Matching Items (7)
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- All Subjects: Antibodies
- All Subjects: Fashion
- All Subjects: happiness
- Status: Published
Description
This project aims to address the current protocol regarding the diagnosis and treatment of traumatic brain injury (TBI) in medical industries around the world. Although there are various methods used to qualitatively determine if TBI has occurred to a patient, this study attempts to aid in the creation of a system for quantitative measurement of TBI and its relative magnitude. Through a method of artificial evolution/selection called phage display, an antibody that binds highly specifically to a post-TBI upregulated brain chondroitin sulfate proteoglycan called neurocan has been identified. As TG1 Escheria Coli bacteria were infected with KM13 helper phage and M13 filamentous phage in conjunction, monovalent display of antibody fragments (ScFv) was performed. The ScFv bind directly to the neurocan and from screening, phage that produced ScFv's with higher affinity and specificity to neurocan were separated and purified. Future research aims to improve the ScFv characteristics through increased screening toward neurocan. The identification of a highly specific antibody could lead to improved targeting of neurocan post-TBI in-vivo, aiding researchers in quantitatively defining TBI by visualizing its magnitude.
ContributorsSeelig, Timothy Scott (Author) / Stabenfeldt, Sarah (Thesis director) / Ankeny, Casey (Committee member) / Barrett, The Honors College (Contributor) / Harrington Bioengineering Program (Contributor)
Created2015-05
Description
A chimeric, humanized monoclonal antibody that recognizes a highly conserved fusion loop found on flaviviruses was constructed with a geminiviral replicon and transiently expressed in Nicotiana benthamiana plants through Agrobacterium tumefaciens infiltration. Characterization and expression studies were then conducted to confirm correct assembly of the antibody. Once the antibody was purified, an ELISA was conducted to validate that the antibody was able to bind to the flavivirus fusion loop.
ContributorsPardhe, Mary (Author) / Mason, Hugh (Thesis director) / Chen, Qiang (Committee member) / Mor, Tsafrir (Committee member) / School of Life Sciences (Contributor) / Department of Information Systems (Contributor) / W.P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
Regardless of religious beliefs, all one has left when they leave this Earth is the meaning the individual has derived from building connections and relationships with other people. I propose whether or not one is able to reach his or her potential and the quality of life that follows is based inherently in the following question: How will you manifest your truth into reality, thus becoming the best at your profession or craft, and spreading your gifts to help the lives of others? The answer to this question, and ultimately the key to leading the best life available is in the equation: vulnerability through playfulness and connection instigates happiness, which results in spreading positivity and facilitating the ability to make fearless decisions that guides one's life as an artist or profession of one's choosing, leading one to their highest potential. This theory applies to everyone and does not discriminate. This is the beginning of a life-long exploration and discovery with these elements as I further my work as an artist, and I will explore these themes from my exposure to the Balinese people and culture, our theatre workshops and experiences there, and through additional inspiration and research. The workshop and clowning in Bali training helped form the theory I have proposed, and further introduced a different approach to acting that I utilize in my own work that incorporates taksu: an achievable state of being that is unanimously understood by the Balinese that influences and shapes how they approach life, work, and each other that I discuss more extensively in my work. I will continue to inquire about how one finds space for imagination and pursuit of taksu without traveling, although traveling is an amazing way to broaden one's scope of perspective. The combination of elements discussed and taksu help create a humanity that is more in touch with inner peace and happiness as a result of spreading both positivity and a positive mindset to others. This in turn, influences and shapes performance and how we approach the craft of acting, while creating emotional truth and integrity within one's craft.
ContributorsSwim, Hannah Grace (Author) / Espinosa, Micha (Thesis director) / de la Garza, Amira (Committee member) / School of Film, Dance and Theatre (Contributor) / W.P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
Streetwear is a category of fashion that has evolved from being a niche style of dress influenced by American counter cultures, into a formidable industry. Over time, Streetwear has become a culture in itself whose members are critical of inauthentic brands and commercialization. Streetwear brands have the need to be perceived as authentic, exclusive, and being connected to celebrity to survive in the industry long-term. Additionally, as the industry grows larger, the marketplace has become increasingly saturated. This can be attributed to how easy it is to create a fledgling streetwear brand due to advances in garment production technology and streetwear's roots in low-cost clothing. Streetwear brands need to differentiate themselves in an effective manner to stand out to consumers in the saturated streetwear market. Primary research conducted via a survey administered to streetwear consumers was done to learn more about the effectiveness of popular tactics and marketplace events streetwear brands use to differentiate themselves. Secondary research was done in literature on the topics of creativity and energized differentiation. Research by Gerzema and Lebar presented in their book The Brand Bubble on the topic of energized differentiation is reviewed and applied to streetwear brands. This thesis will discuss the key elements brands need to have to be successful in the streetwear industry. Along with this, it will also look at the effectiveness of specific tactics bands can execute to effectively differentiate themselves in the saturated industry and the important role creativity plays in their success.
ContributorsGriblin, Austin W. (Author) / Ostrom, Amy (Thesis director) / Samper, Adriana (Committee member) / Department of Marketing (Contributor) / Herberger Institute for Design and the Arts (Contributor) / W.P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
The purpose of this thesis is to study the happiest countries in the world and see what factors, laws, and values they have in common. I will be focusing on the Scandinavian countries, as they rank highest according to the World Happiness Report. First, I will research these countries to learn more about their political, economic, educational, legal, and social landscapes. Then I will interview individuals from these countries on their views as well as values and thoughts on their country. Finally, I will compare and analyze this information to come to a conclusion to see if there are similar factors that allow these countries to be ranked so highly in the department of happiness. After reading this the reader can take away ideas of how to improve their happiness as well as new perspectives on other countries.
ContributorsFrasure, Laura S. (Author) / Moore, James (Thesis director) / Siroky, David (Committee member) / W.P. Carey School of Business (Contributor) / Department of Information Systems (Contributor) / Department of Management and Entrepreneurship (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
Monoclonal antibody therapy focuses on engineering immune cells to target specific peptide sequences indicative of disease. An impediment in the continued advancement of this market is the lack of an efficient, inexpensive means of characterization that can be broadly applied to any antibody while still providing high-density data. Many characterization methods address an antibody's affinity for its cognate sequence but overlook other important aspects of binding behavior such as off-target binding interactions. The purpose of this study is to demonstrate how the binding intensity between an antibody and a library of random-sequence peptides, otherwise known as an immunosignature, can be evaluated to determine antibody specificity and polyreactivity. A total of 24 commercially available monoclonal antibodies were assayed on 125K and 330K peptide microarrays and analyzed using a motif clustering program to predict candidate epitopes within each antigen sequence. The results support the further development of immunosignaturing as an antibody characterization tool that is relevant to both therapeutic and non-therapeutic antibodies.
ContributorsDai, Jennifer T. (Author) / Stafford, Phillip (Thesis director) / Diehnelt, Chris (Committee member) / School of Life Sciences (Contributor) / W.P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
The following report addresses sustainable supply chain management (SSCM) and its application in the fashion industry. The purpose is to draw conclusions on why companies implement sustainability into their processes, and how these sustainable monitoring practices contribute to operational, competitive and financial advantages. This report contains various methods of analysis. Research derived from numerous scholarly articles on measurement methods, theories and governance structures will be discussed to develop a background on the current status of SSCM in the fashion industry, including the notable strengths and weaknesses. To understand the depth of practices involved in managing a sustainable supply chain, four leading companies within the industry will be analyzed using their annual sustainability reports. Based on this analysis, it can be concluded that sustainable practices are abundantly present in today's leading fashion companies, each having different mindsets motivating their sustainable actions. With this conclusion, it's also important to acknowledge that there's far more progress to be made in terms of sustainable development on a company and industry level, in order to make a lasting impact.
ContributorsRezzonico, Jordan Nicole (Author) / Dooley, Kevin (Thesis director) / Wiedmer, Robert (Committee member) / W.P. Carey School of Business (Contributor) / Department of Supply Chain Management (Contributor) / Department of Management and Entrepreneurship (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05