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Social isolation in early childhood can have life-long effects on social behaviors and development. Cerebellar crus I has additionally been linked to social behaviors through forebrain pathways. In this study, we hypothesized that social isolation of mice from postnatal day 21 (P21) until p35 would result in impaired social behaviors. Additionally, we hypothesized that gq DREADD injections into crus I, to increase levels of cerebellar stimulation, at the start of the isolation period would counteract the effects of isolation, leading to mice who displayed normal social behaviors. Social behavior at P35 was tested using the 3-Chamber Task, a well-established model, and SLEAP deep-learning software was used to obtain quantifiable data. We found no difference in social behaviors between socially raised and isolated mice. However, gq DREADD mice displayed greater levels of social interaction and exploration than either socially raised mice or isolated mice. This research carries implications for possible therapeutic interventions for groups prone to social isolation, such as those with developmental disabilities, minority groups, the elderly, and prison populations.
Many studies indicate a positive relationship between fundamentalism and sexual and racial prejudice. Many of these studies use the Religious Fundamentalism Scale (RFS), the Attitudes Towards Homosexuals Scale (ATHS) and the Manitoba Scale. However, there appears to be overlap between RFS and both ATHS and the Manitoba Scale, unaddressed by the literature. This study looked at possible overlaps between RFS and ATHS and between RFS and the Manitoba Scale that could inflate the correlation statistic of fundamentalism and sexual and racial prejudice. The Intratextual Fundamentalism Scale (IFS), a study without authoritarian or apparent prejudice-overlapping items, was also tested for overlap. Results showed two-factor structures—namely fundamentalism and prejudice—with only two items loading to the opposite factor. However, there were many near-zero item loadings. The discussion suggests ways to change these items to increase factor loadings and to change overall measures construct validity. The correlations between fundamentalism and sexual prejudice were not significant before modifying the measures and were small and negative after modifying (modifying measures means removing all crossloaded and near-zero loaded items). The modified fundamentalism and sexual prejudice measures correlations do not follow the literature. This may be due to the sample including sexual orientation minorities and a majority of atheist, agnostic, or ‘nothing in particular’ affiliations. The correlations between fundamentalism and racial prejudice were medium and positive before modifying and were small and positive after modifying. This falls in line with the literature of small and medium positive correlation statistics.