Filtering by
- All Subjects: Stress
- All Subjects: Anxiety
- Creators: Department of Psychology
![148007-Thumbnail Image.png](https://d1rbsgppyrdqq4.cloudfront.net/s3fs-public/styles/width_400/public/2021-07/148007-Thumbnail%20Image.png?versionId=OB1VNNoGFdBIySha_4cKM3eVEwkpuXR9&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIASBVQ3ZQ42ZLA5CUJ/20240608/us-west-2/s3/aws4_request&X-Amz-Date=20240608T064517Z&X-Amz-SignedHeaders=host&X-Amz-Expires=120&X-Amz-Signature=1deb5e6e05a58c0245072c1822a967b4dac324656e07a056d502b459a22b858b&itok=TQ3R93r4)
Exploration of a mouse model (C57BL/6J) capable of demonstrating behavioral changes after adolescent social isolation that are consistent with prior findings may prove beneficial in later research. This study examined 2 proposed long-term effects of isolated housing (one mouse/cage), when compared to group housing (two mice/cage) during adolescence. Mice were placed in their respective housing conditions after weaning (PND 21) and remained in those conditions until PND 60. The same cohorts were used in both phases of the experiment. Phase 1 sought to confirm previous findings that showed increases in ethanol intake after adolescent social isolation using a 2-bottle preference Drinking-in-the-Dark (DID) design over a 4-day period (PND 64-PND 67.). Phase 2 sought to elucidate the effects present after adolescent social isolation, as measured using response inhibition capabilities demonstrated during fixed-minimum interval (FMI) trials (PND 81-PND 111). Findings in phase 1 of the experiment were non-significant, save a strong tendency for female mice in both housing conditions to drink more as a proportion of their bodyweight (g/kg). However, a trend of lower bodyweight in single housed mice did exist, which does suggest that detrimental stress was applied via the used of adolescent isolation in that housing condition. Findings in phase 2 showed little effect of adolescent social isolation on mean inter-response time (IRT) at any criterion used (FMI-0, FMI-4, FMI-6). Evaluation of mean interquartile range (IQR) of IRTs showed a significantly greater amount of variation in IRT responses within single housed mice at the highest criterion (FMI-6), and a trend in the same direction when FMI-4 and FMI-6 were tested concurrently. Taken as a whole, the findings of this experiment suggest that the effect of adolescent social isolation on ethanol intake is far less robust than the effect of sex and may be difficult to replicate in a low-power study. Additionally, adolescent social isolation may interfere with the ability of mice to show consistent accuracy during FMI tasks or a delay in recognition of FMI criterion change.
![136651-Thumbnail Image.png](https://d1rbsgppyrdqq4.cloudfront.net/s3fs-public/styles/width_400/public/2021-05/136651-Thumbnail%20Image.png?versionId=GTa4YfKKlIUSgcp5AlWtP56se3iCtf5w&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIASBVQ3ZQ42ZLA5CUJ/20240614/us-west-2/s3/aws4_request&X-Amz-Date=20240614T235657Z&X-Amz-SignedHeaders=host&X-Amz-Expires=120&X-Amz-Signature=7811f177bf866873bc87e66669fe3561b72080dacbf58f68094c55c5fd17971d&itok=LiaWhetX)
![137784-Thumbnail Image.png](https://d1rbsgppyrdqq4.cloudfront.net/s3fs-public/styles/width_400/public/2021-05/137784-Thumbnail%20Image.png?versionId=B4CUfdDX5dgky583b7vO1V_8Hw.9ekeH&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIASBVQ3ZQ42ZLA5CUJ/20240614/us-west-2/s3/aws4_request&X-Amz-Date=20240614T235657Z&X-Amz-SignedHeaders=host&X-Amz-Expires=120&X-Amz-Signature=10edfef564b9db8ddc255c3c74d7bafcbb595bc356f006d10803199565dca58e&itok=hbGTQ011)
![137245-Thumbnail Image.png](https://d1rbsgppyrdqq4.cloudfront.net/s3fs-public/styles/width_400/public/2021-05/137245-Thumbnail%20Image.png?versionId=_8ZRk69AX24lku3vID3NrxsaX.1_MqCR&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIASBVQ3ZQ42ZLA5CUJ/20240615/us-west-2/s3/aws4_request&X-Amz-Date=20240615T010329Z&X-Amz-SignedHeaders=host&X-Amz-Expires=120&X-Amz-Signature=9ba21c1e7ad14586a1f55ff15e1cec7c0acbec647977a0fd9df21c7d8165ec89&itok=Fva5KACu)
![137533-Thumbnail Image.png](https://d1rbsgppyrdqq4.cloudfront.net/s3fs-public/styles/width_400/public/2021-05/137533-Thumbnail%20Image.png?versionId=.VwBvYkn_XX0NRIuCvsw3N.3bJBNlWaE&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIASBVQ3ZQ42ZLA5CUJ/20240614/us-west-2/s3/aws4_request&X-Amz-Date=20240614T115944Z&X-Amz-SignedHeaders=host&X-Amz-Expires=120&X-Amz-Signature=ef134961f299c6ac422afeffb991455d18a834af1b60cd10e6c9aaa7e911d4eb&itok=iDbyFgNE)
![135851-Thumbnail Image.png](https://d1rbsgppyrdqq4.cloudfront.net/s3fs-public/styles/width_400/public/2021-05/135851-Thumbnail%20Image.png?versionId=3I5fGo_0lllFj0Upyf95jSozZL32iZlv&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIASBVQ3ZQ42ZLA5CUJ/20240614/us-west-2/s3/aws4_request&X-Amz-Date=20240614T161401Z&X-Amz-SignedHeaders=host&X-Amz-Expires=120&X-Amz-Signature=badd25b27a9195b5ebcd92415ce42a5c6b40e765b469d916add30c910d970471&itok=WHBp248a)
![130879-Thumbnail Image.png](https://d1rbsgppyrdqq4.cloudfront.net/s3fs-public/styles/width_400/public/2021-07/130879-Thumbnail%20Image.png?versionId=cEpl5dV9yWTWLxPGUhAfG2MpwPauG_vS&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIASBVQ3ZQ42ZLA5CUJ/20240610/us-west-2/s3/aws4_request&X-Amz-Date=20240610T011023Z&X-Amz-SignedHeaders=host&X-Amz-Expires=120&X-Amz-Signature=48331c7bc88e9aecf3a1f6fc9690738256e8d6b30113529c23bd30877e0b0a56&itok=rTrYR2_G)
![132004-Thumbnail Image.png](https://d1rbsgppyrdqq4.cloudfront.net/s3fs-public/styles/width_400/public/2021-07/132004-Thumbnail%20Image.png?versionId=4GKSZDYI3v95_3pkJXhoGkpukB5igyY4&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIASBVQ3ZQ42ZLA5CUJ/20240615/us-west-2/s3/aws4_request&X-Amz-Date=20240615T000933Z&X-Amz-SignedHeaders=host&X-Amz-Expires=120&X-Amz-Signature=1d7d915493e0770397df44397c2c616855c76e8da4d056fb13c5e7bb9b5ca519&itok=aPnwXcps)
![132097-Thumbnail Image.png](https://d1rbsgppyrdqq4.cloudfront.net/s3fs-public/styles/width_400/public/2021-08/132097-Thumbnail%20Image.png?versionId=lLytQcdP0idTQoH32Y7G3YLLrS1.mUWH&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIASBVQ3ZQ42ZLA5CUJ/20240614/us-west-2/s3/aws4_request&X-Amz-Date=20240614T090027Z&X-Amz-SignedHeaders=host&X-Amz-Expires=120&X-Amz-Signature=e1d42785cc434633694be4b00911dcba650e2027f923436b3cebbf9a563fa56d&itok=Usgp0tR3)
Squeakie is a positive influence on the way the other characters perceive themselves. The shadow turns each character’s self-doubt and negative feelings into fuel, which he stores in a paintbrush. When he takes the fuel from the character, it fades their body color. Phan has anxiety and uses the 4-7-8 breathing technique to overcome her panic attacks. Her range of color is blue to light blue. Ovid feels guilty and exercises to take his mind off his guilty thoughts. Ovid is either red or light pink. Amelia is depressed and reframes her way of thinking to overcome her inability to fly. Visually she is green or light green. The shadow is later revealed as a misguided character who was just looking to escape the dream world and find friends.
The story is resolved by the stuffed animals joining forces with the perceived antagonist, the shadow, to operate a plane. They each use their strength of color to fuel the plane, which takes them back to the real world. When Sophia’s mom pulls the stuffed animals out of the washer, the shadow comes with them. The shadow, now a cat with rainbow patches, is instantly loved by Sophia. The story ends with the stuffed animals drying on the porch bench while Sophia plays with the shadow in the new backyard.