Machine learning has a near infinite number of applications, of which the potential has yet to have been fully harnessed and realized. This thesis will outline two departments that machine learning can be utilized in, and demonstrate the execution of one methodology in each department. The first department that will be described is self-play in video games, where a neural model will be researched and described that will teach a computer to complete a level of Super Mario World (1990) on its own. The neural model in question was inspired by the academic paper “Evolving Neural Networks through Augmenting Topologies”, which was written by Kenneth O. Stanley and Risto Miikkulainen of University of Texas at Austin. The model that will actually be described is from YouTuber SethBling of the California Institute of Technology. The second department that will be described is cybersecurity, where an algorithm is described from the academic paper “Process Based Volatile Memory Forensics for Ransomware Detection”, written by Asad Arfeen, Muhammad Asim Khan, Obad Zafar, and Usama Ahsan. This algorithm utilizes Python and the Volatility framework to detect malicious software in an infected system.

Molecular pathology makes use of estimates of tumor content (tumor percentage) for pre-analytic and analytic purposes, such as molecular oncology testing, massive parallel sequencing, or next-generation sequencing (NGS), assessment of sample acceptability, accurate quantitation of variants, assessment of copy number changes (among other applications), determination of specimen viability for testing (since many assays require a minimum tumor content to report variants at the limit of detection) may all be improved with more accurate and reproducible estimates of tumor content. Currently, tumor percentages of samples submitted for molecular testing are estimated by visual examination of Hematoxylin and Eosin (H&E) stained tissue slides under the microscope by pathologists. These estimations can be automated, expedited, and rendered more accurate by applying machine learning methods on digital whole slide images (WSI).