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- All Subjects: energy
- Creators: Arizona State University
Description
A low cost expander, combustor device that takes compressed air, adds thermal energy and then expands the gas to drive an electrical generator is to be designed by modifying an existing reciprocating spark ignition engine. The engine used is the 6.5 hp Briggs and Stratton series 122600 engine. Compressed air that is stored in a tank at a particular pressure will be introduced during the compression stage of the engine cycle to reduce pump work. In the modified design the intake and exhaust valve timings are modified to achieve this process. The time required to fill the combustion chamber with compressed air to the storage pressure immediately before spark and the state of the air with respect to crank angle is modeled numerically using a crank step energy and mass balance model. The results are used to complete the engine cycle analysis based on air standard assumptions and air to fuel ratio of 15 for gasoline. It is found that at the baseline storage conditions (280 psi, 70OF) the modified engine does not meet the imposed constraints of staying below the maximum pressure of the unmodified engine. A new storage pressure of 235 psi is recommended. This only provides a 7.7% increase in thermal efficiency for the same work output. The modification of this engine for this low efficiency gain is not recommended.
ContributorsJoy, Lijin (Author) / Trimble, Steve (Thesis advisor) / Davidson, Joseph (Committee member) / Phelan, Patrick (Committee member) / Arizona State University (Publisher)
Created2011
Description
Passive cooling designs & technologies offer great promise to lower energy use in buildings. Though the working principles of these designs and technologies are well understood, simplified tools to quantitatively evaluate their performance are lacking. Cooling by night ventilation, which is the topic of this research, is one of the well known passive cooling technologies. The building's thermal mass can be cooled at night by ventilating the inside of the space with the relatively lower outdoor air temperatures, thereby maintaining lower indoor temperatures during the warmer daytime period. Numerous studies, both experimental and theoretical, have been performed and have shown the effectiveness of the method to significantly reduce air conditioning loads or improve comfort levels in those climates where the night time ambient air temperature drops below that of the indoor air. The impact of widespread adoption of night ventilation cooling can be substantial, given the large fraction of energy consumed by air conditioning of buildings (about 12-13% of the total electricity use in U.S. buildings). Night ventilation is relatively easy to implement with minimal design changes to existing buildings. Contemporary mathematical models to evaluate the performance of night ventilation are embedded in detailed whole building simulation tools which require a certain amount of expertise and is a time consuming approach. This research proposes a methodology incorporating two models, Heat Transfer model and Thermal Network model, to evaluate the effectiveness of night ventilation. This methodology is easier to use and the run time to evaluate the results is faster. Both these models are approximations of thermal coupling between thermal mass and night ventilation in buildings. These models are modifications of existing approaches meant to model dynamic thermal response in buildings subject to natural ventilation. Effectiveness of night ventilation was quantified by a parameter called the Discomfort Reduction Factor (DRF) which is the index of reduction of occupant discomfort levels during the day time from night ventilation. Daily and Monthly DRFs are calculated for two climate zones and three building heat capacities. It is verified that night ventilation is effective in seasons and regions when day temperatures are between 30 oC and 36 oC and night temperatures are below 20 oC. The accuracy of these models may be lower than using a detailed simulation program but the loss in accuracy in using these tools more than compensates for the insights provided and better transparency in the analysis approach and results obtained.
ContributorsEndurthy, Akhilesh Reddy (Author) / Reddy, T Agami (Thesis advisor) / Phelan, Patrick (Committee member) / Addison, Marlin (Committee member) / Arizona State University (Publisher)
Created2011
Description
The cyanobacterium Synechocystis sp. PCC 6803 performs oxygenic photosynthesis. Light energy conversion in photosynthesis takes place in photosystem I (PSI) and photosystem II (PSII) that contain chlorophyll, which absorbs light energy that is utilized as a driving force for photosynthesis. However, excess light energy may lead to formation of reactive oxygen species that cause damage to photosynthetic complexes, which subsequently need repair or replacement. To gain insight in the degradation/biogenesis dynamics of the photosystems, the lifetimes of photosynthetic proteins and chlorophyll were determined by a combined stable-isotope (15N) and mass spectrometry method. The lifetimes of PSII and PSI proteins ranged from 1-33 and 30-75 hours, respectively. Interestingly, chlorophyll had longer lifetimes than the chlorophyll-binding proteins in these photosystems. Therefore, photosynthetic proteins turn over and are replaced independently from each other, and chlorophyll is recycled from the damaged chlorophyll-binding proteins. In Synechocystis, there are five small Cab-like proteins (SCPs: ScpA-E) that share chlorophyll a/b-binding motifs with LHC proteins in plants. SCPs appear to transiently bind chlorophyll and to regulate chlorophyll biosynthesis. In this study, the association of ScpB, ScpC, and ScpD with damaged and repaired PSII was demonstrated. Moreover, in a mutant lacking SCPs, most PSII protein lifetimes were unaffected but the lifetime of chlorophyll was decreased, and one of the nascent PSII complexes was missing. SCPs appear to bind PSII chlorophyll while PSII is repaired, and SCPs stabilize nascent PSII complexes. Furthermore, aminolevulinic acid biosynthesis, an early step of chlorophyll biosynthesis, was impaired in the absence of SCPs, so that the amount of chlorophyll in the cells was reduced. Finally, a deletion mutation was introduced into the sll1906 gene, encoding a member of the putative bacteriochlorophyll delivery (BCD) protein family. The Sll1906 sequence contains possible chlorophyll-binding sites, and its homolog in purple bacteria functions in proper assembly of light-harvesting complexes. However, the sll1906 deletion did not affect chlorophyll degradation/biosynthesis and photosystem assembly. Other (parallel) pathways may exist that may fully compensate for the lack of Sll1906. This study has highlighted the dynamics of photosynthetic complexes in their biogenesis and turnover and the coordination between synthesis of chlorophyll and photosynthetic proteins.
ContributorsYao, Cheng I Daniel (Author) / Vermaas, Wim (Thesis advisor) / Fromme, Petra (Committee member) / Roberson, Robert (Committee member) / Webber, Andrew (Committee member) / Arizona State University (Publisher)
Created2011
Description
Arizona has an abundant solar resource and technologically mature systems are available to capture it, but solar energy systems are still considered to be an innovative technology. Adoption rates for solar and wind energy systems rise and fall with the political tides, and are relatively low in most rural areas in Arizona. This thesis tests the hypothesis that a consumer profile developed to characterize the adopters of renewable energy technology (RET) systems in rural Arizona is the same as the profile of other area residents who performed renovations, upgrades or additions to their homes. Residents of Santa Cruz and Cochise Counties who had obtained building permits to either install a solar or wind energy system or to perform a substantial renovation or upgrade to their home were surveyed to gather demographic, psychographic and behavioristic data. The data from 133 survey responses (76 from RET adopters and 57 from non-adopters) provided insights about their decisions regarding whether or not to adopt a RET system. The results, which are statistically significant at the 99% level of confidence, indicate that RET adopters had smaller households, were older and had higher education levels and greater income levels than the non-adopters. The research also provides answers to three related questions: First, are the energy conservation habits of RET adopters the same as those of non-adopters? Second, what were the sources of information consulted and the most important factors that motivated the decision to purchase a solar or wind energy system? And finally, are any of the factors which influenced the decision to live in a rural area in southeastern Arizona related to the decision to purchase a renewable energy system? The answers are provided, along with a series of recommendations that are designed to inform marketers and other promoters of RETs about how to utilize these results to help achieve their goals.
ContributorsPorter, Wayne Eliot (Author) / Reddy, T. Agami (Thesis advisor) / Pasqualetti, Martin (Committee member) / Larson, Kelli (Committee member) / Kennedy, Linda (Committee member) / Arizona State University (Publisher)
Created2011
Description
Telomerase ribonucleoprotein is a unique reverse transcriptase that adds telomeric DNA repeats to chromosome ends. Telomerase RNA (TER) is extremely divergent in size, sequence and has to date only been identified in vertebrate, yeast, ciliate and plant species. Herein, the identification and characterization of TERs from an evolutionarily distinct group, filamentous fungi, is presented. Based on phylogenetic analysis of 69 TER sequences and mutagenesis analysis of in vitro reconstituted Neurospora telomerase, we discovered a conserved functional core in filamentous fungal TERs sharing homologous structural features with vertebrate TERs. This core contains the template-pseudoknot and P6/P6.1 domains, essential for enzymatic activity, which retain function in trans. The in vitro reconstituted Neurospora telomerase is highly processive, synthesizing canonical TTAGGG repeats. Similar to Schizosaccharomycetes pombe, filamentous fungal TERs utilize the spliceosomal splicing machinery for 3' processing. Neurospora telomerase, while associating with the Est1 protein in vivo, does not bind homologous Ku or Sm proteins found in both budding and fission yeast telomerase holoenzyme, suggesting a unique biogenesis pathway. The development of Neurospora as a model organism to study telomeres and telomerase may shed light upon the evolution of the canonical TTAGGG telomeric repeat and telomerase processivity within fungal species.
ContributorsQi, Xiaodong (Author) / Chen, Julian (Thesis advisor) / Ghirlanda, Giovanna (Committee member) / Chaput, John (Committee member) / Arizona State University (Publisher)
Created2011
Description
ATP synthase is a large multimeric protein complex responsible for generating the energy molecule adenosine triphosphate (ATP) in most organisms. The catalysis involves the rotation of a ring of c-subunits, which is driven by the transmembrane electrochemical gradient. This dissertation reports how the eukaryotic c-subunit from spinach chloroplast ATP synthase has successfully been expressed in Escherichia coli and purified in mg quantities by incorporating a unique combination of methods. Expression was accomplished using a codon optimized gene for the c-subunit, and it was expressed as an attachment to the larger, more soluble, native maltose binding protein (MBP-c1). The fusion protein MBP-c1 was purified on an affinity column, and the c1 subunit was subsequently severed by protease cleavage in the presence of detergent. Final purification of the monomeric c1 subunit was accomplished using reversed phase column chromatography with ethanol as an eluent. Circular dichroism spectroscopy data showed clear evidence that the purified c-subunit is folded with the native alpha-helical secondary structure. Recent experiments appear to indicate that this monomeric recombinant c-subunit forms an oligomeric ring that is similar to its native tetradecameric form when reconstituted in liposomes. The F-type ATP synthase c-subunit stoichiometry is currently known to vary from 8 to 15 subunits among different organisms. This has a direct influence on the metabolic requirements of the corresponding organism because each c-subunit binds and transports one H+ across the membrane as the ring makes a complete rotation. The c-ring rotation drives rotation of the gamma-subunit, which in turn drives the synthesis of 3 ATP for every complete rotation. The availability of a recombinantly produced c-ring will lead to new experiments which can be designed to investigate the possible factors that determine the variable c-ring stoichiometry and structure.
ContributorsLawrence, Robert Michael (Author) / Fromme, Petra (Thesis advisor) / Chen, Julian J.L. (Committee member) / Woodbury, Neal W. (Committee member) / Arizona State University (Publisher)
Created2011
Description
Telomerase is a specialized enzyme that adds telomeric DNA repeats to the chromosome ends to counterbalance the progressive telomere shortening over cell divisions. It has two essential core components, a catalytic telomerase reverse transcriptase protein (TERT), and a telomerase RNA (TR). TERT synthesizes telomeric DNA by reverse transcribing a short template sequence in TR. Unlike TERT, TR is extremely divergent in size, sequence and structure and has only been identified in three evolutionarily distant groups. The lack of knowledge on TR from important model organisms has been a roadblock for vigorous studies on telomerase regulation. To address this issue, a novel in vitro system combining deep-sequencing and bioinformatics search was developed to discover TR from new phylogenetic groups. The system has been validated by the successful identification of TR from echinoderm purple sea urchin Strongylocentrotus purpuratus. The sea urchin TR (spTR) is the first invertebrate TR that has been identified and can serve as a model for understanding how the vertebrate TR evolved with vertebrate-specific traits. By using phylogenetic comparative analysis, the secondary structure of spTR was determined. The spTR secondary structure reveals unique sea urchin specific structure elements as well as homologous structural features shared by TR from other organisms. This study enhanced the understanding of telomerase mechanism and the evolution of telomerase RNP. The system that was used to identity telomerase RNA can be employed for the discovery of other TR as well as the discovery of novel RNA from other RNP complex.
ContributorsLi, Yang (Author) / Chen, Julian Jl (Thesis advisor) / Yan, Hao (Committee member) / Ghirlanda, Giovanna (Committee member) / Arizona State University (Publisher)
Created2011
Description
ABSTRACT The purpose of this study was to determine the energy cost of four modes of resistance training (push-ups, pull-ups, curl-ups, lunges). Twelve well trained men aged 23.6 (SD=2.84) years were recruited to participate in the study. Each of the 12 men completed three trials of each of the four exercises on one visit to the laboratory lasting slightly over one hour (M=72 min, SD=5.9 min). The oxygen consumption of the men was monitored constantly throughout the trial and data was recorded every five seconds. Mean VO2 values were calculated for each exercise. The values for push-ups (M=11.57 ml/kg/min, SD=1.99), curl-ups (M=10.99 ml/kg/min, SD=1.48), pull-ups (M=10.87 ml/kg/min, SD=2.51), and lunges (M=14.18 ml/kg/min, SD=1.78) were converted to METs (Metabolic Equivalents). The MET values (3.31, 3.14, 3.11, and 4.05 respectively) all fall within the range of moderate intensity activity. The findings of this study show that a single set of any of the above exercises will qualify as a moderate intensity activity and can be used to meet recommendations on daily physical activity.
ContributorsVezina, Jesse (Author) / Ainsworth, Barbara (Thesis advisor) / Campbell, Kathryn (Committee member) / Woodruff, Larry (Committee member) / Arizona State University (Publisher)
Created2011
Description
Redundant Binary (RBR) number representations have been extensively used in the past for high-throughput Digital Signal Processing (DSP) systems. Data-path components based on this number system have smaller critical path delay but larger area compared to conventional two's complement systems. This work explores the use of RBR number representation for implementing high-throughput DSP systems that are also energy-efficient. Data-path components such as adders and multipliers are evaluated with respect to critical path delay, energy and Energy-Delay Product (EDP). A new design for a RBR adder with very good EDP performance has been proposed. The corresponding RBR parallel adder has a much lower critical path delay and EDP compared to two's complement carry select and carry look-ahead adder implementations. Next, several RBR multiplier architectures are investigated and their performance compared to two's complement systems. These include two new multiplier architectures: a purely RBR multiplier where both the operands are in RBR form, and a hybrid multiplier where the multiplicand is in RBR form and the other operand is represented in conventional two's complement form. Both the RBR and hybrid designs are demonstrated to have better EDP performance compared to conventional two's complement multipliers. The hybrid multiplier is also shown to have a superior EDP performance compared to the RBR multiplier, with much lower implementation area. Analysis on the effect of bit-precision is also performed, and it is shown that the performance gain of RBR systems improves for higher bit precision. Next, in order to demonstrate the efficacy of the RBR representation at the system-level, the performance of RBR and hybrid implementations of some common DSP kernels such as Discrete Cosine Transform, edge detection using Sobel operator, complex multiplication, Lifting-based Discrete Wavelet Transform (9, 7) filter, and FIR filter, is compared with two's complement systems. It is shown that for relatively large computation modules, the RBR to two's complement conversion overhead gets amortized. In case of systems with high complexity, for iso-throughput, both the hybrid and RBR implementations are demonstrated to be superior with lower average energy consumption. For low complexity systems, the conversion overhead is significant, and overpowers the EDP performance gain obtained from the RBR computation operation.
ContributorsMahadevan, Rupa (Author) / Chakrabarti, Chaitali (Thesis advisor) / Kiaei, Sayfe (Committee member) / Cao, Yu (Committee member) / Arizona State University (Publisher)
Created2011
Description
Natural products that target the DNA of cancer cells have been an important source of knowledge and understanding in the development of anticancer chemotherapeutic agents. Bleomycin (BLM) exemplifies this class of DNA damaging agent. The ability of BLM to chelate metal ions and effect oxidative damage of the deoxyribose sugar moiety of DNA has been studied extensively for four decades. Here, the study of BLM A5 was conducted using a previously isolated library of hairpin DNAs found to bind strongly to metal free BLM. The ability of BLM to effect single-stranded was then extensively characterized on both the 3′ and 5′-arms of the hairpin DNAs. The strongly bound DNAs were found to be efficient substrates for Fe·BLM A5-mediated cleavage. Surprisingly, the most prevalent site of damage by BLM was found to be a 5′-AT-3′ dinucleotide sequence. This dinucleotide sequence and others generally not cleaved by BLM when examined using arbitrarily chosen DNA substrate were found in examining the library of ten hairpin DNAs. In total, 111 sites of DNA damage were found to be produced by exposure of the hairpin DNA library to Fe·BLM A5. Also, an assay was developed with which to test the propensity of the hairpin DNAs to undergo double stranded DNA damage. Adapting methods previously described by the Povirk laboratory, one hairpin was characterized using this method. The results were in accordance with those previously reported.
ContributorsSegerman, Zachary (Author) / Hecht, Sidney M. (Thesis advisor) / Levitus, Marcia (Committee member) / Ghirlanda, Giovanna (Committee member) / Arizona State University (Publisher)
Created2011