Protein and gene circuit level synthetic bioengineering can require years to develop a single target. Phage assisted continuous evolution (PACE) is a powerful new tool for rapidly engineering new genes and proteins, but the method requires an automated cell culture system, making it inaccessible to non industrial research programs. Complex protein functions, like specific binding, require similarly dynamic PACE selection that can be alternatively induced or suppressed, with heat labile chemicals like tetracycline. Selection conditions must be controlled continuously over days, with adjustments made every few minutes. To make PACE experiments accessible to the broader community, we designed dedicated cell culture hardware and integrated optogenetically controlled plasmids. The low cost and open source platform allows a user to conduct PACE with continuous monitoring and precise control of evolution using light.

AIR, being short for the Arts Initiative for Refugees, is a new, self-founded organization that provides refugee youth with sessions, programs, and opportunities to help them achieve artistic and personal success through teaching and mentorship. AIR strives to offer individualized and group mentoring programs to its students designed for their unique interests in the arts fields of their choice. The immediate objective of this organization, which my thesis was founded upon, was to introduce refugee youth at a newcomer school, Valencia Newcomer School, to various new art forms utilizing direct application, to play and have fun, to create a sense of community and support, to create a safe, inclusive environment, to help the students learn more about each other's cultures and backgrounds, and to allow the students to create art work that is genuine to them and their backgrounds. The ultimate goal of AIR is to branch out to become a small-scale, national/international non-profit organization.