Matching Items (4)
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- All Subjects: medicine
- All Subjects: Spanish
- Creators: College of Liberal Arts and Sciences
Description
This study was conducted as part of an underlying initiative to elucidate the mechanism of action of natural antibacterial clay minerals for application as therapeutic agents for difficult-to-treat infections such as methicillin-resistant Staphylococcus aureus (MRSA)-derived skin lesions and Buruli ulcer. The goal of this investigation was to determine whether exposure to the leachate of an antibacterial clay mineral, designated as CB, produced DNA double-strand breaks (DSBs) in Escherichia coli. A neutral comet assay for bacterial cells was adapted to assess DSB levels upon exposure to soluble antimicrobial compounds. Challenges involved with the adaptation process included comet visualization and data collection. To appropriately account for antimicrobial-mediated strand fragmentation, suitable control reactions comprised of exposures to water, ethanol, kanamycin, and bleomycin were developed and optimized for the assay. Bacterial exposure to CB resulted in significantly longer comet lengths compared to negative control exposures, suggesting that CB killing activity involves the induction of DNA DSBs. The results of this investigation further characterize the antimicrobial mechanisms associated with a particular clay mineral mixture. The adapted comet assay protocol described herein functions as an effective tool to assess double-strand fragmentation resulting from exposure to soluble antimicrobial compounds and to visually compare results from experimental and control reactions.
ContributorsSolanky, Dipesh (Author) / Haydel, Shelley (Thesis director) / Stout, Valerie (Committee member) / Adusumilli, Sarojini (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor)
Created2012-12
Description
This project aims to provide a contextualized history of the Sky Harbor Neighborhood Association‟s community collective action efforts. The Sky Harbor Neighborhood (SHN) of East Phoenix is bounded on the West by 24th St., on the East by 32nd St., on the North by Roosevelt St., and the South by Washington Street. SHN is a majority Latino, low-income, working class community (U.S. Census Bureau, 2010) that faces a variety of challenges including low walkability due to inadequate pedestrian infrastructure, low tree coverage, and crime. East Van Buren St., which has a reputation for being one of Phoenix‟s red-light districts, splits the neighborhood in two. In addition, the SHN lacks some key amenities such as grocery stores and is partly considered a food desert by the United States Department of Agriculture (USDA Economic Research Service, 2012).
ContributorsPearson, Kimberly (Author) / Golub, Aaron (Thesis director) / Wiek, Arnim (Committee member) / York, Abigail (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor) / School of Sustainability (Contributor)
Created2012-12
Description
Glioblastoma multiforme (GBMs) is the most prevalent brain tumor type and causes approximately 40% of all non-metastic primary tumors in adult patients [1]. GBMs are malignant, grade-4 brain tumors, the most aggressive classication as established by the World Health Organization and are marked by their low survival rate; the median survival time is only twelve months from initial diagnosis: Patients who live more than three years are considered long-term survivors [2]. GBMs are highly invasive and their diffusive growth pattern makes it impossible to remove the tumors by surgery alone [3]. The purpose of this paper is to use individual patient data to parameterize a model of GBMs that allows for data on tumor growth and development to be captured on a clinically relevant time scale. Such an endeavor is the rst step to a clinically applicable predictions of GBMs. Previous research has yielded models that adequately represent the development of GBMs, but they have not attempted to follow specic patient cases through the entire tumor process. Using the model utilized by Kostelich et al. [4], I will attempt to redress this deciency. In doing so, I will improve upon a family of models that can be used to approximate the time of development and/or structure evolution in GBMs. The eventual goal is to incorporate Magnetic Resonance Imaging (MRI) data into a parameterized model of GBMs in such a way that it can be used clinically to predict tumor growth and behavior. Furthermore, I hope to come to a denitive conclusion as to the accuracy of the Koteslich et al. model throughout the development of GBMs tumors.
ContributorsManning, Miles (Author) / Kostelich, Eric (Thesis director) / Kuang, Yang (Committee member) / Preul, Mark (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor)
Created2012-12
Description
Microfinance is a term that refers to providing basic financial services to the poor; it has become a powerful tool for poverty alleviation. The idea is a relatively new one - modern microfinance began through experiments in the 1970's - but it has grown quickly and currently serves over 155 million clients worldwide. There are many studies that provide evidence of the positive impact of microfinance and the movement has an array of enthusiastic proponents. It is certainly not the only solution in the battle against poverty, however, and there are also studies that question the true depth of its impact. In looking at microfinance around the globe, one thing becomes clear: although it is an international phenomenon, microfinance has definitely found more success in some regions over others.
ContributorsEdgell, Bailey (Author) / Mendez, Jose (Thesis director) / Schoellman, Todd (Committee member) / Forbes, Stephen (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor)
Created2012-12