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- All Subjects: Social Media
- Creators: Barrett, The Honors College
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Description
Introduction: Human papillomavirus (HPV) infection is seen in up to 90% of cases of cervical cancer, the third leading cancer cause of death in women. Current HPV screening focuses on only two HPV types and covers roughly 75% of HPV-associated cervical cancers. A protein based assay to test for antibody biomarkers against 98 HPV antigens from both high and low risk types could provide an inexpensive and reliable method to screen for patients at risk of developing invasive cervical cancer. Methods: 98 codon optimized, commercially produced HPV genes were cloned into the pANT7_cGST vector, amplified in a bacterial host, and purified for mammalian expression using in vitro transcription/translation (IVTT) in a luminescence-based RAPID ELISA (RELISA) assay. Monoclonal antibodies were used to determine immune cross-reactivity between phylogenetically similar antigens. Lastly, several protein characteristics were examined to determine if they correlated with protein expression. Results: All genes were successfully moved into the destination vector and 86 of the 98 genes (88%) expressed protein at an adequate level. A difference was noted in expression by gene across HPV types but no correlation was found between protein size, pI, or aliphatic index and expression. Discussion: Further testing is needed to express the remaining 12 HPV genes. Once all genes have been successfully expressed and purified at high concentrations, DNA will be printed on microscope slides to create a protein microarray. This microarray will be used to screen HPV-positive patient sera for antibody biomarkers that may be indicative of cervical cancer and precancerous cervical neoplasias.
ContributorsMeshay, Ian Matthew (Author) / Anderson, Karen (Thesis director) / Magee, Mitch (Committee member) / Katchman, Benjamin (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor)
Created2015-05
Description
Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related mortality in the USA and throughout the world. Two phenotypes that promote this deadly outcome are the invasive potential of NSCLC and the emergence of therapeutic resistance in this disease. There is an unmet clinical need to understand the mechanisms that govern NSCLC cell invasion and therapeutic resistance, and to target these phenotypes towards abating the dismal five-year survival of NSCLC. The expression of the tumor necrosis factor receptor superfamily, member 12A (TNFRSF12A; Fn14) correlates with poor patient survival and invasiveness in many tumor types including NSCLC. We hypothesize that suppression of Fn14 will inhibit NSCLC cell motility and reduce cell viability. Here we demonstrate that atorvastatin calcium treatment reduces Fn14 expression in NSCLC cell lines. Prior to Fn14 protein suppression, atorvastatin calcium modulated the expression of the Fn14 modulators P-ERK1/2 and P-NF-κβ. Atorvastatin calcium treatment inhibited the migratory capacity in H1975, H2030 and H1993 cells by at least 55%. When chemotactic migration in H2030 cells was induced by the Fn14 ligand TNF-like weak inducer of apoptosis (TWEAK) treatment, atorvastatin calcium successfully negated any stimulatory effects. Inversely, treatment of NSCLC cells with cholesterol resulted in a statistically significant increase in migration. Depletion of Fn14 expression via siRNA suppressed the migratory effect of cholesterol. Finally, atorvastatin calcium treatment sensitized cells to radiation treatment, reducing cell survival. These data suggest that atorvastatin calcium may inhibit NSCLC invasiveness through a mechanism involving Fn14, and may be a novel therapeutic target in NSCLC tumors expressing Fn14.
ContributorsCornes, Victoria Elisabeth (Author) / Stout, Valerie (Thesis director) / Whitsett, Timothy (Committee member) / Carson, Vashti (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor)
Created2015-05
Description
Abstract Molecular Engineering of Novel Polymeric Agents for Targeted Cancer Gene Therapy Dana Matthews Cancer gene cell therapy is a strategy that involves the administration of genes for correcting the effect of mutated cancer cells in order to induce tumor cell death. In particular, genes that encode for pro-apoptotic proteins can result in death of tumor cells. Prostate cancer is a very common cancer among males in America, and as highly destructive chemotherapy and radiation are generally the only treatments available once the cancer has metastasized, there is a need for the development of treatments that can specifically target and kill prostate cancer cells, while demonstrating low toxicity to other tissue. This experiment will attempt to create such a treatment through gene therapy techniques. The parallel synthesis and DNA binding affinity assay utilized in these experiments have produced a polymer that surpasses pEI-25, a gene delivery polymer standard, in both transfection efficacy and low cytotoxicity and trafficking of polyplexes in the cell, and finding methods to increase the transfection efficacy and specificity of polyplexes for PC3-PSMA cells.
ContributorsMatthews, Dana (Author) / Rege, Kaushal (Thesis director) / Linton, Rebecca (Committee member) / Huang, Huang-Chial (Committee member) / Barrett, The Honors College (Contributor)
Created2008-12
Description
Despite the 40-year war on cancer, very limited progress has been made in developing a cure for the disease. This failure has prompted the reevaluation of the causes and development of cancer. One resulting model, coined the atavistic model of cancer, posits that cancer is a default phenotype of the cells of multicellular organisms which arises when the cell is subjected to an unusual amount of stress. Since this default phenotype is similar across cell types and even organisms, it seems it must be an evolutionarily ancestral phenotype. We take a phylostratigraphical approach, but systematically add species divergence time data to estimate gene ages numerically and use these ages to investigate the ages of genes involved in cancer. We find that ancient disease-recessive cancer genes are significantly enriched for DNA repair and SOS activity, which seems to imply that a core component of cancer development is not the regulation of growth, but the regulation of mutation. Verification of this finding could drastically improve cancer treatment and prevention.
ContributorsOrr, Adam James (Author) / Davies, Paul (Thesis director) / Bussey, Kimberly (Committee member) / Barrett, The Honors College (Contributor) / School of Mathematical and Statistical Sciences (Contributor) / Department of Chemistry and Biochemistry (Contributor) / School of Life Sciences (Contributor)
Created2015-05
Description
The selfie craze has taken over smartphone users. Despite a wealth of published opinion pieces on the matter, there lacks a constructive and academically-based dialogue about selfies. "Presentation of Selfie" is a creative, interactive space that analyzes a sample of collected selfies. The project takes a look at how age, relationships and identities play a role in the social significance of selfie culture.
ContributorsJeffords, Alison Rae (Author) / McGibbney-Vlahoulis, Michelle (Thesis director) / Leong, Karen (Committee member) / Barrett, The Honors College (Contributor) / School of Social Transformation (Contributor) / Department of Marketing (Contributor)
Created2015-05
Description
Cancer remains one of the leading killers throughout the world. Death and disability due to lung cancer in particular accounts for one of the largest global economic burdens a disease presents. The burden on third-world countries is especially large due to the unusually large financial stress that comes from late tumor detection and expensive treatment options. Early detection using inexpensive techniques may relieve much of the burden throughout the world, not just in more developed countries. I examined the immune responses of lung cancer patients using immunosignatures – patterns of reactivity between host serum antibodies and random peptides. Immunosignatures reveal disease-specific patterns that are very reproducible. Immunosignaturing is a chip-based method that has the ability to display the antibody diversity from individual sera sample with low cost. Immunosignaturing is a medical diagnostic test that has many applications in current medical research and in diagnosis. From a previous clinical study, patients diagnosed for lung cancer were tested for their immunosignature vs. healthy non-cancer volunteers. The pattern of reactivity against the random peptides (the ‘immunosignature’) revealed common signals in cancer patients, absent from healthy controls. My study involved the search for common amino acid motifs in the cancer-specific peptides. My search through the hundreds of ‘hits’ revealed certain motifs that were repeated more times than expected by random chance. The amino acids that were the most conserved in each set include tryptophan, aspartic acid, glutamic acid, proline, alanine, serine, and lysine. The most overall conserved amino acid observed between each set was D - aspartic acid. The motifs were short (no more than 5-6 amino acids in a row), but the total number of motifs I identified was large enough to assure significance. I utilized Excel to organize the large peptide sequence libraries, then CLUSTALW to cluster similar-sequence peptides, then GLAM2 to find common themes in groups of peptides. In so doing, I found sequences that were also present in translated cancer expression libraries (RNA) that matched my motifs, suggesting that immunosignatures can find cancer-specific antigens that can be both diagnostic and potentially therapeutic.
ContributorsShiehzadegan, Shima (Author) / Johnston, Stephen (Thesis director) / Stafford, Phillip (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2015-12
Description
This project explores the history of technology and social media, and their impact on the music industry. Social media and music culture are part of a remix culture, which encompasses new ways of recreating old content. Social media is not a new phenomenon, but has existed for centuries in various forms, dating back to ancient cultures. Music is constantly changing due to the remix culture- each new music style is created by changing what exists to fit the individual musician's style. Technological advances pushed the music industry to change, from the start to audio recording, to the digital sharing that is present in 2015, creating the musical culture as we presently know it. Due to the way that social media and music are interacting, a new platform is necessary to serve the artists in the music industry. Marketing on social media is incredibly powerful, but is ill-suited for small artists. As a result, SoundScope was developed to serve the needs of small musicians on social media. Soundscope develops a website that lets musicians use social media to it's greatest capacity and take advantage of the remix culture and the concept of virality that has developed with online communication. It uses a voting/ranking system to populate and organize it's home page, allowing listeners to find the most popular music first, and artists can get feedback based on the voting system and commenting capability. These create the community necessary for effective marketing and sharing of garage artist's music in the digital sphere.
ContributorsCash, Aimee Nicole (Author) / Silcock, Bill (Thesis director) / Dodge, Nancie (Committee member) / Walter Cronkite School of Journalism and Mass Communication (Contributor) / Barrett, The Honors College (Contributor)
Created2015-12
Description
In a dormant state, cancer cells survive chemotherapy leaving the opportunity for cancer cell relapse and metastasis ultimately leading to patient death. A novel aminoglycoside-based hydrogel ‘Amikagel’ developed in Dr. Rege’s lab serves as a platform for a 3D tumor microenvironment (3DTM) mimicking cancer cell dormancy and relapse. Six Amikagels of varying mechanical stiffness and adhesivities were synthesized and evaluated as platforms for 3DTM formation through cell viability and cell cycle arrest analyses. The impact of fetal bovine serum concentration and bovine serum albumin concentration in the media were studied for their impact on 3DTM formation. These experiments allow us to identify the best possible Amikagel formulation for 3DTM.
ContributorsGjertsen, Haley Nicole (Author) / Rege, Kaushal (Thesis director) / Grandhi, Taraka Sai Pavan (Committee member) / Chemical Engineering Program (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
This study investigates the use and perception of communications efforts among 197 animal-related and human services nonprofit organizations. Several facets of nonprofit communication such as traditional communication usage, social media adoption and usage, and the overall perception of the organizations' communications efforts were examined using a survey and Form 990 analysis. More in-depth analysis was conducted on the participating organizations' Facebook and Twitter accounts as well. After analyzing this data, the study found significant differences in how these two types of nonprofit organizations conduct their communications efforts. Animal-related organizations were much more active and saw higher levels of engagement on Facebook than human services organizations; however, there were no differences in how both types of organizations used Twitter. This study also found that human services organizations are more likely to have full-time or part-time staff members in charge of their communications, while animal-related organizations were more likely to assign this responsibility to a volunteer. These findings contribute valuable insight into how different types of nonprofit organizations are communicating with their stakeholders.
ContributorsColeman, Alexandria Elizabeth (Author) / Wu, Xu (Thesis director) / Taj, Torrie (Committee member) / Walter Cronkite School of Journalism and Mass Communication (Contributor) / School of Historical, Philosophical and Religious Studies (Contributor) / School of Community Resources and Development (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
A combined examination of the Charlie Hebdo attacks on January 7, 2015 and the November 13, 2015 attacks in Paris yields a social media movement that evolved within the 10 months between the attacks, a comparison between these terrorist attacks and those of September 11, 2001 and parallels between American First Amendment principles and France’s free expression laws.
The Charlie Hebdo attacks fueled an online debate over freedom of expression and religion, whereas the purpose of social media evolved into a humanitarian one following the November 13 attacks in Paris. This research looks into the individual evolutions of the related hashtags that surfaced in 2015, including #JeSuisCharlie (and its opposites, such as #JeNeSuisPasCharlie), #DonDuSang, #PorteOuverte and #RechercheParis, among others. Another research point was with the September 11 attacks—with the 9/11 attacks against the United States, few to no channels were available for the kind of immediate discussions and support seen after the Paris attacks. After spending time in Paris during the spring 2015 semester and researching the aftermath of the Charlie Hebdo attacks and the history of the publication, the conclusion rests on the idea that the online discussions perpetuated by both supporters and dissenters of the magazine contribute to a more robust, open democracy supported by these social media platforms.
A portion of this thesis also delves into the parallels and differences between the American First Amendment and the French free speech laws—all of which pertain to the Charlie Hebdo content and the online responses to the 2015 Paris attacks.
The interviews conducted include a French art history professor at the Sorbonne University in Paris, two creators of the “Je Suis Charlie” Facebook page, an American journalist living in Paris who covered the Charlie Hebdo attack and who was present during the November attacks, and a Muslim-American doctor in Phoenix who founded the American Islamic Forum for Democracy. The ideas surrounding free speech, the value of art such as that found within the pages of Charlie Hebdo, the media’s treatment of religion, traditional democratic freedoms and ties to social media revolutions are all components of this research thesis.
The Charlie Hebdo attacks fueled an online debate over freedom of expression and religion, whereas the purpose of social media evolved into a humanitarian one following the November 13 attacks in Paris. This research looks into the individual evolutions of the related hashtags that surfaced in 2015, including #JeSuisCharlie (and its opposites, such as #JeNeSuisPasCharlie), #DonDuSang, #PorteOuverte and #RechercheParis, among others. Another research point was with the September 11 attacks—with the 9/11 attacks against the United States, few to no channels were available for the kind of immediate discussions and support seen after the Paris attacks. After spending time in Paris during the spring 2015 semester and researching the aftermath of the Charlie Hebdo attacks and the history of the publication, the conclusion rests on the idea that the online discussions perpetuated by both supporters and dissenters of the magazine contribute to a more robust, open democracy supported by these social media platforms.
A portion of this thesis also delves into the parallels and differences between the American First Amendment and the French free speech laws—all of which pertain to the Charlie Hebdo content and the online responses to the 2015 Paris attacks.
The interviews conducted include a French art history professor at the Sorbonne University in Paris, two creators of the “Je Suis Charlie” Facebook page, an American journalist living in Paris who covered the Charlie Hebdo attack and who was present during the November attacks, and a Muslim-American doctor in Phoenix who founded the American Islamic Forum for Democracy. The ideas surrounding free speech, the value of art such as that found within the pages of Charlie Hebdo, the media’s treatment of religion, traditional democratic freedoms and ties to social media revolutions are all components of this research thesis.
ContributorsLierle, Emily Wheeler (Author) / Rodriguez, Rick (Thesis director) / Thornton, Leslie (Committee member) / Walter Cronkite School of Journalism and Mass Communication (Contributor) / School of International Letters and Cultures (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05