Matching Items (53)
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- All Subjects: Mental Health
- All Subjects: Molecular Biology
- Creators: School of Life Sciences
- Member of: Theses and Dissertations
Description
This project is an investigation of the gene by environment (GxE) interactions’ effect on substance use outcomes among refugee communities. Substance use disorders (SUDs) are a major public health concern, affecting individuals and communities worldwide. The etiology of SUDs is complex, involving a combination of genetic, environmental, and social factors. In recent years, there has been growing interest in the role of gene by environment interactions in the development of SUDs, particularly in vulnerable populations such as refugees. Refugee populations are exposed to a range of environmental stressors that may interact with genetic factors to increase their risk of SUDs. However, a number of studies describe a “refugee paradox,” where despite having been exposed to risk factors that can lead to SUDs, they are less likely to develop SUDs. Understanding these gene by environment interactions in refugee communities is crucial for not only understanding this phenomenon, but developing effective prevention and treatment strategies for this population. This thesis aims to investigate the gene by environment interactions underlying substance use in refugee communities and to analyze different methods for gene by environment analyses, ultimately determining which method is best suited for this population.
ContributorsEwais, Mariam (Author) / Sellner, Erin (Thesis director) / Su, Jinni (Committee member) / Barrett, The Honors College (Contributor) / School of Human Evolution & Social Change (Contributor) / Department of Psychology (Contributor) / School of Life Sciences (Contributor)
Created2023-05
Description
Santé is an event planning company that aims to address the growing need for mental health support among university students. The company's focus is on creating events that are specifically designed to help students cope with stress. Santé's events offer a variety of activities and resources that cater to students' mental and emotional needs. From outdoor walks to movie night sessions, Santé's events aim to create a safe and welcoming space for students to de-stress and connect with others. With a team of experienced event planners, Santé is dedicated to providing high-quality events that promote mental wellness and help students navigate the challenges of university life.
ContributorsRajesh, Tharun (Author) / Guardado, Jalyn (Co-author) / Loewenstein, Jacklyn (Co-author) / Byrne, Jared (Thesis director) / Patel, Manish (Committee member) / Smith, Keaton (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor)
Created2023-05
Description
This thesis focuses on the complex landscape of depression prevalence and the depression treatment disparities within vulnerable populations. By examining the underlying factors contributing to the rise in depression prevalence and understanding the challenges faced by vulnerable communities we can focus future research on the critical need for novel and equitable depression treatments.
ContributorsEsser, Eliza (Author) / Stecher, Chad (Thesis director) / Domino, Marisa (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor)
Created2023-12
Description
Hispanic/Latinx college students are at a greater risk for developing problematic alcohol use and negative mental health outcomes such as depression and anxiety because they experience contextual stressors (i.e., financial stress, academic stress, peer pressure) and cultural stressors (i.e., bicultural stress, acculturative stress, discrimination). Bicultural stress may be a risk factor for depressive, anxiety, and alcohol use disorder (AUD) symptoms. The cultural value of familism may play a protective role in Hispanic/Latinx college students. The purpose of this study was to investigate the effects of bicultural stress on depressive, anxiety, and AUD symptoms in first-year Hispanic/Latinx college students, and the role familism plays on moderating the relationship between bicultural stress and the outcomes. The sample was taken from the Pathways to College Health Study (N = 264; Female = 74.9%), which was survey administered via Qualtrics to first-year, Hispanic/Latinx college students at Arizona State University. The survey captured the participants’ levels of bicultural stress, familism, depressive, anxiety, and AUD symptoms. IBM SPSS Statistics was used for data analyses where three hierarchical regression models were run investigating the main effects and interaction effect of bicultural stress and familism. Results showed that higher levels of bicultural stress were associated with higher levels of mental health but were not associated with higher levels of AUD symptoms. Additionally, familism was not significantly associated with mental health or AUD symptoms suggesting familism may not play a substantial role in Hispanic/Latinx college students. There was no interaction found between familism and bicultural stress on the outcomes. These findings may aide in informing Hispanic/Latinx college students, universities, and clinicians on the impact bicultural stress may have on mental health outcomes.
ContributorsGhazoul, Marilyn (Author) / Su, Jinni (Thesis director) / Corbin, William (Committee member) / Cruz, Rick (Committee member) / Barrett, The Honors College (Contributor) / School of Art (Contributor) / School of Life Sciences (Contributor) / Department of Psychology (Contributor)
Created2023-12
ContributorsGhazoul, Marilyn (Author) / Su, Jinni (Thesis director) / Corbin, William (Committee member) / Cruz, Rick (Committee member) / Barrett, The Honors College (Contributor) / School of Art (Contributor) / School of Life Sciences (Contributor) / Department of Psychology (Contributor)
Created2023-12
ContributorsGhazoul, Marilyn (Author) / Su, Jinni (Thesis director) / Corbin, William (Committee member) / Cruz, Rick (Committee member) / Barrett, The Honors College (Contributor) / School of Art (Contributor) / School of Life Sciences (Contributor) / Department of Psychology (Contributor)
Created2023-12
Description
T cells, a component of the adaptive immune system, play an instrumental role in directing immune responses and direct cell killing in response to pathogens and cancers. T cells recognize and signal through the T cell receptor, a protein heterodimer on the surface of T cells. The T cell receptor is a highly variable structure formed via somatic recombination; the structure recognizes peptides presented on the surface of nucleated cells by major histocompatibility complex proteins in a specific receptor-restricted, peptide-restricted manner. This balance between T cell diversity and T cell specificity stands as a barrier to efficacious development of articificial T cell receptors capable of clearing disease. T cell receptors may be tailored to produce pathogen- or cancer-specific immune responses from autologous T cell populations. This necessitates a pipeline for amplification, cloning, and expression of antigen-specific T cell receptors. This study aims to utilize influenza-specific T cell receptor chains from healthy donor T cells to test a model for T cell receptor cloning and expression. This study utilizes Gateway recombination for high-throughput cloning into mammalian expression vectors. This study has successfully amplified and cloned T cell receptor chains from a population of influenza-specific T cells from donor cell transcripts into mammalian cell expression vectors. Additionally, CD8, a coreceptor for the T cell receptor complex, was successfully cloned and inserted into a vector for expression in mammalian cells. Sanger sequencing has confirmed sequences for influenza-specific T cell receptor chains and the CD8 chain. Future application of this project includes expression in mammalian non-T cells to test for efficacy of expression and, ultimately, expression in cytotoxic cells to create lymphocytes capable of antigen-specific recognition and cytolytic killing of cells of interest.
ContributorsVale, Nolan Richard (Author) / Anderson, Karen (Thesis director) / Blattman, Joseph (Committee member) / Department of Psychology (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
The rise in community-associated methicillin-resistant Staphylococcus aureus (MRSA) infections and the ability of the organism to develop resistance to antibiotics necessitate new treatment methods for MRSA. Geopolymers (GPs) are cheap, porous materials that have demonstrated adsorptive capabilities. In this study, GPs were investigated for their ability to adsorb whole MRSA cells and MRSA secreted proteins [culture filtrate proteins (CFPs)] as a complementary method of controlling MRSA infections. GPs have been synthesized with variable pore sizes (meso/macro scale) and further modified with stearic acid (SA) to increase surface hydrophobicity. Four GPs (SA-macroGP, macroGP, SA-mesoGP, and mesoGP) were incubated with whole cells and with CFPs to quantify GP adsorption capabilities. Following MRSA culture incubation with GPs, unbound MRSA cells were filtered and plated to determine cell counts. Following CFP incubation with GPs, unbound CFPs were separated via SDS-PAGE, stained with SYPRO Ruby, and analyzed using densitometry. Results indicate that macroGP was the most effective at adsorbing whole MRSA cells. Visual banding patterns and densitometry quantitation indicate that SA-mesoGP was the most effective at adsorbing CFP. Ultimately, GP-based products may be further developed as nonselective or selective adsorbents and integrated into fibrous materials for topical applications.
ContributorsGanser, Collin (Co-author, Co-author) / Haydel, Shelley E. (Thesis director) / Seo, Don (Committee member) / Borges, Chad (Committee member) / School of Earth and Space Exploration (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
Description
Small cell carcinoma of the ovary (SCCOHT) is a rare ovarian cancer affecting young women and characterized by mutation in SMARCA4 and silencing of SMARCA2, two tumor suppressors that function as ATPases in the SWItch/Sucrose Non-Fermentable (SWI/SNF) chromatin remodeling complex. SCCOHT patients face a 5-year survival rate of only 26%, but recently we have identified sensitivity of SCCOHT models to a natural product, triptolide. This study aims to ascertain the mechanism of action of triptolide. Previous SCCOHT epigenetic drug research has shown that some drugs reverse SMARCA2 epigenetic silencing to inhibit tumor growth, therefore it is hypothesized that triptolide acts the same and restores SWI/SNF function. Cells treated with triptolide have no change in SMARCA2 expression, suggesting that re-expression of epigenetically silenced tumor suppressor gene does not underlie its mechanism of action. Growth rates following triptolide treatment were observed in the presence and absence of SMARCA4, but no difference in sensitivity was observed. Thus, it is not likely that triptolide acts by restoring SWI/SNF. Others have observed that triptolide acts on xeroderma pigmentosa type B protein (XPB), a component of super-enhancers, which are DNA regions with high levels of transcription that regulate genes responsible for cell identity and oncogenes driving tumorigenesis. Both SCCOHT-1 and BIN67 cell lines treated with triptolide displayed lower expression of the super-enhancer associated MYC oncogene compared to untreated cells, supporting the theory that triptolide could be inhibiting super-enhancers regulating oncogenes.. A western blot confirmed reduced protein levels of RNA polymerase II and bromodomain 4 (BRD4), two essential components found at high levels at super-enhancers, in BIN67 cells treated with triptolide. ChIP-sequencing of Histone H3 Lysine-27 Acetylation (H3K27ac) marks in BIN67 and SCCOHT-1 cell lines identified super-enhancers in SCCOHT using tools CREAM and ROSE, which were mapped to neighboring genes associated genes and compared with the COSMIC database to identify oncogenes, of which the top 11 were examined by qRT-PCR to ascertain whether triptolide reduces their expression. It has been found that 6 out of 11 of the oncogenes examined (SALL4, MYC, SGK1, HIST1H3B, HMGA2, and CALR) decreased in expression when treated with triptolide. Thus, there is reason to believe that triptolide’s mechanism of action is via inhibition of super-enhancers that regulate oncogene expression.
ContributorsViloria, Nicolle Angela (Author) / Lake, Douglas (Thesis director) / Hendricks, William (Committee member) / Lang, Jessica (Committee member) / School of Life Sciences (Contributor) / School of Human Evolution & Social Change (Contributor) / School of International Letters and Cultures (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
Description
Immunology, the study of the immune system and its ability to distinguish self from non-self, is a rapidly advancing sector of molecular biology. Cancer, being host derived, provides a difficult challenge for immune cells to distinguish it from normal tissue. The historic treatment of cancer has had three main methods: radiation, chemotherapy, and surgery (1). Due to recent advancements in understanding the regulatory role of adaptive immunity against cancer, researchers have been attempting to engineer therapies to enhance patients’ immunities against their cancer. Immunotherapies, both passive and active, demonstrate potential for combating many diseases. Passive immunization provides temporary protection against a pathogen, whereas active immunization teaches the patient’s system to respond to the antigen independently, giving life-long immunity. Passive immunization, generally, is a much more expensive method of providing immunity and is commonly used in emergency situations. Anti-venom, for example, uses antibodies grown in lab to neutralize venom. Examples of active immunization are vaccines, which mimic the wild-type pathogen in a way that elicits an immune response, specifically naïve lymphocyte activation and maturation into memory lymphocytes. In terms of cancer therapy, both passive and active immunization are being tested for efficacy (2).
ContributorsMarquardt, Charles Andrew (Author) / Anderson, Karen S. (Thesis director) / Mason, Hugh S. (Committee member) / Lake, Douglas F. (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05