Matching Items (48)
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- All Subjects: Microbiology
- Creators: School of Life Sciences
- Status: Published
Description
Long before “fake news” dominated the conversation within and about the media, media literacy advocates have championed the need for media literacy education that provides the tools for people to understand, analyze, and evaluate media messages. That the majority of U.S. adults now consume news on social media underscores the importance for students of all ages to be critical users of media. Furthermore, the affordances of social media to like, comment, and share news items within one’s network increases an individual’s responsibility to ascertain the veracity of news before using a social media megaphone to spread false information. Social media’s shareability can dictate how information spreads, increasing news consumers’ role as a gatekeeper of information and making media literacy education more important than ever.
This research examines the media literacy practices that news consumers use to inform their gatekeeping decisions. Using a constant comparative coding method, the author conducted a qualitative analysis of hundreds of discussion board posts from adult participants in a digital media literacy Massive Open Online Course (MOOC) to identify major themes and examine growth in participants’ sense of responsibility related to sharing news information, their feeling of empowerment to make informed decisions about the media messages they receive, and how the media literacy tools and techniques garnered from the MOOC have affected their daily media interactions. Findings emphasize the personal and contextual nature of media literacy, and that those factors must be addressed to ensure the success of a media literacy education program.
This research examines the media literacy practices that news consumers use to inform their gatekeeping decisions. Using a constant comparative coding method, the author conducted a qualitative analysis of hundreds of discussion board posts from adult participants in a digital media literacy Massive Open Online Course (MOOC) to identify major themes and examine growth in participants’ sense of responsibility related to sharing news information, their feeling of empowerment to make informed decisions about the media messages they receive, and how the media literacy tools and techniques garnered from the MOOC have affected their daily media interactions. Findings emphasize the personal and contextual nature of media literacy, and that those factors must be addressed to ensure the success of a media literacy education program.
ContributorsRoschke, Kristy (Author) / Thornton, Leslie-Jean (Thesis advisor) / Chadha, Monica (Committee member) / Halavais, Alexander (Committee member) / Silcock, Bill (Committee member) / Arizona State University (Publisher)
Created2018
Description
Space microbiology, or the study of microorganisms in space, has significant applications for both human spaceflight and Earth-based medicine. This thesis traces the evolution of the field of space microbiology since its creation in 1935. Beginning with simple studies to determine if terrestrial life could survive spaceflight, the field of space microbiology has grown to encompass a substantial body of work that is now recognized as an essential component of NASA' research endeavors. Part one provides an overview of the early period of space microbiology, from high-altitude balloon and rocket studies to work conducted during the Apollo program. Part two summarizes the current state of the field, with a specific focus on the revolutionary contributions made by the Nickerson lab at the Biodesign Institute at ASU using the NASA-designed Rotating Wall Vessel (RWV) Bioreactor. Finally, part three highlights the research I've conducted in the Nickerson lab, as well as continuing studies within the field of space microbiology.
ContributorsMcCarthy, Breanne E. (Author) / Lynch, John (Thesis director) / Foy, Joseph (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
Many bacteria actively import environmental DNA and incorporate it into their genomes. This behavior, referred to as transformation, has been described in many species from diverse taxonomic backgrounds. Transformation is expected to carry some selective advantages similar to those postulated for meiotic sex in eukaryotes. However, the accumulation of loss-of-function alleles at transformation loci and an increased mutational load from recombining with DNA from dead cells create additional costs to transformation. These costs have been shown to outweigh many of the benefits of recombination under a variety of likely parameters. We investigate an additional proposed benefit of sexual recombination, the Red Queen hypothesis, as it relates to bacterial transformation. Here we describe a computational model showing that host-pathogen coevolution may provide a large selective benefit to transformation and allow transforming cells to invade an environment dominated by otherwise equal non-transformers. Furthermore, we observe that host-pathogen dynamics cause the selection pressure on transformation to vary extensively in time, explaining the tight regulation and wide variety of rates observed in naturally competent bacteria. Host-pathogen dynamics may explain the evolution and maintenance of natural competence despite its associated costs.
ContributorsPalmer, Nathan David (Author) / Cartwright, Reed (Thesis director) / Wang, Xuan (Committee member) / Sievert, Chris (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
Clean water for drinking, food preparation, and bathing is essential for astronaut health and safety during long duration habitation of the International Space Station (ISS), including future missions to Mars. Despite stringent water treatment and recycling efforts on the ISS, it is impossible to completely prevent microbial contamination of onboard water supplies. In this work, we used a spaceflight analogue culture system to better understand how the microgravity environment can influence the pathogenesis-related characteristics of Burkholderia cepacia complex (Bcc), an opportunistic pathogen previously recovered from the ISS water system. The results of the present study suggest that there may be important differences in how this pathogen can respond and adapt to spaceflight and other low fluid shear environments encountered during their natural life cycles. Future studies are aimed at understanding the underlying mechanisms responsible for these phenotypes.
ContributorsKang, Bianca Younseon (Author) / Nickerson, Cheryl (Thesis director) / Barrila, Jennifer (Committee member) / Ott, Mark (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
Vaccinia virus (VV) is a prototype virus of the Orthopox viruses. The large dsDNA virus composed of 200kbp genome contains approximately 200 genes and replicates entirely in the cytosol. Since its use as a live vaccine against smallpox that leads to the successful eradication of smallpox, Vaccinia has been intensely studied as a vaccine vector since the large genome allows for the insertion of multiple genes. It is also studied as a molecular tool for gene therapy and gene functional study. Despite its success as a live vaccine, the vaccination causes some mild to serious bur rare adverse events in vaccinees such as generalized Vaccinia and encepharitis. Therefore, identification of virulence genes and removal of these genes to create a safer vaccine remain an important tasks. In this study, the author seeks to elucidate the possible relationship between immune evading proteins E3 and B19. VV did not allow double deletions of E3 and B19, indicating the existence of a relationship between the two genes.
ContributorsBarclay, Shizuka (Author) / Jacobs, Bertram (Thesis director) / Ugarova, Tatiana (Committee member) / Kibler, Karen (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
BACKGROUND: Biotechnology can improve vitamin deficiencies, farming practices and yields, yet it is surrounded by controversy. PURPOSE: The purpose of this study was to better understand opinions Americans have about genetically modified organisms (GMOs), across multiple perspectives including scientists, farmers, and perceptions shared via social media. METHODS: A Google Scholar search for the term "genetically modified" (GM) produced 1,420,000 results in 0.05 seconds from the year 1988 to present, a portion of this literature was used for this study. In addition a quasi-experimental study on social media (i.e. a blog and Twitter) was performed to inspire reactions of social media users who followed the accounts @Biofortified and @BiotechFood. The study lasted for approximately three months. The analytics website, Topsy was also used to track the number of conversations that included terms like "GMO". Furthermore a plant biologist, sustainability scientist, and local farmers were interviewed to gain insights on their perceptions of GM products. RESULTS: Results generally suggest that there was no stance shared by social media users, local farmers, and researchers. It was clear however that conversation about GMOs happens daily on social media. These conversations however lack the evidence that can be learned through literature and conversations with local farmers. DISCUSSION: A plausible possible reason for the confusion and mixed opinions is that regardless of the resources (like scientific literature and agriculture workers available on GMOs), individuals appear to use moral reasoning \u2014 as defined by Jonathan Haidt \u2014 to defend their stance on GMOs, not necessarily any empirical evidence.
ContributorsHubbard, Shayla Briann (Author) / Hekler, Eric (Thesis director) / Wharton, Christopher (Committee member) / Barrett, The Honors College (Contributor) / School of Community Resources and Development (Contributor) / School of Public Affairs (Contributor) / School of Life Sciences (Contributor)
Created2015-05
Description
This thesis explores how we can harness new technology to improve our relationship with companion animals and promote shelter animal welfare. The study looked into using the photo-sharing application Instagram to increase adoption rates at the Arizona Animal Welfare League & SPCA. An Instagram page was created and managed for the shelter, and data was collected regarding the impact the page had on adoption rates. The results were mixed, but overall it was determined that the Instagram page has unique value for the shelter.
ContributorsBautista-Hobin, Elena Maria (Author) / Minteer, Ben (Thesis director) / Ellison, Karin (Committee member) / Morefield, Michael (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor)
Created2015-05
Description
Although previous research has explored the relationship between social media use and well-being, many studies are contradictory of each other and conclude varying findings relating to social media use and outspokenness. This study explores the relationship between active and passive social media use, perceived social media expertise, and outspokenness using the potentially mediating variable of perceived social acceptance. 162 participants, recruited through Amazon Mechanical Turk (MTurk) and ASU’s SONA systems, completed a survey relating to their own use of social media, perceived social acceptance, and outspokenness. Contradictory to my first hypotheses, no significant correlations were found between social media use and social media expertise. However, correlation analyses revealed that active social media use is related to an increased amount of perceived social media expertise (r = 0.23, p < .004). Perceived social media expertise was significantly positively correlated with outspokenness (r = 0.19, p < 0.015); however, it was not correlated with perceived social acceptance. When examining these relationships separately by gender, a strong association was found for males between active social media use and outspokenness, whereas passive social media use and outspokenness were negatively correlated for females. The results of this study add to previous research in the field of social media and outspokenness and lend new ideas for future research on these topics, such as exploring the gender differences that are associated with these variables. Further research in the area is needed for a more complete understanding of how one’s social media use affects his/her outspokenness and how gender modifies these effects.
ContributorsRubino, Kelli Erika (Co-author) / Rubino, Kelli (Co-author) / Mickelson, Kristin (Thesis director) / Halavais, Alexander (Committee member) / Department of Psychology (Contributor) / School of International Letters and Cultures (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
This study examined the brand personality types and social media practices of six college athletic Twitter accounts. Specifically, this study investigated whether certain brand personalities corresponded with specific social media practices on Twitter. The author conducted a content analysis of each school's tweets to measure brand personality and scraped data in order to collect social media practice information. Results suggest that brand personality and social media practices are distinct. Extraversion was the most common personality type among all schools. In addition, schools that tweeted less frequently than others exhibited more brand personality and used more visual media.
ContributorsDave, Simran Sangita (Author) / Gilpin, Dawn (Thesis director) / Reed, Sada (Committee member) / Pucci, Jessica (Committee member) / School of Life Sciences (Contributor) / Walter Cronkite School of Journalism and Mass Communication (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
Renewable bioproduction through fermentation of microbial species such as E. coli shows much promise in comparison to conventional fossil fuel based chemical production. Although Escherichia coli is a workhorse for bioproduction, there are inherent limitations associated with the use of this organism which negatively affect bioproduction. One example is E. coli fermentative growth being less robust compared to some microbes such as Lactobacilli under anaerobic and microaerobic fermentation conditions. Identification and characterization of its fermentative growth constraints will help in making E. coli a better fermentation host. In this thesis, I demonstrate that Lactobacillus plantarum WCFS1 has desirable fermentative capabilities that may be transferrable to E. coli through genetic engineering to alleviate growth restraints. This has led to the hypothesis that these L. plantarum DNA sequences are transferrable through a genomic library. A background of comparative genomics and complementary literature review has demonstrated that E. coli growth may be hindered by stress from many toxin-antitoxin systems. L. plantarum WCFS1 optimizes amino acid catabolism over glycolysis to generate high ATP levels from reducing agents and proton motive force, and Lactobacilli are resistant to acidic environments and encodes a wide variety of acid transporters that could help E. coli fermentative growth. Since a great variety of L. plantarum genes may contribute to its fermentative capabilities, a gDNA library containing L. plantarum WCFS1 genes has been successfully constructed for testing in E. coli bioproducers to search for specific genes that may enhance E. coli fermentative performance and elucidate the molecular basis of Lactobacillus fermentative success.
ContributorsDufault, Matthew Elijah (Co-author, Co-author) / Wang, Xuan (Thesis director) / Nielsen, David (Committee member) / Varman, Arul (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05