This dissertation explores the technolinguistic brokering experience of adolescents and (im)migrant non-English speaking mothers in acculturating families. By focusing on the performance of cultural intermediation, I examine the dimensions of technolinguistic brokering and their influence upon the Adolescent Language Technology Broker (ALTB) and mother relationship. Additionally, I explore the factors of power present as a result of the complexities of the ALTBs role to connect their mother to the English speaking community. This research uses a qualitative approach to explore concepts of expertise, knowledge, (inter)dependence, relational maintenance and quality, and power in the dialogic cultural relationship. Research indicates that expertise in the form of culture, cultural interactions, multilingual, and relational maintenance and quality contribute to the ALTBs capabilities in building cultural relationships. Moreover, to assist in dealing with power tensions created by differing levels of expertise and knowledge, ALTBs and mothers communicatively construct an (inter)dependent cultural relationship. I highlight practical implications, discuss limitations, and provide recommendations for future directions.

Agassiz’s desert tortoise (Gopherus agassizii) is a long-lived species native to the Mojave Desert and is listed as threatened under the US Endangered Species Act. To aid conservation efforts for preserving the genetic diversity of this species, we generated a whole genome reference sequence with an annotation based on deep transcriptome sequences of adult skeletal muscle, lung, brain, and blood. The draft genome assembly for G. agassizii has a scaffold N50 length of 252 kbp and a total length of 2.4 Gbp. Genome annotation reveals 20,172 protein-coding genes in the G. agassizii assembly, and that gene structure is more similar to chicken than other turtles. We provide a series of comparative analyses demonstrating (1) that turtles are among the slowest-evolving genome-enabled reptiles, (2) amino acid changes in genes controlling desert tortoise traits such as shell development, longevity and osmoregulation, and (3) fixed variants across the Gopherus species complex in genes related to desert adaptations, including circadian rhythm and innate immune response. This G. agassizii genome reference and annotation is the first such resource for any tortoise, and will serve as a foundation for future analysis of the genetic basis of adaptations to the desert environment, allow for investigation into genomic factors affecting tortoise health, disease and longevity, and serve as a valuable resource for additional studies in this species complex.

Data Availability: All genomic and transcriptomic sequence files are available from the NIH-NCBI BioProject database (accession numbers PRJNA352725, PRJNA352726, and PRJNA281763). All genome assembly, transcriptome assembly, predicted protein, transcript, genome annotation, repeatmasker, phylogenetic trees, .vcf and GO enrichment files are available on Harvard Dataverse (doi:10.7910/DVN/EH2S9K).