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- All Subjects: Biochemistry
- All Subjects: Children's Literature
Description
Identifying disease biomarkers may aid in the early detection of breast cancer and improve patient outcomes. Recent evidence suggests that tumors are immunogenic and therefore patients may launch an autoantibody response to tumor associated antigens. Single-chain variable fragments of autoantibodies derived from regional lymph node B cells of breast cancer patients were used to discover these tumor associated biomarkers on protein microarrays. Six candidate biomarkers were discovered from 22 heavy chain-only variable region antibody fragments screened. Validation tests are necessary to confirm the tumorgenicity of these antigens. However, the use of single-chain variable autoantibody fragments presents a novel platform for diagnostics and cancer therapeutics.
ContributorsSharman, M. Camila (Author) / Magee, Dewey (Mitch) (Thesis director) / Wallstrom, Garrick (Committee member) / Petritis, Brianne (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor) / Virginia G. Piper Center for Personalized Diagnostics (Contributor) / Biodesign Institute (Contributor)
Created2012-12
Description
Children's literature is a comparatively new concept that has changed as the view of children and childhood has changed. The idea that books written for children are more than just amusement and that these books instill values and pride in one's culture has been approached very differently in the United States and Russia. While there are universal morals and common themes in children's literature, there are just as many culturally-dependent ideals that make children's literature and its translation an enlightening way to study the culture of a people or nation and ease the tensions between emerging global and traditional national lessons in children's literature.
ContributorsZubiate, Rachel (Author) / Moldabekova, Saule (Thesis director) / Hoogenboom, Hilde (Committee member) / Hogue, Cynthia (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor)
Created2012-12
Description
Time spent alone is a topic that has been studied in great detail, particularly the manner in which it is spent and the effect it has during the adolescent stage of life. Similarly, stress levels in adolescents have always been a topic of interest because of the effects they could have on the individual later in adulthood. Oddly enough however, the two areas of study have never been looked at in relation to one another. This study will look at different types of alone time as possible stressors in a community sample (N=82) of adolescents transitioning to college. The data on time alone and stress levels was collected through diary reports over a period of 3 days. The analysis only yielded significant effects for females and only for specific categories. It was found that females experience the lowest amount of perceived stress when they are alone and want to be alone, they have more negative affect when their desired environment differs from their current situation, and more positive affect in both the alone incongruence and not alone congruence situations. These results indicate that only women experience stress and affect changes when they encounter different congruent and incongruent environments.
ContributorsVanderwerf, Jennifer (Author) / Doane, Leah (Thesis director) / Knight, George (Committee member) / Arbona, P. Anita (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor)
Created2012-12
Description
The front cover of Uncle What-Is-It is Coming to Visit, a 1993 children’s book by Michael-Willhoite, features two white children frightened by the hairy arm and upturned wrist of an unseen adult. The arm is clad in a frilly pink and orange sleeve, and gaudy bracelets hang from the wrist. The plot hinges on the children’s uncertainty about an uncle they have yet to meet; they know he is gay but are unsure of what it means. Before their mother can explain, she is distracted by a kitchen mishap and the siblings turn to other neighborhood children for answers. They encounter a host of descriptions that terrify them: one neighbor describes gay people as “fags [and] queers [who] really want to be women.” He shows the children a newspaper clipping photo of “a large man dressed in a frilly dress [with] a turban piled high with fruit on his head,” an implicitly racialized caricature reminiscent of Latina style icon Carmen Miranda. Another neighbor describes gay people as “dressed up in black leather. Zippers and chains all over...Dark glasses [and] chaps” (Willhoite, 1993). After having nightmares of men with sinister expressions in tropical-themed drag and leather, the children are overjoyed to discover that their uncle seems “normal.” Relative to depictions of other gay people in the book, Uncle Brett is normal because he is nonthreatening—he is white with short, straight, brown hair; he wears a plain, blue, collared shirt and brown dress pants; he carries a brown briefcase; and he enjoys and excels at activities appropriate for his gender, like catch. Although the book seems to have an affirming message about accepting queer people, it sends a clear message about which queer people are to be feared by children and which are nonthreatening. Nonthreatening queers are those who seem most like people mainstream western society considers normal: people who conform to expected gender roles, who have a vested interest parenting, and who are white and middle-upper class. These nonthreatening queers are by far the most represented in queer-themed literature for children. Based on a survey of 68 children’s books with queer characters, this paper argues that the representation of queer identities in children’s literature upholds more than challenges heteronormativity. I will first address ways many of the books perpetuate gender normativity by problematizing young male characters’ gender-transgressing behavior, portraying queer adults with less threatening gender presentations, and upholding gender binarism; next, I will address how the majority of the books promote repro-narrativity by focusing on monogamous couples’ strong desires and concerted efforts to have/raise children; I will then address race and class and the way white and upper-middle class queer characters are overrepresented while non-white and lower-class queer characters are underrepresented or not represented at all.
ContributorsLester, Jasmine (Author) / Danielson, Marivel (Thesis director) / Blasingame, James (Committee member) / Kuo, Karen (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor)
Created2012-12
Description
The purpose of my honors thesis project was to generate the tools needed for in vivo imaging by determining the optimal plasmid-fluorophore combination. To determine the optimal plasmid and fluorophore, asd plasmids were constructed with various promoters, origins of replications, and red fluorophores. The optimal asd plasmid for fluorescent in vivo imaging was determined by the plasmid stability, growth rate, and growth phase dependence on fluorescent intensity. The end goal is to be able to use the asd plasmid in vaccine strains for the purpose of in vivo imaging of the recombinant attenuated Salmonella vaccine (RASV).
ContributorsEudy, L. Adam (Author) / Curtiss, Roy (Thesis director) / Roland, Kenneth (Committee member) / Forbes, Stephen (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor)
Created2012-12