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Description
Extracellular vesicles (EVs) are membranous particles that are abundantly secreted in the circulation system by most cells and can be found in most biological fluids. Among different EV subtypes, exosomes are small particles (30 – 150 nm) that are generated through the double invagination of the lipid bilayer membrane of cell. Therefore, they mirror the cell membrane proteins and contain proteins, RNAs, and DNAs that can represent the phenotypic state of their cell of origin, hence considered promising biomarker candidates. Importantly, in most pathological conditions, such as cancer and infection, diseased cells secrete more EVs and the disease associated exosomes have shown great potential to serve as biomarkers for early diagnosis, disease staging, and treatment monitoring. However, using EVs as diagnostic or prognostic tools in the clinic is hindered by the lack of a rapid, sensitive, purification-free technique for their isolation and characterization. Developing standardized assays that can translate the emerging academic EV biomarker discoveries to clinically relevant procedures is a bottleneck that have slowed down advancements in medical research. Integrating widely known immunoassays with plasmonic sensors has shown the promise to detect minute amounts of antigen present in biological sample, based on changes of ambient optical refractive index, and achieve ultra-sensitivity. Plasmonic sensors take advantage of the enhanced interaction of electromagnetic radiations with electron clouds of plasmonic materials at the dielectric-metal interface in tunable wavelengths.
ContributorsAmrollahi, Pouy (Author) / Wang, Xiao (Thesis advisor) / Forzani, Erica (Committee member) / Hu, Tony Ye (Committee member) / Arizona State University (Publisher)
Created2020
Description
Imagery data has become important for civil infrastructure operation and
maintenance because imagery data can capture detailed visual information with high
frequencies. Computer vision can be useful for acquiring spatiotemporal details to
support the timely maintenance of critical civil infrastructures that serve society. Some
examples include: irrigation canals need to maintain the leaking sections to avoid water
loss; project engineers need to identify the deviating parts of the workflow to have the
project finished on time and within budget; detecting abnormal behaviors of air traffic
controllers is necessary to reduce operational errors and avoid air traffic accidents.
Identifying the outliers of the civil infrastructure can help engineers focus on targeted
areas. However, large amounts of imagery data bring the difficulty of information
overloading. Anomaly detection combined with contextual knowledge could help address
such information overloading to support the operation and maintenance of civil
infrastructures.
Some challenges make such identification of anomalies difficult. The first challenge is
that diverse large civil infrastructures span among various geospatial environments so
that previous algorithms cannot handle anomaly detection of civil infrastructures in
different environments. The second challenge is that the crowded and rapidly changing
workspaces can cause difficulties for the reliable detection of deviating parts of the
workflow. The third challenge is that limited studies examined how to detect abnormal
behaviors for diverse people in a real-time and non-intrusive manner. Using video andii
relevant data sources (e.g., biometric and communication data) could be promising but
still need a baseline of normal behaviors for outlier detection.
This dissertation presents an anomaly detection framework that uses contextual
knowledge, contextual information, and contextual data for filtering visual information
extracted by computer vision techniques (ADCV) to address the challenges described
above. The framework categorizes the anomaly detection of civil infrastructures into two
categories: with and without a baseline of normal events. The author uses three case
studies to illustrate how the developed approaches can address ADCV challenges in
different categories of anomaly detection. Detailed data collection and experiments
validate the developed ADCV approaches.
maintenance because imagery data can capture detailed visual information with high
frequencies. Computer vision can be useful for acquiring spatiotemporal details to
support the timely maintenance of critical civil infrastructures that serve society. Some
examples include: irrigation canals need to maintain the leaking sections to avoid water
loss; project engineers need to identify the deviating parts of the workflow to have the
project finished on time and within budget; detecting abnormal behaviors of air traffic
controllers is necessary to reduce operational errors and avoid air traffic accidents.
Identifying the outliers of the civil infrastructure can help engineers focus on targeted
areas. However, large amounts of imagery data bring the difficulty of information
overloading. Anomaly detection combined with contextual knowledge could help address
such information overloading to support the operation and maintenance of civil
infrastructures.
Some challenges make such identification of anomalies difficult. The first challenge is
that diverse large civil infrastructures span among various geospatial environments so
that previous algorithms cannot handle anomaly detection of civil infrastructures in
different environments. The second challenge is that the crowded and rapidly changing
workspaces can cause difficulties for the reliable detection of deviating parts of the
workflow. The third challenge is that limited studies examined how to detect abnormal
behaviors for diverse people in a real-time and non-intrusive manner. Using video andii
relevant data sources (e.g., biometric and communication data) could be promising but
still need a baseline of normal behaviors for outlier detection.
This dissertation presents an anomaly detection framework that uses contextual
knowledge, contextual information, and contextual data for filtering visual information
extracted by computer vision techniques (ADCV) to address the challenges described
above. The framework categorizes the anomaly detection of civil infrastructures into two
categories: with and without a baseline of normal events. The author uses three case
studies to illustrate how the developed approaches can address ADCV challenges in
different categories of anomaly detection. Detailed data collection and experiments
validate the developed ADCV approaches.
ContributorsChen, Jiawei (Author) / Tang, Pingbo (Thesis advisor) / Ayer, Steven (Committee member) / Yang, Yezhou (Committee member) / Arizona State University (Publisher)
Created2020
Description
The Atlantic razor clam burrows underground with effectiveness and efficiency by coordinating shape changings of its shell and foot. Inspired by the burrowing strategy of razor clams, this research is dedicated to developing a self-burrowing technology for active underground explorations by investigating the burrowing mechanism of razor clams from the perspective of soil mechanics. In this study, the razor clam was observed to burrow out of sands simply by extending and contracting its foot periodically. This upward burrowing gait is much simpler than its downward burrowing gait, which also involves opening/closing of the shell and dilation of the foot. The upward burrowing gait inspired the design of a self-burrowing-out soft robot, which drives itself out of sands naturally by extension and contraction through pneumatic inflation and deflation. A simplified analytical model was then proposed and explained the upward burrowing behavior of the robot and razor clams as the asymmetric nature of soil resistances applied on both ends due to the intrinsic stress gradient of sand deposits. To burrow downward, additional symmetry-breaking features are needed for the robot to increase the resistance in the upward burrowing direction and to decrease the resistance in the downward burrowing direction. A potential approach is by incorporating friction anisotropy, which was then experimentally demonstrated to affect the upward burrowing of the soft robot. The downward burrowing gait of razor clams provides another inspiration. By exploring the analogies between the downward burrowing gait and in-situ soil characterization methods, a clam-inspired shape-changing penetrator was designed and penetrated dry granular materials both numerically and experimentally. Results demonstrated that the shell opening not only contributes to forming a penetration anchor by compressing the surrounding particles, but also reduces the foot penetration resistance temporally by creating a stress arch above the foot; the shell closing facilitates the downward burrowing by reducing the friction resistance to the subsequent shell retraction. Findings from this research shed lights on the future design of a clam-inspired self-burrowing robot.
ContributorsHuang, Sichuan (Author) / Tao, Junliang (Thesis advisor) / Kavazanjian, Edward (Committee member) / Marvi, Hamidreza (Committee member) / Zapata, Claudia (Committee member) / van Paassen, Leon (Committee member) / Arizona State University (Publisher)
Created2020
Description
Ultra High Performance (UHP) cementitious binders are a class of cement-based materials with high strength and ductility, designed for use in precast bridge connections, bridge superstructures, high load-bearing structural members like columns, and in structural repair and strengthening. This dissertation aims to elucidate the chemo-mechanical relationships in complex UHP binders to facilitate better microstructure-based design of these materials and develop machine learning (ML) models to predict their scale-relevant properties from microstructural information.To establish the connection between micromechanical properties and constitutive materials, nanoindentation and scanning electron microscopy experiments are performed on several cementitious pastes. Following Bayesian statistical clustering, mixed reaction products with scattered nanomechanical properties are observed, attributable to the low degree of reaction of the constituent particles, enhanced particle packing, and very low water-to-binder ratio of UHP binders. Relating the phase chemistry to the micromechanical properties, the chemical intensity ratios of Ca/Si and Al/Si are found to be important parameters influencing the incorporation of Al into the C-S-H gel.
ML algorithms for classification of cementitious phases are found to require only the intensities of Ca, Si, and Al as inputs to generate accurate predictions for more homogeneous cement pastes. When applied to more complex UHP systems, the overlapping chemical intensities in the three dominant phases – Ultra High Stiffness (UHS), unreacted cementitious replacements, and clinker – led to ML models misidentifying these three phases. Similarly, a reduced amount of data available on the hard and stiff UHS phases prevents accurate ML regression predictions of the microstructural phase stiffness using only chemical information. The use of generic virtual two-phase microstructures coupled with finite element analysis is also adopted to train MLs to predict composite mechanical properties. This approach applied to three different representations of composite materials produces accurate predictions, thus providing an avenue for image-based microstructural characterization of multi-phase composites such UHP binders. This thesis provides insights into the microstructure of the complex, heterogeneous UHP binders and the utilization of big-data methods such as ML to predict their properties. These results are expected to provide means for rational, first-principles design of UHP mixtures.
ML algorithms for classification of cementitious phases are found to require only the intensities of Ca, Si, and Al as inputs to generate accurate predictions for more homogeneous cement pastes. When applied to more complex UHP systems, the overlapping chemical intensities in the three dominant phases – Ultra High Stiffness (UHS), unreacted cementitious replacements, and clinker – led to ML models misidentifying these three phases. Similarly, a reduced amount of data available on the hard and stiff UHS phases prevents accurate ML regression predictions of the microstructural phase stiffness using only chemical information. The use of generic virtual two-phase microstructures coupled with finite element analysis is also adopted to train MLs to predict composite mechanical properties. This approach applied to three different representations of composite materials produces accurate predictions, thus providing an avenue for image-based microstructural characterization of multi-phase composites such UHP binders. This thesis provides insights into the microstructure of the complex, heterogeneous UHP binders and the utilization of big-data methods such as ML to predict their properties. These results are expected to provide means for rational, first-principles design of UHP mixtures.
ContributorsFord, Emily Lucile (Author) / Neithalath, Narayanan (Thesis advisor) / Rajan, Subramaniam D. (Committee member) / Mobasher, Barzin (Committee member) / Chawla, Nikhilesh (Committee member) / Hoover, Christian G. (Committee member) / Maneparambil, Kailas (Committee member) / Arizona State University (Publisher)
Created2020
Description
The recording of biosignals enables physicians to correctly diagnose diseases and prescribe treatment. Existing wireless systems failed to effectively replace the conventional wired methods due to their large sizes, high power consumption, and the need to replace batteries. This thesis aims to alleviate these issues by presenting a series of wireless fully-passive sensors for the acquisition of biosignals: including neuropotential, biopotential, intracranial pressure (ICP), in addition to a stimulator for the pacing of engineered cardiac cells. In contrast to existing wireless biosignal recording systems, the proposed wireless sensors do not contain batteries or high-power electronics such as amplifiers or digital circuitries. Instead, the RFID tag-like sensors utilize a unique radiofrequency (RF) backscattering mechanism to enable wireless and battery-free telemetry of biosignals with extremely low power consumption. This characteristic minimizes the risk of heat-induced tissue damage and avoids the need to use any transcranial/transcutaneous wires, and thus significantly enhances long-term safety and reliability. For neuropotential recording, a small (9mm x 8mm), biocompatible, and flexible wireless recorder is developed and verified by in vivo acquisition of two types of neural signals, the somatosensory evoked potential (SSEP) and interictal epileptic discharges (IEDs). For wireless multichannel neural recording, a novel time-multiplexed multichannel recording method based on an inductor-capacitor delay circuit is presented and tested, realizing simultaneous wireless recording from 11 channels in a completely passive manner. For biopotential recording, a wearable and flexible wireless sensor is developed, achieving real-time wireless acquisition of ECG, EMG, and EOG signals. For ICP monitoring, a very small (5mm x 4mm) wireless ICP sensor is designed and verified both in vitro through a benchtop setup and in vivo through real-time ICP recording in rats. Finally, for cardiac cell stimulation, a flexible wireless passive stimulator, capable of delivering stimulation current as high as 60 mA, is developed, demonstrating successful control over the contraction of engineered cardiac cells. The studies conducted in this thesis provide information and guidance for future translation of wireless fully-passive telemetry methods into actual clinical application, especially in the field of implantable and wearable electronics.
ContributorsLiu, Shiyi (Author) / Christen, Jennifer (Thesis advisor) / Nikkhah, Mehdi (Committee member) / Phillips, Stephen (Committee member) / Cao, Yu (Committee member) / Goryll, Michael (Committee member) / Arizona State University (Publisher)
Created2020
Description
The alternative project delivery methods (APDMs) today are being increasingly used by owner organizations in the architecture, engineering, and construction (AEC) industry. Yet the adoption of these methods can be extremely difficult to accomplish and requires significant change management efforts. To facilitate the APDM adoption, this research aimed to better understand how AEC owner organizations have changed from only using the design-bid-build method to also successfully implementing APDMs from an organizational change perspective. This research utilized a literature review, survey and interviews to fulfill the research objectives. The dissertation follows a three paper format. The first paper focuses on identifying organizational change management (OCM) practices that, when effectively executed, lead to increased success rates of adopting APDMs in owner AEC organizations. The results of the first paper indicated that the five OCM practices with the strongest correlations to successful APDM adoption were realistic timeframe, effective change agents, workload adjustments, senior-leadership commitment, and sufficient change-related training. The second paper focuses on investigating AEC employees’ reactions to the adoption of APDMs. The findings of the second paper revealed that employees in AEC organizations react favorably to adopting a change in their project delivery systems. The findings further revealed that increasing the use of OCM practices is related to decreased employee resistance to change. The third paper aimed to provide guidelines detailing on how to lead APDM adoption. The findings of the third paper indicated that there was a general sequence of four implementation phases, which were preparing and planning, pilot project testing, expanding to the intended scale, and sustaining and evaluating. The phases include specific OCM practices that increase the probability of successful APDM adoption. The dissertation results can help in guiding the senior managers of construction organizations and OCM consultants to effectively implement APDMs for the first time in the construction sector.
ContributorsAldossari, Khaled Medath (Author) / Sullivan, Kenneth T. (Thesis advisor) / Hurtado, Kristen C (Committee member) / Standage, Richard (Committee member) / Arizona State University (Publisher)
Created2020
Description
Cellular assays are the backbone of biological studies - be it for tissue modeling, drug discovery, therapeutics, or diagnostics. Two-dimensional (2D) cell culture has been deployed for several decades to garner physiologically relevant information and predict data before the cost-intensive animal testing. Although 2D techniques have been valuable for cellular assays, they have a colossal limitation - they do not adequately consider the natural three-dimensional (3D) microenvironment of the cells. As a result, they sometimes provide misleading statistics. Therefore, it is important to develop a 3D model that predicts cellular behaviors and their interaction with neighboring cells and extracellular matrix (ECM) in a more realistic manner. In recent biomedical research, various platforms have been modeled to generate 3D prototypes of tissues, spheroids, in vitro that could allow the study of cellular responses resembling in vivo environments, such as matrices, scaffolds, and devices. But most of these platforms have drawbacks such as lack of spheroid size control, low yield, or high cost associated with them. On the other hand, Amikagel is a low cost, high-fidelity platform that can facilitate the convenient generation of tumor and stem cell spheroids. Furthermore, Amikabeads are aminoglycoside-derived hydrogel microbeads derived from the same monomers as Amikagel. They are a versatile platform with several chemical groups that can be exploited for encapsulating the spheroids and investigating the delivery of bioactive compounds to the cells. This thesis is focused on engineering novel 3D tumor and stem cell models generated on Amikagel and encapsulated in Amikabeads for proximal delivery of bioactive compounds and applications in regenerative medicine.
ContributorsNanda, Tanya (Author) / Rege, Kaushal (Thesis advisor) / Blain Christen, Jennifer (Committee member) / Weaver, Jessica (Committee member) / Arizona State University (Publisher)
Created2020
Description
The construction industry generates tremendous amounts of data every day. Data can inform practitioners to increase their project performance as well as the quality of the resulting built environment. The data gathered from each stage has unique characteristics, and processing them to the appropriate information is critical. However, it is often difficult to measure the impact of the research across project phases (i.e., planning, design, construction, operation and maintenance, and end-of-life). The goal of this dissertation is to present how industry data can be used to make an impact on construction practices and test a suite of methods to measure the impact of construction research across project phases. The dissertation provides examples of impactful research studies for each project phase to demonstrate the collection and utilization of data generated from each stage and to assess the potential tangible impact on construction industry practices. The completed studies presented both quantitative and qualitative analyses. The first study focuses on the planning phase and provides a practice to improve frond end planning (FEP) implementation by developing the project definition rating index (PDRI) maturity and accuracy total rating system (MATRS). The second study uses earned value management system (EVMS) information from the design and construction phases to support reliable project control and management. The dissertation then provides a third study, this time focusing on the operations phase and comparing the impact of project delivery methods using the international roughness index (IRI). Lastly, the end-of-life or decommissioning phase is tackled through a study that gauges the monetary impact of the circular economy concept applied to reuse construction and demolition (C&D) waste. This dissertation measures the impact of the research according to the knowledge mobilization (KMb) theory, which illustrates the value of the work to the public and to practitioners.
ContributorsCho, Namho (Author) / El Asmar, Mounir (Thesis advisor) / Gibson, George (Committee member) / Kaloush, Kamil (Committee member) / Arizona State University (Publisher)
Created2020
Description
Travel time is the main transportation system performance measure used by the planning community to evaluate the impacts of traffic congestion on infrastructure investment projects and policy development plans. Planners rely on the travel demand model tool estimates for the selection and prioritization of critical and sensitive projects to meet the fiscally constraint requirements imposed by the Federal Highway Administration (FHWA) on their transportation improvement programs (TIP). While travel demand model estimates have been successfully implemented in the evaluation of project scenarios or alternatives, the application of the methods used in the travel demand model to generate these estimates continues to present a critical challenge, particularly to modelers who have to produce a validated model upon which traffic predictions can be made. The various volume-delay functions (VDFs) including the Bureau of Public Roads (BPR) function, used in the travel demand model to relate traffic volume to travel time, are developed based on system-wide attributes. BPR function in its polynomial form is computationally efficient and simple for implementation in a transport planning software. The planning community has long recognized that the BPR function cannot capture traffic flow dynamics and queue evolution processes. Besides, it has difficulties in using the average travel time measure to describe an oversaturated bottleneck with high density but low throughput. This dissertation aims to propose a simplified and yet effective point-queue based modeling approach built on the cumulative vehicle arrival concept, and the polynomial equation formula, based on Newell’s method, to estimate travel time at a corridor level using real-world speed and count measurements. A traffic state estimation (TSE) method is also proposed to characterize data into various states, such as congested state and uncongested state, using Markov Chain to capture current traffic pattern and Bayesian Classifier to infer congestion effects. As the testbed for the case study, the research selects the Phoenix freeway corridor with year-round traffic data collected from embedded traffic loop detectors. The results and effectiveness of the proposed methods are discussed to shed light on the calibration of link performance function, which is an analytical building block for system-wide performance evaluation.
ContributorsBelezamo, Baloka (Author) / Zhou, Xuesong (Thesis advisor) / Pendyala, Ram (Committee member) / Lou, Yingyan (Committee member) / Arizona State University (Publisher)
Created2020
Description
Stroke is a debilitating disorder and 75% of individuals with stroke (iwS) have upper extremity deficits. IwS are prescribed therapies to enhance upper-extremity mobility, but current most effective therapies have minimum requirements that the individuals with severe impairment do not meet. Thus, there is a need to enhance the therapies. Recent studies have shown that StartReact -the involuntary release of a planned movement, triggered by a startling stimulus (e.g., loud sound)- elicits faster and larger muscle activation in iwS compared to voluntary-initiated movement. However, StartReact has been only cursorily studied to date and there are some gaps in the StartReact knowledge. Previous studies have only evaluated StartReact on single-jointed movements in iwS, ignoring more functional tasks. IwS usually have abnormal flexor activity during extension tasks and abnormal muscle synergy especially during multi-jointed tasks; therefore, it is unknown 1) if more complex multi-jointed reach movements are susceptible to StartReact, and 2) if StartReact multi-jointed movements will be enhanced in the same way as single-jointed movements in iwS. In addition, previous studies showed that individuals with severe stroke, especially those with higher spasticity, experienced higher abnormal flexor muscle activation during StartReact trials. However, there is no study evaluating the impact of this elevated abnormal flexor activity on movement, muscle activation and muscle synergy alterations during voluntary-initiated movements after exposure to StartReact.
This dissertation evaluates StartReact and the voluntary trials before and after exposure to StartReact during a point-to-point multi-jointed reach task to three different targets covering a large workspace. The results show that multi-jointed reach tasks are susceptible to StartReact in iwS and the distance, muscle and movement onset speed, and muscle activations percentages and amplitude increase during StartReact trials. In addition, the distance, accuracy, muscle and movement onsets speeds, and muscle synergy similarity indices to the norm synergies increase during the voluntary-initiated trials after exposure to StartReact. Overall, this dissertation shows that exposure to StartReact did not impair voluntary-initiated movement and muscle synergy, but even improved them. Therefore, this study suggests that StartReact is safe for more investigations in training studies and therapy.
This dissertation evaluates StartReact and the voluntary trials before and after exposure to StartReact during a point-to-point multi-jointed reach task to three different targets covering a large workspace. The results show that multi-jointed reach tasks are susceptible to StartReact in iwS and the distance, muscle and movement onset speed, and muscle activations percentages and amplitude increase during StartReact trials. In addition, the distance, accuracy, muscle and movement onsets speeds, and muscle synergy similarity indices to the norm synergies increase during the voluntary-initiated trials after exposure to StartReact. Overall, this dissertation shows that exposure to StartReact did not impair voluntary-initiated movement and muscle synergy, but even improved them. Therefore, this study suggests that StartReact is safe for more investigations in training studies and therapy.
ContributorsRahimiTouranposhti, Marziye (Author) / Honeycutt, Claire F. (Thesis advisor) / Roh, Jinsook (Committee member) / Berman, Spring (Committee member) / Lee, Hyunglae (Committee member) / Marvi, Hamid (Committee member) / Schaefer, Sydney (Committee member) / Arizona State University (Publisher)
Created2020