Protein and gene circuit level synthetic bioengineering can require years to develop a single target. Phage assisted continuous evolution (PACE) is a powerful new tool for rapidly engineering new genes and proteins, but the method requires an automated cell culture system, making it inaccessible to non industrial research programs. Complex protein functions, like specific binding, require similarly dynamic PACE selection that can be alternatively induced or suppressed, with heat labile chemicals like tetracycline. Selection conditions must be controlled continuously over days, with adjustments made every few minutes. To make PACE experiments accessible to the broader community, we designed dedicated cell culture hardware and integrated optogenetically controlled plasmids. The low cost and open source platform allows a user to conduct PACE with continuous monitoring and precise control of evolution using light.

As a result of the increase of pollution related to industrialization in Vietnam, acid rain has become a prevalent issue for Vietnamese farmers who are forced to rinse their crops – risking damage due to overwatering and poor harvest. Thus, the team was motivated to develop a solution to harmful impacts of acidic rainwater by creating a system with the ability to capture rainwater and determine its level of acidity in order to optimize the crop watering process, and promote productive crops. By conducting preliminary research on rainfall and tropical climate in Vietnam, existing products on the market, and pH sensors for monitoring and device material, the team was able to design a number of devices to collect, store, and measure the pH of rainwater. After developing a number of initial design requirements based on the needs of the farmers, a final prototype was developed using the best aspects of each initial design. Tests were conducted with varying structural and aqueous materials to represent a broad range of environmental conditions. While the scope of the project was ultimately limited to prototyping purposes, the principles explored throughout this thesis project can successfully be applied to a fully-functioning production model available for commercial use on Vietnamese farms. Given more time for development, improvements would be made in the extent of materials tested, and the configuration of electronics and data acquisition, in order to further optimize the process of determining rainwater acidity.

As a result of the increase of pollution related to industrialization in Vietnam, acid rain has become a prevalent issue for Vietnamese farmers who are forced to rinse their crops – risking damage due to overwatering and poor harvest. Thus, the team was motivated to develop a solution to harmful impacts of acidic rainwater by creating a system with the ability to capture rainwater and determine its level of acidity in order to optimize the crop watering process, and promote productive crops. By conducting preliminary research on rainfall and tropical climate in Vietnam, existing products on the market, and pH sensors for monitoring and device material, the team was able to design a number of devices to collect, store, and measure the pH of rainwater. After developing a number of initial design requirements based on the needs of the farmers, a final prototype was developed using the best aspects of each initial design. Tests were conducted with varying structural and aqueous materials to represent a broad range of environmental conditions. While the scope of the project was ultimately limited to prototyping purposes, the principles explored throughout this thesis project can successfully be applied to a fully-functioning production model available for commercial use on Vietnamese farms. Given more time for development, improvements would be made in the extent of materials tested, and the configuration of electronics and data acquisition, in order to further optimize the process of determining rainwater acidity.

As a result of the increase of pollution related to industrialization in Vietnam, acid rain has become a prevalent issue for Vietnamese farmers who are forced to rinse their crops – risking damage due to overwatering and poor harvest. Thus, the team was motivated to develop a solution to harmful impacts of acidic rainwater by creating a system with the ability to capture rainwater and determine its level of acidity in order to optimize the crop watering process, and promote productive crops. By conducting preliminary research on rainfall and tropical climate in Vietnam, existing products on the market, and pH sensors for monitoring and device material, the team was able to design a number of devices to collect, store, and measure the pH of rainwater. After developing a number of initial design requirements based on the needs of the farmers, a final prototype was developed using the best aspects of each initial design. Tests were conducted with varying structural and aqueous materials to represent a broad range of environmental conditions. While the scope of the project was ultimately limited to prototyping purposes, the principles explored throughout this thesis project can successfully be applied to a fully-functioning production model available for commercial use on Vietnamese farms. Given more time for development, improvements would be made in the extent of materials tested, and the configuration of electronics and data acquisition, in order to further optimize the process of determining rainwater acidity.