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Description
The ability to edit chromosomal regions is an important tool for the study of gene function and the ability to engineer synthetic gene networks. CRISPR-Cas systems, a bacterial RNA-guided immune system against foreign nucleic acids, have recently been engineered for a plethora of genome engineering and transcriptional regulation applications. Here

The ability to edit chromosomal regions is an important tool for the study of gene function and the ability to engineer synthetic gene networks. CRISPR-Cas systems, a bacterial RNA-guided immune system against foreign nucleic acids, have recently been engineered for a plethora of genome engineering and transcriptional regulation applications. Here we employ engineered variants of CRISPR systems in proof-of-principle experiments demonstrating the ability of CRISPR-Cas derived single-DNA-strand cutting enzymes (nickases) to direct host-cell genomic recombination. E.coli is generally regarded as a poorly recombinogenic host with double-stranded DNA breaks being highly lethal. However, CRISPR-guided nickase systems can be easily programmed to make very precise, non-lethal, incisions in genomic regions directing both single reporter gene and larger-scale recombination events deleting up to 36 genes. Genome integrated repetitive elements of variable sizes can be employed as sites for CRISPR induced recombination. We project that single-stranded based editing methodologies can be employed alongside preexisting genome engineering techniques to assist and expedite metabolic engineering and minimalized genome research.
ContributorsStandage-Beier, Kylie S (Author) / Wang, Xiao (Thesis director) / Haynes, Karmella (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor)
Created2014-05
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Description
The goal of this study was to understand elementary school children’s perceptions of engineering. A total of 949 elementary school students were surveyed, individually or as a whole group, to examine gender and age differences in achievement-related beliefs (i.e., competency, interest, and importance) pertaining to engineering-related skills and activities. The

The goal of this study was to understand elementary school children’s perceptions of engineering. A total of 949 elementary school students were surveyed, individually or as a whole group, to examine gender and age differences in achievement-related beliefs (i.e., competency, interest, and importance) pertaining to engineering-related skills and activities. The results of this study found that specific skills and activities showed significant gender and age differences for each of the three measures. Significant findings showed that younger students (kindergarten through second grade) found many of the engineering-related skills and activities more interesting than the older students (third through fifth grade); however, the older students rated more of the skills and activities as being important. Gender differences showed that girls typically rated themselves as being more competent, more interested in, and valuing the skills and activities that pertained more to mindset ideas, such as learning from your mistakes and failures or not giving up, whereas boys rated themselves higher in more of the hands-on activities, such as building with things like legos, blocks, and k’nex.
ContributorsHandlos, Jamie Lynn Harte (Author) / Miller, Cindy (Thesis director) / Reisslein, Martin (Committee member) / School of Life Sciences (Contributor) / Chemical Engineering Program (Contributor, Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
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Description

Time studies are an effective tool to analyze current production systems and propose improvements. The problem that motivated the project was that conducting time studies and observing the progression of components across the factory floor is a manual process. Four Industrial Engineering students worked with a manufacturing company to develo

Time studies are an effective tool to analyze current production systems and propose improvements. The problem that motivated the project was that conducting time studies and observing the progression of components across the factory floor is a manual process. Four Industrial Engineering students worked with a manufacturing company to develop Computer Vision technology that would automate the data collection process for time studies. The team worked in an Agile environment to complete over 120 classification sets, create 8 strategy documents, and utilize Root Cause Analysis techniques to audit and validate the performance of the trained Computer Vision data models. In the future, there is an opportunity to continue developing this product and expand the team’s work scope to apply more engineering skills on the data collected to drive factory improvements.

Contributorsde Guzman, Lorenzo (Co-author) / Chmelnik, Nathan (Co-author) / Martz, Emma (Co-author) / Johnson, Katelyn (Co-author) / Ju, Feng (Thesis director) / Courter, Brandon (Committee member) / Industrial, Systems & Operations Engineering Prgm (Contributor, Contributor) / School of Politics and Global Studies (Contributor) / Industrial, Systems & Operations Engineering Prgm (Contributor, Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
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Description

Time studies are an effective tool to analyze current production systems and propose improvements. The problem that motivated the project was that conducting time studies and observing the progression of components across the factory floor is a manual process. Four Industrial Engineering students worked with a manufacturing company to develo

Time studies are an effective tool to analyze current production systems and propose improvements. The problem that motivated the project was that conducting time studies and observing the progression of components across the factory floor is a manual process. Four Industrial Engineering students worked with a manufacturing company to develop Computer Vision technology that would automate the data collection process for time studies. The team worked in an Agile environment to complete over 120 classification sets, create 8 strategy documents, and utilize Root Cause Analysis techniques to audit and validate the performance of the trained Computer Vision data models. In the future, there is an opportunity to continue developing this product and expand the team’s work scope to apply more engineering skills on the data collected to drive factory improvements.

ContributorsJohnson, Katelyn Rose (Co-author) / Martz, Emma (Co-author) / Chmelnik, Nathan (Co-author) / de Guzman, Lorenzo (Co-author) / Ju, Feng (Thesis director) / Courter, Brandon (Committee member) / Industrial, Systems & Operations Engineering Prgm (Contributor, Contributor) / Industrial, Systems & Operations Engineering Prgm (Contributor, Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
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Description

Time studies are an effective tool to analyze current production systems and propose improvements. The problem that motivated the project was that conducting time studies and observing the progression of components across the factory floor is a manual process. Four Industrial Engineering students worked with a manufacturing company to develo

Time studies are an effective tool to analyze current production systems and propose improvements. The problem that motivated the project was that conducting time studies and observing the progression of components across the factory floor is a manual process. Four Industrial Engineering students worked with a manufacturing company to develop Computer Vision technology that would automate the data collection process for time studies. The team worked in an Agile environment to complete over 120 classification sets, create 8 strategy documents, and utilize Root Cause Analysis techniques to audit and validate the performance of the trained Computer Vision data models. In the future, there is an opportunity to continue developing this product and expand the team’s work scope to apply more engineering skills on the data collected to drive factory improvements.

ContributorsChmelnik, Nathan (Co-author) / de Guzman, Lorenzo (Co-author) / Johnson, Katelyn (Co-author) / Martz, Emma (Co-author) / Ju, Feng (Thesis director) / Courter, Brandon (Committee member) / Industrial, Systems & Operations Engineering Prgm (Contributor, Contributor) / Industrial, Systems & Operations Engineering Prgm (Contributor, Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
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Description

Time studies are an effective tool to analyze current production systems and propose improvements. The problem that motivated the project was that conducting time studies and observing the progression of components across the factory floor is a manual process. Four Industrial Engineering students worked with a manufacturing company to develo

Time studies are an effective tool to analyze current production systems and propose improvements. The problem that motivated the project was that conducting time studies and observing the progression of components across the factory floor is a manual process. Four Industrial Engineering students worked with a manufacturing company to develop Computer Vision technology that would automate the data collection process for time studies. The team worked in an Agile environment to complete over 120 classification sets, create 8 strategy documents, and utilize Root Cause Analysis techniques to audit and validate the performance of the trained Computer Vision data models. In the future, there is an opportunity to continue developing this product and expand the team’s work scope to apply more engineering skills on the data collected to drive factory improvements.

ContributorsMartz, Emma Marie (Co-author) / de Guzman, Lorenzo (Co-author) / Johnson, Katelyn (Co-author) / Chmelnik, Nathan (Co-author) / Ju, Feng (Thesis director) / Courter, Brandon (Committee member) / Industrial, Systems & Operations Engineering Prgm (Contributor, Contributor) / Industrial, Systems & Operations Engineering Prgm (Contributor, Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
Description

Previous recombination rate estimation studies in rhesus macaques have been mostly restricted to a singular approach (e.g., using microsatellite loci). Here, we employ a bilateral method in estimating recombination rates—pedigree-based and linkage-disequilibrium-based—from whole-genome data of rhesus macaques to estimate CO and NCO recombination events and to compare contemporary and historical

Previous recombination rate estimation studies in rhesus macaques have been mostly restricted to a singular approach (e.g., using microsatellite loci). Here, we employ a bilateral method in estimating recombination rates—pedigree-based and linkage-disequilibrium-based—from whole-genome data of rhesus macaques to estimate CO and NCO recombination events and to compare contemporary and historical rates of recombination.

ContributorsWeiss, Sarah (Author) / Pfeifer, Susanne (Thesis director) / Versoza, Cyril (Committee member) / Barrett, The Honors College (Contributor) / School of Art (Contributor) / School of Life Sciences (Contributor)
Created2023-05
ContributorsWeiss, Sarah (Author) / Pfeifer, Susanne (Thesis director) / Versoza, Cyril (Committee member) / Barrett, The Honors College (Contributor) / School of Art (Contributor) / School of Life Sciences (Contributor)
Created2023-05
ContributorsWeiss, Sarah (Author) / Pfeifer, Susanne (Thesis director) / Versoza, Cyril (Committee member) / Barrett, The Honors College (Contributor) / School of Art (Contributor) / School of Life Sciences (Contributor)
Created2023-05
ContributorsWeiss, Sarah (Author) / Pfeifer, Susanne (Thesis director) / Versoza, Cyril (Committee member) / Barrett, The Honors College (Contributor) / School of Art (Contributor) / School of Life Sciences (Contributor)
Created2023-05