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- All Subjects: Communication
- Creators: Department of English
and cellular UMTS MAC protocols) across multiple unreliable communication links (such as Ethernet). The purpose is to introduce the appropriate hardware, software, and system architecture required to provide the basis for a wireless system (using a 802.11a/b/g
and cellular protocols as a model) that can scale to support thousands of users simultaneously (say in a large office building, super chain store, etc.) or in a small, but very dense communication RF region. Elements of communication between a base station and a Mobile Station will be analyzed statistically to demonstrate higher throughput, fewer collisions and lower bit error rates (BER) with the given bandwidth defined by the 802.11n wireless specification (use of MIMO channels will be evaluated). A new network nodal paradigm will be presented. Alternative link layer communication techniques will be recommended and analyzed for the affect on mobile devices. The analysis will describe how the algorithms used by state machines implemented on Mobile Stations and Wi-Fi client devices will be influenced by new base station transmission behavior. New hardware design techniques that can be used to optimize this architecture as well as hardware design principles in regard to the minimal hardware functional blocks required to support such a system design will be described. Hardware design and verification simulation techniques to prove the hardware design will accommodate an acceptable level of performance to meet the strict timing as it relates to this new system architecture.
and cellular UMTS MAC protocols) across multiple unreliable communication links using a new link layer communication model in concert with a smart antenna aperture design referred to as Vector Antenna. A vector antenna is a ‘smart’ antenna system and as any smart antenna aperture, the design inherently requires unique microwave component performance as well as Digital Signal Processing (DSP) capabilities. This performance and these capabilities are further enhanced with a patented wireless protocol stack capability.
Most protein-coding mRNAs in eukaryotes must undergo a series of processing steps so they can be exported from the nucleus and translated into protein. Cleavage and polyadenylation are vital steps in this maturation process. Improper cleavage and polyadenylation results in variation in the 3′ UTR length of genes, which is a hallmark of various human diseases. Previous data have shown that the majority of 3’UTRs of mRNAs from the nematode Caenorhabditis elegans terminate at an adenosine nucleotide, and that mutating this adenosine disrupts the cleavage reaction. It is unclear if the adenosine is included in the mature mRNA transcript or if it is cleaved off. To address this question, we are developing a novel method called the Terminal Adenosine Methylation (TAM) assay which will allow us to precisely define whether the cleavage reaction takes place upstream or downstream of this terminal adenosine. The TAM Assay utilizes the ability of the methyltransferase domain (MTD) of the human methyltransferase METTL16 to methylate the terminal adenosine of a test mRNA transcript prior to the cleavage reaction in vivo. The presence or absence of methylation at the terminal adenosine will then be identified using direct RNA sequencing. This project focuses on 1) preparing the chimeric construct that positions the MTD on the mRNA cleavage site of a test mRNA transcript, and 2) testing the functionality of this construct in vitro and developing a transgenic C. elegans strain expressing it. The TAM assay has the potential to be a valuable tool for elucidating the role of the terminal adenosine in cleavage and polyadenylation.