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Identification of Tumor Associated Antigens using Nucleic Acid Programmable Protein Arrays

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Identifying disease biomarkers may aid in the early detection of breast cancer and improve patient outcomes. Recent evidence suggests that tumors are immunogenic and therefore patients may launch an autoantibody response to tumor associated antigens. Single-chain variable fragments of autoantibodies derived from regional lymph node B cells of breast cancer…

Identifying disease biomarkers may aid in the early detection of breast cancer and improve patient outcomes. Recent evidence suggests that tumors are immunogenic and therefore patients may launch an autoantibody response to tumor associated antigens. Single-chain variable fragments of autoantibodies derived from regional lymph node B cells of breast cancer patients were used to discover these tumor associated biomarkers on protein microarrays. Six candidate biomarkers were discovered from 22 heavy chain-only variable region antibody fragments screened. Validation tests are necessary to confirm the tumorgenicity of these antigens. However, the use of single-chain variable autoantibody fragments presents a novel platform for diagnostics and cancer therapeutics.
ContributorsSharman, M. Camila (Author) / Magee, Dewey (Mitch) (Thesis director) / Wallstrom, Garrick (Committee member) / Petritis, Brianne (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor) / Virginia G. Piper Center for Personalized Diagnostics (Contributor) / Biodesign Institute (Contributor)
Created2012-12
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The Relationship between Stress, Affect and Time Spent Alone in Late Adolescent Males and Females: Congruency vs. Incongruency and Potential Gendered Pathways

Description
Time spent alone is a topic that has been studied in great detail, particularly the manner in which it is spent and the effect it has during the adolescent stage of life. Similarly, stress levels in adolescents have always been a topic of interest because of the effects they could…

Time spent alone is a topic that has been studied in great detail, particularly the manner in which it is spent and the effect it has during the adolescent stage of life. Similarly, stress levels in adolescents have always been a topic of interest because of the effects they could have on the individual later in adulthood. Oddly enough however, the two areas of study have never been looked at in relation to one another. This study will look at different types of alone time as possible stressors in a community sample (N=82) of adolescents transitioning to college. The data on time alone and stress levels was collected through diary reports over a period of 3 days. The analysis only yielded significant effects for females and only for specific categories. It was found that females experience the lowest amount of perceived stress when they are alone and want to be alone, they have more negative affect when their desired environment differs from their current situation, and more positive affect in both the alone incongruence and not alone congruence situations. These results indicate that only women experience stress and affect changes when they encounter different congruent and incongruent environments.
ContributorsVanderwerf, Jennifer (Author) / Doane, Leah (Thesis director) / Knight, George (Committee member) / Arbona, P. Anita (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor)
Created2012-12
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Generation of asd plasmids with a red fluorophore for in vivo imaging

Description
The purpose of my honors thesis project was to generate the tools needed for in vivo imaging by determining the optimal plasmid-fluorophore combination. To determine the optimal plasmid and fluorophore, asd plasmids were constructed with various promoters, origins of replications, and red fluorophores. The optimal asd plasmid for fluorescent in…

The purpose of my honors thesis project was to generate the tools needed for in vivo imaging by determining the optimal plasmid-fluorophore combination. To determine the optimal plasmid and fluorophore, asd plasmids were constructed with various promoters, origins of replications, and red fluorophores. The optimal asd plasmid for fluorescent in vivo imaging was determined by the plasmid stability, growth rate, and growth phase dependence on fluorescent intensity. The end goal is to be able to use the asd plasmid in vaccine strains for the purpose of in vivo imaging of the recombinant attenuated Salmonella vaccine (RASV).
ContributorsEudy, L. Adam (Author) / Curtiss, Roy (Thesis director) / Roland, Kenneth (Committee member) / Forbes, Stephen (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor)
Created2012-12
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The Supermarket Game: An Internet Teaching Tool Designed to Enhance Understanding of Economically Important Food Plants

Description
Plants are essential to human life. They release oxygen into the atmosphere for us to breathe. They also provide shelter, medicine, clothing, tools, and food. For many people, the food that is on their tables and in their supermarkets isn't given much thought. Where did it come from? What part…

Plants are essential to human life. They release oxygen into the atmosphere for us to breathe. They also provide shelter, medicine, clothing, tools, and food. For many people, the food that is on their tables and in their supermarkets isn't given much thought. Where did it come from? What part of the plant is it? How does it relate to others in the plant kingdom? How do other cultures use this plant? The most many of us know about them is that they are at the supermarket when we need them for dinner (Nabhan, 2009) (Vileisis, 2008).
ContributorsBarron, Kara (Author) / Landrum, Leslie (Thesis director) / Swanson, Tod (Committee member) / Pigg, Kathleen (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor)
Created2012-12
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Does Online "Working Out Work" as a Treatment and Prevention for Depression in Older Adults?

Description

RESEARCH QUESTION: Does Online "Working Out Work" as a Treatment and Prevention for Depression in Older Adults? An Analysis of a Prescribed and Monitored Exercise Program Administered via the Internet for Senior Adults with Depression.
OBJECTIVE: The purpose of this study is to investigate and access the effectiveness of an online…

RESEARCH QUESTION: Does Online "Working Out Work" as a Treatment and Prevention for Depression in Older Adults? An Analysis of a Prescribed and Monitored Exercise Program Administered via the Internet for Senior Adults with Depression.
OBJECTIVE: The purpose of this study is to investigate and access the effectiveness of an online prescribed and monitored exercise program for the treatment of depression in Older Adults. The Dependent Variable for the study is Depression. The Independent Variable for the study is the Effects of Exercise administered via the Internet and the population is geriatric adults defined as senior adults aged 50 and older. Depression is defined by Princeton University Scholars (Wordnet, 2006) as a mental state characterized by a pessimistic sense of inadequacy and a despondent lack of activity.
METHODS: The presence and severity of depression will be assessed by using The Merck Manual of Geriatrics (GDS-15) Geriatric Depression Scale. Assessments will be performed at baseline, before and after the treatment is concluded. The subjects will complete the Physical Activity Readiness Questionnaire (PAR-Q) prior to participating in an exercise program three times per week.
LIMITATIONS OF RESEARCH: The limitations of this study are: 1) There is a small sample size limited to Senior Adults aged 50 - 80, and 2) there is no control group with structured activity or placebo, therefore researcher is unable to evaluate if the marked improvement was due to a non-specific therapeutic effect associated with taking part in a social activity (group online exercise program). Further research could compare and analyze the positive effects of a muscular strength training exercise program verses a cardiovascular training exercise program.
ContributorsCaballero-Garcia, Robelyn (Author) / Waldron, Kathleen (Thesis advisor) / College of Liberal Arts and Sciences (Contributor)
Created2011-05-02