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- Creators: School of Life Sciences
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Description
In this project I explored the relationship between Qigong and Tai Chi Easy meditative practices and cardiometabolic risk factors, specifically looking at obesity and stress. The meditative focus of Qigong and Tai Chi Easy was expected to improve cardiac vagal tone which should lead to decreases in the inflammatory effects of stress. Additionally, due to the decreases in the harmful effects of stress, we expect to see a decrease in obesity through decreases in BMI and in waist circumference.
ContributorsRameshkumar, Ramya (Author) / Larkey, Linda (Thesis director) / James, Dara (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
Description
Exploration of a mouse model (C57BL/6J) capable of demonstrating behavioral changes after adolescent social isolation that are consistent with prior findings may prove beneficial in later research. This study examined 2 proposed long-term effects of isolated housing (one mouse/cage), when compared to group housing (two mice/cage) during adolescence. Mice were placed in their respective housing conditions after weaning (PND 21) and remained in those conditions until PND 60. The same cohorts were used in both phases of the experiment. Phase 1 sought to confirm previous findings that showed increases in ethanol intake after adolescent social isolation using a 2-bottle preference Drinking-in-the-Dark (DID) design over a 4-day period (PND 64-PND 67.). Phase 2 sought to elucidate the effects present after adolescent social isolation, as measured using response inhibition capabilities demonstrated during fixed-minimum interval (FMI) trials (PND 81-PND 111). Findings in phase 1 of the experiment were non-significant, save a strong tendency for female mice in both housing conditions to drink more as a proportion of their bodyweight (g/kg). However, a trend of lower bodyweight in single housed mice did exist, which does suggest that detrimental stress was applied via the used of adolescent isolation in that housing condition. Findings in phase 2 showed little effect of adolescent social isolation on mean inter-response time (IRT) at any criterion used (FMI-0, FMI-4, FMI-6). Evaluation of mean interquartile range (IQR) of IRTs showed a significantly greater amount of variation in IRT responses within single housed mice at the highest criterion (FMI-6), and a trend in the same direction when FMI-4 and FMI-6 were tested concurrently. Taken as a whole, the findings of this experiment suggest that the effect of adolescent social isolation on ethanol intake is far less robust than the effect of sex and may be difficult to replicate in a low-power study. Additionally, adolescent social isolation may interfere with the ability of mice to show consistent accuracy during FMI tasks or a delay in recognition of FMI criterion change.
ContributorsSmith, Nathaniel B (Author) / Sanabria, Federico (Thesis director) / Olive, Foster (Committee member) / Corbin, William (Committee member) / Department of Psychology (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
Description
The transition from high school to college is associated with considerable life strain for adolescents, including higher reported levels of daily stress and negative affect (NA), and alterations in stress physiology have been linked to poor health. The purpose of this thesis was to use an ecological momentary assessment design to study associations between momentary experiences of negative affect and cortisol levels in a sample of adolescents transitioning to college. I also examined the potential moderating effects of two potential vulnerability or protective factors, alone status and perceived social support from friends. Adolescents provided salivary samples and completed paper-and-pencil diary reports of socioemotional experiences and alone status five times per day for three consecutive weekdays, as well as completed self-report questionnaires on perceived social support from friends. Within-person increases in momentary negative affect were associated with momentary cortisol reactivity. Alone status significantly moderated this association such that the association between momentary negative affect and momentary cortisol levels was only significant when individuals were with others and not when they were alone. Perceived social support from friends did not significantly moderate the within-person associations between negative affect and momentary cortisol levels. The findings add to our understanding of physiological correlates of socioemotional experiences, as well as contexts in which these associations may be exaggerated or attenuated. The findings inform our understanding of potential pathways by which physiological reactivity to socioemotional experiences may affect the health of adolescents as well as how prevention efforts could reduce potential poor health outcomes associated with heightened stress reactivity.
ContributorsKomarnisky, Sydney-Paige Milan (Author) / Doane, Leah (Thesis director) / Knight, George (Committee member) / Luecken, Linda (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor) / Department of Psychology (Contributor)
Created2014-12
Description
Major Depressive Disorder (MDD) affects over 300 million people worldwide, with the hippocampus showing decreased volume and activity in patients with MDD. The current study investigated whether a novel preclinical model of depression, unpredictable intermittent restraint (UIR), would decrease hippocampal neuronal dendritic complexity. Adult Sprague Dawley rats (24 male, 24 female) were equally divided into 4 groups: control males (CON-M), UIR males (UIR-M), control females (CON-F) and UIR females (UIR-F). UIR groups received restraint and shaking on an orbital shaker on a randomized schedule for 30 or 60 minutes/day for two to six days in a row for 26 days (21 total UIR days) before behavioral testing commenced. UIR continued and was interspersed between behavioral test days. At the end of behavioral testing, brains were processed. The behavior is published and not part of my honor’s thesis; my contribution involved quantifying and analyzing neurons in the hippocampus. Several neuronal types are found in the CA3 subregion of the hippocampus and I focused on short shaft (SS) neurons, which show different sensitivities to stress than the more common long shaft (LS) variety. Brains sections were mounted to slides and Golgi stained. SS neurons were drawn using a microscope with camera lucida attachment and quantified using the number of bifurcations and dendritic intersections as metrics for dendritic complexity in the apical and basal areas separately. The hypothesis that SS neurons in the CA3 region of the hippocampus would exhibit apical dendritic simplification in both sexes after UIR was not supported by our findings. In contrast, following UIR, SS apical dendrites were more complex in both sexes compared to controls. Although unexpected, we believe that the UIR paradigm was an effective stressor, robust enough to illicit neuronal adaptations. It appears that the time from the end of UIR to when the brain tissue was collected, or the post-stress recovery period, and/or repeated behavioral testing may have played a role in the observed increased neuronal complexity. Future studies are needed to parse out these potential effects.
ContributorsAcuna, Amanda Marie (Author) / Conrad, Cheryl (Thesis director) / Corbin, William (Committee member) / Olive, M. Foster (Committee member) / School of Life Sciences (Contributor) / Department of Psychology (Contributor) / Barrett, The Honors College (Contributor)
Created2020-12
Description
While some stress during pregnancy is normal, continuous stress during pregnancy could have negative lasting impacts on a child’s health. The type of stress that is harmful during pregnancy is “the kind that doesn’t let up” (Watson). This type of stress is chronic and is more intense than the normal stresses of everyday life. Researchers have shown that when fetuses are continuously exposed to such levels of stress, their bodies react in unhealthy ways. Given all the data showing that varying stressors experienced by pregnant women negatively affect their children’s postnatal health and development, it is important to identify the specific impacts of these stressors in order to understand how they affect the health of children.
By conducting a literature review, I have found a number of studies reporting links between a pregnant woman’s stress and the development of health issues in her child. For example, researchers of one study found that infants born to women who were depressed during pregnancy had early brain development issues and difficulty regulating emotions and stress (Hayes, et. al). In another study, researchers observed a positive association between maternal anxiety during pregnancy and asthma in offspring (Cookson, et. al). Such findings indicate the significance of the prenatal period in healthy child development. However, while we may suspect that there are some negative outcomes for children born to chronically stressed women, there was interestingly a lack of information in areas where we may expect to find effects on the child. This gap in the literature indicates that we do not fully understand the effects of stress during pregnancy, and it seems that we do not know what really seems important to know about mental health during pregnancy. Thus, the results reflect that the existing knowledge in this area is lacking, making it challenging for medical specialists to understand how they may best intervene in order to promote the healthiest pregnancies and children.
By conducting a literature review, I have found a number of studies reporting links between a pregnant woman’s stress and the development of health issues in her child. For example, researchers of one study found that infants born to women who were depressed during pregnancy had early brain development issues and difficulty regulating emotions and stress (Hayes, et. al). In another study, researchers observed a positive association between maternal anxiety during pregnancy and asthma in offspring (Cookson, et. al). Such findings indicate the significance of the prenatal period in healthy child development. However, while we may suspect that there are some negative outcomes for children born to chronically stressed women, there was interestingly a lack of information in areas where we may expect to find effects on the child. This gap in the literature indicates that we do not fully understand the effects of stress during pregnancy, and it seems that we do not know what really seems important to know about mental health during pregnancy. Thus, the results reflect that the existing knowledge in this area is lacking, making it challenging for medical specialists to understand how they may best intervene in order to promote the healthiest pregnancies and children.
ContributorsKeller, Carrie Angelique (Author) / Maienschein, Jane (Thesis director) / Abboud, Carolina (Committee member) / Historical, Philosophical & Religious Studies (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
This study examined whether cortisol changes caused by examination stress are more associated with acute psychological state or physical symptoms of stress. Participants’ salivary cortisol was assayed before and after taking a final examination, and a survey was administered to assess their psychological state for depression, tension, and fatigue, as well as the degree to which they experienced a variety of physical symptoms. Physical symptoms, tension, and depression were found to positively correlate with changes in cortisol across the examination period with depression showing the strongest correlation. No correlation was observed between fatigue and changes in cortisol during the examination period. Additionally, physical symptoms were found to positively correlate with average cortisol across the examination period while depression and fatigue were found to negatively correlate with average cortisol across the examination period. No correlation was observed between tension and average cortisol during the examination period. None of these findings were statistically significant, which suggests that no relationship exists between cortisol and acute psychological state or physical symptoms of stress; however, the study was limited by its small sample size and several potentially confounding variables, making it difficult to draw any firm conclusions.
ContributorsSchlichting, Matthew James (Author) / Nesse, Randolph (Thesis director) / Doane, Leah (Committee member) / Trumble, Benjamin (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
Abstract
This study investigates the effects of Bharatanatyam dance on stress, mood, and anxiety. I have danced Bharatanatyam since I was 8 years old, it has offered me a way to release stress and anxiety. This study provides empirical data to support the claim that Bharatanatyam has therapeutic effects that release stress and reduce anxiety. This investigation was conducted through self-reports and interviews. A Positive Affect and Negative Affect Schedule (PANAS) scale was used to determine positive and negative effects. The average positive affect during the “dance weeks” (DW) was 46.6 and the average negative affect was 12.2. During the “no dance weeks” (NDW), the average positive effect was 23.7 and the average negative affect was 31. The participant’s interview PANAS results had an average positive effect of 39.8 and an average negative effect of 12.8. Analyzing the self-report journaling highlighted a more prevalent use of positive words during the DW and a more significant use of negative words during the NDW. The Bharatanatyam dancers who were probed to enter post-performance environment for an interview also used positive words to describe Bharatanatyam dancing. In conclusion, practicing Bharatanatyam had an overall positive effect on mood, and can reduce stress and anxiety.
This study investigates the effects of Bharatanatyam dance on stress, mood, and anxiety. I have danced Bharatanatyam since I was 8 years old, it has offered me a way to release stress and anxiety. This study provides empirical data to support the claim that Bharatanatyam has therapeutic effects that release stress and reduce anxiety. This investigation was conducted through self-reports and interviews. A Positive Affect and Negative Affect Schedule (PANAS) scale was used to determine positive and negative effects. The average positive affect during the “dance weeks” (DW) was 46.6 and the average negative affect was 12.2. During the “no dance weeks” (NDW), the average positive effect was 23.7 and the average negative affect was 31. The participant’s interview PANAS results had an average positive effect of 39.8 and an average negative effect of 12.8. Analyzing the self-report journaling highlighted a more prevalent use of positive words during the DW and a more significant use of negative words during the NDW. The Bharatanatyam dancers who were probed to enter post-performance environment for an interview also used positive words to describe Bharatanatyam dancing. In conclusion, practicing Bharatanatyam had an overall positive effect on mood, and can reduce stress and anxiety.
ContributorsKothakapu, Shourya (Co-author, Co-author) / Roses-Thema, Cynthia (Thesis director) / deLusé, Stephanie (Committee member) / Mandala, Sumana (Committee member) / School of Life Sciences (Contributor) / Department of Psychology (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
Schizophrenia is a debilitating psychiatric disorder with poorly understood genetic and environmental factors. An allelic variant of complement component 4 (C4), a protein first identified in innate immune response is strongly associated with schizophrenia. In the brain, activity of C4 leads to dendritic pruning, a process that may be causal in disease progression. Environmental factors, such as early life exposure to significant stressors also associate with increased risk of schizophrenia in later life. My hypothesis is that these factors do not act independently, but rather in tandem to influence disease etiology.
This hypothesis is supported by previous studies demonstrating that stress-induced elevation of glucocorticoids increases the transcription of C4. I propose that activated glucocorticoid receptors directly increase C4 protein expression as a transcription factor activator. Additionally, I propose that activated glucocorticoid receptors inhibit the expression of the transcription factor nuclear factor-light-chain-enhancer of activated B cells (NF-κB), thereby leading to decreased expression of the C4 inhibitor CUB and Sushi multiple domains 1 (CSMD1).
Glucocorticoid receptors and C4 are richly expressed in the hippocampus, a region critical in memory consolidation, spatial, and declarative memory. I propose that stress-induced upregulation of C4 activity in the hippocampus promotes excessive synaptic pruning, contributing to specific deficits and hippocampal shrinkage seen in schizophrenia. Stress exposure during fetal development and adolescence likely acts through the proposed mechanisms to increase hippocampal C4 activity and subsequent schizophrenia risk. These mechanisms may reveal novel interactions between environmental and genetic risk factors in the etiology of schizophrenia through complement activation.
This hypothesis is supported by previous studies demonstrating that stress-induced elevation of glucocorticoids increases the transcription of C4. I propose that activated glucocorticoid receptors directly increase C4 protein expression as a transcription factor activator. Additionally, I propose that activated glucocorticoid receptors inhibit the expression of the transcription factor nuclear factor-light-chain-enhancer of activated B cells (NF-κB), thereby leading to decreased expression of the C4 inhibitor CUB and Sushi multiple domains 1 (CSMD1).
Glucocorticoid receptors and C4 are richly expressed in the hippocampus, a region critical in memory consolidation, spatial, and declarative memory. I propose that stress-induced upregulation of C4 activity in the hippocampus promotes excessive synaptic pruning, contributing to specific deficits and hippocampal shrinkage seen in schizophrenia. Stress exposure during fetal development and adolescence likely acts through the proposed mechanisms to increase hippocampal C4 activity and subsequent schizophrenia risk. These mechanisms may reveal novel interactions between environmental and genetic risk factors in the etiology of schizophrenia through complement activation.
ContributorsHoegh, Emily Marie (Author) / Orchinik, Miles (Thesis director) / Newbern, Jason (Committee member) / Talboom, Joshua (Committee member) / Department of Psychology (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2017-05
Description
Schizophrenia risk is influenced by both genetic and environmental factors. The immediate early gene early growth response 3 (Egr3), is regulated downstream of several schizophrenia risk genes and encodes a zinc-finger transcription factor protein. Previous studies from our lab indicate that Egr3 deficient (Egr3 -/-) mice exhibit schizophrenia-like phenotypes. We also discovered decreased serotonin 2a receptors (5-HT2AR) in the Egr3 -/- mice, similar to studies that reported decreased 5-HT2ARs in schizophrenia patients. We previously reported that sleep deprivation, a mild stress, causes the over expression of Egr3 and the serotonin 2a gene (Htr2a) in the cortex. To determine whether EGR3, a transcription factor, regulates Htr2a in the prefrontal cortex after sleep deprivation, Egr3 -/-and Egr3 +/+ mice were sleep deprived for eight hours. Transgenic mice were used that expressed enhanced green fluorescent protein (EGFP) under control of the Htr2a promoter via a bacterial artificial chromosome (BAC). Immunohistochemistry was performed to identify EGFP containing cells. Data analysis revealed no significant interaction between genotype and sleep deprivation in 5-HT2AR/EGFP containing cells within the prefrontal cortex. Based on the findings of this study, more data is needed to better determine the relationship between sleep deprivation and its effect on the regulation of Htr2a through in an EGR3 dependent manner.
ContributorsReznik, Derek Lee (Author) / Wilson-Rawls, Jeanne (Thesis director) / Gallitano, Amelia (Committee member) / Anderson, Karen (Committee member) / School of Sustainability (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-12
Description
The purpose of our study was to evaluate whether viewing videos of dogs had an effect on the stress response of college students. While there is strong support in the literature for demonstrating the beneficial effect of human-canine interactions on human stress indicators, there is very little to no literature on whether or not this phenomenon translates across a digital medium. We hypothesized that when exposed to a video of golden retriever puppies after a stress-inducing task, an individual would experience an increase in blood pressure recovery rate and a decline in perceived stress. In order to study this, we put together several surveys to test our participants' perceived stress, and we measured blood pressure several times in order to obtain a physiological measure of stress. Additionally, in order to produce a guaranteed stress response in our participants, we gave them 2 minutes to prepare a 4 minute video-recorded speech that they were not made aware of prior to entering the testing facility. After the speech task, the experimental group quietly viewed a pleasant 4 minute video containing imagery of dogs, while the control group sat silently for the same duration of time. During this time, the control group was asked to mentally review their performance and to focus intently on the feelings they experienced while giving their speech. Through these measures we found a significant recovery rate in systolic blood pressure and a trending difference between groups for the decline in negative affect. The data demonstrated that the experimental group had blood pressure levels that were significantly closer to their baseline levels when compared to the control group, whose blood pressure did not decline at the same rate. Additionally, the experimental group experienced a higher level of change in negative affect when asked to self-report their level of stress before the speech task and after the conditional recovery period. Interestingly, these findings can be applied to recent literature suggesting that systolic blood pressure is the most important factor of cardiac health to consider when assessing an individual for risk of heart disease or cardiac arrest. While the sample size of this study was small, the significant reduction in systolic blood pressure within the experimental group could indicate the possible efficacy of utilizing digital media containing imagery of canines as a form of therapy for systolically-hypertensive individuals as a means of managing their condition.
ContributorsDiModugno, Maria (Co-author) / Barbera, Joseph (Co-author) / Luecken, Linda (Thesis director) / Lemery-Chalfant, Kathryn (Committee member) / Department of Psychology (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2017-05