Matching Items (35)
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- All Subjects: Stress
- All Subjects: Memory
- Creators: Department of Psychology
- Creators: Bimonte-Nelson, Heather
- Member of: Theses and Dissertations
Description
Exploration of a mouse model (C57BL/6J) capable of demonstrating behavioral changes after adolescent social isolation that are consistent with prior findings may prove beneficial in later research. This study examined 2 proposed long-term effects of isolated housing (one mouse/cage), when compared to group housing (two mice/cage) during adolescence. Mice were placed in their respective housing conditions after weaning (PND 21) and remained in those conditions until PND 60. The same cohorts were used in both phases of the experiment. Phase 1 sought to confirm previous findings that showed increases in ethanol intake after adolescent social isolation using a 2-bottle preference Drinking-in-the-Dark (DID) design over a 4-day period (PND 64-PND 67.). Phase 2 sought to elucidate the effects present after adolescent social isolation, as measured using response inhibition capabilities demonstrated during fixed-minimum interval (FMI) trials (PND 81-PND 111). Findings in phase 1 of the experiment were non-significant, save a strong tendency for female mice in both housing conditions to drink more as a proportion of their bodyweight (g/kg). However, a trend of lower bodyweight in single housed mice did exist, which does suggest that detrimental stress was applied via the used of adolescent isolation in that housing condition. Findings in phase 2 showed little effect of adolescent social isolation on mean inter-response time (IRT) at any criterion used (FMI-0, FMI-4, FMI-6). Evaluation of mean interquartile range (IQR) of IRTs showed a significantly greater amount of variation in IRT responses within single housed mice at the highest criterion (FMI-6), and a trend in the same direction when FMI-4 and FMI-6 were tested concurrently. Taken as a whole, the findings of this experiment suggest that the effect of adolescent social isolation on ethanol intake is far less robust than the effect of sex and may be difficult to replicate in a low-power study. Additionally, adolescent social isolation may interfere with the ability of mice to show consistent accuracy during FMI tasks or a delay in recognition of FMI criterion change.
ContributorsSmith, Nathaniel B (Author) / Sanabria, Federico (Thesis director) / Olive, Foster (Committee member) / Corbin, William (Committee member) / Department of Psychology (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2021-05
Description
This study was conducted in order to create a brief task that more efficiently studies the memory of young and old dogs compared to previous dog radial arm mazes. The hypotheses were older dogs would perform worse than younger dogs, brief tasks with longer delays and the presence of an occluder would produce worse results, and the brief task with the longest delay period without an occluder would be most correlated to the radial arm maze. 45 dogs were tested from a previous sample that had participated in a radial arm maze experiment. The dogs were tested in their owner's homes and watched the researcher place a treat behind one of two boxes. Dogs then waited during different delay periods, of 15, 30, or 45 seconds, and with or without an occluder, which was a curtain. Then, the dog was released to see if it could still remember which box the treat was behind. The results supported all the hypotheses, except the 45-second brief task with an occluder was most correlated the radial arm maze. Additionally, the dogs that had to be excluded from the radial arm maze still had a similar range of results on the brief tasks as dogs that were able to complete the radial arm maze. These results confirm the radial arm maze is very difficult and strenuous on dogs, but the brief task is correlated and probably much more effective at studying memory without these issues. This study can help researchers perfect this simple task in order to study many dogs much quicker and collect more information on dogs' memory. Future studies could overcome limitations including dogs that were not motivated by treats or that were too old to stand up. Specific breeds could be tested or longitudinal studies could be conducted to find differences in memory over time. In the future, this can hopefully relate to human cognitive decline knowledge, as dogs show similar cognitive decline to humans, and help find treatments for cognitive diseases.
ContributorsGlomski, Marissa (Author) / Wynne, Clive (Thesis director) / Presson, Clive (Committee member) / Brewer, Gene (Committee member) / Department of Psychology (Contributor) / School of Social and Behavioral Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2015-12
Description
Women are now living longer than ever before, yet the age of spontaneous menopause has remained stable. This results in an increasing realization of the need for an effective treatment of cognitive and physiological menopausal and post-menopausal symptoms. The most common estrogen component of hormone therapy, conjugated equine estrogens (CEE; Premarin) contains many estrogens that are not endogenous to the human body, and that may or may not be detrimental to cognition (Campbell and Whitehead, 1977; Engler-Chiurazzi et al., 2011; Acosta et al., 2010). We propose the use of a novel treatment option in the form of a naturally-circulating (bioidentical) estrogen called estriol. Due to estriol’s observed positive effects on synaptic functioning and neuroprotective effects in the hippocampus (Ziehn et al., 2012; Goodman et al., 1996), a brain structure important for spatial learning and memory, estriol is promising as a hormone therapy option that may attenuate menopausal- and age- related memory decline. In the current study, we administered one of the three bioidentical estrogens (17β-Estradiol, 4.0 µg/day; Estrone, 8.0 µg/day; Estriol, 8.0 µg/day) or the vehicle polyethylene glycol by subcutaneous osmotic pump to ovariectomized Fisher-344 rats. We compared these groups to each other using a battery of spatial learning tasks, including the water radial-arm maze (WRAM), Morris water maze (MM), and delayed-match-to-sample maze (DMS). We found that all estrogens impaired performance on the WRAM compared to vehicle, while 17β-estradiol administration improved overnight forgetting performance for the MM. The estriol group showed no cognitive enhancements relative to vehicle; however, there were several factors indicating that both our estriol and estradiol doses were too high, so future studies should investigate whether lower doses of estriol may be beneficial to cognition.
ContributorsStonebarger, Gail Ashley (Author) / Bimonte-Nelson, Heather (Thesis director) / Knight, George (Committee member) / Engler-Chiurrazzi, Elizabeth (Committee member) / Barrett, The Honors College (Contributor) / Department of Psychology (Contributor)
Created2015-05
Description
The transition from high school to college is associated with considerable life strain for adolescents, including higher reported levels of daily stress and negative affect (NA), and alterations in stress physiology have been linked to poor health. The purpose of this thesis was to use an ecological momentary assessment design to study associations between momentary experiences of negative affect and cortisol levels in a sample of adolescents transitioning to college. I also examined the potential moderating effects of two potential vulnerability or protective factors, alone status and perceived social support from friends. Adolescents provided salivary samples and completed paper-and-pencil diary reports of socioemotional experiences and alone status five times per day for three consecutive weekdays, as well as completed self-report questionnaires on perceived social support from friends. Within-person increases in momentary negative affect were associated with momentary cortisol reactivity. Alone status significantly moderated this association such that the association between momentary negative affect and momentary cortisol levels was only significant when individuals were with others and not when they were alone. Perceived social support from friends did not significantly moderate the within-person associations between negative affect and momentary cortisol levels. The findings add to our understanding of physiological correlates of socioemotional experiences, as well as contexts in which these associations may be exaggerated or attenuated. The findings inform our understanding of potential pathways by which physiological reactivity to socioemotional experiences may affect the health of adolescents as well as how prevention efforts could reduce potential poor health outcomes associated with heightened stress reactivity.
ContributorsKomarnisky, Sydney-Paige Milan (Author) / Doane, Leah (Thesis director) / Knight, George (Committee member) / Luecken, Linda (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor) / Department of Psychology (Contributor)
Created2014-12
Description
This thesis aimed to discover the risks of being a high achieving student, in secondary school contexts. With the growing concern for college admission, the high achieving student has become more prevalent within society. This paper sought to gain deeper understanding into the risks and implications of attempting to achieve excellence for high achievers. Interviews with three frontline personnel at two college preparatory schools and one International Baccalaureate degree program were conducted. It was found that in the studied geographic location, peer pressure and relations, parental pressure, perfectionism, extra-curricular activities, college admission, mental health implications, and coping mechanisms are themes that are highlighted through interviews with primary staff of high achieving students. Although personnel at each of these secondary schools were clearly aware of the stress experienced by their students, a disparity remained between how certain programs managed the stress and how it negatively impacted students. College preparatory faculties appear to be more involved and current on their students' stress. This study was limited and further research should be conducted in the future that expands on this concept in various sociogeographic locations.
ContributorsClaybaugh, Anna Katherine (Author) / Scheiner Gillis, Georganne (Thesis director) / Martin, Carol (Committee member) / Wells, Cornelia (Committee member) / Barrett, The Honors College (Contributor) / School of Social Transformation (Contributor) / W. P. Carey School of Business (Contributor) / Department of Psychology (Contributor)
Created2013-05
Description
Brief memory tasks for use with pet dogs were developed using radial arm maze performance as a standard comparison measurement of memory capacity. Healthy pet dogs were first tested in a radial arm maze, where more errors made in completing the maze indicated poorer memory. These dogs were later tested with five novel memory tests, three of which utilized a treat placed behind a box with an identical distracter nearby. The treat placement was shown to each dog, and a 35 second delay, a 15 second delay with occluder, or a 15 second delay with room exit was observed before the dog could approach and find the treat. It was found that errors on the delayed match to sample (35 second delay) and occluder/object permanence (15 second delay with occluder) tasks were significantly positively correlated with the average number of errors made in the 8th trial of the radial arm maze (r =.58, p<.01** and r =.49, p<.05*, respectively) indicating that these new brief tests can reliably be used to assess memory in pet dogs.
ContributorsBoileau, Rae Nicole (Author) / Wynne, Clive (Thesis director) / Knight, George (Committee member) / Bimonte-Nelson, Heather (Committee member) / Barrett, The Honors College (Contributor) / Department of Psychology (Contributor)
Created2015-05
Description
Major Depressive Disorder (MDD) affects over 300 million people worldwide, with the hippocampus showing decreased volume and activity in patients with MDD. The current study investigated whether a novel preclinical model of depression, unpredictable intermittent restraint (UIR), would decrease hippocampal neuronal dendritic complexity. Adult Sprague Dawley rats (24 male, 24 female) were equally divided into 4 groups: control males (CON-M), UIR males (UIR-M), control females (CON-F) and UIR females (UIR-F). UIR groups received restraint and shaking on an orbital shaker on a randomized schedule for 30 or 60 minutes/day for two to six days in a row for 26 days (21 total UIR days) before behavioral testing commenced. UIR continued and was interspersed between behavioral test days. At the end of behavioral testing, brains were processed. The behavior is published and not part of my honor’s thesis; my contribution involved quantifying and analyzing neurons in the hippocampus. Several neuronal types are found in the CA3 subregion of the hippocampus and I focused on short shaft (SS) neurons, which show different sensitivities to stress than the more common long shaft (LS) variety. Brains sections were mounted to slides and Golgi stained. SS neurons were drawn using a microscope with camera lucida attachment and quantified using the number of bifurcations and dendritic intersections as metrics for dendritic complexity in the apical and basal areas separately. The hypothesis that SS neurons in the CA3 region of the hippocampus would exhibit apical dendritic simplification in both sexes after UIR was not supported by our findings. In contrast, following UIR, SS apical dendrites were more complex in both sexes compared to controls. Although unexpected, we believe that the UIR paradigm was an effective stressor, robust enough to illicit neuronal adaptations. It appears that the time from the end of UIR to when the brain tissue was collected, or the post-stress recovery period, and/or repeated behavioral testing may have played a role in the observed increased neuronal complexity. Future studies are needed to parse out these potential effects.
ContributorsAcuna, Amanda Marie (Author) / Conrad, Cheryl (Thesis director) / Corbin, William (Committee member) / Olive, M. Foster (Committee member) / School of Life Sciences (Contributor) / Department of Psychology (Contributor) / Barrett, The Honors College (Contributor)
Created2020-12
Description
Chronic stress is a risk factor for many diseases that impact the brain, including Alzheimer’s Disease. Unlike acute stress, chronic stress reduces neuronal plasticity, which can lead to neuronal remodeling and suppression. This project investigates the effect of stress on the dendritic complexity of hippocampal neurons in rats, demonstrating a methodology for procuring and analyzing these neurons. The brains of the 160 rats from the Sustained Threat and Timing (STAT) experiment were frozen. The STAT experiment investigated the effect chronic variable stress had on prospective and retrospective timing in rodents. Using a cryostat, thin coronal slices of brain tissue were placed on microscopic slides. The tissue samples were then stained using the Golgi method of silver staining. Hippocampal neurons were assessed using Sholl Analysis; the dendritic complexity of these neurons was quantified. The method of using Sholl Analysis was found to be an effective process in measuring dendritic length of hippocampal neurons.
ContributorsMiller, Amara Delaney (Author) / Sanabria, Federico (Thesis director) / Gupta, Tanya (Committee member) / School of Life Sciences (Contributor) / Department of Psychology (Contributor) / Barrett, The Honors College (Contributor)
Created2020-05
Description
Music and emotions have been studied frequently in the past as well as music and memory. However, these three items don’t have as much research grouped together. Further, this research does not also encompass culture. In my research, the aim was to examine the relationship between music, memory, emotion, and culture of gender. The hypothesis was that women had more emotions linked to music than men. We gave 416 students an animal fluency task, a letter fluency task, six cultural fluency tasks, and a cultural identity survey. We used a t-test and created a graph to analyze my data. After administering my tasks, we found that women had recalled more adjectives linked to music than men. However, there was not a statistically significant difference between the number of adjectives with emotional valence between men and women, indicating that there was no relationship between gender and emotion in regards to music. The limitations on this study included the descriptions on how to complete the task, the cultural norms of the participants, and the disparity between the number of female and male participants. In a future study, it is necessary to be more specific in what is desired from the participants and to pay close attention to shifting gender norms. Further, we would also like to see how the results from future research can impact music therapy for memory-related mood disorders.
ContributorsLevin, Allison (Author) / Brewer, Gene (Thesis director) / Cohen, Adam (Committee member) / Sanford School of Social and Family Dynamics (Contributor) / Department of Psychology (Contributor) / College of Integrative Sciences and Arts (Contributor) / School of International Letters and Cultures (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
Animal assisted activities and therapy have demonstrated efficacy but the question remains of whether the animals themselves are the mechanism of change or if other factors mediate this relationship. We investigated novelty and touch as mediators between therapy dogs and stress reduction as no other study has done both. Additionally, we were interested in whether the belief that a treatment is relaxing or simply providing a break acted as mediators. We explored these relationships using three conditions: therapy dog interaction, massage, and no-treatment control. Interacting with a therapy dog is similar to receiving a massage in each of the mediators of interest. Thus, should the therapy dogs outperform the massage in relieving stress, it suggests something there is something unique about the dogs themselves, beyond the mediators held constant for both the therapy dog and massage condition. We included the no-treatment control to determine whether treatment at all was effective in reducing stress. We tested 40 participants aged 18 to 43 years old over the course of three days. Participants were measured pre-treatment using two self-report surveys of stress, the Stress Overload Scale- Short (SOS-S) and the Stress Appraisal Measure (SAM) as well as a physiological indicator of stress, heart rate variability (HRV) measured by the Scosche Rhythm24 Waterproof Armband Heart Rate Monitor. Participants were randomly assigned to a condition for seven minutes. Afterwards, all measures were readministered. We found no significant interaction of time on condition nor any main effect of condition on any of the measures. However, we found significant main effects of time on both subscales of the SOS-S and the threat, centrality, controllable-by-others, and stressfulness subscales of the SAM. We are unable to determine whether there is a unique benefit of therapy dogs themselves but overall, the event was effective in reducing stress as reported by the participants. We recommend continued investigation of mediators in animal assisted activities and therapy.
ContributorsBryant, Gillian Varnedoe (Author) / Wynne, Clive (Thesis director) / Patock-Peckham, Julie (Committee member) / Department of Psychology (Contributor) / Barrett, The Honors College (Contributor)
Created2019-12