Matching Items (28)
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- All Subjects: Cancer
- All Subjects: Biological Sciences
- Creators: School of Molecular Sciences
- Creators: College of Liberal Arts and Sciences
- Resource Type: Text
- Status: Published
Description
Cancer rates vary between people, between cultures, and between tissue types, driven by clinically relevant distinctions in the risk factors that lead to different cancer types. Despite the importance of cancer location in human health, little is known about tissue-specific cancers in non-human animals. We can gain significant insight into how evolutionary history has shaped mechanisms of cancer suppression by examining how life history traits impact cancer susceptibility across species. Here, we perform multi-level analysis to test how species-level life history strategies are associated with differences in neoplasia prevalence, and apply this to mammary neoplasia within mammals. We propose that the same patterns of cancer prevalence that have been reported across species will be maintained at the tissue-specific level. We used a combination of factor analysis and phylogenetic regression on 13 life history traits across 90 mammalian species to determine the correlation between a life history trait and how it relates to mammary neoplasia prevalence. The factor analysis presented ways to calculate quantifiable underlying factors that contribute to covariance of entangled life history variables. A greater risk of mammary neoplasia was found to be correlated most significantly with shorter gestation length. With this analysis, a framework is provided for how different life history modalities can influence cancer vulnerability. Additionally, statistical methods developed for this project present a framework for future comparative oncology studies and have the potential for many diverse applications.
ContributorsFox, Morgan Shane (Author) / Maley, Carlo C. (Thesis director) / Boddy, Amy (Committee member) / Compton, Zachary (Committee member) / School of Mathematical and Statistical Sciences (Contributor) / School of Molecular Sciences (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
Description
The goal of this project was to design and create a genetic construct that would allow for <br/>tumor growth to be induced in the center of the wing imaginal disc of Drosophila larvae, the <br/>R85E08 domain, using a heat shock. The resulting transgene would be combined with other <br/>transgenes in a single fly that would allow for simultaneous expression of the oncogene and, in <br/>the surrounding cells, other genes of interest. This system would help establish Drosophila as a <br/>more versatile and reliable model organism for cancer research. Furthermore, pilot studies were <br/>performed, using elements of the final proposed system, to determine if tumor growth is possible <br/>in the center of the disc, which oncogene produces the best results, and if oncogene expression <br/>induced later in development causes tumor growth. Three different candidate genes were <br/>investigated: RasV12, PvrACT, and Avli.
ContributorsSt Peter, John Daniel (Author) / Harris, Rob (Thesis director) / Varsani, Arvind (Committee member) / School of Molecular Sciences (Contributor) / Department of Psychology (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
Description
Plants are essential to human life. They release oxygen into the atmosphere for us to breathe. They also provide shelter, medicine, clothing, tools, and food. For many people, the food that is on their tables and in their supermarkets isn't given much thought. Where did it come from? What part of the plant is it? How does it relate to others in the plant kingdom? How do other cultures use this plant? The most many of us know about them is that they are at the supermarket when we need them for dinner (Nabhan, 2009) (Vileisis, 2008).
ContributorsBarron, Kara (Author) / Landrum, Leslie (Thesis director) / Swanson, Tod (Committee member) / Pigg, Kathleen (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor)
Created2012-12
Description
Current studies in Multiple Myeloma suggest that patient tumors and cell lines cluster separately based on gene expression profiles. Hyperdiploid patients are also extremely underrepresented in established human myeloma cell lines (HMCLs). This suggests that the average HMCL model system does not accurately represent the average myeloma patient. To investigate this question we performed a combined CNA and SNV evolutionary comparison between four myeloma tumors and their established HMCLs (JMW-1, VP-6, KAS-6/1-KAS-6/2 and KP-6). We identified copy number changes shared between the tumors and their cell lines (mean of 74 events - 59%), those unique to patients (mean of 21.25 events - 17%), and those only in the cell lines (mean of 30.75 events \u2014 24%). A relapse sample from the JMW-1 patient showed 58% similarity to the primary diagnostic tumor. These data suggest that, on the level of copy number abnormalities, HMCLs show equal levels of evolutionary divergence as that observed within patients. By exome sequencing, patient tumors were 71% similar to their representative HMCLs, with ~12.5% and ~16.5% of SNVs unique to the tumors and HMCLs respectively. The HMCLs studied appear highly representative of the patient from which they were derived, with most differences associated with an enrichment of sub-populations present in the primary tumor. Additionally, our analysis of the KP-6 aCGH data showed that the patient's hyperdiploid karyotype was maintained in its respective HMCL. This discovery confirms the establishment and validation of a novel and potentially clinically relevant hyperdiploid HMCL that could provide a major advance in our ability to understand the pathogenesis and progression of this prominent patient population.
ContributorsBenard, Brooks Avery (Author) / Keats, Jonathan (Thesis director) / Anderson, Karen (Committee member) / Jelinek, Diane (Committee member) / School of Molecular Sciences (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
This thesis shows analyses of mixing and transport patterns associated with Hurricane Katrina as it hit the United States in August of 2005. Specifically, by applying atmospheric velocity information from the Weather Research and Forecasting System, finite-time Lyapunov exponents have been computed and the Lagrangian Coherent Structures have been identified. The chaotic dynamics of material transport induced by the hurricane are results from these structures within the flow. Boundaries of the coherent structures are highlighted by the FTLE field. Individual particle transport within the hurricane is affected by the location of these boundaries. In addition to idealized fluid particles, we also studied inertial particles which have finite size and inertia. Basing on established Maxey-Riley equations of the dynamics of particles of finite size, we obtain a reduced equation governing the position process. Using methods derived from computer graphics, we identify maximizers of the FTLE field. Following and applying these ideas, we analyze the dynamics of inertial particle transport within Hurricane Katrina, through comparison of trajectories of dierent sized particles and by pinpointing the location of the Lagrangian Coherent Structures.
ContributorsWake, Christian (Author) / Tang, Wenbo (Thesis director) / Moustaoui, Mohamed (Committee member) / Kostelich, Eric (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor)
Created2012-12
Description
Affecting over 34 million people worldwide, (0.5% of the world population) HIV/AIDS is a pandemic that is not receding its control anytime soon (Sidibe 2011). Thirty years since the chaotic emergence of fear and misunderstanding, our knowledge of the virus and its subsequent syndrome has grown exponentially, but how much of this information is really getting to the people that need it? In the corners of the Earth, where scientific knowledge rarely reaches, what can we do to stop the deadly spread of this virus? And what of the countries with a large amount of knowledge, but still a ravaging problem present in their countries? When this information is disseminated- sometimes a matter of ‘if’ in certain countries, it is primarily through unreliable sources, most of the countries examined through the media, which has a tendency to skew and misinterpret information-specially scientific. This is the information that enters the lives of people in several different countries, for example, the United States, France, China, Brazil, Uganda, and South Africa: Misunderstandings of how to protect themselves from the virus, and its effects on the body. These misunderstandings have led to millions of lives lost as myths such as showering to cure AIDS and that AIDS only infect the ‘sinners’ continue to surface throughout the globe. The Public Health threat is due to knowledge deficits, and incorrect perceived ‘knowledge’ and ‘awareness of the problem’. Here, in a six-country analysis of common misconceptions and the subsequent policies and prevalence rate, it has begun to be clear that the hardest hit areas are those with the most stigma, the most misguided policies, the most uninformed leadership, and because of this, the most mislead citizens. The biological misunderstandings of HIV/AIDS are at the root of the public health threat continuing to keep its hold in the modern world, 30 years after its documented outbreak.
ContributorsSwanson, Marissa (Author) / Verrelli, Brian (Thesis director) / Rosenberg, Michael (Committee member) / Tassone, Erica (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor)
Created2012-12
Description
This study was conducted as part of an underlying initiative to elucidate the mechanism of action of natural antibacterial clay minerals for application as therapeutic agents for difficult-to-treat infections such as methicillin-resistant Staphylococcus aureus (MRSA)-derived skin lesions and Buruli ulcer. The goal of this investigation was to determine whether exposure to the leachate of an antibacterial clay mineral, designated as CB, produced DNA double-strand breaks (DSBs) in Escherichia coli. A neutral comet assay for bacterial cells was adapted to assess DSB levels upon exposure to soluble antimicrobial compounds. Challenges involved with the adaptation process included comet visualization and data collection. To appropriately account for antimicrobial-mediated strand fragmentation, suitable control reactions comprised of exposures to water, ethanol, kanamycin, and bleomycin were developed and optimized for the assay. Bacterial exposure to CB resulted in significantly longer comet lengths compared to negative control exposures, suggesting that CB killing activity involves the induction of DNA DSBs. The results of this investigation further characterize the antimicrobial mechanisms associated with a particular clay mineral mixture. The adapted comet assay protocol described herein functions as an effective tool to assess double-strand fragmentation resulting from exposure to soluble antimicrobial compounds and to visually compare results from experimental and control reactions.
ContributorsSolanky, Dipesh (Author) / Haydel, Shelley (Thesis director) / Stout, Valerie (Committee member) / Adusumilli, Sarojini (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor)
Created2012-12
Description
Previous research has yielded an equivocal answer as to whether speaking aloud while performing intelligence tasks improves, impairs, or has no effect on performance. Some studies show that it impairs performance while others show it aids performance. In the studies in which speaking aloud has been shown to help, only children and older adults benefitted. The present study investigated whether college-aged students benefit from speaking aloud while performing a fluid intelligence test. Subjects performed a battery of working memory and intelligence tasks silently. Once they had completed each task, the participants took them again, though this time they spoke aloud while completing the tests. Results showed that subjects did insignificantly worse on the working memory tests when speaking aloud. However, subjects performed significantly better on the measures of fluid intelligence while speaking aloud as opposed to doing them silently. At an individual differences level, low working memory capacity participants benefited more from speaking aloud than the high working memory ones. Finally, we found a positive correlation between working memory scores and fluid intelligence scores, offering further evidence that the two constructs are related, yet different.
ContributorsRice, Z. Douglas (Author) / Brewer, Gene (Thesis director) / Duch, Carsten (Committee member) / Ball, Hunter (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor)
Created2012-12
Description
Spongiform Encephalopathies are a rare family of degenerative brain diseases characterized by the accumulation of plaques and formation of tiny holes in the brain tissue making it look "spongy". Spongiform Encephalopathies have a relatively short history but their origins date back to a time long before they were recognized as a disease. It was not until the 1700s that the first record of their existence was made. In 1732 a shepherd in England noticed that some sheep in his flock had become itchy and were "scraping" themselves on nearby trees and fence posts; he reported it to the agricultural authorities of the time. As the symptoms seen in his sheep progressed they also developed problems walking and began to have seizures. Eventually their neurological symptoms progressed to an unmanageable level and they died. In 1794, over 50 years later, the Board of Agriculture in the UK termed this illness in sheep "the Rubbers". In the following years while coming in and out of mention in many flocks of sheep "the Rubbers" remained a disease of minimal consequence showing negligible ability to spread among sheep and having no precedence for jumping the species barrier and affecting humans. The first mention of "the Rubbers" as Scrapie was in 1853, and it is still the designation of the disease in sheep today.
ContributorsPruniski, Brianna (Author) / Green, Monica (Thesis director) / Hurlbut, James (Committee member) / Hunter, Joel (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor)
Created2012-12
Description
Cancer is a disease that occurs in many and perhaps all multicellular organisms. Current research is looking at how different life history characteristics among species could influence cancer rates. Because somatic maintenance is an important component of a species' life history, we hypothesize the same ecological forces shaping the life history of a species should also determine its cancer susceptibility. By looking at varying life histories, potential evolutionary trends could be used to explain differing cancer rates. Life history theory could be an important framework for understanding cancer vulnerabilities with different trade-offs between life history traits and cancer defenses. Birds have diverse life history strategies that could explain differences in cancer suppression. Peto's paradox is the observation that cancer rates do not typically increase with body size and longevity despite an increased number of cell divisions over the animal's lifetime that ought to be carcinogenic. Here we show how Peto’s paradox is negatively correlated for cancer within the clade, Aves. That is, larger, long-lived birds get more cancer than smaller, short-lived birds (p=0.0001; r2= 0.024). Sexual dimorphism in both plumage color and size differ among Aves species. We hypothesized that this could lead to a difference in cancer rates due to the amount of time and energy sexual dimorphism takes away from somatic maintenance. We tested for an association between a variety of life history traits and cancer, including reproductive potential, growth rate, incubation, mating systems, and sexual dimorphism in both color and size. We found male birds get less cancer than female birds (9.8% vs. 11.1%, p=0.0058).
ContributorsDolan, Jordyn Nicole (Author) / Maley, Carlo (Thesis director) / Harris, Valerie (Committee member) / Boddy, Amy (Committee member) / School of Molecular Sciences (Contributor) / Department of Psychology (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05