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- All Subjects: Electrical Engineering
- All Subjects: Biology
- Creators: Arizona State University
- Status: Published
Description
Obesity impairs skeletal muscle maintenance and regeneration, a condition that can progressively lead to muscle loss, but the mechanisms behind it are unknown. Muscle is primarily composed of multinucleated cells called myotubes which are derived by the fusion of mononucleated myocytes. A key mediator in this process is the cellular fusion protein syncytin-1. This led to the hypothesis that syncytin-1 could be decreased in the muscle of obese/insulin resistant individuals. In contrast, it was found that obese/insulin resistant subjects had higher syncytin-1 expression in the muscle compared to that of the lean subjects. Across the subjects, syncytin-1 correlated significantly with body mass index, percent body fat, blood glucose and HbA1c levels, insulin sensitivity and muscle protein fractional synthesis rate. The concentrations of specific plasma fatty acids, such as the saturated fatty acid (palmitate) and monounsaturated fatty acid (oleate) are known to be altered in obese/insulin resistant humans, and also to influence the protein synthesis in muscle. Therefore, it was evaluated that the effects of palmitate and oleate on syncytin-1 expression, as well as 4E-BP1 phosphorylation, a key mechanism regulating muscle protein synthesis in insulin stimulated C2C12 myotubes. The results showed that treatment with 20 nM insulin, 300 µM oleate, 300 µM oleate +20 nM insulin and 300 µM palmitate + 300 µM oleate elevated 4E-BP1 phosphorylation. At the same time, 20 nM insulin, 300 µM palmitate, 300 µM oleate + 20 nM insulin and 300 µM palmitate + 300 µM oleate elevated syncytin-1 expression. Insulin stimulated muscle syncytin-1 expression and 4E-BP1 phosphorylation, and this effect was comparable to that observed in the presence of oleate alone. However, the presence of palmitate + oleate diminished the stimulatory effect of insulin on muscle syncytin-1 expression and 4E-BP1 phosphorylation. These findings indicate oleate but not palmitate increased total 4E-BP1 phosphorylation regardless of insulin and the presence of palmitate in insulin mediated C2C12 cells. The presence of palmitate inhibited the upregulation of total 4EB-P1 phosphorylation. Palmitate but not oleate increased syncytin-1 expression in insulin mediated C2C12 myotubes. It is possible that chronic hyperinsulinemia in obesity and/or elevated levels of fatty acids such as palmitate in plasma could have contributed to syncytin-1 overexpression and decreased muscle protein fractional synthesis rate in obese/insulin resistant human muscle.
ContributorsRavichandran, Jayachandran (Author) / Katsanos, Christos (Thesis advisor) / Coletta, Dawn (Committee member) / Dickinson, Jared (Committee member) / Arizona State University (Publisher)
Created2017
Description
High-efficiency DC-DC converters make up one of the important blocks of state-of-the-art power supplies. The trend toward high level of transistor integration has caused load current demands to grow significantly. Supplying high output current and minimizing output current ripple has been a driving force behind the evolution of Multi-phase topologies. Ability to supply large output current with improved efficiency, reduction in the size of filter components, improved transient response make multi-phase topologies a preferred choice for low voltage-high current applications.
Current sensing capability inside a system is much sought after for applications which include Peak-current mode control, Current limiting, Overload protection. Current sensing is extremely important for current sharing in Multi-phase topologies. Existing approaches such as Series resistor, SenseFET, inductor DCR based current sensing are simple but their drawbacks such low efficiency, low accuracy, limited bandwidth demand a novel current sensing scheme.
This research presents a systematic design procedure of a 5V - 1.8V, 8A 4-Phase Buck regulator with a novel current sensing scheme based on replication of the inductor current. The proposed solution consists of detailed system modeling in PLECS which includes modification of the peak current mode model to accommodate the new current sensing element, derivation of power-stage and Plant transfer functions, Controller design. The proposed model has been verified through PLECS simulations and compared with a transistor-level implementation of the system. The time-domain parameters such as overshoot and settling-time simulated through transistor-level
implementation is in close agreement with the results obtained from the PLECS model.
Current sensing capability inside a system is much sought after for applications which include Peak-current mode control, Current limiting, Overload protection. Current sensing is extremely important for current sharing in Multi-phase topologies. Existing approaches such as Series resistor, SenseFET, inductor DCR based current sensing are simple but their drawbacks such low efficiency, low accuracy, limited bandwidth demand a novel current sensing scheme.
This research presents a systematic design procedure of a 5V - 1.8V, 8A 4-Phase Buck regulator with a novel current sensing scheme based on replication of the inductor current. The proposed solution consists of detailed system modeling in PLECS which includes modification of the peak current mode model to accommodate the new current sensing element, derivation of power-stage and Plant transfer functions, Controller design. The proposed model has been verified through PLECS simulations and compared with a transistor-level implementation of the system. The time-domain parameters such as overshoot and settling-time simulated through transistor-level
implementation is in close agreement with the results obtained from the PLECS model.
ContributorsBurli, Venkatesh (Author) / Bakkaloglu, Bertan (Thesis advisor) / Garrity, Douglas (Committee member) / Kitchen, Jennifer (Committee member) / Arizona State University (Publisher)
Created2017
Description
In this dissertation, two problems are addressed in the verification and control of Cyber-Physical Systems (CPS):
1) Falsification: given a CPS, and a property of interest that the CPS must satisfy under all allowed operating conditions, does the CPS violate, i.e. falsify, the property?
2) Conformance testing: given a model of a CPS, and an implementation of that CPS on an embedded platform, how can we characterize the properties satisfied by the implementation, given the properties satisfied by the model?
Both problems arise in the context of Model-Based Design (MBD) of CPS: in MBD, the designers start from a set of formal requirements that the system-to-be-designed must satisfy.
A first model of the system is created.
Because it may not be possible to formally verify the CPS model against the requirements, falsification tries to verify whether the model satisfies the requirements by searching for behavior that violates them.
In the first part of this dissertation, I present improved methods for finding falsifying behaviors of CPS when properties are expressed in Metric Temporal Logic (MTL).
These methods leverage the notion of robust semantics of MTL formulae: if a falsifier exists, it is in the neighborhood of local minimizers of the robustness function.
The proposed algorithms compute descent directions of the robustness function in the space of initial conditions and input signals, and provably converge to local minima of the robustness function.
The initial model of the CPS is then iteratively refined by modeling previously ignored phenomena, adding more functionality, etc., with each refinement resulting in a new model.
Many of the refinements in the MBD process described above do not provide an a priori guaranteed relation between the successive models.
Thus, the second problem above arises: how to quantify the distance between two successive models M_n and M_{n+1}?
If M_n has been verified to satisfy the specification, can it be guaranteed that M_{n+1} also satisfies the same, or some closely related, specification?
This dissertation answers both questions for a general class of CPS, and properties expressed in MTL.
1) Falsification: given a CPS, and a property of interest that the CPS must satisfy under all allowed operating conditions, does the CPS violate, i.e. falsify, the property?
2) Conformance testing: given a model of a CPS, and an implementation of that CPS on an embedded platform, how can we characterize the properties satisfied by the implementation, given the properties satisfied by the model?
Both problems arise in the context of Model-Based Design (MBD) of CPS: in MBD, the designers start from a set of formal requirements that the system-to-be-designed must satisfy.
A first model of the system is created.
Because it may not be possible to formally verify the CPS model against the requirements, falsification tries to verify whether the model satisfies the requirements by searching for behavior that violates them.
In the first part of this dissertation, I present improved methods for finding falsifying behaviors of CPS when properties are expressed in Metric Temporal Logic (MTL).
These methods leverage the notion of robust semantics of MTL formulae: if a falsifier exists, it is in the neighborhood of local minimizers of the robustness function.
The proposed algorithms compute descent directions of the robustness function in the space of initial conditions and input signals, and provably converge to local minima of the robustness function.
The initial model of the CPS is then iteratively refined by modeling previously ignored phenomena, adding more functionality, etc., with each refinement resulting in a new model.
Many of the refinements in the MBD process described above do not provide an a priori guaranteed relation between the successive models.
Thus, the second problem above arises: how to quantify the distance between two successive models M_n and M_{n+1}?
If M_n has been verified to satisfy the specification, can it be guaranteed that M_{n+1} also satisfies the same, or some closely related, specification?
This dissertation answers both questions for a general class of CPS, and properties expressed in MTL.
ContributorsAbbas, Houssam Y (Author) / Fainekos, Georgios (Thesis advisor) / Duman, Tolga (Thesis advisor) / Mittelmann, Hans (Committee member) / Tsakalis, Konstantinos (Committee member) / Arizona State University (Publisher)
Created2015
Description
A new loop configuration capable of reducing power radiation magnitudes lower than conventional loops has been developed. This configuration is demonstrated for the case of two coaxial loops of 0.1 meter radius coupled via the magnetic reactive field. Utilizing electromagnetism theory, techniques from antenna design and a new near field design initiative, the ability to design a magnetic field has been investigated by using a full wave simulation tool. The method for realization is initiated from first order physics model, ADS and onto a full wave situation tool for the case of a non-radiating helical loop. The exploration into the design of a magnetic near field while mitigating radiation power is demonstrated using an real number of twists to form a helical wire loop while biasing the integer twisted loop in a non-conventional moebius termination. The helix loop setup as a moebius loop convention can also be expressed as a shorted antenna scheme. The 0.1 meter radius helix antenna is biased with a 1MHz frequency that categorized the antenna loop as electrically small. It is then demonstrated that helical configuration reduces the electric field and mitigates power radiation into the far field. In order to compare the radiated power reduction performance of the helical loop a shielded loop is used as a baseline for comparison. The shielded loop system of the same geometric size and frequency is shown to have power radiation expressed as -46.1 dBm. The power radiated mitigation method of the helix loop reduces the power radiated from the two loop system down to -98.72 dBm.
ContributorsMoreno, Fernando (Author) / Diaz, Rodolfo (Thesis advisor) / Aberle, James T., 1961- (Committee member) / Kozicki, Michael (Committee member) / Arizona State University (Publisher)
Created2015