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Cardiovascular disease is one of the most deadly outcomes of end stage renal disease. Bioelectrical impedance is a intriguing, yet unproven method of measuring fluid buildup in the heart, and is marketed as a early diagnostic tool for onset of cardiovascular disease. In this study, selenium supplements were given to

Cardiovascular disease is one of the most deadly outcomes of end stage renal disease. Bioelectrical impedance is a intriguing, yet unproven method of measuring fluid buildup in the heart, and is marketed as a early diagnostic tool for onset of cardiovascular disease. In this study, selenium supplements were given to a cohort of dialysis patients in the Phoenix metro area and their fluid tolerance was measured with thoracic biolectrical impedance. BNP was used as a correlate to see if bioelectrical impedance was correlated with heart disease. The study found no correlation between BNP and bioelectrical impedance and thus was not an accurate diagnostic tool in a medical setting.
ContributorsBrown, Patrick Michael (Author) / Johnston, Carol (Thesis director) / Orchinik, Miles (Committee member) / Tingey, Michael (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor) / School of Historical, Philosophical and Religious Studies (Contributor)
Created2013-05
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Ephemeral and intermittent streams are valuable sources of surface water support in the arid ecosystems of the Southwestern United States. These streams account for over 80% of the streams in the American Southwest and their importance has been indicated in many studies. Ephemeral and intermittent streams support a wide range

Ephemeral and intermittent streams are valuable sources of surface water support in the arid ecosystems of the Southwestern United States. These streams account for over 80% of the streams in the American Southwest and their importance has been indicated in many studies. Ephemeral and intermittent streams support a wide range of plant and animal species in both continuous and episodic fashions. This study aimed to gain a better understanding of the relationship between streamflow permanence and patterns of biomass and secondary production of the riparian fauna these ecosystems support. This was accomplished through a yearlong survey in the Huachuca Mountains of Southeastern, Arizona where macroinvertebrates were collected at various sites along a gradient of streamflow permanence before, during, and after the three month monsoon season that supplies most of the annual rainfall in this region. The results of my surveys indicate that 1) Sites characterized by low streamflow permanence were more responsive to changes in precipitation than sites characterized by relatively high streamflow permanence 2) In ephemeral streams, there is a significant peak in terrestrial macroinvertebrate production and biomass both during and after the monsoon season 3) streamflow permanence may convey consistent but not exceptional secondary production whereas seasonality in rainfall may convey exceptional but episodic secondary production—more so in sites where streamflow is not consistent.
ContributorsMcCartin, Michael Patrick (Author) / Sabo, John (Thesis director) / Stromberg, Juliet (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor)
Created2014-05
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We examined the evolutionary morphological responses of Drosophila melanogaster that had evolved at constant cold (16°), constant hot (25°C), and fluctuating (16° and 25°C). Flies that were exposed to the constant low mean temperature developed larger thorax, wing, and cell sizes than those exposed to constant high mean temperatures. Males

We examined the evolutionary morphological responses of Drosophila melanogaster that had evolved at constant cold (16°), constant hot (25°C), and fluctuating (16° and 25°C). Flies that were exposed to the constant low mean temperature developed larger thorax, wing, and cell sizes than those exposed to constant high mean temperatures. Males and females both responded similarly to thermal treatments in average wing and cell size. The resulting cell area for a given wing size in thermal fluctuating populations remains unclear and remains a subject for future research.
ContributorsAdrian, Gregory John (Author) / Angilletta, Michael (Thesis director) / Harrison, Jon (Committee member) / Rusch, Travis (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor)
Created2015-05
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Studies of animal contests often focus solely on a single static measurement of fighting ability, such as the size or the strength of the individual. However, recent studies have highlighted the importance of individual variation in the dynamic behaviors used during a fight, such as, assessment strategies, decision making, and

Studies of animal contests often focus solely on a single static measurement of fighting ability, such as the size or the strength of the individual. However, recent studies have highlighted the importance of individual variation in the dynamic behaviors used during a fight, such as, assessment strategies, decision making, and fine motor control, as being strong predictors of the outcome of aggression. Here, I combined morphological and behavioral data to discover how these features interact during aggressing interactions in male virile crayfish, Faxonius virilis. I predicted that individual variation in behavioral skill for decision making (i.e., number of strikes thrown), would determine the outcome of contest success in addition to morphological measurements (e.g. body size, relative claw size). To evaluate this prediction, I filmed staged territorial interactions between male F. virilis and later analyzed trial behaviors (e.g. strike, pinches, and bout time) and aggressive outcomes. I found very little support for skill to predict win/loss outcome in trials. Instead, I found that larger crayfish engaged in aggression for longer compared to smaller crayfish, but that larger crayfish did not engage in a greater number of claw strikes or pinches when controlling for encounter duration. Future studies should continue to investigate the role of skill, by using finer-scale techniques such as 3D tracking software, which could track advanced measurements (e.g. speed, angle, and movement efficiency). Such studies would provide a more comprehensive understanding of the relative influence of fighting skill technique on territorial contests.

ContributorsNguyen, Phillip Huy (Author) / Angilletta, Michael (Thesis director) / McGraw, Kevin (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
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Land management practices such as domestic animal grazing can alter plant communities via changes in soil structure and chemistry, species composition, and plant nutrient content. These changes can affect the abundance and quality of plants consumed by insect herbivores with consequent changes in population dynamics. These population changes can translate

Land management practices such as domestic animal grazing can alter plant communities via changes in soil structure and chemistry, species composition, and plant nutrient content. These changes can affect the abundance and quality of plants consumed by insect herbivores with consequent changes in population dynamics. These population changes can translate to massive crop damage and pest control costs. My dissertation focused on Oedaleus asiaticus, a dominant Asian locust, and had three main objectives. First, I identified morphological, physiological, and behavioral characteristics of the migratory ("brown") and non-migratory ("green") phenotypes. I found that brown morphs had longer wings, larger thoraxes and higher metabolic rates compared to green morphs, suggesting that developmental plasticity allows greater migratory capacity in the brown morph of this locust. Second, I tested the hypothesis of a causal link between livestock overgrazing and an increase in migratory swarms of O. asiaticus. Current paradigms generally assume that increased plant nitrogen (N) should enhance herbivore performance by relieving protein-limitation, increasing herbivorous insect populations. I showed, in contrast to this scenario, that host plant N-enrichment and high protein artificial diets decreased the size and viability of O. asiaticus. Plant N content was lowest and locust abundance highest in heavily livestock-grazed fields where soils were N-depleted, likely due to enhanced erosion and leaching. These results suggest that heavy livestock grazing promotes outbreaks of this locust by reducing plant protein content. Third, I tested for the influence of dietary imbalance, in conjunction with high population density, on migratory plasticity. While high population density has clearly been shown to induce the migratory morph in several locusts, the effect of diet has been unclear. I found that locusts reared at high population density and fed unfertilized plants (i.e. high quality plants for O. asiaticus) had the greatest migratory capacity, and maintained a high percent of brown locusts. These results did not support the hypothesis that poor-quality resources increased expression of migratory phenotypes. This highlights a need to develop new theoretical frameworks for predicting how environmental factors will regulate migratory plasticity in locusts and perhaps other insects.
ContributorsCease, Arianne (Author) / Harrison, Jon (Thesis advisor) / Elser, James (Thesis advisor) / DeNardo, Dale (Committee member) / Quinlan, Michael (Committee member) / Sabo, John (Committee member) / Arizona State University (Publisher)
Created2012
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The southwestern willow flycatcher (Empidonax traillii extimus) is listed as an endangered species throughout its range in the southwestern United States. Little is known about its sub-population spatial structure and how this impacts its population viability. In conjunction with being listed as endangered, a recovery plan was produced by the

The southwestern willow flycatcher (Empidonax traillii extimus) is listed as an endangered species throughout its range in the southwestern United States. Little is known about its sub-population spatial structure and how this impacts its population viability. In conjunction with being listed as endangered, a recovery plan was produced by the US Fish and Wildlife Service, with recovery units (sub-populations) roughly based on major river drainages. In the interest of examining this configuration of sub-populations and their impact on the measured population viability, I applied a multivariate auto-regressive state-space model to a spatially extensive time series of abundance data for the southwestern willow flycatcher over the period spanning 1995-2010 estimating critical growth parameters, correlation in environmental stochasticity or "synchronicity" between sub-populations (recovery units) and extinction risk of the sub-populations and the whole. The model estimates two parameters, the mean and variance of annual growth rate. Of the models I tested, I found the strongest support for a population model in which three of the recovery units were grouped (the Lower Colorado, Gila Basin, and Rio Grande recovery units) while keeping all others separate. This configuration has 6.6 times more support for the observed data than a configuration assigning each recovery unit to a separate sub-population, which is how they are circumscribed in the recovery plan. Given the best model, the mean growth rate is -0.0234 (CI95 -0.0939, 0.0412) with a variance of 0.0597 (CI95 0.0115, 0.1134). This growth rate is not significantly different from zero and this is reflected in the low potential for quasi-extinction. The cumulative probability of the population experiencing at least an 80% decline from current levels within 15 years for some sub-populations were much higher (range: 0.129-0.396 for an 80% decline). These results suggest that the rangewide population has a low risk of extinction in the next 15 years and that the formal recovery units specified by the original recovery plan do not correspond to proper sub-population units as defined by population synchrony.
ContributorsDockens, Patrick E. T. (Author) / Sabo, John (Thesis advisor) / Stromberg, Juliet (Committee member) / Fenichel, Eli (Committee member) / Arizona State University (Publisher)
Created2012
Description

The Northern Gulf of California is characterized by an extreme tidal range and temperature fluctuations between seasons, as well as a large variation in microhabitats along its shoreline. As a result, the intertidal regions exhibit a diverse and distinct collection of species that have adapted to these environmental conditions, with

The Northern Gulf of California is characterized by an extreme tidal range and temperature fluctuations between seasons, as well as a large variation in microhabitats along its shoreline. As a result, the intertidal regions exhibit a diverse and distinct collection of species that have adapted to these environmental conditions, with roughly 4.6 percent being endemic. Minimal knowledge of these ecosystems existed until the 1940’s, when the renowned author John Steinbeck accompanied marine biologist Edward Ricketts on an expedition with the purpose of documenting the biodiversity of the Sea of Cortez. Today, the majority of research in the Northern Gulf of California is directed by CEDO, the Intercultural Center for the Study of Deserts and Oceans. The purpose of this project is to compile a literature review of research on the intertidal areas of the Northern Gulf and produce an illustrated brochure that educates beach visitors on local biodiversity as a collaboration with CEDO and the Clean Beaches Committee of Puerto Peñasco. This brochure aims to increase respect and appreciation for these species, as increased tourism over the past few decades has led to detrimental effects on the ecosystem. Additionally, it serves to promote the success of the Blue Flag certification of El Mirador beach in front of Manny’s Beach Club.

ContributorsPotter, Jessica Noel (Co-author) / Potter, Jessica (Co-author) / Neuer, Susanne (Thesis director) / Mangin, Katrina (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
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Introduction: Human papillomavirus (HPV) infection is seen in up to 90% of cases of cervical cancer, the third leading cancer cause of death in women. Current HPV screening focuses on only two HPV types and covers roughly 75% of HPV-associated cervical cancers. A protein based assay to test for antibody

Introduction: Human papillomavirus (HPV) infection is seen in up to 90% of cases of cervical cancer, the third leading cancer cause of death in women. Current HPV screening focuses on only two HPV types and covers roughly 75% of HPV-associated cervical cancers. A protein based assay to test for antibody biomarkers against 98 HPV antigens from both high and low risk types could provide an inexpensive and reliable method to screen for patients at risk of developing invasive cervical cancer. Methods: 98 codon optimized, commercially produced HPV genes were cloned into the pANT7_cGST vector, amplified in a bacterial host, and purified for mammalian expression using in vitro transcription/translation (IVTT) in a luminescence-based RAPID ELISA (RELISA) assay. Monoclonal antibodies were used to determine immune cross-reactivity between phylogenetically similar antigens. Lastly, several protein characteristics were examined to determine if they correlated with protein expression. Results: All genes were successfully moved into the destination vector and 86 of the 98 genes (88%) expressed protein at an adequate level. A difference was noted in expression by gene across HPV types but no correlation was found between protein size, pI, or aliphatic index and expression. Discussion: Further testing is needed to express the remaining 12 HPV genes. Once all genes have been successfully expressed and purified at high concentrations, DNA will be printed on microscope slides to create a protein microarray. This microarray will be used to screen HPV-positive patient sera for antibody biomarkers that may be indicative of cervical cancer and precancerous cervical neoplasias.
ContributorsMeshay, Ian Matthew (Author) / Anderson, Karen (Thesis director) / Magee, Mitch (Committee member) / Katchman, Benjamin (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor)
Created2015-05
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Triops (Branchiopoda: Notostraca) and Streptocephalus (Branchiopoda: Anostraca) are two crustaceans which cohabitate in ephemeral freshwater pools. They both lay desiccation resistant eggs that disperse passively to new hydrologically isolated environments. The extent of genetic distance among regions and populations is of perennial interest in animals that live in such isolated

Triops (Branchiopoda: Notostraca) and Streptocephalus (Branchiopoda: Anostraca) are two crustaceans which cohabitate in ephemeral freshwater pools. They both lay desiccation resistant eggs that disperse passively to new hydrologically isolated environments. The extent of genetic distance among regions and populations is of perennial interest in animals that live in such isolated habitats. Populations in six natural ephemeral pool habitats located in two different regions of the Sonoran Desert and a transition area between the Sonoran and Chihuahuan Deserts were sampled. Sequences from Genbank were used for reference points in the determination of species as well as to further identify regional genetic distance within species. This study estimated the amount of within and between genetic distance of individuals from each region and population through the use of a neutral marker, cytochrome oxidase I (COI). We concluded that, although the method of passive dispersal may differ between the two genera, the differences do not results in different patterns of genetic distances between regions and populations. Furthermore, we only found the putative species, Triops longicaudatus "short", with enough distinct speciation. Although Triops longicaudatus "long" and Triops newberryi may be in the early stages of speciation, this study does not find enough support to conclude that they have separated.
ContributorsMurphy Jr., Patrick Joseph (Author) / Rutowski, Ronald (Thesis director) / Cartwright, Reed (Committee member) / Lessios, Nikos (Committee member) / School of Life Sciences (Contributor) / School of Human Evolution and Social Change (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
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This work examines one dimension of the effect that complex human transport systems have on the spread of Chikungunya Virus (CHIKV) in the Caribbean from 2013 to 2015. CHIKV is transmitted by mosquitos and its novel spread through the Caribbean islands provided a chance to examine disease transmission through complex

This work examines one dimension of the effect that complex human transport systems have on the spread of Chikungunya Virus (CHIKV) in the Caribbean from 2013 to 2015. CHIKV is transmitted by mosquitos and its novel spread through the Caribbean islands provided a chance to examine disease transmission through complex human transportation systems. Previous work by Cauchemez et al. had shown a simple distance-based model successfully predict CHIKV spread in the Caribbean using Markov chain Monte Carlo (MCMC) statistical methods. A MCMC simulation is used to evaluate different transportation methods (air travel, cruise ships, and local maritime traffic) for the primary transmission patterns through linear regression. Other metrics including population density to account for island size variation and dengue fever incidence rates as a proxy for vector control and health spending were included. Air travel and cruise travel were gathered from monthly passenger arrivals by island. Local maritime traffic is approximated with a gravity model proxy incorporating GDP-per-capita and distance and historic dengue rates were used for determine existing vector control measures for the islands. The Caribbean represents the largest cruise passenger market in the world, cruise ship arrivals were expected to show the strongest signal; however, the gravity model representing local traffic was the best predictor of infection routes. The early infected islands (<30 days) showed a heavy trend towards an alternate primary transmission but our consensus model able to predict the time until initial infection reporting with 94.5% accuracy for islands 30 days post initial reporting. This result can assist public health entities in enacting measures to mitigate future epidemics and provide a modelling basis for determining transmission modes in future CHIKV outbreaks.
ContributorsFries, Brendan F (Author) / Perrings, Charles (Thesis director) / Wilson Sayres, Melissa (Committee member) / Morin, Ben (Committee member) / School of Life Sciences (Contributor) / Department of Military Science (Contributor) / Barrett, The Honors College (Contributor)
Created2015-12