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Forensic entomology is an important field of forensic science that utilizes insect evidence in criminal investigations. Blow flies (Diptera: Calliphoridae) are among the first colonizers of remains and are therefore frequently used in determining the minimum postmortem interval (mPMI). Blow fly development, however, is influenced by a variety of factors including temperature and feeding substrate type. Unfortunately, dietary fat content remains an understudied factor on the development process, which is problematic given the relatively high rates of obesity in the United States. To study the effects of fat content on blow fly development we investigated the survivorship, adult weight and development of Lucilia sericata (Meigen; Diptera: Calliphoridae) and Phormia regina (Meigen; Diptera: Calliphoridae) on ground beef with a 10%, 20%, or 27% fat content. As fat content increased, survivorship decreased across both species with P. regina being significantly impacted. While P. regina adults were generally larger than L. sericata across all fat levels, only L. sericata demonstrated a significant (P < 0.05) difference in weight by sex. Average total development times for P. regina are comparable to averages published in other literature. Average total development times for L. sericata, however, were nearly 50 hours higher. These findings provide insight on the effect of fat content on blow fly development, a factor that should be considered when estimating a mPMI. By understanding how fat levels affect the survivorship and development of the species studied here, we can begin improving the practice of insect evidence analysis in casework.
The subcategory of evidence deemed trace evidence is frequently seen in crime scenes, and while it is commonly the smallest evidence around, that doesn’t stop it from greatly contributing to the findings at the scene. Blood evidence may be categorized into this group in certain cases at crime scenes, especially in cases of transfer between two objects or people. In this study, the transfer of blood across both porous and non-porous substrates was examined to determine the persistence of blood across both substrates. The resulting stains after each trial of transfers were tested with a presumptive blood test commonly used in crime labs, the Kastle-Meyer test. Throughout all trials of the experiment, it was determined that blood on a non-porous surface typically dries faster as long as there isn’t a pooling effect, which hinders the ability for a stain to be continuously transferred and detected by Kastle-Meyer. Conversely, porous substrates are more likely to absorb and retain the blood in the material, allowing the blood to be released when pressure is applied, causing the stain to transfer more easily and result in a stain that will produce a positive Kastle-Meyer result.
Medicolegal forensic entomology is the study of insects to aid with legal investigations (Gemmellaro, 2017). Insect evidence can be used to provide information such as the post-mortem interval (PMI). Blow flies are especially useful as these insects are primary colonizers, quickly arriving at a corpse (Malainey & Anderson, 2020). The age of blow flies found at a scene is used to calculate the PMI. Blow fly age can be estimated using weather data as these insects are poikilothermic (Okpara, 2018). Morphological analysis also can be used to estimate age; however, it is more difficult with pupal samples as the pupae exterior does not change significantly as development progresses (Bala & Sharma, 2016). Gene regulation analysis can estimate the age of samples. MicroRNAs are short noncoding RNA that regulate gene expression (Cannell et al., 2008). Here, we aim to catalog miRNAs expressed during the development of three forensically relevant blow fly species preserved in several storage conditions. Results demonstrated that various miRNA sequences were differentially expressed across pupation. Expression of miR92b increased during mid pupation, aga-miR-92b expression increased during early pupation, and bantam, miR957, and dana-bantam-RA expression increased during late pupation. These results suggest that microRNA can be used to estimate the age of pupal samples as miRNA expression changes throughout pupation. Future work could develop a statistical model to accurately determine age using miRNA expression patterns.
In this experiment, the viability of gunshot residue (GSR) was examined. This was done through the very rarely researched intersection of forensic firearms analysis and forensic entomology. The question being resolved is if GSR can reliably be detected from secondary evidence transfer of GSR laden carrion onto flies and their larvae. While it is know that secondary and tertiary GSR evidence can be transferred by way of handshakes, no such research has been conducted on flies or their pupae. Findings indicated varying levels of detection of GSR on evidence. GSR could reliably be detected on fly bodies and their legs, but not on their pupae. This research is significant as it provides previously unknown information on this line of research and provides the groundwork for further research on this topic in the future.
Kantianism is a duty-based moral theory in which actions have an intrinsic moral worth. This means certain actions are morally right and other are morally wrong, regardless of the intended or realized consequences. The theory relies on the categorical imperative to judge the morality of certain actions. It states that an action is moral if its maxim can be willed universal law and if it avoids treating people as merely a means. In contrast, Utilitarianism is a consequentialist theory which focuses on the consequences of an action in judging moral worth. In Utilitarianism, the morally correct action is the one which will maximize utility; that is to say, the morally right action is the one which will produce the greatest amount of happiness and minimize the amount of pain for the greatest number of people.
After applying these two theories to moral dilemmas facing the U.S. Criminal Justice System, including the appropriate collection of DNA evidence, the use of police deception, and the use of criminal punishments such as solitary confinement or the death penalty, it was clear that Kantianism was the ethical theory best suited for guiding the system in treating people ethically. This is because Kantianism’s focus on the intrinsic moral worth of an action rather than its consequences leaves less room for ambiguity than does Utilitarianism.
Crime has become an inescapable part of being a consumer and viewer in today’s society. The interest in crime, however, could not have just been created by the media or a few specific parties and gained the amount of attention that is has today. Crime has been of interest since ancient times, seen immortalized in art and literature with famous events like the death of Socrates and the assassination of Julius Caesar. However, only more modern advances in media and the growth in consumerism could have led to interest and commodification of crime as we see it today. Landmark cases like Ted Bundy and O.J. Simpson have contributed to the growth of the media landscape, but because of the cyclical nature of the news and consumer cycle, these cases would not differ much in the way that they would be covered today, even with the new methods of disseminating information that we have today, such as social media and news websites that are accessible by everyone. Crime as entertainment has been a product not just of the media and popular culture but also from American consumers who continue to give media and pop culture producers a consumer who wishes to view their content and to continue to further the interest in crime. Media and popular culture are not the only people to blame for the popularization of crime as a product; people in the United States, and globally, are just as much responsible for the creation of crime as entertainment.
Even though writing this memoir is incredibly personal to myself, that does not discourage others from gaining something from reading this. I am not the first Asian American, ex-Mormon, college student or adopted individual who writes about their life and I most certainly will not be the last. If anyone is somewhat interested in any of the topics I am going to be writing about, then they can read this memoir and learn something. If not, then they can at least enjoy the stories and hopefully something I went through will put a smile on their face.
This research was conducted through the form of interview with Belizean citizens in Belize, Central America where I invited three of Belize’s most pivotal and influential figures behind social and civil injustices. Belize is a Caribbean country in Central America that was once a colony of the British known as “British Honduras”, gaining its independence on the 21st of September, 1981, making Belize the third to last youngest Caribbean country.
This has been made into a documentary that started filming back in September of 2017 during Belize’s 36th Independence Day where the country indulges in a month full of celebrations that brings a great feeling of togetherness for everyone. The film company that shot and edited this project is a local Belizean company by the name of KnightandDay Photography, with the consideration of helping to create work in Belize, support local business, and to be fully immersed in Belize and all of its resources.
This documentary is structured into five components: (1) Introduction; (2) Interview with guest number one; (3) Interview with guest number two; (4) Interview with guest number three; (5) Interview with five randomly selected Belizean citizens on the street; (6) Outro.
The main objective of this research was to speak in depth with specific Belizeans that have spent significant time in America, whether working, or going to school in order to have the knowledge to compare the experience of the black Belizean in their home country versus that of what America offers as far as the black experience and to explain the history of other ethnic groups of peoples that inhabits Belize and how the tensions and stereotypes among Belizeans arose over time.
