Search Content

Investigating the role of SGEF (ARHGEF26) in cell survival through its interactions with BRCA1 in TMZ-resistant Glioblastoma

Description

Glioblastoma (GB) is one of the deadliest cancers and the most common form of adult primary brain tumors. SGEF (ARHGEF26) has been previously shown to be overexpressed in GB tumors, play a role in cell invasion/migration, and increase temozolomide (TMZ) resistance.[3] It was hypothesized parental LN229 cell lines with SGEF…

Glioblastoma (GB) is one of the deadliest cancers and the most common form of adult primary brain tumors. SGEF (ARHGEF26) has been previously shown to be overexpressed in GB tumors, play a role in cell invasion/migration, and increase temozolomide (TMZ) resistance.[3] It was hypothesized parental LN229 cell lines with SGEF knockdown (LN229-SGEFi) will show decreased metabolism in the MTS assay and decreased colony formation in a colony formation assay compared to parental LN229 cells after challenging the two cell lines with TMZ. For WB and co-immunoprecipitation (co-IP), parental LN229 cells with endogenous SGEF and BRCA were expected to interact and stain in the BRCA1:IP WB. LN229-SGEFi cells were expected to show very little SGEF precipitated due to shRNA targeted knockdown of SGEF. In conditions with mutations in the BRCA1 binding site (LN229-SGEFi + AdBRCAm/AdDM), SGEF expression was expected to decrease compared to parental LN229 or LN229-SGEFi cells reconstituted with WT SGEF (LN229-SGEFi + AdWT). LN229 infected with AdSGEF with a mutated nuclear localization signal (LN229-SGEFi + AdNLS12m) were expected to show BRCA and SGEF interaction since whole cell lysates were used for the co-IP. MTS data showed no significant differences in metabolism between the two cell lines at all three time points (3, 5, and 7 days). Western blot analysis was successful at imaging both SGEF and BRCA1 protein bands from whole cell lysate. The CFA showed no significant difference between cell lines after being challenged with 500uM TMZ. The co-IP immunoblot showed staining for BRCA1 and SGEF for all lysate samples, including unexpected lysates such as LN229-SGEFi, LN229-SGEFi + AdBRCAm, and LN229-SGEFi + AdDM. These results suggested either an indirect protein interaction between BRCA1 and SGEF, an additional BRCA binding site not included in the consensus, or possible detection of the translocated SGEF in knockdown cells lines since shRNA cannot enter the nucleus. Further optimization of CO-IP protocol, MTS assay, and CFA will be needed to characterize the SGEF/BRCA1 interaction and its role in cell survival.

ContributorsNabaty, Natalie Lana (Author) / Douglas, Lake (Thesis director) / Loftus, Joseph C. (Committee member) / School of Life Sciences (Contributor) / Department of Psychology (Contributor) / Barrett, The Honors College (Contributor)

Created2021-05

Heat Shock-Inducible Model to Study Tumor Environment In Drosophila

Description

The goal of this project was to design and create a genetic construct that would allow for tumor growth to be induced in the center of the wing imaginal disc of Drosophila larvae, the R85E08 domain, using a heat shock. The resulting transgene would be combined with other transgenes in…

The goal of this project was to design and create a genetic construct that would allow for tumor growth to be induced in the center of the wing imaginal disc of Drosophila larvae, the R85E08 domain, using a heat shock. The resulting transgene would be combined with other transgenes in a single fly that would allow for simultaneous expression of the oncogene and, in the surrounding cells, other genes of interest. This system would help establish Drosophila as a more versatile and reliable model organism for cancer research. Furthermore, pilot studies were performed, using elements of the final proposed system, to determine if tumor growth is possible in the center of the disc, which oncogene produces the best results, and if oncogene expression induced later in development causes tumor growth. Three different candidate genes were investigated: RasV12, PvrACT, and Avli.

ContributorsSt Peter, John Daniel (Author) / Harris, Rob (Thesis director) / Varsani, Arvind (Committee member) / School of Molecular Sciences (Contributor) / Department of Psychology (Contributor) / Barrett, The Honors College (Contributor)

Created2021-05

Effect of a Local Labor Demand Shock on Postsecondary Education Enrollment

Description

A growing number of jobs in the US require a college degree or technical education, and the wage difference between jobs requiring a high school diploma and a college education has increased to over $17,000 per year. Enrollment levels in postsecondary education have been rising for at least the past…

A growing number of jobs in the US require a college degree or technical education, and the wage difference between jobs requiring a high school diploma and a college education has increased to over $17,000 per year. Enrollment levels in postsecondary education have been rising for at least the past decade, and this paper attempts to tease out how much of the increasing enrollment is due to changes in the demand by companies for workers. A Bartik Instrument, which is a measure of local area labor demand, for each county in the US was constructed from 2007 to 2014, and using multivariate linear regression the effect of changing labor demand on local postsecondary education enrollment rates was examined. A small positive effect was found, but the effect size in relation to the total change in enrollment levels was diminutive. From the start to the end of the recession (2007 to 2010), Bartik Instrument calculated unemployment increased from 5.3% nationally to 8.2%. This level of labor demand contraction would lead to a 0.42% increase in enrollment between 2008 and 2011. The true enrollment increase over this period was 7.6%, so the model calculated 5.5% of the enrollment increase was based on the changes in labor demand.

ContributorsHerder, Daniel Steven (Author) / Dillon, Eleanor (Thesis director) / Schoellman, Todd (Committee member) / Economics Program in CLAS (Contributor) / Department of Psychology (Contributor) / Sandra Day O'Connor College of Law (Contributor) / School of Politics and Global Studies (Contributor) / Barrett, The Honors College (Contributor)

Created2016-05

Language as a Barrier to the Eurozone Being an Optimum Currency Area

Description

The European Union has increasingly integrated since World War II to the point where most European countries now share a currency and have freedom of movement for travelers and workers. This has created asymmetries in the European economy because of reports and studies that have found a low labor mobility,…

The European Union has increasingly integrated since World War II to the point where most European countries now share a currency and have freedom of movement for travelers and workers. This has created asymmetries in the European economy because of reports and studies that have found a low labor mobility, which is a requirement of a common currency area. This paper uses an econometric model and the theory of optimum currency areas to look at whether what language grouping a migrant is from affects his or her migration decision. The paper also looks at what an inflexible labor market may mean for European Central Bank policymakers and the macroeconomic outlook of the eurozone.

ContributorsHagler, Andrew Jon (Author) / Mendez, Jose (Thesis director) / Hill, John (Committee member) / Barrett, The Honors College (Contributor) / Economics Program in CLAS (Contributor) / School of Historical, Philosophical and Religious Studies (Contributor)

Created2014-05

The Euro-Zone Crisis

Description

The situation in the Euro-Zone is fluctuating daily with various efforts to curb the contagion of certain Euro-Zone member states. In the effort to focus on the greater macroeconomic and social impact of the Euro-Zone, this paper concentrates on the history of the Euro-Zone, the causes of the crisis, outlines…

The situation in the Euro-Zone is fluctuating daily with various efforts to curb the contagion of certain Euro-Zone member states. In the effort to focus on the greater macroeconomic and social impact of the Euro-Zone, this paper concentrates on the history of the Euro-Zone, the causes of the crisis, outlines potential solutions, discusses individual perspectives on the issue, and describes a prediction for the future of the Euro-Zone.

ContributorsTom, Bryan (Co-author) / Smith, Kelley (Co-author) / Mendez, Jose (Thesis director) / Datta, Manjira (Committee member) / Roberts, Nancy (Committee member) / Barrett, The Honors College (Contributor) / School of International Letters and Cultures (Contributor) / Department of Finance (Contributor) / School of Mathematical and Statistical Sciences (Contributor) / Economics Program in CLAS (Contributor) / W. P. Carey School of Business (Contributor)

Created2013-05

Investigate and characterize the means to reduce the toxicity of genetically engineered cancer therapeutic recombinant attenuated Salmonella

Description

Cancer is one of the leading causes of death in the world and represents a tremendous burden on patients, families and societies. S. Typhimurium strains are specifically attracted to compounds produced by cancer cells and could overcome the traditional therapeutic barrier. However, a major problem with using live attenuated Salmonella…

Cancer is one of the leading causes of death in the world and represents a tremendous burden on patients, families and societies. S. Typhimurium strains are specifically attracted to compounds produced by cancer cells and could overcome the traditional therapeutic barrier. However, a major problem with using live attenuated Salmonella as anti-cancer agents is their toxicity at the dose required for therapeutic efficacy, but reducing the dose results in diminished efficacy. In this project, we explored novel means to reduce the toxicity of the recombinant attenuated Salmonella by genetically engineering those virulence factors to facilitate maximal colonization of tumor tissues and reduced fitness in normal tissues. We have constructed two sets of Salmonella strains. In the first set, each targeted gene was knocked out by deletion of the gene. In the second set, the predicted promoter region of each gene was replaced with a rhamnose-regulated promoter, which will cease the synthesis of these genes in vivo, a rhamnose-free environment.

ContributorsBenson, Lee Samuel (Author) / Kong, Wei (Thesis director) / Martin, Thomas (Committee member) / Lake, Douglas (Committee member) / Barrett, The Honors College (Contributor) / Department of Psychology (Contributor) / Center for Infectious Diseases and Vaccinology (Contributor) / School of Life Sciences (Contributor)

Created2013-05

Electronic-Word-of-Mouth: A Content Analysis of Social Media Channels For Southwestern United States Luxury Hotels and Resorts

Description

Social media has quickly become a dominant tool for businesses across all sectors due to its two-way communication capabilities. Previous research has suggested that companies, particularly the hospitality and travel industry, should be engaging in authentic dialogue with its audience members, be using vibrant imagery and be monitoring and promoting…

Social media has quickly become a dominant tool for businesses across all sectors due to its two-way communication capabilities. Previous research has suggested that companies, particularly the hospitality and travel industry, should be engaging in authentic dialogue with its audience members, be using vibrant imagery and be monitoring and promoting user-generated content and electronic-word-of-mouth. These elements were observed for six luxury hotels and resorts in the Southwestern United States over the course of a month on Facebook, Twitter and TripAdvisor. In addition, three two-part electronic-questionnaires were administered to three of the six luxury hotels and resorts to determine industry perspectives on these subjects and to serve as a comparison of social media tactics in this sector. There were social media differences and similarities based on the location and size of the hotel. Facebook was comprised of 42 percent advertising and used large amounts of imagery to promote the properties. There was very little user-generated content and word-of-mouth. Twitter was comprised of 31 percent dialogue and 22 percent user-generated content. Five of the six properties responded to reviews on TripAdvisor. Three crisis responses via social media were also observed. Later research may choose to include more analytic-based research and examine other social media platforms.

ContributorsWininger, Emily Renee (Author) / Wu, Xu (Thesis director) / Ostrom, Amy (Committee member) / Barrett, The Honors College (Contributor) / Department of Psychology (Contributor) / Walter Cronkite School of Journalism and Mass Communication (Contributor) / School of Social Transformation (Contributor)

Created2014-05

Chicken, Comments, and Change: Using Chick-fil-A to Examine Facebook as an Activist Tool

Description

In 2012, Chick-fil-A president Dan Cathy's "came out" about his anti-same sex marriage views, launching an enormous negative backlash across social media networks. To counteract this, former governor Mike Huckabee called on his Facebook fans to support the company on "Chick-fil-A Appreciation Day," both on Facebook and in person. The…

In 2012, Chick-fil-A president Dan Cathy's "came out" about his anti-same sex marriage views, launching an enormous negative backlash across social media networks. To counteract this, former governor Mike Huckabee called on his Facebook fans to support the company on "Chick-fil-A Appreciation Day," both on Facebook and in person. The project examines both the backlash and Appreciation Day on social media networks. Posts on the Appreciation Day Facebook event page and similar posts on Twitter were first broken down in the framework of supportive and oppositional posts and then analyzed in further contexts. Comments on official Chick-fil-A Facebook statuses were then examined in a similar fashion. The research concludes that a strong support system both online and offline were necessary for Chick-fil-A to recover from its backlash. The controversy that ensued is ultimately a case study in the growing influence of Facebook as a tool for small-scale activism.

ContributorsKuiland, Zachary Rico (Author) / Cheong, Pauline (Thesis director) / Szeli, Eva (Committee member) / Lim, Merlyna (Committee member) / Barrett, The Honors College (Contributor) / School of Criminology and Criminal Justice (Contributor) / Department of Psychology (Contributor)

Created2013-05

Trade Liberalization and Economic Degradation

Description

This thesis looks at the theory and empirical evidence that surrounds the debate between environmentalists and economists regarding the link between trade liberalization and environmental degradation. The main points of the theory are the scale, composition, and technique effects which, when aggregated, are ambiguous as the harm or benefit of…

This thesis looks at the theory and empirical evidence that surrounds the debate between environmentalists and economists regarding the link between trade liberalization and environmental degradation. The main points of the theory are the scale, composition, and technique effects which, when aggregated, are ambiguous as the harm or benefit of trade's effect on the environment. The empirical evidence studied ranges in time periods from the early 1990s to 2011 and mainly focuses on the existence or absence of an environmental Kuznets curve for certain pollutant. However, the data still proves to be inconclusive. The debate about the possible link between trade and the environment is as important as ever, especially in regards to carbon dioxide emissions. Going forward, it is extremely important that international cooperation regarding emissions targets and abatement goals increases. Trade will prove to be an invaluable tool in this endeavor as it provides a mechanism for the spread of green technology as well as can be used as a method of environmental policy enforcement.

ContributorsCotterell, Emily Claire (Author) / Mendez, Jose (Thesis director) / McDaniel, Cara (Committee member) / School of Sustainability (Contributor) / Department of Economics (Contributor) / Department of Supply Chain Management (Contributor) / W. P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)

Created2016-05

A Time Series Analysis of the Effects of American Economic Sanctions on the Sudanese Economy

Description

This project looks at the effects of American sanctions on the Sudanese economy. The purpose of the research is to evaluate the effects of the sanctions on the GDP (based on Purchasing Power Parity) of Sudan using linear regression analysis. We used a linear model to conduct analysis that included…

This project looks at the effects of American sanctions on the Sudanese economy. The purpose of the research is to evaluate the effects of the sanctions on the GDP (based on Purchasing Power Parity) of Sudan using linear regression analysis. We used a linear model to conduct analysis that included variables such as Sudan's trading partners, distance between Sudan and said partners, the GDP of these other countries, and whether there are sanctions imposed. The data collected runs from 1980 to 2011 \u2014 the year South Sudan became independent. The results of the analysis indicate that sanctions are ineffective in achieving their purpose which is ending the human rights violations in Sudan. The findings are consistent with arguments put forth by economics for decades.

ContributorsAli, Safa (Author) / Mendez, Jose (Thesis director) / Ali, Souad T. (Committee member) / Barrett, The Honors College (Contributor) / School of Criminology and Criminal Justice (Contributor) / Department of Economics (Contributor)

Created2014-12

Filtering by