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- Creators: Barrett, The Honors College
- Member of: Barrett, The Honors College Thesis/Creative Project Collection
- Member of: Theses and Dissertations
The aim of this creative project was to explore the ideas of impermanence and transience through the lens of different, largely non-western cultural backgrounds, and to incorporate what I learned into my own work as a painter. As part of this, I focused on the materials, techniques, visual strategies, and philosophies that guided the creation of these works. The project consisted of a discrete research phase, during which time I gathered information and materials related to my topic, and a creation phase, when I focused largely on the production of oil paintings and ink paintings whose technique and/or subject matter pertained to impermanence. Research for the most part was conducted by utilizing online and physical collections of work to analyze the formal elements of the work along with the cultural context in which it was created. Ultimately the creative project resulted in a product of three oil paintings and five ink paintings.
Glioblastoma (GB) is one of the deadliest cancers and the most common form of adult primary brain tumors. SGEF (ARHGEF26) has been previously shown to be overexpressed in GB tumors, play a role in cell invasion/migration, and increase temozolomide (TMZ) resistance.[3] It was hypothesized parental LN229 cell lines with SGEF knockdown (LN229-SGEFi) will show decreased metabolism in the MTS assay and decreased colony formation in a colony formation assay compared to parental LN229 cells after challenging the two cell lines with TMZ. For WB and co-immunoprecipitation (co-IP), parental LN229 cells with endogenous SGEF and BRCA were expected to interact and stain in the BRCA1:IP WB. LN229-SGEFi cells were expected to show very little SGEF precipitated due to shRNA targeted knockdown of SGEF. In conditions with mutations in the BRCA1 binding site (LN229-SGEFi + AdBRCAm/AdDM), SGEF expression was expected to decrease compared to parental LN229 or LN229-SGEFi cells reconstituted with WT SGEF (LN229-SGEFi + AdWT). LN229 infected with AdSGEF with a mutated nuclear localization signal (LN229-SGEFi + AdNLS12m) were expected to show BRCA and SGEF interaction since whole cell lysates were used for the co-IP. MTS data showed no significant differences in metabolism between the two cell lines at all three time points (3, 5, and 7 days). Western blot analysis was successful at imaging both SGEF and BRCA1 protein bands from whole cell lysate. The CFA showed no significant difference between cell lines after being challenged with 500uM TMZ. The co-IP immunoblot showed staining for BRCA1 and SGEF for all lysate samples, including unexpected lysates such as LN229-SGEFi, LN229-SGEFi + AdBRCAm, and LN229-SGEFi + AdDM. These results suggested either an indirect protein interaction between BRCA1 and SGEF, an additional BRCA binding site not included in the consensus, or possible detection of the translocated SGEF in knockdown cells lines since shRNA cannot enter the nucleus. Further optimization of CO-IP protocol, MTS assay, and CFA will be needed to characterize the SGEF/BRCA1 interaction and its role in cell survival.
Cancer rates vary between people, between cultures, and between tissue types, driven by clinically relevant distinctions in the risk factors that lead to different cancer types. Despite the importance of cancer location in human health, little is known about tissue-specific cancers in non-human animals. We can gain significant insight into how evolutionary history has shaped mechanisms of cancer suppression by examining how life history traits impact cancer susceptibility across species. Here, we perform multi-level analysis to test how species-level life history strategies are associated with differences in neoplasia prevalence, and apply this to mammary neoplasia within mammals. We propose that the same patterns of cancer prevalence that have been reported across species will be maintained at the tissue-specific level. We used a combination of factor analysis and phylogenetic regression on 13 life history traits across 90 mammalian species to determine the correlation between a life history trait and how it relates to mammary neoplasia prevalence. The factor analysis presented ways to calculate quantifiable underlying factors that contribute to covariance of entangled life history variables. A greater risk of mammary neoplasia was found to be correlated most significantly with shorter gestation length. With this analysis, a framework is provided for how different life history modalities can influence cancer vulnerability. Additionally, statistical methods developed for this project present a framework for future comparative oncology studies and have the potential for many diverse applications.
The goal of this project was to design and create a genetic construct that would allow for <br/>tumor growth to be induced in the center of the wing imaginal disc of Drosophila larvae, the <br/>R85E08 domain, using a heat shock. The resulting transgene would be combined with other <br/>transgenes in a single fly that would allow for simultaneous expression of the oncogene and, in <br/>the surrounding cells, other genes of interest. This system would help establish Drosophila as a <br/>more versatile and reliable model organism for cancer research. Furthermore, pilot studies were <br/>performed, using elements of the final proposed system, to determine if tumor growth is possible <br/>in the center of the disc, which oncogene produces the best results, and if oncogene expression <br/>induced later in development causes tumor growth. Three different candidate genes were <br/>investigated: RasV12, PvrACT, and Avli.
The Student Art Project is art patronage for the 21st century—a curated online gallery featuring exceptional student artists. The Student Art Project is a highly curated experience for buyers. Only five artists are featured each month. Buyers are not bombarded with thousands of different products and separate artists “shops”. They can read artists bios and find art they connect with.
Student artists apply through an online form. Once accepted to the program, artists receive a $200 materials stipend to create an exclusive collection of 5-10 pieces. Original artwork and limited edition prints are sold through our website. These collections can potentially fund an entire year of college tuition, a life-changing amount for many students.
Brick-and-mortar galleries typically take 40-60% of the retail price of artwork. The Student Art Project will only take 30%, which we will use to reinvest in future artists. Other art websites, like Etsy, require the artists to ship, invoice, and communicate with customers. For students, this means less time spent in the classroom and less time developing their craft. The Student Art Project handles all business functions for our artists, allowing them to concentrate on what really matters, their education.
Anthropocentric society faces a multiplicity of environmental challenges, catalyzed and perpetuated by urban-industrial culture. Many of today’s perspectives and sustainable strategies cannot accommodate the challenges’ inherent complexity. Because urban-industrial society is only projected to grow, both in enormity and influence, the only viable option is to elucidate the complexity and employ it.
A potential setting in which to frame this exploration is the intersection of urbanism, landscape, and ecology –an overlap first introduced by the theories of Landscape Urbanism and Ecological Urbanism. Here, urbanization is not just discussed as an isolated phenomenon but one that is embedded within and responding to a variety of systems and scales. The methodologies of Landscape Urbanism and Ecological Urbanism also acknowledge artists and the visual arts as invaluable tools for realizing, communicating, and inspiring the new perspectives and modes of intervention needed to address the aforementioned urban complexity. Such artists who operate within this realm include Sissel Tolaas, Maya Lin, Katrin Sigurdardottir, David Maisel, Olafur Eliason, Mierle Ukeles, Suzanne Lacy, Steve Rowell, Mel Chin, and the Center for Land Use Interpretation. Case study analyses reveal many of these artists begin their investigations with provocative, searching questions situated within the realms of urbanism, landscape, and ecology. This is proceeded by relative scientific research and/or community involvement or outreach. Furthermore, the artists work within and extrapolate from a variety of other disciplines —increasing the scope and applicability of their work. The information they collect via this multidisciplinary approach is then metaphorically translated to the visual arts, where the public can not only physically or sensorially experience it, but understand and deduce its meaning and significance: public awareness being one of the more essential aspects of a sustainable society and at the root of our current struggle.
As a designer and architect, I will engage the artist’s mindset to explore the current and complex issue of resource extraction within Superior, Arizona: a topic at the core of urbanism, landscape, and ecology. While the town is not considered "urban" by standard definition, it and its surrounding landscapes are indirectly sculpted by the needs of urban society —rendering it the setting for this application. Within a group, we will begin with a searching question. We will conduct relative scientific research, engage the community of Superior, and call upon a variety of other disciplines to aid and inform our work. Through metaphor, the research and resulting discoveries will be artistically represented and composed within a designed exhibition of hopeful “things” (See Bruno Latour, “From Realpolitik to Dingpolitik”). This exhibition will theoretically take place on Superior’s currently dilapidated Main Street, amid a more accessible sphere. The eventual goal of the project is to illuminate and understand the complexities of resource extraction, specifically within Superior, while also enabling public awareness and empowerment through lucidity and comprehension.
