Protein and gene circuit level synthetic bioengineering can require years to develop a single target. Phage assisted continuous evolution (PACE) is a powerful new tool for rapidly engineering new genes and proteins, but the method requires an automated cell culture system, making it inaccessible to non industrial research programs. Complex protein functions, like specific binding, require similarly dynamic PACE selection that can be alternatively induced or suppressed, with heat labile chemicals like tetracycline. Selection conditions must be controlled continuously over days, with adjustments made every few minutes. To make PACE experiments accessible to the broader community, we designed dedicated cell culture hardware and integrated optogenetically controlled plasmids. The low cost and open source platform allows a user to conduct PACE with continuous monitoring and precise control of evolution using light.

Motorcycles must be designed for safety and long operation. Front suspension systems must in turn be safe and able to operate for long service lives. Challenges to achieving safe and long service lifetimes include designing components (rims, axles, forks, etc.) to withstand various loading conditions not just once but numerous times as a matter of fatigue life. An already developed CAD model of a motorcycle suspension was taken and optimized for various loading conditions. These conditions included static loading, braking, cornering, and wheelie and front impact loads. In all cases, front impact load was the critical loading condition when FEA in SolidWorks Simulation was conducted for the components. All components were then optimized to handle the impact load by changing geometry until safety factors of 4.0 ± 0.25 were achieved. Components were then analyzed for fatigue life, with all steel and magnesium components having infinite predicted fatigue lives and all aluminum components having fatigue lives predicted with corrected S-N curves created for up to 500 million loading cycles. The design was optimized with all components becoming improved for stress compliance, with room for improvement existing in both defining loads for analysis and developing more accurate and rigorous fatigue life models.

The objective of this thesis is to conduct a case study into the Bell X-2, an early supersonic research aircraft utilizing a modern perspective and computational tools. The Bell X-2 was the second in a series of supersonic research aircraft created by Bell Aviation Corporation, designed to help engineers to explore this new region of flight. The goal of the X-2 was to gather data on high Mach Number and high-altitude flight as well as aerodynamic heating. The X-2 had poor lateral stability resulting in it being unstable at high Mach Numbers and moderate angles of attack. The program was full of new and unforeseen technical challenges resulting in many delays and tragedies. The program ended when stability problems resulted in a fatal crash destroying the aircraft and killing the test pilot. This case study addresses the historical background of the program, human influence, the stability problems encountered and conducting a stability analysis of the aircraft. To conduct the stability analysis, the potential flow solver, VORLAX, was used to gather aerodynamic coefficient data of the X-2 and determine if these stability problems could be determined from the data obtained. By comparing the results from VORLAX to a wind tunnel study, I determined that the poor lateral directional stability and control coupling issues were foreseeable in the initial design.

The purpose of this project was to develop a system capable of launching projectiles at a curved trajectory. This system effectively imparts spin on projectiles, enabling controlled indirect fire for the intended use of military operations. Through this proof of concept, it was determined whether a scaled system would be a viable solution to the issue of controlled indirect fire in dense urban areas. Using a series of coaxial motors with independently controlled speeds, it was possible to alter the horizontal and vertical displacement of objects in flight.