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- All Subjects: Neuroscience
- Creators: Gallitano, Amelia
Environmental and genetic factors influence schizophrenia risk. Individuals who have direct family members with schizophrenia have a much higher incidence. Also, acute stress or life crisis may precede the onset of the disease. This study aims to understand the effects of environment on genes related to schizophrenia risk. It investigates the impact of sleep deprivation as an acute environmental stressor on the expression of Htr2a in mice, a gene that codes for the serotonin 2A receptor (5-HT2AR). HTR2A is associated with schizophrenia risk through genetic association studies and expression is decreased in post-mortem studies of patients with the disease. Furthermore, sleep deprivation as a stressor in human trials has been shown to increase the binding capacity of 5-HT2AR. We hypothesize that sleep deprivation will increase the number of cells expressing Htr2a in the mouse anterior prefrontal cortex when compared to controls. Sleep deprived that mice express EGFP under control of the Htr2a promoter displayed anteroposterior gradients of expression across sagittal sections, with concentrations seen most densely within the prefrontal cortex as well as the anterior pretectal nucleus, thalamic nucleus, as well as the cingulate gyrus. Htr2a-EGFP expression was most densely visualized in cortical layer V and VI pyramidal neurons within the lateral prefrontal cortex of coronal sections. Furthermore, the medial prefrontal cortex contained significantly cells expressing Htr2a¬-EGFP than the lateral prefrontal cortex. Ultimately, the hypothesis was not supported and sleep deprivation did not result in more ¬Htr2a-EGFP expressing cells compared to basal levels. However, expressing cells appeared visibly brighter in sleep-deprived animals when compared to controls, indicating that the amount of intracellular Htr2a-GFP expression may be higher. This study provides strong visual representations of expression gradients following sleep deprivation as an acute stressor and paves the way for future studies regarding 5H-T2AR’s role in schizophrenia.
Supply & Demand, the phrase speaks to the tango between college graduates seeking employment & employers seeking talent. Recruiters desire candidates with employability skills to lead, but report significant skill gaps among applicants. This thesis aims to (1) define the skills gap evidenced by employers, (2) determine students’ career preparedness, and (3) identify strategies to bridge the gap among undergraduate students as they prepare to join the workforce. Qualtrics, an experience management, and survey platform, was used to reach and collect information from nearly 1,200 students in order to quantitatively assess their career development skills and needs. As part of this thesis, I have partnered with the T.W. Lewis Center for Personal Development, a center of Barrett, The Honors College at Arizona State University in an effort to test and provide effective solutions to bridge the employability skills gap. Through this collaboration, we have constructed a Career Development Workshop Series for students using the data collected from students. The workshop was built to teach students about professional skill topics that they desired to learn about, but could not find on or off-campus. The Lewis Center Career Development Workshop is a 5-part series with topics ranging from negotiation and job interviewing to strength-building. In each workshop, an expert is selected as the guest speaker to share their experience and insights with students as they prepare for their career journeys. Guest speakers include CEOs, entrepreneurs, business executives, and more. The series is intended to deepen students’ business acumen, so they can enter the workforce with a sustainable advantage and ultimately supported the professional and personal growth of over 100 students. The series serves as an example of ways our university can improve its career development offerings to students. In an increasingly competitive labor market, the research collected and solutions presented are designed to empower students in their careers.