Filtering by
- Creators: School of Life Sciences
This white paper serves as an accumulation of research to guide needle exchange program (NEP) policies in the state of Arizona to decrease the transmission of infectious diseases such as HIV and HCV.
Plant-made virus-like particles (VLPs), composed of HIV-1 Gag and deconstructed gp41 proteins, have been shown to be safe and immunogenic in mice. Here, we report the successful production of HIV-1 Gag/dgp41 VLPs in Nicotiana benthamiana, using an enhanced geminivirus-based expression vector. This novel vector results in unique expression kinetics, with peak protein accumulation and minimal necrosis achieved on day 4 post-infiltration. In comparing various purification strategies, it was determined that a 20% ammonium sulfate precipitation is an effective and efficient method for removing plant proteins and purifying the recombinant VLPs of interest. If further purification is required, this may be achieved through ultracentrifugation. VLPs are a useful platform for a variety of biomedical applications and developing the technology to efficiently produce VLPs in the plant expression system is of critical importance.
If students receive a form of sexual education, it usually occurs in their K-12 years in the United States. This education serves to prepare them for sexual activity as they transition into adulthood. The study sheds light on the importance of comprehensive and inclusive sexual education in reducing stigma and promoting healthy decision-making for all students. Our study aims to examine various factors influencing a college student's sexual health, including their sexual identity, religious affiliation, regional differences in K-12, sexual education, students' perceptions of their sexual education, and their comfort in discussing these topics with healthcare providers. A survey was conducted using responses from 200 ASU students, which collected data on their sexual behavior, and their responses were used to assess their level of sexual risk. Additionally, data was collected on topics outlined in our research questions, which explored several factors that can impact sexual health. Independent samples t-tests were conducted to analyze these correlations. The findings suggested a correlation between comprehensive sexual health education and increased positive and healthy behaviors related to sexual health. While some of our hypotheses were supported, the only statistically significant one was the impact of regional K-12 sexual health education on college students' sexual health. Overall, our study highlights the importance of implementing nationwide, comprehensive sexual education to promote healthy lifestyles and prepare adolescents with the knowledge to make informed decisions as they prepare for their transition into adulthood.