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Investigating the role of SGEF (ARHGEF26) in cell survival through its interactions with BRCA1 in TMZ-resistant Glioblastoma

Description

Glioblastoma (GB) is one of the deadliest cancers and the most common form of adult primary brain tumors. SGEF (ARHGEF26) has been previously shown to be overexpressed in GB tumors, play a role in cell invasion/migration, and increase temozolomide (TMZ) resistance.[3] It was hypothesized parental LN229 cell lines with SGEF…

Glioblastoma (GB) is one of the deadliest cancers and the most common form of adult primary brain tumors. SGEF (ARHGEF26) has been previously shown to be overexpressed in GB tumors, play a role in cell invasion/migration, and increase temozolomide (TMZ) resistance.[3] It was hypothesized parental LN229 cell lines with SGEF knockdown (LN229-SGEFi) will show decreased metabolism in the MTS assay and decreased colony formation in a colony formation assay compared to parental LN229 cells after challenging the two cell lines with TMZ. For WB and co-immunoprecipitation (co-IP), parental LN229 cells with endogenous SGEF and BRCA were expected to interact and stain in the BRCA1:IP WB. LN229-SGEFi cells were expected to show very little SGEF precipitated due to shRNA targeted knockdown of SGEF. In conditions with mutations in the BRCA1 binding site (LN229-SGEFi + AdBRCAm/AdDM), SGEF expression was expected to decrease compared to parental LN229 or LN229-SGEFi cells reconstituted with WT SGEF (LN229-SGEFi + AdWT). LN229 infected with AdSGEF with a mutated nuclear localization signal (LN229-SGEFi + AdNLS12m) were expected to show BRCA and SGEF interaction since whole cell lysates were used for the co-IP. MTS data showed no significant differences in metabolism between the two cell lines at all three time points (3, 5, and 7 days). Western blot analysis was successful at imaging both SGEF and BRCA1 protein bands from whole cell lysate. The CFA showed no significant difference between cell lines after being challenged with 500uM TMZ. The co-IP immunoblot showed staining for BRCA1 and SGEF for all lysate samples, including unexpected lysates such as LN229-SGEFi, LN229-SGEFi + AdBRCAm, and LN229-SGEFi + AdDM. These results suggested either an indirect protein interaction between BRCA1 and SGEF, an additional BRCA binding site not included in the consensus, or possible detection of the translocated SGEF in knockdown cells lines since shRNA cannot enter the nucleus. Further optimization of CO-IP protocol, MTS assay, and CFA will be needed to characterize the SGEF/BRCA1 interaction and its role in cell survival.

ContributorsNabaty, Natalie Lana (Author) / Douglas, Lake (Thesis director) / Loftus, Joseph C. (Committee member) / School of Life Sciences (Contributor) / Department of Psychology (Contributor) / Barrett, The Honors College (Contributor)

Created2021-05

Heat Shock-Inducible Model to Study Tumor Environment In Drosophila

Description

The goal of this project was to design and create a genetic construct that would allow for tumor growth to be induced in the center of the wing imaginal disc of Drosophila larvae, the R85E08 domain, using a heat shock. The resulting transgene would be combined with other transgenes in…

The goal of this project was to design and create a genetic construct that would allow for tumor growth to be induced in the center of the wing imaginal disc of Drosophila larvae, the R85E08 domain, using a heat shock. The resulting transgene would be combined with other transgenes in a single fly that would allow for simultaneous expression of the oncogene and, in the surrounding cells, other genes of interest. This system would help establish Drosophila as a more versatile and reliable model organism for cancer research. Furthermore, pilot studies were performed, using elements of the final proposed system, to determine if tumor growth is possible in the center of the disc, which oncogene produces the best results, and if oncogene expression induced later in development causes tumor growth. Three different candidate genes were investigated: RasV12, PvrACT, and Avli.

ContributorsSt Peter, John Daniel (Author) / Harris, Rob (Thesis director) / Varsani, Arvind (Committee member) / School of Molecular Sciences (Contributor) / Department of Psychology (Contributor) / Barrett, The Honors College (Contributor)

Created2021-05

Art Education For Incarcerated Youth

Description

My project is designed to provide art education to incarcerated youth in Arizona. This project will address two current issues in Arizona; the underfunding of art programs and high rates of incarceration. As of 2021, there are no state-funded art programs in Arizona. Arizona is tied with Texas for the…

My project is designed to provide art education to incarcerated youth in Arizona. This project will address two current issues in Arizona; the underfunding of art programs and high rates of incarceration. As of 2021, there are no state-funded art programs in Arizona. Arizona is tied with Texas for the eighth highest rate of incarceration in the country. In Arizona, 750 out of every 100,000 people are incarcerated. This project is an art course for incarcerated youth. The project includes a packet detailing the course content and assignment details, a class syllabus, a course flyer, and a certificate of completion. The course is intended to be taught at the Adobe Mountain School facility. The course is designed so that it can be implemented in other facilities in the future. The class will be taught by volunteers with a background in studio art, design, or art education. Each student will receive a course packet that they can use to keep track of information and assignments. Instructors will use the course packet to teach the class. The course focuses on drawing with charcoal and oil pastel, which will build a foundation in drawing skills. The course covers a twelve-week semester. The course content packet includes a week-by-week breakdown of the teaching material and project descriptions. The course consists of two main projects and preparatory work. The preparatory work includes vocabulary terms, art concepts, drawing guides, brainstorming activities, and drawing activities. The two main prompts are designed for students to explore the materials and to encourage self-reflection. The class is curated so that students can create art in a low-risk, non-judgemental environment. The course will also focus on establishing problem-solving and critical thinking skills through engaging activities.

ContributorsSheppard, Eve (Author) / Cornelia, Wells (Thesis director) / Jennifer, Nelson (Committee member) / School of Art (Contributor) / Department of Psychology (Contributor) / Barrett, The Honors College (Contributor)

Created2021-05

The Impact of Social Controls on Police Officers' Perceptions of Use of Force

Description

Police use of force has become a topic of national discussion, particularly in the wake of the deaths of Michael Brown and Eric Garner. Currently, the focus seems to be on individual officers and their individual attitudes and beliefs. Given that use of force is an individual decision it is…

Police use of force has become a topic of national discussion, particularly in the wake of the deaths of Michael Brown and Eric Garner. Currently, the focus seems to be on individual officers and their individual attitudes and beliefs. Given that use of force is an individual decision it is intuitive to think that an officer's decision to use force would be impacted by his or her attitudes and beliefs. This reasoning ignores the larger social and organizational contexts within which police officers are situated. Specifically, an officer's peer culture and department may exert control over his or her attitudes and behaviors regarding use of force. The purpose of the current study is to determine whether these larger social contexts impact an individual's perceptions regarding use of force. Using data from a nationally representative survey sample, the study finds that individual attitudes significantly predict officers' willingness to report another officer's excessive use of force. However, this relationship weakens when including measures of peer culture and departmental influence. These findings suggest that perceptions of use of force are influenced by more than just individual attitudes towards use of force. Limitations and future research suggestions are discussed.

ContributorsMorse, Stephanie Jean (Author) / Wright, Kevin (Thesis director) / Ready, Justin (Committee member) / Barrett, The Honors College (Contributor) / School of Criminology and Criminal Justice (Contributor) / Department of Psychology (Contributor)

Created2015-05

Sheldon the Shy Tortoise: A Children's Book About Shyness

Description

This creative project is a children's book entitled Sheldon the Shy Tortoise. Accompanying the story is a literature review of the research on childhood shyness. The purpose of the project is to gain a better of understanding of shyness in childhood. Topics covered in the literature review include risk factors…

This creative project is a children's book entitled Sheldon the Shy Tortoise. Accompanying the story is a literature review of the research on childhood shyness. The purpose of the project is to gain a better of understanding of shyness in childhood. Topics covered in the literature review include risk factors and causes, negative social and behavioral effects, impact on academics, and treatment options. Using this information, the children's book was written. It aims to be fun for children to read while also providing insight and encouragement into some of the problems related to being shy. The story features animal characters and a relatively simple plot so it is easily understandable by the target audience of late-preschool and early-elementary children. The main character, Sheldon the tortoise, is often physically and metaphorically "stuck in his shell". He wants to participate in social activities but is afraid to do so. Through a series of events and interactions, Sheldon starts to come out of his shell in every sense of the phrase. The book is illustrated using photographs of hand-crocheted stuffed animals representing each of the characters. By incorporating scholarly research into the writing process, children will hopefully be able to gain an understanding of their shyness and ways to help decrease it. Teachers should be able to better understand their shy students and understand some of the unique challenges of working with shy children. This creative project helps convey necessary information to children and families during a critical period of development.

ContributorsRyan, Amanda (Author) / Hansen, Cory (Thesis director) / Bernstein, Katie (Committee member) / Department of Speech and Hearing Science (Contributor) / Sanford School of Social and Family Dynamics (Contributor) / Department of Psychology (Contributor) / Barrett, The Honors College (Contributor)

Created2016-05

Investigate and characterize the means to reduce the toxicity of genetically engineered cancer therapeutic recombinant attenuated Salmonella

Description

Cancer is one of the leading causes of death in the world and represents a tremendous burden on patients, families and societies. S. Typhimurium strains are specifically attracted to compounds produced by cancer cells and could overcome the traditional therapeutic barrier. However, a major problem with using live attenuated Salmonella…

Cancer is one of the leading causes of death in the world and represents a tremendous burden on patients, families and societies. S. Typhimurium strains are specifically attracted to compounds produced by cancer cells and could overcome the traditional therapeutic barrier. However, a major problem with using live attenuated Salmonella as anti-cancer agents is their toxicity at the dose required for therapeutic efficacy, but reducing the dose results in diminished efficacy. In this project, we explored novel means to reduce the toxicity of the recombinant attenuated Salmonella by genetically engineering those virulence factors to facilitate maximal colonization of tumor tissues and reduced fitness in normal tissues. We have constructed two sets of Salmonella strains. In the first set, each targeted gene was knocked out by deletion of the gene. In the second set, the predicted promoter region of each gene was replaced with a rhamnose-regulated promoter, which will cease the synthesis of these genes in vivo, a rhamnose-free environment.

ContributorsBenson, Lee Samuel (Author) / Kong, Wei (Thesis director) / Martin, Thomas (Committee member) / Lake, Douglas (Committee member) / Barrett, The Honors College (Contributor) / Department of Psychology (Contributor) / Center for Infectious Diseases and Vaccinology (Contributor) / School of Life Sciences (Contributor)

Created2013-05

Sum sed cogitone? Can children introspect their mental states?

Description

Introspective awareness refers to direct access to one’s own internal and subjective thoughts and feelings (Wimmer & Hartl, 1991). Two theories, simulation theory and theory-theory, have been used to understand our access to our mental states. Simulation theory (Harris, 1991) involves imagining yourself in another person’s situation, reading off of…

Introspective awareness refers to direct access to one’s own internal and subjective thoughts and feelings (Wimmer & Hartl, 1991). Two theories, simulation theory and theory-theory, have been used to understand our access to our mental states. Simulation theory (Harris, 1991) involves imagining yourself in another person’s situation, reading off of your mental state, and attributing that state to the other person. Theory-theory (Gopnik, 1993) involves an interrelated body of knowledge, based on core mental-state constructs, including beliefs and desires, that may be applied to everyone—self and others (Gopnik & Wellman, 1994). Introspection is taken for granted by simulation theory, and explicitly denied by theory-theory. This study is designed to test for evidence of introspection in young children using simple perception and knowledge task. The current evidence is against introspective awareness in children because the data suggest that children cannot report their own false beliefs and they cannot report their on-going thoughts (Flavell, Green & Flavell, 1993; Gopnik & Astington, 1988). The hypothesis in this study states that children will perform better on Self tasks compared to Other tasks, which will be evidence for introspection. The Other-Perception tasks require children to calculate the other’s line of sight and determine if there is something obscuring his or her vision. The Other-Knowledge tasks require children to reason that the other’s previous looking inside a box means that he or she will know what is inside the box when it is closed. The corresponding Self tasks could be answered either by using the same reasoning for the self or by introspection to determine what it is they see and do not see, and know and do not know. Children performing better on Self tasks compared to Other tasks will be an indication of introspection. Tests included Yes/No and Forced Choice questions, which was initially to ensure that the results will not be caused by a feature of a single method of questioning. I realized belatedly, however, that Forced Choice was not a valid measure of introspection as children could introspect in both the Self and Other conditions. I also expect to replicate previous findings that reasoning about Perception is easier for children than reasoning about Knowledge.

ContributorsAamed, Mati (Author) / Fabricius, William (Thesis director) / Glenberg, Arthur (Committee member) / Kupfer, Anne (Committee member) / Department of Psychology (Contributor) / Barrett, The Honors College (Contributor) / T. Denny Sanford School of Social and Family Dynamics (Contributor)

Created2013-12

Evolutionary Social Psychology, Social Dominance Theory, and Implicit Bias in the Criminal Justice System: An Interdisciplinary Insight into Mass Incarceration

Description

The United States has become home to the largest incarcerated population in the world, containing 25% of the world's prisoners (NAACP, 2013). Within this population, young men of color appear to be severely overrepresented. This phenomenon can be better understood with the aid of a multi-disciplinary approach within the social…

The United States has become home to the largest incarcerated population in the world, containing 25% of the world's prisoners (NAACP, 2013). Within this population, young men of color appear to be severely overrepresented. This phenomenon can be better understood with the aid of a multi-disciplinary approach within the social sciences. Evolutionary theory is combined with multiple psychological and sociological perspectives, in order to more deeply understand the multi-level intersection of prejudice and discrimination against society's disadvantaged or vulnerable populations. A synthesis of the multiple theoretical angles of social dominance theory, affordance management, and life history theory is used to suggest a threat-based, attributional framework for understanding punitive decision-making and policy support. This conceptualization also considers the importance of the legal system in effecting social change. Future research within the legal arena is recommended to enable a more refined understanding of punitive ideology and implicit bias within the criminal justice system.

ContributorsLeiferman, Lindsay M (Author) / Szeli, Ãâ°va (Thesis director) / GÃÂ³mez, Alan (Committee member) / Neuberg, Steven (Committee member) / Barrett, The Honors College (Contributor) / School of Social Transformation (Contributor) / Department of Psychology (Contributor)

Created2014-05

The Effects of Unemployment on Children

Description

This research looked at the effects unemployment had on children. A searched of the previous research few studies on the effects unemployment had on children. The leading research article, or genesis of the later studies found on the topic, was the 1938 study done by Einsenburg and Lazafeld. In that…

This research looked at the effects unemployment had on children. A searched of the previous research few studies on the effects unemployment had on children. The leading research article, or genesis of the later studies found on the topic, was the 1938 study done by Einsenburg and Lazafeld. In that study, they found that children experience many negative effects from having an unemployed parent. In the current study, a total of 111 participants, (79 females and 32 males), in the study most of the volunteers came from Arizona State University, and the surrounding area. The research hypothesis (H1) was that individuals who had an unemployed parent as a child (Children/child for this study was defined being between the ages of 10-15) were more likely to be depressed, isolated, bullied, have an increase of illness, be less optimistic about the future and experience a decline in school performance than individuals whose parents were never unemployed. The current study found that having an unemployed parent led to being more depressed, isolated, optimistic, and having lower school performance and self-esteem in adolescence. Interestingly the study also found that as an adult the child of unemployed parents was more likely to be bullied as an adult. The results of this study furthered the research on the effects of unemployment had on children, and recommendations were made for future studies on the effects of parents unemployment has on children.

ContributorsBearden, Christian (Author) / Lewis, Stephen (Thesis director) / Miller, Paul (Committee member) / Lane, Jennifer (Committee member) / Barrett, The Honors College (Contributor) / Department of Psychology (Contributor) / W. P. Carey School of Business (Contributor)

Created2013-05

How Social Intelligence Correlates with and Affects Securely and Insecurely Attached Individuals

Description

The author examined the relationship between social intelligence and attachment style, specifically how attachment style affects how individuals respond to social intelligence training. Students at the Herberger Young Scholars Academy, a school for the highly gifted, completed an online social intelligence training program through the Social Intelligence Institute and were…

The author examined the relationship between social intelligence and attachment style, specifically how attachment style affects how individuals respond to social intelligence training. Students at the Herberger Young Scholars Academy, a school for the highly gifted, completed an online social intelligence training program through the Social Intelligence Institute and were assessed on a number of items. These items include the Tromso Social Intelligence Scale (TSIS), the Attachment Questionnaire for Children (AQ-C), and a daily diary measure in which they recorded and rated their social interactions day to day. All participants were found to be either securely or insecurely attached, and those that were insecurely attached were further divided into insecure anxious attachment style and insecure avoidant attachment style. It was hypothesized that those with a secure attachment style would have higher initial TSIS scores than those with an insecure attachment style. It was also hypothesized that insecurely attached individuals would benefit more from the social intelligence training program than securely attached individuals indicated by "In tune" scores from the daily diaries, and insecure avoidant individuals would benefit more from the program than insecure anxious individuals indicated by "In tune" scores from the daily diaries. None of these hypotheses were supported by the data, as there was no significant difference between the initial social intelligence scores of the three attachment styles, and none of the variables measured were found to be significant predictors of "In tune" scores. Key Words: social intelligence, social intelligence training, attachment, attachment style, children, adolescents, gifted, IQ, high IQ

ContributorsPrice, Christina Nicole (Author) / Zautra, Alex (Thesis director) / Knight, George (Committee member) / Mickelson, Kristin (Committee member) / Barrett, The Honors College (Contributor) / T. Denny Sanford School of Social and Family Dynamics (Contributor) / Department of Psychology (Contributor)

Created2014-12

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