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- Genre: Masters Thesis
- Member of: Theses and Dissertations
- Resource Type: Text
- Status: Published
Description
Specificity and affinity towards a given ligand/epitope limit target-specific delivery. Companies can spend between $500 million to $2 billion attempting to discover a new drug or therapy; a significant portion of this expense funds high-throughput screening to find the most successful target-specific compound available. A more recent addition to discovering highly specific targets is the application of phage display utilizing single chain variable fragment antibodies (scFv). The aim of this research was to employ phage display to identify pathologies related to traumatic brain injury (TBI), particularly astrogliosis. A unique biopanning method against viable astrocyte cultures activated with TGF-β achieved this aim. Four scFv clones of interest showed varying relative affinities toward astrocytes. One of those four showed the ability to identify reactive astroctyes over basal astrocytes through max signal readings, while another showed a statistical significance in max signal reading toward basal astrocytes. Future studies will include further affinity characterization assays. This work contributes to the development of targeting therapeutics and diagnostics for TBI.
ContributorsMarsh, William (Author) / Stabenfeldt, Sarah (Thesis advisor) / Caplan, Michael (Committee member) / Sierks, Michael (Committee member) / Arizona State University (Publisher)
Created2013
Description
In order to adapt to a new culture and new language, children of immigrant families are faced daily with the responsibility of being the intermediaries between the family and the host culture through their language proficiency (Weisskirch & Alva, 2002). This thesis looks into the experiences of English-Spanish bilingual children as they bridge the gap between the family and the non-Spanish speaking community through their interpreting/translating skills. With an emphasis on children of Mexican-origin, the goal is to further understand and illuminate how these children manage this communication in an adult society, their feelings and thoughts about their experiences, and the child's perceptions about the influence that this experience may or may not have on their future. A sample of seventeen children agreed to participate in a semi-structured face-to-face interview to share their experiences. The data from these interviews were analyzed using a thematic analysis approach (Braun & Clarke, 2006). A priori themes of circumstantial bilingual and adaptive parentification were the initial focus of the research while being open to emerging themes. The children's accounts of their experiences indicated primarily that the Mexican-origin values of familism and respeto (respect) were a significant influence on them when they interpreted/translated for their family. With these traditional cultural values and norms as the groundwork, the sub-themes of normalcy and stress emerged as supportive elements of the circumstantial bilingual experience. Furthermore, the theme of adaptive parentification and the sub-themes of choice, expectation/responsibility to assist, and equality to parents offered further insight on how adaptive parentification can result as the roles of these children change. There was an emergent theme, identity negotiation, which increases our understanding of what the circumstantial bilingual child encounters as the attempt is made to negotiate his identity as an individual who has to mediate language between two opposing cultures. Due to the language brokering responsibility that are bestowed upon these children, it is concluded that communicative support by the parents is a necessary component of the parent-child relationship in order to nurture and develop these children as they negotiate and create their identity to become the successful leaders of tomorrow.
ContributorsCayetano, Catalina (Author) / Mean, Lindsey (Thesis advisor) / Waldron, Vincent (Committee member) / Gaffney, Cynthia (Committee member) / Arizona State University (Publisher)
Created2013
Description
Alzheimer's Disease (AD) is the sixth leading cause of death in the United States and the most common form of dementia. Its cause remains unknown, but it is known to involve two hallmark pathologies: Amyloid Beta plaques and neurofibrillary tangles (NFTs). Several proteins have been implicated in the formation of neurofibrillary tangles, including Tau and S100B. S100B is a dimeric protein that is typically found bound to Ca(II) or Zn(II). These experiments relate to the involvement of S100B in Alzheimer's Disease-related processes and the results suggest that future research of S100B is warranted. Zn(II)-S100B was found to increase the rate at which tau assembled into paired helical filaments, as well as affect the rate at which tubulin polymerized into microtubules and the morphology of SH-SY5Y neuroblastoma cells after 72 hours of incubation. Zn(II)-S100B also increased the firing rate of hippocampal neurons after 36 hours of incubation. Together, these results suggest several possibilities: Zn(II)-S100B may be a key part of the formation of paired helical filaments (PHFs) that subsequently form NFTs. Zn(II)-S100B may also be competing with tau to bind tubulin, which could lead to an instability of microtubules and subsequent cell death. This finding aligns with the neurodegeneration that is commonly seen in AD and which could be a result of this microtubule instability. Ultimately, these results suggest that S100B is likely involved in several AD-related processes, and if the goal is to find an efficient and effective therapeutic target for AD, the relationship between S100B, particularly Zn(II)-S100B, and tau needs to be further studied.
ContributorsNaegele, Hayley (Author) / Mcgregor, Wade C (Thesis advisor) / Baluch, Debra (Committee member) / Francisco, Wilson (Committee member) / Arizona State University (Publisher)
Created2013
Description
Law enforcement, schools and universities, health service agencies, as well as social service agencies, each acquire information from individuals that receive their services. That information gets recorded into the respective application system of each organization. The information, however, gets recorded only in the context of each service rendered and within each system used to record it. Information that is recorded by the police department for one individual is entirely different from the information that is recorded by the hospital for that same individual. What if all the organizations used the same system to record information? What if all the organizations followed the same protocols to record information as well as access it? The goal of this research was to analyze a system that allows for all organizations within a community to share information with each other. Technically, this system is feasible. However, public opinion says sharing personal information is unethical, and Federal regulation says it is unlawful. To accomplish an information-sharing system of this type, both regulation and public opinion need to be addressed.
ContributorsPullin, Britton Scott (Author) / Schildgen, Thomas (Thesis advisor) / Prewitt, Deborah (Committee member) / Ralston, Laurel (Committee member) / Arizona State University (Publisher)
Created2012
Description
Dietary protein is known to increase postprandial thermogenesis more so than carbohydrates or fats, probably related to the fact that amino acids have no immediate form of storage in the body and can become toxic if not readily incorporated into body tissues or excreted. It is also well documented that subjects report greater satiety on high- versus low-protein diets and that subject compliance tends to be greater on high-protein diets, thus contributing to their popularity. What is not as well known is how a high-protein diet affects resting metabolic rate over time, and what is even less well known is if resting metabolic rate changes significantly when a person consuming an omnivorous diet suddenly adopts a vegetarian one. This pilot study sought to determine whether subjects adopting a vegetarian diet would report decreased satiety or demonstrate a decreased metabolic rate due to a change in protein intake and possible increase in carbohydrates. Further, this study sought to validate a new device called the SenseWear Armband (SWA) to determine if it might be sensitive enough to detect subtle changes in metabolic rate related to diet. Subjects were tested twice on all variables, at baseline and post-test. Independent and related samples tests revealed no significant differences between or within groups for any variable at any time point in the study. The SWA had a strong positive correlation to the Oxycon Mobile metabolic cart but due to a lack of change in metabolic rate, its sensitivity was undetermined. These data do not support the theory that adopting a vegetarian diet results in a long-term change in metabolic rate.
ContributorsMoore, Amy (Author) / Johnston, Carol (Thesis advisor) / Appel, Christy (Thesis advisor) / Gaesser, Glenn (Committee member) / Arizona State University (Publisher)
Created2012
Description
The &ldquoMormon; Colonies” in Chihuahua, northern Mexico, boast a sizable population of women originally from the United States who have immigrated to these small Mexican towns. This ethnographic study of the immigrant women in the area focuses on questions of citizenship and belonging, and bolsters the scholarship on U.S. American immigrants in Mexico. Using data from 15 unstructured interviews, the women&rsquos; experiences of migration provide a portrait of U.S. American immigrants in a Mexican religious community. Analysis of this data using grounded theory has revealed that these U.S. American women have created a third social space for themselves, to a large degree retaining their original culture, language, and political loyalty. Their stories contribute to the literature on transnational migration, providing an account of the way migrants of privilege interact with their society of settlement.
ContributorsNielsen, Vanessa (Author) / Mean, Lindsey (Thesis advisor) / Téllez, Michelle (Committee member) / Gruber, Diane (Committee member) / Arizona State University (Publisher)
Created2013
Description
The aim of this study is to test the feasibility of building a storytelling system for older adults to tell and share their life stories based on email. It is measured by the level of participation and people's acceptance of the system. The central goals were to empower people over 60 years old by providing a platform for them to share their wonderful life experience and perspectives on life and lead social services into the digital age by bridging traditional roundtable interaction and modern digital communication. A prototype was built to test the level of participation of the system and follow-up interviews were conducted in order to deeply understand people's acceptance. Content analysis was used to analyze the stories to ascertain what common themes were present. Key design considerations and key factors that affect the feasibility of storytelling system were discussed. This research expands on current research and implementation of Internet-based storytelling system and shed light on the future of combining storytelling with older adults' existing Internet knowledge. Key findings of this research are :(1) Frequency of reminiscence trigger and the number of active participants affect the level of participation collectively. Frequency is considered to be a key determinant. High frequency indicates high level of participation. (2) Categories of topics do not affect the level of participation significantly but serve as key attractions that enhance people's acceptance of the system. (3) Older adults highly accept and get involved in the new email storytelling system. This storytelling program helps them recall their memories and have a profound effect on their own introspection.
ContributorsLi, Yuanyi (Author) / Giard, Jacques R (Thesis advisor) / Margolis, Eric (Committee member) / Larkin, Kyle (Committee member) / Arizona State University (Publisher)
Created2014
Description
The economic crisis in 2008 triggered a global financial shockwave that left many wondering about the origins of the crisis. Similarly, in the early twentieth century, Wall Street faced catastrophic losses that set the stage for the Great Depression, which resulted in a decade of economic depression, leaving millions of people out of work. Using discourse analysis to understand how economic crisis is framed through the mainstream press, this research project analyzed the stock market crash of 1929-1932 and the mortgage-backed financial crisis of 2007-2009 through the lens of two mainstream publications, The New York Times and The Wall Street Journal. Comparative analysis focused on explanations for the causes of the crises, attributions of blame, culprits, and proposed solutions emerging in news coverage of the 1929 panic and the 2007-2009 financial crises. Mainstream media accounts of the 2007-2009 crisis are then compared with `alternative media' accounts of crisis causes, culprits, and solutions. These comparative analyses are contextualized historically within economic paradigms of thought, beginning with the classical economists led by Adam Smith and transitioning to the Chicago School.
ContributorsPrice, Eun (Author) / Nadesan, Majia (Thesis advisor) / Gruber, Diane (Committee member) / Ramsey, Ramsey E (Committee member) / Arizona State University (Publisher)
Created2013
Description
The goal of the present study was to test for links between experiences of red flags in initial interactions with romantic partners and later styles of relational conflict. Red flags are negative qualities or traits displayed by a potential romantic partner that trigger warnings in singles of possible future negative experiences if a romantic relationship develops. I focused on five red flags (displayed a lack of interest, evidenced narcissistic-like behavior, was too sexual, too possessive, or drank too much) and three conflict styles (mutual constructive style, demand/withdraw style, mutual avoidance style) in the current study. The 155 unmarried male undergraduates and the 504 unmarried female undergraduates were asked to refer back to their most recent dating partner when completing these relationship measures. The red flag measures, therefore, were measured using a retrospective approach. After controlling for whether the singles were still in their relationships and the sex of the participants, regression analyses revealed that recalling that one's partner showed a lack of interest in initial interactions were significantly and positively associated with a demand/withdraw and mutual avoidant conflict style. Results also showed that recalling that one's partner was too possessive in initial interactions was significantly and negatively associated with a mutual constructive conflict style, and positively related to partner demand/I withdraw and mutual avoidant conflict styles. Finally, recalling that their partner drank too much in a first interaction was negatively linked to later reports of engaging in a mutually constructive conflict style. This study provides insight into an area of research that has not been previously studied.
ContributorsPorter, Cami (Author) / Christopher, F (Thesis advisor) / Iida, Masumi (Committee member) / Guerrero, Laura (Committee member) / Arizona State University (Publisher)
Created2014
Description
Voice and other circuit switched services in a LTE deployment can be based on a Circuit Switched Fall Back mechanism or on the upcoming Voice Over LTE option. Voice Over LTE option can be used with its SIP based signaling to route voice calls and other circuit switched services over the LTE's packet switched core. The main issue that is faced though is the validation of this approach before the deployment over commercial network. The test strategy devised as a result of this work will be able to visit corner scenarios and error sensitive services, so that signaling involved can be verified to ensure a robust deployment of the Voice Over LTE network. Signaling test strategy is based on the observations made during a simulated Voice Over LTE call inside the lab in a controlled environment. Emergency services offered are carefully studied to devise a robust test strategy to make sure that any service failure is avoided. Other area were the service is routed via different protocol stack layer than it normally is in a legacy circuit switched core are identified and brought into the scope of the test strategy.
ContributorsThotton Veettil, Vinayak (Author) / Reisslein, Martin (Thesis advisor) / Ying, Lei (Committee member) / McGarry, Michael (Committee member) / Arizona State University (Publisher)
Created2014