This thesis discusses the importance and impact of preserving Italian culture, food traditions, and local identity. Reflecting on a year spent in Italy during the 2021-2022 academic school year, the author explores the significance and preservation of Italian culture, food traditions, and local identities. The thesis identifies three compelling rationales for their preservation: the health benefits derived from traditional practices, the positive environmental implications of maintaining biodiversity and sustainable methods, and the societal advantages of connected communities fostering a sense of belonging and purpose. Then, this thesis will examine how the nonprofit organization Slow Food, renowned for its commitment to preserving culinary heritage, raises awareness about the imperative need for preservation and education. The organization's core principles and ongoing initiatives serve as a model for championing these essential causes. Using academic analyses of the intersection between food and culture, this thesis establishes a comprehensive analysis of their connection and the significance of their preservation. This thesis draws on diverse sources, including the author's personal experiences during their study-abroad program in Italy. Through these lenses, the paper underscores the critical importance of upholding Italian culture and food traditions amidst evolving global food systems.

Agassiz’s desert tortoise (Gopherus agassizii) is a long-lived species native to the Mojave Desert and is listed as threatened under the US Endangered Species Act. To aid conservation efforts for preserving the genetic diversity of this species, we generated a whole genome reference sequence with an annotation based on deep transcriptome sequences of adult skeletal muscle, lung, brain, and blood. The draft genome assembly for G. agassizii has a scaffold N50 length of 252 kbp and a total length of 2.4 Gbp. Genome annotation reveals 20,172 protein-coding genes in the G. agassizii assembly, and that gene structure is more similar to chicken than other turtles. We provide a series of comparative analyses demonstrating (1) that turtles are among the slowest-evolving genome-enabled reptiles, (2) amino acid changes in genes controlling desert tortoise traits such as shell development, longevity and osmoregulation, and (3) fixed variants across the Gopherus species complex in genes related to desert adaptations, including circadian rhythm and innate immune response. This G. agassizii genome reference and annotation is the first such resource for any tortoise, and will serve as a foundation for future analysis of the genetic basis of adaptations to the desert environment, allow for investigation into genomic factors affecting tortoise health, disease and longevity, and serve as a valuable resource for additional studies in this species complex.

Data Availability: All genomic and transcriptomic sequence files are available from the NIH-NCBI BioProject database (accession numbers PRJNA352725, PRJNA352726, and PRJNA281763). All genome assembly, transcriptome assembly, predicted protein, transcript, genome annotation, repeatmasker, phylogenetic trees, .vcf and GO enrichment files are available on Harvard Dataverse (doi:10.7910/DVN/EH2S9K).