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- All Subjects: Human gut microbiota
The human gut microbiome is a complex community of microorganisms. These microbes play an important role in host health by contributing essential compounds and acting as a barrier against pathogens. However, these communities and associated functions can be impacted by factors like disease and diet. In particular, microbial fermentation of dietary components like polysaccharides, proteins, and fats that reach the gut are being examined to better understand how these biopolymers are utilized and affect community structure. Thus, evaluating the accuracy of methods used to quantify specific macromolecules is crucial to gaining a precise understanding of how gut microbes hydrolyze those substrates. This study presents findings on the accuracy of the Megazyme RS kit (Rapid) modified for high performance liquid chromatography (HPLC) readings and the DC Protein Assay when performed on samples from complex gut media with potato starch treatments and bovine serum albumin (BSA) treatments. Overall, our data indicates that the megazyme RS kit needs further modification to detect expected starch content with the HPLC and that the DC Protein Assay is not suitable for specific protein analysis.
The ASU School of Dance presents Moving Ground, November 16-19, with works by dance faculty, graduate, and visiting artists, performed at the Dance Studio Theatre, PEBE 132.
The ASU School of Dance presents Emerging Artists I, October 24-26, with works by dance MFA candidates Sara Malan-McDonald and Holly Woodridge, performed at the Dance Studio Theatre, PEBE 132.
The ASU School of Dance presents New Dance Works 3, January 24-27, with works by dance MFA candidates C-c Braun, Janie D. Ross, and Julia Vessey, performed at the Dance Studio Theatre.
The ASU School of Dance presents Emerging Artists II, November 14-16, with works by Samantha Basting and Kristin Tovson, performed at Dance Studio Theatre, PEBE 132.
The ASU School of Dance presents Lyric Reflections, November 15-18, with works by dance faculty, undergraduates, graduates, and visiting artists, performed at Galvin Playhouse Theatre.
The ASU School of Dance presents MFA Thesis Performance, October 26, with works by dance MFA candidate Andrew Marcus, performed at Dance Studio Theatre.
The ASU School of Dance presents Transition Projects II, February 20-22, with works by dance faculty and undergrad students, performed at Dance Studio Theatre, PEBE 132.