Matching Items (38)
Filtering by
- All Subjects: history
- All Subjects: Biochemistry
- All Subjects: Memory
- Creators: School of Historical, Philosophical and Religious Studies
- Creators: Department of Chemistry and Biochemistry
- Member of: Barrett, The Honors College Thesis/Creative Project Collection
Description
This paper aims to investigate how the portrayal of the crusaders in twentieth and twenty-first century film has evolved and how they have become tools in serving contemporary goals, including those of individual filmmakers and broad societal ideologies. Through the analysis of five films, in both narrative and cinematography, spanning from the 1950s until 2011, themes of redemption, maturity, and the dichotomy of "good" and "bad" are discussed, as well as their chronological evolution in regards to the crusading hero. These films, widely ranging in historical subject matter and country of origin, show a greater range of evolution for the holy war hero and the important themes widely associated with them.
ContributorsBowman, Taylor Nicole (Author) / Benkert, Volker (Thesis director) / Bruhn, Karen (Committee member) / Miller, April (Committee member) / Barrett, The Honors College (Contributor) / School of Historical, Philosophical and Religious Studies (Contributor)
Created2015-05
Description
Using DNA nanotechnology a library of structures of various geometries have been built; these structures are modified chemically and/or enzymatically at nanometer precisions. With DNA being chemically very stable, these structures can be functionalized through an abundance of well-established protocols. Additionally, they can be used for various biological and medicinal purposes, such as drug delivery. For in vivo applications, the DNA nanostructures must have a long circulation life in the bloodstream; otherwise, they could be easily excreted shortly after entry. One way of making these nanostructures long lasting in the blood is to cover them with the biocompatible polymer, polyethylene glycol (PEG). Adding DNA to PEG before forming structures has been found to interfere in the hybridization of the DNA in the structure, resulting in formation of deformed structures. In this study we have developed a new methodology based on "click chemistry" (CC) to modify the surface of DNA nanostructures with PEG after they are formed. These structures can then be used for in vivo studies and potential applications in the future.
ContributorsSmith, Eric Lynn (Author) / Yan, Hao (Thesis director) / Liu, Yan (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor)
Created2015-05
Description
The aim of this thesis is to explore the relationship between architecture and history in Virginia from 1607 to the eve of the American Revolution to create a complete historical narrative. The interdependency of history and architecture creates culturally important pieces and projects the colonist's need to connect to the past as well as their innovations in their own cultural exploration. The thesis examines the living conditions of the colonists that formed Jamestown, and describes the architectural achievements and the historical events that were taking place at the time. After Jamestown, the paper moves on to the innovations of early Virginian architecture from Colonial architecture to Georgian architecture found in Williamsburg. Conclusively, the thesis presents a historical narrative on how architecture displays a collection of ideals from the Virginian colonists at the time. The external display of architecture parallels the events as well as the economic conditions of Virginia, creating a social dialogue between the gentry and the common class in the colony of Virginia.
ContributorsChang, Hosu (Author) / Gray, Susan (Thesis director) / O'Donnell, Catherine (Committee member) / Barrett, The Honors College (Contributor) / School of Historical, Philosophical and Religious Studies (Contributor) / School of Social Transformation (Contributor)
Created2015-05
Description
Three populations of experimentally evolved Drosophila melanogaster populations made up of high temperature (H, constant 25 ᵒC), low temperature (C, constant 16 ᵒC) and temporal homogeneity (T, environment changes between 16 ᵒC and 25 ᵒC) were prepared and assayed to determine difference in citrate synthase activity. Between the three groups, the results were inconclusive: the resulting reaction rates in units of nmol min-1mgfly-1 were 81.8 + 20.6, 101 + 15.6, and 96.9 + 25.2 for the hot (H), cold (C), and temporally homogeneous (T) groups, respectively. We conclude that the high associated variability was due to a lack of control regarding the collection time of the experimentally evolved Drosophila.
ContributorsBelohlavek, David (Author) / Angilletta, Michael (Thesis director) / Francisco, Wilson (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor)
Created2015-05
Description
Guided by the Obama administration, NASA has begun developing commercial launch capabilities. For both cargo and crew delivery to the International Space Station, NASA has selected companies to build and operate the vehicles at a fixed price. Alexander McDonald suggests that this continues a trend in space exploration established by large observatory projects, and that the Apollo-era style of funding and operation was a historical anomaly. This paper attempts to discover if historical analog can support or weaken this thesis. The analogs chosen are two episodes in the history of terrestrial exploration: the experience of the Spanish and British empires in North America. These are compared to the history of space exploration up until today, focusing on how the role of private enterprise has changed in each instance. While the analogies between historical episodes are weak in a few areas, they do possess a common narrative concerning the shifting balance between private and government interests. This narrative supports McDonald's thesis, and shows that NASA's current policy anticipates an expected transition towards a private-public hybrid model of exploration and expansion.
ContributorsRobb, Daniel Robert (Author) / Pyne, Stephen (Thesis director) / Bell, Jim (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor) / School of Historical, Philosophical and Religious Studies (Contributor)
Created2015-05
Exploring Structure and Function of Human Cold Sensing Protein TRPM8 with ROSETTA Comparative Models
Description
Transient Receptor Potential (TRP) channels are a diverse class of ion channels notable as polymodal sensors. TRPM8 is a TRP channel implicated in cold sensation, nociception, and a variety of human diseases, including obesity and cancer. Despite sustained interest in TRPM8 since its discovery in 2001, many of the molecular mechanisms that underlie function are not yet clear. Knowledge of these properties could have implications for medicine and physiological understanding of sensation and signaling. Structures of TRP channels have proven challenging to solve, but recent Cryoelectron microscopy (Cryo-EM) structures of TRPV1 provide a basis for homology-based modeling of TRP channel structures and interactions. I present an ensemble of 11,000 Rosetta computational homology models of TRPM8 based on the recent Cryo-EM apo structure of TRPV1 (PDB code:3J5P). Site-directed mutagenesis has provided clues about which residues are most essential for modulatory ligands to bind, so the models presented provide a platform to investigate the structural basis of TRPM8 ligand modulation complementary to existing functional and structural information. Menthol and icilin appear to interact with interfacial residues in the sensor domain (S1-S4). One consensus feature of these sites is the presence of local contacts to the S4 helix, suggesting this helix may be mechanistically involved with the opening of the pore. Phosphatidylinositol 4,5-bisphosphate (PIP2)has long been known to interact with the C-terminus of TRPM8, and some of the homology models contain plausible binding pockets where PIP2 can come into contact with charged residues known to be essential for PIP2 modulation. Future in silico binding experiments could provide testable hypothesis for in vitro structural studies, and experimental data (e.g. distance constraints from electron paramagnetic resonance spectroscopy [EPR]) could further refine the models.
ContributorsHelsell, Cole Vincent Maher (Author) / Van Horn, Wade (Thesis director) / Wang, Xu (Committee member) / Barrett, The Honors College (Contributor) / School of Mathematical and Statistical Sciences (Contributor) / Department of Chemistry and Biochemistry (Contributor)
Created2015-05
Description
Memory Wipe is a 22 minute, video art piece that utilizes home movie footage filmed on VHS and 8mm, as well as television and cartoon ephemera, to explore the way in which personal memory is constructed and altered through the process of recording and viewing. Three recent events in my life inspired work: the discovery of a box containing my favorite childhood media, the revelation that I am the last male of my family, and the impending sale of my family's farmland. My mother never used a video camera, insisting that her childhood was lost in footage filmed but never watched. It should also be noted that not once do I appear in this piece; therefore, I decided to extract myself from the narrative. Rather than simply guide the audience along with anecdotes from my life, I instead invite viewers to draw their own meanings and create their own nostalgias from the piece. Originally, Memory Wipe was to be accompanied by live narration, but all things considered, I thought I would let it speak for itself. Video link: https://www.youtube.com/watch?v=-E42a6Koma4
ContributorsMcDougall, Clayton Ross (Author) / Magenta, Muriel (Thesis director) / Brye, Anne (Committee member) / Barrett, The Honors College (Contributor) / School of Film, Dance and Theatre (Contributor) / School of Historical, Philosophical and Religious Studies (Contributor)
Created2015-05
Description
Since Dylan Roof, a white supremacist, shot and killed nine members of a black church in Charleston on June 17, 2015, Confederate symbols have stood at the center of much controversy across the United States. Although the Confederate battle flag remains the most obvious example, the debate took a particular form in Tennessee, centering on the image of General Nathan Bedford Forrest. Born in 1822 to a poor family, he left school early to work. Although his work in the slave trade made him a millionaire, his later participation in the massacre of over 300 black soldiers at Fort Pillow in 1864 during the Civil War and association with the Ku Klux Klan cemented his reputation as a violent racist. Yet, many white Tennesseans praised him as a hero and memorialized him. This thesis examines Nathan Bedford Forrest State Park in Benton County and Forrest Park, now Health Sciences Park, in Memphis to examine what characteristics denote a controversial memorial. Specifically, I focus on the physical form, the location, and the demographics of the area, investigating how these components work together to give rise to controversy or acceptance of the memorial's image. Physical representations greatly impact the ideas associated with the memorial while racial demographics affect whether or not Forrest's representation as a hero speaks true to modern interpretations and opinions.
ContributorsFish, Caroline Rose (Author) / Longley, Rodney (Thesis director) / Suk, Mina (Committee member) / School of Historical, Philosophical and Religious Studies (Contributor) / School of Politics and Global Studies (Contributor) / School of International Letters and Cultures (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
This research looks at the state of Anglo-American political relations since 1980. By examining the political partnerships between Ronald Reagan and Margaret Thatcher, Margaret Thatcher and George H.W. Bush, George W. Bush and Tony Blair, Barack Obama and Gordon Brown, and Barack Obama and David Cameron, it explores if the so called ‘special relationship’ remains so special today in a world of growing political animosity and challenges. The thesis argues that the success of the ‘special relationship’ between the United States and United Kingdom has not been just due to similar political ideologies or goals, but also personal friendships which often overcame national interests or immediate personal political gain. Furthermore, it is often the periods of disagreement between these sets of leaders that helped strengthen the relationship between America and Britain, evidenced by episodes like the Falklands War, policy towards the Soviet Union, the invasion of Grenada, and the wars in Iraq and Afghanistan. Ultimately, the thesis explores how current relations have deteriorated due to problems on both sides of the Atlantic under the Obama, Brown, and Cameron administrations, but the research concludes that the special relationship is, while damaged, alive and fixable.
ContributorsGavin, Stephen Christopher (Author) / Critchlow, Donald (Thesis director) / Stermetz, Catherine (Committee member) / School of Politics and Global Studies (Contributor) / School of Historical, Philosophical and Religious Studies (Contributor) / Barrett, The Honors College (Contributor)
Created2015-12
Description
Quercetin 2,3-dioxygenase from Bacillus subtilis has been identified and characterized as the first known prokaryotic quercetinase. This enzyme catalyzes the cleavage of the O-heteroaromatic ring of the flavonol quercetin to the corresponding depside and carbon monoxide. The first quercetinase was characterized from a species of Aspergillus genus, and was found to contain one Cu2+ per subunit. For many years, it was thought that the B. subtilis quercetinase contained two Fe2+ ions per subunit; however, it has since been discovered that Mn2+ is a much more likely cofactor. Studies of overexpressed bacterial enzyme in E. coli indicated that this enzyme may be active with other metal ions (e.g. Co2+); however, the production of enzyme with full metal incorporation has only been possible with Mn2+. This study explores the notion that metal manipulation after translation, by partially unfolding the enzyme, chelating the metal ions, and then refolding the protein in the presence of an excess of divalent metal ions, could generate enzyme with full metal occupancy. The protocols presented here included testing for activity after incubating purified quercetinase with EDTA, DDTC, imidazole and GndHCl. It was found that the metal chelators had little to no effect on quercetinase activity. Imidazole did appear to inhibit the enzyme at concentrations in the millimolar range. In addition, the quercetinase was denatured in GndHCl at concentrations above 1 M. Recovering an active enzyme after partial or complete unfolding proved difficult, if not impossible.
ContributorsKrojanker, Elan Daniel (Author) / Francisco, Wilson (Thesis director) / Allen, James P. (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor)
Created2014-05