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This study was conducted as part of an underlying initiative to elucidate the mechanism of action of natural antibacterial clay minerals for application as therapeutic agents for difficult-to-treat infections such as methicillin-resistant Staphylococcus aureus (MRSA)-derived skin lesions and Buruli ulcer. The goal of this investigation was to determine whether exposure

This study was conducted as part of an underlying initiative to elucidate the mechanism of action of natural antibacterial clay minerals for application as therapeutic agents for difficult-to-treat infections such as methicillin-resistant Staphylococcus aureus (MRSA)-derived skin lesions and Buruli ulcer. The goal of this investigation was to determine whether exposure to the leachate of an antibacterial clay mineral, designated as CB, produced DNA double-strand breaks (DSBs) in Escherichia coli. A neutral comet assay for bacterial cells was adapted to assess DSB levels upon exposure to soluble antimicrobial compounds. Challenges involved with the adaptation process included comet visualization and data collection. To appropriately account for antimicrobial-mediated strand fragmentation, suitable control reactions comprised of exposures to water, ethanol, kanamycin, and bleomycin were developed and optimized for the assay. Bacterial exposure to CB resulted in significantly longer comet lengths compared to negative control exposures, suggesting that CB killing activity involves the induction of DNA DSBs. The results of this investigation further characterize the antimicrobial mechanisms associated with a particular clay mineral mixture. The adapted comet assay protocol described herein functions as an effective tool to assess double-strand fragmentation resulting from exposure to soluble antimicrobial compounds and to visually compare results from experimental and control reactions.
ContributorsSolanky, Dipesh (Author) / Haydel, Shelley (Thesis director) / Stout, Valerie (Committee member) / Adusumilli, Sarojini (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor)
Created2012-12
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Description
Glioblastoma multiforme (GBMs) is the most prevalent brain tumor type and causes approximately 40% of all non-metastic primary tumors in adult patients [1]. GBMs are malignant, grade-4 brain tumors, the most aggressive classication as established by the World Health Organization and are marked by their low survival rate; the median

Glioblastoma multiforme (GBMs) is the most prevalent brain tumor type and causes approximately 40% of all non-metastic primary tumors in adult patients [1]. GBMs are malignant, grade-4 brain tumors, the most aggressive classication as established by the World Health Organization and are marked by their low survival rate; the median survival time is only twelve months from initial diagnosis: Patients who live more than three years are considered long-term survivors [2]. GBMs are highly invasive and their diffusive growth pattern makes it impossible to remove the tumors by surgery alone [3]. The purpose of this paper is to use individual patient data to parameterize a model of GBMs that allows for data on tumor growth and development to be captured on a clinically relevant time scale. Such an endeavor is the rst step to a clinically applicable predictions of GBMs. Previous research has yielded models that adequately represent the development of GBMs, but they have not attempted to follow specic patient cases through the entire tumor process. Using the model utilized by Kostelich et al. [4], I will attempt to redress this deciency. In doing so, I will improve upon a family of models that can be used to approximate the time of development and/or structure evolution in GBMs. The eventual goal is to incorporate Magnetic Resonance Imaging (MRI) data into a parameterized model of GBMs in such a way that it can be used clinically to predict tumor growth and behavior. Furthermore, I hope to come to a denitive conclusion as to the accuracy of the Koteslich et al. model throughout the development of GBMs tumors.
ContributorsManning, Miles (Author) / Kostelich, Eric (Thesis director) / Kuang, Yang (Committee member) / Preul, Mark (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor)
Created2012-12
Description

Medical recovery time continues to be a drawback for many medical diagnoses and procedures. Prolonged recovery affects all aspects of the population, and targets different avenues of everyday life. Avenues such as providing, attending a job, personal objectives in different ways and even their own well-being. To combat this one

Medical recovery time continues to be a drawback for many medical diagnoses and procedures. Prolonged recovery affects all aspects of the population, and targets different avenues of everyday life. Avenues such as providing, attending a job, personal objectives in different ways and even their own well-being. To combat this one area of research that has gained tremendous awareness in recent years is that of platelet-rich fibrin (PRF), which has been utilized across a wide variety of medical fields for the regeneration of soft tissues. PRF, or platelet-rich fibrin, is the next generation treatment of platelet concentrate. PRF is a fibrin matrix composed of platelet cytokines, growth factors and cells used to help wound healing and tissue regeneration. The objective of this thesis is to investigate the potential recovery time difference with PRF incorporation for common medical procedures. The experimental group included three individuals who had PRF treatment at any point during any sort of medical operation. The control group included individuals who did not have PRF treatment at any point and also those who had no prior knowledge of this method of treatment. Results were mixed because of the variative behind the medical procedures. Through observation, PRF treatment improved tolerance of pain, well-being of patients and quality of recovery with three different domains of inquiry per patient testimony. This case-analysis of 6 patients is a preliminary study and therefore inconclusive. PRF is a promising approach and this study suggests that it could potentially be a new medical approach to treatment.

ContributorsBuch, Ajay (Author) / Kingsbury, Jeffrey (Thesis director) / Gaesser, Glenn (Committee member) / Barrett, The Honors College (Contributor) / College of Health Solutions (Contributor)
Created2023-05
ContributorsMichels, Bailey (Author) / O'Flaherty, Katherine (Thesis director) / Rasmussen, Elizabeth (Committee member) / Barrett, The Honors College (Contributor) / College of Health Solutions (Contributor)
Created2023-05
ContributorsMichels, Bailey (Author) / O'Flaherty, Katherine (Thesis director) / Rasmussen, Elizabeth (Committee member) / Barrett, The Honors College (Contributor) / College of Health Solutions (Contributor)
Created2023-05
Description

This research paper focuses on how the idea of suffering has evolved over time in the United States healthcare system. Different aspects like long vs short-term illnesses, bias, and more were inspected to determine how they play a part in increased or decreased patient suffering. The final determination of how

This research paper focuses on how the idea of suffering has evolved over time in the United States healthcare system. Different aspects like long vs short-term illnesses, bias, and more were inspected to determine how they play a part in increased or decreased patient suffering. The final determination of how suffering in the system has evolved and what to do with this information is also discussed.

ContributorsMichels, Bailey (Author) / O'Flaherty, Katherine (Thesis director) / Rasmussen, Elizabeth (Committee member) / Barrett, The Honors College (Contributor) / College of Health Solutions (Contributor)
Created2023-05
Description

This thesis explores the ethical implications of using facial recognition artificial intelligence (AI) technologies in medicine, with a focus on both the opportunities and challenges presented by the use of this technology in the diagnosis and treatment of rare genetic disorders. We highlight the positive outcomes of using AI in

This thesis explores the ethical implications of using facial recognition artificial intelligence (AI) technologies in medicine, with a focus on both the opportunities and challenges presented by the use of this technology in the diagnosis and treatment of rare genetic disorders. We highlight the positive outcomes of using AI in medicine, such as accuracy and efficiency in diagnosing rare genetic disorders, while also examining the ethical concerns including bias, misdiagnosis, the issues it may cause within patient-clinician relationships, misuses outside of medicine, and privacy. This paper draws on the opinions of medical providers and other professionals outside of medicine, which finds that while many are excited about the potential of AI to improve medicine, concerns remain about the ethical implications of these technologies. We discuss current legislation controlling the use of AI in healthcare and its ambiguity. Overall, this thesis highlights the need for further research and public discourse to address the ethical implications of using facial recognition and AI technologies in medicine, while also providing recommendations for its future use in medicine.

ContributorsVargas Jordan, Anna (Author) / Kohlenberg, Maiya (Co-author) / Martin, Thomas (Thesis director) / Sellner, Erin (Committee member) / Barrett, The Honors College (Contributor) / College of Health Solutions (Contributor)
Created2023-05
Description
This paper explores the benefits reading and writing medical poetry can benefit preprofessional/medical students, physicians, and patients as a means to share the experiences they
encounter in the medical world. The concept for this paper originates from the idea of narrative
medicine as a way to foster relationships between physicians and patients

This paper explores the benefits reading and writing medical poetry can benefit preprofessional/medical students, physicians, and patients as a means to share the experiences they
encounter in the medical world. The concept for this paper originates from the idea of narrative
medicine as a way to foster relationships between physicians and patients through the sharing of
stories, or narratives, between the two parties. In efforts to help teach this skill, universities and
medical schools have begun to offer courses in the medical humanities. The goal of these courses
is to teach students how to develop the skills they need to empathize and learn from their
patients’ experiences. Paired with the traditional rigor of a science-based curriculum, the medical
humanities have become part of medical schools’ efforts to “train the whole physician.”
Medical poetry is an example of the types of humanities courses that can benefit students
interested in medicine. The history of medical poetry spans across decades of literary history.
Beginning with the early references of medicine from the ancient world to the contemporary
work of the present, poets of different backgrounds and histories are discussed. Research to
support the efficacy of medical poetry include studies done on how medical poetry has impacted
students, readers, and patients. Finally, the author’s experiences as both a pre-professional
student and patient are shared to further explore the benefits that reading, and writing can bring.
ContributorsVilla, Rosario Alicia (Author) / Dombroski, Rosemarie (Thesis director) / Hanlon, Christopher (Committee member) / College of Health Solutions (Contributor) / Department of English (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05