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- All Subjects: Agriculture
- Creators: School of International Letters and Cultures
Protein and gene circuit level synthetic bioengineering can require years to develop a single target. Phage assisted continuous evolution (PACE) is a powerful new tool for rapidly engineering new genes and proteins, but the method requires an automated cell culture system, making it inaccessible to non industrial research programs. Complex protein functions, like specific binding, require similarly dynamic PACE selection that can be alternatively induced or suppressed, with heat labile chemicals like tetracycline. Selection conditions must be controlled continuously over days, with adjustments made every few minutes. To make PACE experiments accessible to the broader community, we designed dedicated cell culture hardware and integrated optogenetically controlled plasmids. The low cost and open source platform allows a user to conduct PACE with continuous monitoring and precise control of evolution using light.
Animal agriculture is a growing industry worldwide as the global demand for animal products increases. This has resulted in many harmful unintended consequences for human health, the environment, and animal welfare. This paper aims to uncover the hidden costs of negative externalities by answering the question: What types of subsidies is the US government distributing to the animal agriculture industry and in what amount? This paper will begin with some background on a few of the externalities created by the animal agriculture industry focusing specifically on environmental issues of water, air, and deforestation. Once this background is established, this will show that animal agriculture is in fact a negative-externality-generating industry. Next, subsidies will be defined and the principal findings of the research will reveal the different forms of support that the US government provides to animal agriculture. Lastly, these subsidies, both direct and indirect, will be quantified.