccessful educational practices play a central role in the way English is understood and experienced in Brazil, confirming previous claims of an identity crisis, and revealing other crises of power, democracy, politics, and education. The study adds to the literature on English conceptualizations by bringing an understanding of the case of Brazil, which has not been as extensively investigated as other contexts. Moreover, the individual analyses presented bring new perspectives on the political discourses that have attempted to regulate loanword use in Brazil, and on the nature of language teaching in the country, besides emphasizing the role of pop culture in the understanding of English in that context. Further implications include the discussion of how the study of the spread of English may connect with different understandings of globalization, and the presentation of how the results contribute to language education.
ContributorsFigueiredo, Eduardo Henrique Diniz de (Author) / Friedrich, Patricia (Committee member) / Matsuda, Aya (Committee member) / Gee, James (Committee member) / Arizona State University (Publisher)
Created2012
Description
The portability of genetic tools from one organism to another is a cornerstone of synthetic biology. The shared biological language of DNA-to-RNA-to-protein allows for expression of polypeptide chains in phylogenetically distant organisms with little modification. The tools and contexts are diverse, ranging from catalytic RNAs in cell-free systems to bacterial proteins expressed in human cell lines, yet they exhibit an organizing principle: that genes and proteins may be treated as modular units that can be moved from their native organism to a novel one. However, protein behavior is always unpredictable; drop-in functionality is not guaranteed.
My work characterizes how two different classes of tools behave in new contexts and explores methods to improve their functionality: 1. CRISPR/Cas9 in human cells and 2. quorum sensing networks in Escherichia coli.
1. The genome-editing tool CRISPR/Cas9 has facilitated easily targeted, effective, high throughput genome editing. However, Cas9 is a bacterially derived protein and its behavior in the complex microenvironment of the eukaryotic nucleus is not well understood. Using transgenic human cell lines, I found that gene-silencing heterochromatin impacts Cas9’s ability to bind and cut DNA in a site-specific manner and I investigated ways to improve CRISPR/Cas9 function in heterochromatin.
2. Bacteria use quorum sensing to monitor population density and regulate group behaviors such as virulence, motility, and biofilm formation. Homoserine lactone (HSL) quorum sensing networks are of particular interest to synthetic biologists because they can function as “wires” to connect multiple genetic circuits. However, only four of these networks have been widely implemented in engineered systems. I selected ten quorum sensing networks based on their HSL production profiles and confirmed their functionality in E. coli, significantly expanding the quorum sensing toolset available to synthetic biologists.
My work characterizes how two different classes of tools behave in new contexts and explores methods to improve their functionality: 1. CRISPR/Cas9 in human cells and 2. quorum sensing networks in Escherichia coli.
1. The genome-editing tool CRISPR/Cas9 has facilitated easily targeted, effective, high throughput genome editing. However, Cas9 is a bacterially derived protein and its behavior in the complex microenvironment of the eukaryotic nucleus is not well understood. Using transgenic human cell lines, I found that gene-silencing heterochromatin impacts Cas9’s ability to bind and cut DNA in a site-specific manner and I investigated ways to improve CRISPR/Cas9 function in heterochromatin.
2. Bacteria use quorum sensing to monitor population density and regulate group behaviors such as virulence, motility, and biofilm formation. Homoserine lactone (HSL) quorum sensing networks are of particular interest to synthetic biologists because they can function as “wires” to connect multiple genetic circuits. However, only four of these networks have been widely implemented in engineered systems. I selected ten quorum sensing networks based on their HSL production profiles and confirmed their functionality in E. coli, significantly expanding the quorum sensing toolset available to synthetic biologists.
ContributorsDaer, René (Author) / Haynes, Karmella (Thesis advisor) / Brafman, David (Committee member) / Nielsen, David (Committee member) / Kiani, Samira (Committee member) / Arizona State University (Publisher)
Created2017
Description
This study explores the online recruitment and mobilization of followers in a social movement. In this study, I identify and analyze how certain narratives were produced, distributed and recirculated online by a social movement organization that depicted players in the movement in ways that engaged followers in actions of advocacy and support. Also, I examine how particular narratives were taken up, negotiated, amplified, and distributed by online supporters who eventually become co-tellers of the narrative and ultimately advocates on behalf of the social movement. By examining a selection of media statements, open letters, protest speeches, blogs, videos and pictures, I show how online practices might contribute to inspiring and mobilizing action or responses from a large number of followers. Data include selected excerpts from an online social movement that began in Norway in 2015 and later gathered momentum and strength outside of Norway and Europe. This multi-modal analysis of digital practices demonstrates how collaboratively produced narratives (e.g., of suffering, sorrow, persecution or resilience) emerge and gain traction in the digital space, the relationship between the temporal and spatial dimensions of narrative, and the role of collective memory in building a sense of community and shared identity. Demonstrating the dialogic and interactional dimensions of meaning-making processes, this case study informs how we might theorize and understand the role of identity and narrative in the emergence and amplification of social movements.
ContributorsPaulesc, Julieta Cristina (Author) / Warriner, Doris S (Thesis advisor) / Matsuda, Aya (Committee member) / Prior, Matthew (Committee member) / Arizona State University (Publisher)
Created2019
Description
For this dissertation, teacher linguistic awareness (TLingA) involves teacher linguistic knowledge, teacher language awareness (TLA), and teacher cognition for second language (L2) teachers. Teacher linguistic knowledge is an understanding of how language functions and is compiled within the different areas of linguistics. And TLA is the knowledge that educators possess of the structural and fundamental system of language. Both help L2 teachers with different aspects of teaching. Additionally, teacher cognition involves what teachers know, their beliefs, and thought processes. Lastly, TLingA includes the conscious application of teacher linguistic knowledge.
In order to understand how strong of a role linguistics plays in language instruction, I evaluate how language teachers use their linguistic knowledge, and what factors affect the application of that knowledge. This paper aims to fill this gap in understanding how much and what factors affect L2 teachers’ application of linguistic knowledge by interviewing L2 teachers at an intensive English program at a university in the Southwestern United States. To do so, the study uses interviews with open-ended questions involving hypothetical teaching scenarios that probe different areas of linguistics: phonology, grammar, pragmatics, and sociolinguistics.
The general findings suggest that teachers use their linguistic knowledge and awareness in their teaching: such as, with sociolinguistics, in how they control the classroom and interact with students; with phonology, in how they teach pronunciation; with grammar, in how they edit students’ writing and meet with students about their writing; and with pragmatics, in how they teach vocabulary usage and formal requests. Additionally, the results suggest that years of experience appear to be the largest factor in the application of linguistic knowledge and that contextual factors, like time and curriculum goals, also play a role. Moreover, in relation to teacher cognition, how a L2 teacher conceptualizes or defines linguistic terms also seemed to affect their awareness of the application of linguistic knowledge. In conclusion, it appears that L2 teachers’ linguistic knowledge and TLingA help them to evaluate their students’ needs and influence their lesson planning.
In order to understand how strong of a role linguistics plays in language instruction, I evaluate how language teachers use their linguistic knowledge, and what factors affect the application of that knowledge. This paper aims to fill this gap in understanding how much and what factors affect L2 teachers’ application of linguistic knowledge by interviewing L2 teachers at an intensive English program at a university in the Southwestern United States. To do so, the study uses interviews with open-ended questions involving hypothetical teaching scenarios that probe different areas of linguistics: phonology, grammar, pragmatics, and sociolinguistics.
The general findings suggest that teachers use their linguistic knowledge and awareness in their teaching: such as, with sociolinguistics, in how they control the classroom and interact with students; with phonology, in how they teach pronunciation; with grammar, in how they edit students’ writing and meet with students about their writing; and with pragmatics, in how they teach vocabulary usage and formal requests. Additionally, the results suggest that years of experience appear to be the largest factor in the application of linguistic knowledge and that contextual factors, like time and curriculum goals, also play a role. Moreover, in relation to teacher cognition, how a L2 teacher conceptualizes or defines linguistic terms also seemed to affect their awareness of the application of linguistic knowledge. In conclusion, it appears that L2 teachers’ linguistic knowledge and TLingA help them to evaluate their students’ needs and influence their lesson planning.
ContributorsFoltz, Karen (Author) / Matsuda, Aya (Thesis advisor) / Renaud, Claire (Thesis advisor) / James, Mark (Committee member) / Arizona State University (Publisher)
Created2018
Description
Synthetic manipulation of chromatin dynamics has applications for medicine, agriculture, and biotechnology. However, progress in this area requires the identification of design rules for engineering chromatin systems. In this thesis, I discuss research that has elucidated the intrinsic properties of histone binding proteins (HBP), and apply this knowledge to engineer novel chromatin binding effectors. Results from the experiments described herein demonstrate that the histone binding domain from chromobox protein homolog 8 (CBX8) is portable and can be customized to alter its endogenous function. First, I developed an assay to identify engineered fusion proteins that bind histone post translational modifications (PTMs) in vitro and regulate genes near the same histone PTMs in living cells. This assay will be useful for assaying the function of synthetic histone PTM-binding actuators and probes. Next, I investigated the activity of a novel, dual histone PTM binding domain regulator called Pc2TF. I characterized Pc2TF in vitro and in cells and show it has enhanced binding and transcriptional activation compared to a single binding domain fusion called Polycomb Transcription Factor (PcTF). These results indicate that valency can be used to tune the activity of synthetic histone-binding transcriptional regulators. Then, I report the delivery of PcTF fused to a cell penetrating peptide (CPP) TAT, called CP-PcTF. I treated 2D U-2 OS bone cancer cells with CP-PcTF, followed by RNA sequencing to identify genes regulated by CP-PcTF. I also showed that 3D spheroids treated with CP-PcTF show delayed growth. This preliminary work demonstrated that an epigenetic effector fused to a CPP can enable entry and regulation of genes in U-2 OS cells through DNA independent interactions. Finally, I described and validated a new screening method that combines the versatility of in vitro transcription and translation (IVTT) expressed protein coupled with the histone tail microarrays. Using Pc2TF as an example, I demonstrated that this assay is capable of determining binding and specificity of a synthetic HBP. I conclude by outlining future work toward engineering HBPs using techniques such as directed evolution and rational design. In conclusion, this work outlines a foundation to engineer and deliver synthetic chromatin effectors.
ContributorsTekel, Stefan (Author) / Haynes, Karmella (Thesis advisor) / Mills, Jeremy (Committee member) / Caplan, Michael (Committee member) / Brafman, David (Committee member) / Arizona State University (Publisher)
Created2019
Description
Transgene expression in mammalian cells has been shown to meet resistance in the form of silencing due to chromatin buildup within the cell. Interactions of proteins with chromatin modulate gene expression profiles. Synthetic Polycomb transcription factor (PcTF) variants have the potential to reactivate these silence transgenes as shown in Haynes & Silver 2011. PcTF variants have been constructed via TypeIIS assembly to further investigate this ability to reactive transgenes. Expression in mammalian cells was confirmed via fluorescence microscopy and red fluorescent protein (RFP) expression in cell lysate. Examination of any variation in conferment of binding strength of homologous Polycomb chromodomains (PCDs) to its trimethylated lysine residue target on histone three (H3K27me3) was investigated using a thermal shift assay. Results indicate that PcTF may not be a suitable protein for surveying with SYPRO Orange, a dye that produces a detectable signal when exposed to the hydrophobic domains of the melting protein. A cell line with inducible silencing of a chemiluminescent protein was used to determine the effects PcTF variants had on gene reactivation. Results show down-regulation of the target reporter gene. We propose this may be due to PcTF not binding to its target; this would cause PcTF to deplete transcriptional machinery in the nucleus. Alternatively, the CMV promoter could be sequestering transcriptional machinery in its hyperactive transcription of PcTF leading to widespread down-regulation. Finally, the activation domain used may not be appropriate for this cell type. Future PcTF variants will address these hypotheses by including multiple Polycomb chromodomains (PCDs) to alter the binding dynamics of PcTF to its target, and by incorporating alternative promoters and activation domains.
ContributorsGardner, Cameron Lee (Author) / Haynes, Karmella (Thesis director) / Stabenfeldt, Sarah (Committee member) / Barrett, The Honors College (Contributor) / Department of Finance (Contributor) / Harrington Bioengineering Program (Contributor)
Created2015-05
Description
Currently in synthetic biology only the Las, Lux, and Rhl quorum sensing pathways have been adapted for broad engineering use. Quorum sensing allows a means of cell to cell communication in which a designated sender cell produces quorum sensing molecules that modify gene expression of a designated receiver cell. While useful, these three quorum sensing pathways exhibit a nontrivial level of crosstalk, hindering robust engineering and leading to unexpected effects in a given design. To address the lack of orthogonality among these three quorum sensing pathways, previous scientists have attempted to perform directed evolution on components of the quorum sensing pathway. While a powerful tool, directed evolution is limited by the subspace that is defined by the protein. For this reason, we take an evolutionary biology approach to identify new orthogonal quorum sensing networks and test these networks for cross-talk with currently-used networks. By charting characteristics of acyl homoserine lactone (AHL) molecules used across quorum sensing pathways in nature, we have identified favorable candidate pathways likely to display orthogonality. These include Aub, Bja, Bra, Cer, Esa, Las, Lux, Rhl, Rpa, and Sin, which we have begun constructing and testing. Our synthetic circuits express GFP in response to a quorum sensing molecule, allowing quantitative measurement of orthogonality between pairs. By determining orthogonal quorum sensing pairs, we hope to identify and adapt novel quorum sensing pathways for robust use in higher-order genetic circuits.
ContributorsMuller, Ryan (Author) / Haynes, Karmella (Thesis director) / Wang, Xiao (Committee member) / Barrett, The Honors College (Contributor) / School of Mathematical and Statistical Sciences (Contributor) / Department of Chemistry and Biochemistry (Contributor) / School of Life Sciences (Contributor)
Created2015-05
Description
Synthetic biology is an emerging engineering disciple, which designs and controls biological systems for creation of materials, biosensors, biocomputing, and much more. To better control and engineer these systems, modular genetic components which allow for highly specific and high dynamic range genetic regulation are necessary. Currently the field struggles to demonstrate reliable regulators which are programmable and specific, yet also allow for a high dynamic range of control. Inspired by the characteristics of the RNA toehold switch in E. coli, this project attempts utilize artificial introns and complementary trans-acting RNAs for gene regulation in a eukaryote host, S. cerevisiae. Following modification to an artificial intron, splicing control with RNA hairpins was demonstrated. Temperature shifts led to increased protein production likely due to increased splicing due to hairpin loosening. Progress is underway to demonstrate trans-acting RNA interaction to control splicing. With continued development, we hope to provide a programmable, specific, and effective means for translational gene regulation in S. cerevisae.
ContributorsDorr, Brandon Arthur (Author) / Wang, Xiao (Thesis director) / Green, Alexander (Committee member) / Harrington Bioengineering Program (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05