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- All Subjects: Biomedical Engineering
- All Subjects: Communication
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Description
As a result of the district program evaluation, a follow up on teacher perceptions of an online collaboration versus face to face collaboration approach was deemed necessary. The interviews were conducted with eight teachers from a suburban southwest K-8 public school district. After all teachers had participated in a 10 week program evaluation comparing online team teacher collaboration with face-to-face team teacher collaboration, the interview process began. One teacher from each grade level team was randomly selected to participate in the interview process. Analysis of the interview responses was inconclusive. Findings were confounded by the apparent lack of understanding of major concepts of Professional Learning Communities on the part of the participants. Assumptions about participant knowledge must be tested prior to investigations of the influence of either face to face or online format as delivery modes.
ContributorsTucker, Pamela K (Author) / McCoy, Kathleen (Thesis advisor) / Gehrke, Rebecca (Committee member) / Ham, Timothy (Committee member) / Arizona State University (Publisher)
Created2013
Description
Gene manipulation techniques, such as RNA interference (RNAi), offer a powerful method for elucidating gene function and discovery of novel therapeutic targets in a high-throughput fashion. In addition, RNAi is rapidly being adopted for treatment of neurological disorders, such as Alzheimer's disease (AD), Parkinson's disease, etc. However, a major challenge in both of the aforementioned applications is the efficient delivery of siRNA molecules, plasmids or transcription factors to primary cells such as neurons. A majority of the current non-viral techniques, including chemical transfection, bulk electroporation and sonoporation fail to deliver with adequate efficiencies and the required spatial and temporal control. In this study, a novel optically transparent biochip is presented that can (a) transfect populations of primary and secondary cells in 2D culture (b) readily scale to realize high-throughput transfections using microscale electroporation and (c) transfect targeted cells in culture with spatial and temporal control. In this study, delivery of genetic payloads of different sizes and molecular characteristics, such as GFP plasmids and siRNA molecules, to precisely targeted locations in primary hippocampal and HeLa cell cultures is demonstrated. In addition to spatio-temporally controlled transfection, the biochip also allowed simultaneous assessment of a) electrical activity of neurons, b) specific proteins using fluorescent immunohistochemistry, and c) sub-cellular structures. Functional silencing of GAPDH in HeLa cells using siRNA demonstrated a 52% reduction in the GAPDH levels. In situ assessment of actin filaments post electroporation indicated a sustained disruption in actin filaments in electroporated cells for up to two hours. Assessment of neural spike activity pre- and post-electroporation indicated a varying response to electroporation. The microarray based nature of the biochip enables multiple independent experiments on the same culture, thereby decreasing culture-to-culture variability, increasing experimental throughput and allowing cell-cell interaction studies. Further development of this technology will provide a cost-effective platform for performing high-throughput genetic screens.
ContributorsPatel, Chetan (Author) / Muthuswamy, Jitendran (Thesis advisor) / Helms Tillery, Stephen (Committee member) / Jain, Tilak (Committee member) / Caplan, Michael (Committee member) / Vernon, Brent (Committee member) / Arizona State University (Publisher)
Created2012
Description
The use of electromyography (EMG) signals to characterize muscle fatigue has been widely accepted. Initial work on characterizing muscle fatigue during isometric contractions demonstrated that its frequency decreases while its amplitude increases with the onset of fatigue. More recent work concentrated on developing techniques to characterize dynamic contractions for use in clinical and training applications. Studies demonstrated that as fatigue progresses, the EMG signal undergoes a shift in frequency, and different physiological mechanisms on the possible cause of the shift were considered. Time-frequency processing, using the Wigner distribution or spectrogram, is one of the techniques used to estimate the instantaneous mean frequency and instantaneous median frequency of the EMG signal using a variety of techniques. However, these time-frequency methods suffer either from cross-term interference when processing signals with multiple components or time-frequency resolution due to the use of windowing. This study proposes the use of the matching pursuit decomposition (MPD) with a Gaussian dictionary to process EMG signals produced during both isometric and dynamic contractions. In particular, the MPD obtains unique time-frequency features that represent the EMG signal time-frequency dependence without suffering from cross-terms or loss in time-frequency resolution. As the MPD does not depend on an analysis window like the spectrogram, it is more robust in applying the timefrequency features to identify the spectral time-variation of the EGM signal.
ContributorsAustin, Hiroko (Author) / Papandreou-Suppappola, Antonia (Thesis advisor) / Kovvali, Narayan (Committee member) / Muthuswamy, Jitendran (Committee member) / Arizona State University (Publisher)
Created2012
Description
Sensitivity is a fundamental challenge for in vivo molecular magnetic resonance imaging (MRI). Here, I improve the sensitivity of metal nanoparticle contrast agents by strategically incorporating pure and doped metal oxides in the nanoparticle core, forming a soluble, monodisperse, contrast agent with adjustable T2 or T1 relaxivity (r2 or r1). I first developed a simplified technique to incorporate iron oxides in apoferritin to form "magnetoferritin" for nM-level detection with T2- and T2* weighting. I then explored whether the crystal could be chemically modified to form a particle with high r1. I first adsorbed Mn2+ ions to metal binding sites in the apoferritin pores. The strategic placement of metal ions near sites of water exchange and within the crystal oxide enhance r1, suggesting a mechanism for increasing relaxivity in porous nanoparticle agents. However, the Mn2+ addition was only possible when the particle was simultaneously filled with an iron oxide, resulting in a particle with a high r1 but also a high r2 and making them undetectable with conventional T1-weighting techniques. To solve this problem and decrease the particle r2 for more sensitive detection, I chemically doped the nanoparticles with tungsten to form a disordered W-Fe oxide composite in the apoferritin core. This configuration formed a particle with a r1 of 4,870mM-1s-1 and r2 of 9,076mM-1s-1. These relaxivities allowed the detection of concentrations ranging from 20nM - 400nM in vivo, both passively injected and targeted to the kidney glomerulus. I further developed an MRI acquisition technique to distinguish particles based on r2/r1, and show that three nanoparticles of similar size can be distinguished in vitro and in vivo with MRI. This work forms the basis for a new, highly flexible inorganic approach to design nanoparticle contrast agents for molecular MRI.
ContributorsClavijo Jordan, Maria Veronica (Author) / Bennett, Kevin M (Thesis advisor) / Kodibagkar, Vikram (Committee member) / Sherry, A Dean (Committee member) / Wang, Xiao (Committee member) / Yarger, Jeffery (Committee member) / Arizona State University (Publisher)
Created2012
Description
Performance is a public speech act that can present the experience of difference and generate relations across lines of difference. In personal narrative performance, performers do not just tell stories, the stories they tell are strategic hailings that call attention to discourses that produce the conditions of their exclusion and form intimate relations in public. Personal narrative performance renders the private public. Performers take to the stage, the space of the public, to offer their stories, their bodies, and their relations to audiences for collective consideration. In turn, the act of performance generates further relations: among performers and audiences, and between performance and discourse. This study analyzes these two layers of relation in performance through looking at the ways neoliberalism and performance interanimate one another. Through looking at three sites of neoliberal relationality--same-sex marriage, family, and immigration and multiculturalism, it asks questions of how performers narrate and represent non-normative experiences within neoliberalism, the historical and cultural context through which they are living and narrating. In order to understand the cultural work, the resistive and relational potential, of the relations that occur in and through personal narrative performance, we also need to understand the political, cultural, and historical conditions under which narratives in performance are produced. My argument is that in and through performance intimacy is queered: it takes the private--the stuff of the personal presented as aesthetic communication--and renders that private very public. In public and through relations, performance can raise awareness and shift consciousness, reify orders of relation or generate alternate imaginaries. This is to say that a lot of different types of work are done in performance, and although performance is often seen as resistance, under the weight of neoliberalism, it is important to tend to what arguments performances are making and how in turn that shapes the relations that occur in the site of performance. Queer intimacy offers a way of engaging performance, an analytic that considers the text of performance as well as the relational context among performers and audiences, and turns back on larger cultural questions of belonging. The potential of performance, of the concept of queer intimacy, provides a lens to read performance, to tend to the conditions that give rise to and inform performance in the current historical moment. It brings together the critical impulse of intercultural communication and cultural studies with performance studies. From a critical cultural perspective, it tends to the structural in performance, and through performance emphasizes the lived experience as narrated and embodied as and through communication. Coupled with the impulses of queer theory, queer intimacy offers both resisting normativity and imagining beyond it. To consider queer intimacy in performance is not only to recognize that relations are made possible, but to tend closely to the belongings we are making.
ContributorsPérez, Kimberlee (Author) / Corey, Frederick C. (Thesis advisor) / Brouwer, Daniel C (Committee member) / Honegger, Gitta (Committee member) / Langellier, Kristin M (Committee member) / Arizona State University (Publisher)
Created2014
Description
Advances in implantable MEMS technology has made possible adaptive micro-robotic implants that can track and record from single neurons in the brain. Development of autonomous neural interfaces opens up exciting possibilities of micro-robots performing standard electrophysiological techniques that would previously take researchers several hundred hours to train and achieve the desired skill level. It would result in more reliable and adaptive neural interfaces that could record optimal neural activity 24/7 with high fidelity signals, high yield and increased throughput. The main contribution here is validating adaptive strategies to overcome challenges in autonomous navigation of microelectrodes inside the brain. The following issues pose significant challenges as brain tissue is both functionally and structurally dynamic: a) time varying mechanical properties of the brain tissue-microelectrode interface due to the hyperelastic, viscoelastic nature of brain tissue b) non-stationarities in the neural signal caused by mechanical and physiological events in the interface and c) the lack of visual feedback of microelectrode position in brain tissue. A closed loop control algorithm is proposed here for autonomous navigation of microelectrodes in brain tissue while optimizing the signal-to-noise ratio of multi-unit neural recordings. The algorithm incorporates a quantitative understanding of constitutive mechanical properties of soft viscoelastic tissue like the brain and is guided by models that predict stresses developed in brain tissue during movement of the microelectrode. An optimal movement strategy is developed that achieves precise positioning of microelectrodes in the brain by minimizing the stresses developed in the surrounding tissue during navigation and maximizing the speed of movement. Results of testing the closed-loop control paradigm in short-term rodent experiments validated that it was possible to achieve a consistently high quality SNR throughout the duration of the experiment. At the systems level, new generation of MEMS actuators for movable microelectrode array are characterized and the MEMS device operation parameters are optimized for improved performance and reliability. Further, recommendations for packaging to minimize the form factor of the implant; design of device mounting and implantation techniques of MEMS microelectrode array to enhance the longevity of the implant are also included in a top-down approach to achieve a reliable brain interface.
ContributorsAnand, Sindhu (Author) / Muthuswamy, Jitendran (Thesis advisor) / Tillery, Stephen H (Committee member) / Buneo, Christopher (Committee member) / Abbas, James (Committee member) / Tsakalis, Konstantinos (Committee member) / Arizona State University (Publisher)
Created2013
Description
Family caregivers are a quickly growing population in American society and are potentially vulnerable to a number of risks to well-being. High stress and little support can combine to cause difficulties in personal and professional relationships, physical health, and emotional health. Siblings are, however, a possible source of protection for the at-risk caregiver. This study examines the relational and health outcomes of gratitude exchange between caregivers and their siblings as they attend to the issue of caring for aging parents. Dyadic data was collected through an online survey and was analyzed using a series of Actor-Partner Interdependence Models. Intimacy and care conflict both closely relate to gratitude exchange, but the most significant variable influencing gratitude was role. Specifically, caregivers are neither experiencing nor expressing gratitude on the same level as their siblings. Expressed gratitude did not relate strongly or consistently to well-being variables, though it did relate to diminished negative affect. Implications for theory, the caregiver, the sibling, the elder, the practitioner, and the researcher are addressed in the discussion.
ContributorsAmaro, Lauren M (Author) / Miller, Katherine I. (Thesis advisor) / Alberts, Janet K. (Thesis advisor) / Updegraff, Kimberly (Committee member) / Arizona State University (Publisher)
Created2014
Description
The basal ganglia are four sub-cortical nuclei associated with motor control and reward learning. They are part of numerous larger mostly segregated loops where the basal ganglia receive inputs from specific regions of cortex. Converging on these inputs are dopaminergic neurons that alter their firing based on received and/or predicted rewarding outcomes of a behavior. The basal ganglia's output feeds through the thalamus back to the areas of the cortex where the loop originated. Understanding the dynamic interactions between the various parts of these loops is critical to understanding the basal ganglia's role in motor control and reward based learning. This work developed several experimental techniques that can be applied to further study basal ganglia function. The first technique used micro-volume injections of low concentration muscimol to decrease the firing rates of recorded neurons in a limited area of cortex in rats. Afterwards, an artificial cerebrospinal fluid flush was injected to rapidly eliminate the muscimol's effects. This technique was able to contain the effects of muscimol to approximately a 1 mm radius volume and limited the duration of the drug effect to less than one hour. This technique could be used to temporarily perturb a small portion of the loops involving the basal ganglia and then observe how these effects propagate in other connected regions. The second part applied self-organizing maps (SOM) to find temporal patterns in neural firing rate that are independent of behavior. The distribution of detected patterns frequency on these maps can then be used to determine if changes in neural activity are occurring over time. The final technique focused on the role of the basal ganglia in reward learning. A new conditioning technique was created to increase the occurrence of selected patterns of neural activity without utilizing any external reward or behavior. A pattern of neural activity in the cortex of rats was selected using an SOM. The pattern was then reinforced by being paired with electrical stimulation of the medial forebrain bundle triggering dopamine release in the basal ganglia. Ultimately, this technique proved unsuccessful possibly due to poor selection of the patterns being reinforced.
ContributorsBaldwin, Nathan Aaron (Author) / Helms Tillery, Stephen I (Thesis advisor) / Castaneda, Edward (Committee member) / Buneo, Christopher A (Committee member) / Muthuswamy, Jitendran (Committee member) / Si, Jennie (Committee member) / Arizona State University (Publisher)
Created2014
Description
The purpose of this paper is to introduce a new method of dividing wireless communication (such as the 802.11a/b/g
and cellular UMTS MAC protocols) across multiple unreliable communication links (such as Ethernet). The purpose is to introduce the appropriate hardware, software, and system architecture required to provide the basis for a wireless system (using a 802.11a/b/g
and cellular protocols as a model) that can scale to support thousands of users simultaneously (say in a large office building, super chain store, etc.) or in a small, but very dense communication RF region. Elements of communication between a base station and a Mobile Station will be analyzed statistically to demonstrate higher throughput, fewer collisions and lower bit error rates (BER) with the given bandwidth defined by the 802.11n wireless specification (use of MIMO channels will be evaluated). A new network nodal paradigm will be presented. Alternative link layer communication techniques will be recommended and analyzed for the affect on mobile devices. The analysis will describe how the algorithms used by state machines implemented on Mobile Stations and Wi-Fi client devices will be influenced by new base station transmission behavior. New hardware design techniques that can be used to optimize this architecture as well as hardware design principles in regard to the minimal hardware functional blocks required to support such a system design will be described. Hardware design and verification simulation techniques to prove the hardware design will accommodate an acceptable level of performance to meet the strict timing as it relates to this new system architecture.
and cellular UMTS MAC protocols) across multiple unreliable communication links (such as Ethernet). The purpose is to introduce the appropriate hardware, software, and system architecture required to provide the basis for a wireless system (using a 802.11a/b/g
and cellular protocols as a model) that can scale to support thousands of users simultaneously (say in a large office building, super chain store, etc.) or in a small, but very dense communication RF region. Elements of communication between a base station and a Mobile Station will be analyzed statistically to demonstrate higher throughput, fewer collisions and lower bit error rates (BER) with the given bandwidth defined by the 802.11n wireless specification (use of MIMO channels will be evaluated). A new network nodal paradigm will be presented. Alternative link layer communication techniques will be recommended and analyzed for the affect on mobile devices. The analysis will describe how the algorithms used by state machines implemented on Mobile Stations and Wi-Fi client devices will be influenced by new base station transmission behavior. New hardware design techniques that can be used to optimize this architecture as well as hardware design principles in regard to the minimal hardware functional blocks required to support such a system design will be described. Hardware design and verification simulation techniques to prove the hardware design will accommodate an acceptable level of performance to meet the strict timing as it relates to this new system architecture.
ContributorsJames, Frank (Author) / Reisslein, Martin (Thesis advisor) / Ying, Lei (Committee member) / Zhang, Yanchao (Committee member) / Arizona State University (Publisher)
Created2014
Description
The &ldquoMormon; Colonies” in Chihuahua, northern Mexico, boast a sizable population of women originally from the United States who have immigrated to these small Mexican towns. This ethnographic study of the immigrant women in the area focuses on questions of citizenship and belonging, and bolsters the scholarship on U.S. American immigrants in Mexico. Using data from 15 unstructured interviews, the women&rsquos; experiences of migration provide a portrait of U.S. American immigrants in a Mexican religious community. Analysis of this data using grounded theory has revealed that these U.S. American women have created a third social space for themselves, to a large degree retaining their original culture, language, and political loyalty. Their stories contribute to the literature on transnational migration, providing an account of the way migrants of privilege interact with their society of settlement.
ContributorsNielsen, Vanessa (Author) / Mean, Lindsey (Thesis advisor) / Téllez, Michelle (Committee member) / Gruber, Diane (Committee member) / Arizona State University (Publisher)
Created2013