Glioblastoma (GB) is one of the deadliest cancers and the most common form of adult primary brain tumors. SGEF (ARHGEF26) has been previously shown to be overexpressed in GB tumors, play a role in cell invasion/migration, and increase temozolomide (TMZ) resistance.[3] It was hypothesized parental LN229 cell lines with SGEF knockdown (LN229-SGEFi) will show decreased metabolism in the MTS assay and decreased colony formation in a colony formation assay compared to parental LN229 cells after challenging the two cell lines with TMZ. For WB and co-immunoprecipitation (co-IP), parental LN229 cells with endogenous SGEF and BRCA were expected to interact and stain in the BRCA1:IP WB. LN229-SGEFi cells were expected to show very little SGEF precipitated due to shRNA targeted knockdown of SGEF. In conditions with mutations in the BRCA1 binding site (LN229-SGEFi + AdBRCAm/AdDM), SGEF expression was expected to decrease compared to parental LN229 or LN229-SGEFi cells reconstituted with WT SGEF (LN229-SGEFi + AdWT). LN229 infected with AdSGEF with a mutated nuclear localization signal (LN229-SGEFi + AdNLS12m) were expected to show BRCA and SGEF interaction since whole cell lysates were used for the co-IP. MTS data showed no significant differences in metabolism between the two cell lines at all three time points (3, 5, and 7 days). Western blot analysis was successful at imaging both SGEF and BRCA1 protein bands from whole cell lysate. The CFA showed no significant difference between cell lines after being challenged with 500uM TMZ. The co-IP immunoblot showed staining for BRCA1 and SGEF for all lysate samples, including unexpected lysates such as LN229-SGEFi, LN229-SGEFi + AdBRCAm, and LN229-SGEFi + AdDM. These results suggested either an indirect protein interaction between BRCA1 and SGEF, an additional BRCA binding site not included in the consensus, or possible detection of the translocated SGEF in knockdown cells lines since shRNA cannot enter the nucleus. Further optimization of CO-IP protocol, MTS assay, and CFA will be needed to characterize the SGEF/BRCA1 interaction and its role in cell survival.

The goal of this project was to design and create a genetic construct that would allow for <br/>tumor growth to be induced in the center of the wing imaginal disc of Drosophila larvae, the <br/>R85E08 domain, using a heat shock. The resulting transgene would be combined with other <br/>transgenes in a single fly that would allow for simultaneous expression of the oncogene and, in <br/>the surrounding cells, other genes of interest. This system would help establish Drosophila as a <br/>more versatile and reliable model organism for cancer research. Furthermore, pilot studies were <br/>performed, using elements of the final proposed system, to determine if tumor growth is possible <br/>in the center of the disc, which oncogene produces the best results, and if oncogene expression <br/>induced later in development causes tumor growth. Three different candidate genes were <br/>investigated: RasV12, PvrACT, and Avli.

The COVID-19 pandemic has forced educators since 2020 to shift from all in-person learning to virtual learning through applications like Zoom. Students are now part of a collage of faces including their teachers’ who often may be dealing with technical glitches, foreign-looking interfaces, and unintentionally disruptive students. On the other side, students may struggle to find a stable working environment as they learn from home. Distance learning has been explored well before 2020, but its necessity, given the nature of a virus that preys on in-person interaction, has forced itself to the top of relevant conversation. . The issues with distance learning in primary education have roots in long standing issues with the education system as a whole. Without greater public awareness of the woes in our education system, the status quo of declining academic success, teacher salaries, and increasing classroom sizes will continue in the future. The problems with distance learning specifically represent a much more everlasting issue that is lack of accountability and action of lawmakers who are able to make these reforms.

The purpose of this study was to bring new information to the field of education research on graduation rates and school programming. Research on graduation rates and the effects of school programs exist, however there is not an abundance of research aimed specifically at Title I high schools. The goal was to find what school characteristics might impact graduation rates in this population. The thesis focused on Title I high schools in the Phoenix Union District with a graduating 2019 class of at least 250 students. This limited the effect of variability (school size, location, socioeconomic status). To research this topic, school characteristics were selected including course rigor, mentor programs, and college prep programs, as well as specific schools. To obtain the information, multiple sources were used including the Arizona Department of Education website, school websites, and school administrators/staff. The research revealed that the effect of course rigor, college prep programs, and mentorship on graduation rates in Phoenix Union High Schools is not apparent. Further research should be conducted into other possible causes for the gaps in graduation rates between the Title I high schools in this district. Future research on ELL students and programs in the Phoenix Union district and their effectiveness or lack thereof is also recommended. The research shows that this large demographic negatively correlates with the overall graduation rates at the six schools researched.