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Herpes simplex virus 2 (HSV-2) is one of the most common sexually transmitted infections (STI), affecting over 267 million women worldwide. HSV-2 causes a chronic, latent infection that increases the risk for acquisition with other STI, including HIV. Currently, there is no vaccine against HSV-2 and novel anti-viral treatments are

Herpes simplex virus 2 (HSV-2) is one of the most common sexually transmitted infections (STI), affecting over 267 million women worldwide. HSV-2 causes a chronic, latent infection that increases the risk for acquisition with other STI, including HIV. Currently, there is no vaccine against HSV-2 and novel anti-viral treatments are needed. IL-36γ is a newly characterized cytokine that has been shown to play a role in inflammation and be upregulated in response to microbial infection and tissue damage. We have shown that IL-36γ is expressed in the female reproductive tract (FRT) and is upregulated by HSV-2 infection in vitro and in vivo. IL-36γ in turn induces production of proinflammatory cytokines and chemokines in human vaginal epithelial cells (VEC) that can aid in immune cell recruitment. We hypothesize that IL-36γ is a key regulator of mucosal inflammation in the FRT and functions to limit HSV-2 infection. We have demonstrated that IL-36γ treatment prior to infection protects against HSV-2 replication, disease severity, and promotes survival in a lethal mouse model. Thus, the objective of this study is to understand the mechanisms whereby IL-36γ inhibits HSV-2 replication. To understand the impact of IL-36γ on the HSV-2 lifecycle, we pretreated VEC with IL-36γ and evaluated viral titer during virus attachment and entry, replication, and cell-to-cell spread by plaque assay. Pretreatment with IL-36γ 4h prior to infection did not significantly reduce viral titers in VEC monolayers relative to untreated groups. This suggesting that IL-36γ may play a more significant role in immune cell recruitment during HSV-2 infection. To test this, FRT tissue samples from HSV-2 infected IL-36γ -/- and WT mice were analyzed by histochemistry to characterize immune cell recruitment. No clear pattern was determined for tissue samples in which cell clusters were observed and cell type within recruited clusters was unable to be identified at the current magnification. As these projects continue, the data will aid in elucidating the mechanism and level to which IL-36γ impacts HSV-2 infection in human VEC and FRT models.
ContributorsAlexander, Thessaly E (Author) / Herbst-Kralovetz, Melissa (Thesis director) / Capco, David (Committee member) / Hogue, Ian (Committee member) / School of Human Evolution & Social Change (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description

For the past six months, I have been working with Black Lives Matter Phoenix Metro on the Tempe’s People’s Budget coalition. I served as a member on the research team as well as a temporary member of the Black Lives Matter’s nucleus leadership team. I joined weekly meetings for both

For the past six months, I have been working with Black Lives Matter Phoenix Metro on the Tempe’s People’s Budget coalition. I served as a member on the research team as well as a temporary member of the Black Lives Matter’s nucleus leadership team. I joined weekly meetings for both groups, and conducted research on city budget proposals, initiatives, and resources. I also lead discussions and conversations about progress, next steps, and goals of the coalition with over 50 volunteers within the coalition. The Tempe’s People Budget crafted a survey in late October and sent it out mid-November to members of the Tempe community, asking them what community resources they would use and which resources and investments they believed the city of Tempe should commit to. After five months of survey collection, we presented a preliminary budget proposal to Tempe’s financial office for consideration for the 2022-2023 budget, using data from 318 survey responses. With the creation of the survey among the adaption of the Tempe’s coalition, we wanted to look at what preventive community resource most respondents wanted Tempe to reinvest in. We found that the majority of survey respondents would like investments in renewable energy, housing stability, and alternatives to police. It is the city’s job to protect and serve all members of their communities, and public safety should be prioritized by investing in preventive measures instead of remedial punishments. Public safety concerns would be most effectively resolved by addressing issues such as: little to no income, housing instability, lack of access to food and water and other basic necessities to survive. Currently, Tempe is investing in the police, who most often only serve punishments.

ContributorsNelson, Phoenix (Author) / Cloutier, Scott (Thesis director) / Tekola, Sarra (Committee member) / Barrett, The Honors College (Contributor) / School of Human Evolution & Social Change (Contributor) / School of Politics and Global Studies (Contributor)
Created2022-05
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ContributorsNelson, Phoenix (Author) / Cloutier, Scott (Thesis director) / Tekola, Sarra (Committee member) / Barrett, The Honors College (Contributor) / School of Human Evolution & Social Change (Contributor)
Created2022-05
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ContributorsNelson, Phoenix (Author) / Cloutier, Scott (Thesis director) / Tekola, Sarra (Committee member) / Barrett, The Honors College (Contributor) / School of Human Evolution & Social Change (Contributor)
Created2022-05