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- All Subjects: Nutrition
- Creators: Alexon, Christy
Description
In my creative project, I decided to shadow a naturopathic doctor to experience what the process of naturopathic treatment entails. I conducted interviews with patients and Dr. Raisanen to explore their backgrounds and motivations, and read studies he recommended that explained some of the science behind his methods of treatment, such as fasting, dietary adjustment (a plant based diet), sodium and saturated/trans fats reduction, caffeine reduction, exercise, stress modulation, supplements, and sleep adjustments. I wrote small expositional summaries on these studies. I also took measurements recorded by Dr. Raisanen through the course of treatments with different patients to produce visuals of changes in body composition. I finally produced a small handout with Dr. Raisanen's help that summarized on a page the basics of lifestyle changes that a naturopathic doctor would want a patient to undertake.
ContributorsSkoog, David Alexander (Author) / Alexon, Christy (Thesis director) / Raisanen, Peter (Committee member) / Department of Finance (Contributor) / Department of Management and Entrepreneurship (Contributor) / Department of Supply Chain Management (Contributor) / Barrett, The Honors College (Contributor)
Created2018-12
Description
The medicinal use of vinegar dates to ancient times, and empirical evidence has mounted over the past several decades supporting health benefits from vinegar ingestion, including reductions in blood glucose, blood pressure, and blood cholesterol. Additionally, although clinical trial data are mixed, there are several reports demonstrating reductions in body mass and fat mass with daily vinegar ingestion. This blinded, randomized placebo-controlled trial examined the impact of daily vinegar ingestion on anthropometric measures in healthy young adults recruited from a campus population. Participants (n=28; aged 25.8±7.0 y; body mass index [BMI] > 23 kg/m2) were stratified by age, sex, and BMI and randomly assigned to the liquid [VIN] or pill [CON] groups. VIN participants diluted 2 tablespoons of red wine vinegar (750 mg acetic acid; Pompeian Inc.) in 8-12 ounces of water to drink with food at mealtime twice daily. CON participants consumed 1 vinegar pill daily (22.5 mg acetic acid; Spring Valley). All participants provided written consent for this IRB approved trial. The study lasted four weeks, and anthropometric measurements were conducted in a fasted state at weeks 0 and 4. Study adherence varied slightly (90±17% and 100±14% for VIN and CON respectively, p=0.029); hence, adherence was controlled for in all analyses. Changes in BMI (-0.1±0.5 and +0.1±0.3 kg/m2, p=0.127) and body weight (-0.3±1.4 and +0.1±1.1 kg, p=0.158) did not differ significantly between VIN and CON groups, respectively. However, both waist circumference and percent body fat were reduced significantly for VIN participants in comparison to CON participants (-0.5±1.3 vs. +0.6±2.4 cm [p=0.026]and -0.4±0.7 vs. +0.3±1.0% [p=0.045]. Although the observed differences between groups are modest, the study was short in duration and this protocol did not apply energy restriction or exercise interventions, suggesting a possible benefit of vinegar ingestion on adiposity.
ContributorsMeyer, Lexie Rae (Author) / Johnston, Carol (Thesis advisor) / Alexon, Christy (Committee member) / Grant, Shauna (Committee member) / Arizona State University (Publisher)
Created2024
Description
Diabetes is the 7th leading cause of death globally. In 2018, 34.2 million Americans had type 2 diabetes. Many symptoms of diabetes are similar to those of scurvy or vitamin C deficiency. Vitamin C marginality and inadequacy are more prevalent in Type 2 Diabetes/prediabetes than with normal glucose tolerance. Intracellular vitamin C inadequacy is suspected due to competition between dehydroascorbic acid and glucose at GLUT 1 and 3 cellular receptors. Erythrocyte osmotic fragility is noted in Gulo -/- knockout mice unable to synthesize endogenous vitamin C. The ascorbate deficient red blood cells presented with low cytoskeletal B-spectrin, spherocyte appearance, and impaired deformability. This cross-sectional study investigated the relationships between diabetes status, erythrocyte osmotic fragility, and serum vitamin C status. Participants were aged 18-65, non-smoking, reported no unresolved health complications, and denied prior vitamin C supplementation. Those with T2D indicated diagnosis of >1 year. All participants provided written informed consent and the study was approved by the local Institutional Review Board in January 2021. Participants provided one fasted blood sample. Erythrocyte osmotic fragility was measured via UV/Vis spectrophotometry with various concentrations of sodium chloride (0.85% - 0.10%) to induce osmotic stress. In addition, plasma was extracted and mixed 1:1 with 10% (w/v) metaphosphoric acid in 2 mmol/L disodium EDTA and centrifuged. The supernatant was stored at -80°C until analysis with isocratic reverse-phase UV-HPLC separation.
Participant characteristics did not differ significantly between groups apart from age (p< 0.01) and HbA1c (p=0.002). Data are presented for adults with T2D (n=14; 36% female; 55.5±8.2 y; 31.5±9.0 kg/m2; HbA1c: 7.4±1.9%; plasma vitamin C: 36.0±12.2 uM) and without T2D (n=16; 69% female; 38.7±13.5 y; 26.8±6.6 kg/m2; A1c: 5.4±0.3%; plasma vitamin C: 34.8±10.9uM). Erythrocyte osmotic fragility was significantly elevated (+4.4% hemolysis) in adults without T2D at 0.35% saline (p=0.039). Greater VC status (>30 uM) was associated with lower hemolysis at 0.35% NaCl (p=0.031). Erythrocyte osmotic stability was linked to greater vitamin C intake at 0.20% saline in those without T2D (p =0.019).
In this pilot study, vitamin C status did not differ significantly by diabetes status. Vitamin C status was directly linked to erythrocyte osmotic stability in adults without T2D.
ContributorsLundy, Ciara Cheyanne (Author) / Johnston, Carol (Thesis advisor) / Sweazea, Karen (Committee member) / Alexon, Christy (Committee member) / Arizona State University (Publisher)
Created2022
Description
Objective: This research examined the impact of daily ingestions of commercial high protein nutrition bars (with or without added fiber) on 24-h energy intake and satiety
for one week among free-living young healthy adults.
Design: In a 4-week double-blind, randomized crossover trial, 21 normal and
overweight participants (Mean BMI 23.9 ± 2.7 kg/m²), free of chronic diseases, were
randomized assigned to HP (high protein: 21 g protein) or HPHF (high protein high fiber:
20g, 14 g fiber) nutrition bars. Participants were included in the trial if they meet the
criteria for non-smoking, and not taking prescribed medication for chronic diseases.
Participants were instructed to consume commercial nutrition bars daily for seven
consecutive days. Body composition was measured with a bioelectrical impedance scale
at weeks 1, 3, and 5. Dietary data was recorded by the MyFitnessPal app on Wednesday,
Friday, and Sunday of each week.
Results: The mean energy intake for the weeks HPHF bars were consumed is
significantly higher compared to baseline (1998 ± 534 vs. 1806 ± 537 respectively; p =
0.035). The mean fat mass following one week of HPHF bar consumption was
significantly higher than the baseline value (18.8 ± 6.8 vs. 18.3 ± 6.7 respectively; p =
0.023) and trended higher (18.8 ± 6.8 vs. 18.3 ± 6.7 respectively; p = 0.057) in
comparison to the value following one week of HP bar consumption. For the high
physical activity level group (n = 10), the mean energy intakes for the baseline week and
the weeks the HP and HPHF bars were consumed were 1883 ± 597 kcal, 2154 ± 712 kcal,
and 2099 ± 603 kcal respectively (p ˂ 0.04; energy intakes for both bars were
significantly different from baseline). Nutrient intakes differed significantly mirroring the
nutrient profile for each specific bar. There are significant effects after both bars on
satiety, but there were no differences between each bar.
Conclusions: Sales of nutrition bars gained rapid growth and may represent a
unique source for specific nutrients. However, ingestion of commercial high protein
nutrition bars may increase the risk of gaining fat mass and eventual body mass over
time.
ContributorsPang, Minghan (Author) / Johnston, Carol (Thesis advisor) / Shepard, Christina (Committee member) / Alexon, Christy (Committee member) / Arizona State University (Publisher)
Created2022
Description
This thesis aims to properly prepare athletes for the significant life changes that will impact them as they transition from college or amateur athletics to professional sports careers. This thesis also identifies the benefits that reap from early education on expectations of learning what to expect at an earlier point in their amateur career. Certain areas are struck as having increasing importance in the transition: financial preparation, mental health and mental strengthening, public relations practice, nutritional needs, and other lifestyle choices that can help athletes reach their potential. Improving education in these areas, preparing athletes for these changes, and showing examples of what to expect as they transition into professional sports can benefit the athletes, the universities and colleges in which they are educated, the franchises in which they are drafted, and the communities where they reside. This information can be delivered through a handbook while having in-person training that can build upon each session to dive deeper into each given topic while building relationships with the athletes.
ContributorsSpies, Lindsey (Author) / Murphy, Flynn (Co-author) / Eaton, John (Thesis director) / McIntosh, Daniel (Committee member) / Barrett, The Honors College (Contributor) / Department of Marketing (Contributor)
Created2023-05
Description
This thesis aims to properly prepare athletes for the significant life changes that will impact them as they transition from college or amateur athletics to professional sports careers. This thesis also identifies the benefits that reap from early education on expectations of learning what to expect at an earlier point in their amateur career. Certain areas are struck as having increasing importance in the transition: financial preparation, mental health and mental strengthening, public relations practice, nutritional needs, and other lifestyle choices that can help athletes reach their potential. Improving education in these areas, preparing athletes for these changes, and showing examples of what to expect as they transition into professional sports can benefit the athletes, the universities and colleges in which they are educated, the franchises in which they are drafted, and the communities where they reside. This information can be delivered through a handbook while having in-person training that can build upon each session to dive deeper into each given topic while building relationships with the athletes.
ContributorsMurphy, Flynn (Author) / Spies, Lindsey (Co-author) / McIntosh, Daniel (Thesis director) / Eaton, John (Committee member) / Barrett, The Honors College (Contributor) / Department of Marketing (Contributor)
Created2023-05
Description
The purpose of this study was to evaluate the impact of improved hydration on endurance performance and mood in physically active adults. Participants (n = 72; age, 21.0 ± 3.0; 22.2% female) completed two two-mile run trials separated by exactly a week. Before each trial, participants provided a urine sample from the day before the run and a sample from the morning of the run. These samples were analyzed for urine osmolality (UOsm), urine specific gravity (USG), and urine color (Ucol). UOsm and USG levels determined if the participants were placed in either the euhydrated or underhydrated group after the first trial. Those assigned to the euhydrated group were instructed to maintain their current fluid intake levels and those in the underhydrated group were instructed to increase fluid intake levels before the second trial. However, results were grouped by if they improved or maintained their hydration or not. The subjects also completed a Profile of Mood States (POMS) questionnaire before and after each trial to determine mood. Based on conditioning requirements for group assignment, 38% of subjects were classified as underhydrated. There were significant differences between the two trials for both subjects that improved and worsened their hydration in UOsm, USG, Ucol, and thirst (P < 0.05). The group with improved hydration ran -15 ± 67 sec faster in the second trial, while the group that worsened hydration ran 4 ± 26 sec slower in the second trial. When these differences were compared between the two groups with a t-test, there was a trend for statistical differences with a one-way t-test analysis (P = 0.06). When results were split by sex no statistically significant differences were observed (male: -10.8 ± 63.6 sec; female: -29.4 ± 94.8 sec; P > 0.05). Improved hydration did not result in statistically significant difference in TMD or any of the individual mood sub-scales for either group for both males and females (P > 0.05). In conclusion, increased fluid intake to optimize hydration status may affect endurance exercise in young, healthy adults in a two-mile run, but no effect was seen on mood.
ContributorsDoyle, Amanda Ann (Author) / Kavouras, Stavros (Thesis advisor) / Alexon, Christy (Committee member) / Wardenaar, Floris (Committee member) / Arizona State University (Publisher)
Created2022
Description
Vitamin D is a nutrient that is obtained through the diet and vitamin D supplementation and created from exposure to Ultraviolet B (UVB) radiation. While there are many factors that determine how much serum 25-hydroxyvitamin D (25(OH)D) concentration is in the body, little is known about how genetic variation in vitamin D-related genes influences serum 25(OH)D concentrations resulting from daily vitamin D intake and exposure to direct sunlight. Previous studies show that common genetic variants rs10741657 (CYP2R1), rs4588 (GC), rs228678 (GC), and rs4516035 (VDR) act as moderators and alter the effect of outdoor time and vitamin D intake on serum 25(OH)D concentrations. The objective of this study is to analyze the associations between serum 25(OH)D concentrations resulting from outdoor time and vitamin D intake, and genetic risk scores (GRS) established from previous studies involving single nucleotide polymorphisms (SNP) located on or near genes involving vitamin D synthesis, transport, activation, and degradation in 102 Hispanic and Non-Hispanic adults in the San Diego County, California. This study is a secondary analysis of data from the Community of Mine study. Global Positioning System (GPS) data collected by the Qstarz GPS device worn by each participant was used to measure outdoor time, a proxy measurement for sun exposure time. Vitamin D intake was assessed using two 24-hour dietary recalls. Blood samples were measured for serum 25(OH)D concentrations. DNA was provided to assess each participant for the various genetic variants. Adjusted analyses of the GRS and serum 25(OH)D concentrations showed that individuals with high GRS (3-4) had lower serum 25(OH)D concentrations than individuals with low GRS (0-2) for both Nissen GRS and Rivera-Paredez GRS.
ContributorsAnderson, Heather Ray (Author) / Sears, Dorothy (Thesis advisor) / Alexon, Christy (Committee member) / Dinu, Valentin (Committee member) / Jankowska, Marta (Committee member) / Arizona State University (Publisher)
Created2022
Description
Depression has been linked to a significant burden of disease, loss of life, and decreased efficacy of treatment for its comorbidities. Empirical evidence has mounted over the past several decades supporting health benefits from vinegar ingestion, including improvements in blood glucose management, blood cholesterol levels, and inflammation indicators. To date, there have not been any studies in human populations that explore the potential relationship between daily vinegar ingestion and changes in depression indicators and blood metabolomics. This blinded, randomized controlled trial examined the impact of twice-daily vinegar ingestion on mental health measures in healthy young adults recruited from a metropolitan setting. Participants (n=28; aged 25.8±7.0 y; body mass index [BMI] >23 kg/m2) were stratified by age, gender, and BMI and randomly assigned to the liquid (VIN) or pill (CON) groups. VIN participants ingested 2 tablespoons of red wine vinegar (~1550 mg acetic acid; Pompeian Inc., Appendix J) diluted in 8-12 ounces of water, consumed with food at mealtimes twice daily (total ~3100mg acetic acid daily). CON participants consumed 1 vinegar pill daily (22.5 mg acetic acid; Spring Valley, Appendix J). The study lasted four weeks, and anthropometric measurements were conducted in a fasted state at weeks 0 and 4. Study adherence varied slightly (90±17% and 100±14% for VIN and CON respectively, p=0.084); hence, adherence was controlled for in all subsequent analyses. Changes in L-tryptophan (p=0.777, η2=0.003), peripheral serotonin levels (p=0.348, η2=0.035), GABA (p=0.403, η2=0.028), and gut-mediated short-chain fatty acids acetic acid (p=0.355, η2=0.034), and propionic acid (p=0.383, η2=0.031), did not differ significantly between VIN and CON groups respectively with the exception of isobutyric acid (p=0.0374, q=0.0473). However, Patient Health Questionnaire (PHQ-9) depression scores improved significantly for VIN participants in comparison to CON participants (-0.5±1.3 vs. +0.7±2.4 cm [p=0.026] and -0.4±0.7 vs. +0.3±1.0% [p=0.045]. Although these differences between groups are modest, which would be expected given the short study duration, changes were not driven by pharmacological or lifestyle interventions, suggesting the benefits of vinegar ingestion on mental health symptomology and the blood metabolome.
ContributorsBarrong, Haley (Author) / Johnston, Carol (Thesis advisor) / Alexon, Christy (Committee member) / Roberts, Nicole (Committee member) / Arizona State University (Publisher)
Created2024
Description
Cardiovascular disease (CVD) is characterized by impaired vasodilation and the development of atherosclerosis.78 A diet high in saturated fat, such as palmitate, contributes to this by promoting inflammation and oxidative stress in human vascular smooth muscle cells (VSMC). 11,12,84,88 The inflammation cascade that occurs increases pro-inflammatory cytokines, like tumor necrosis factor alpha (TNF-alpha) and increases proinflammatory enzymes like cyclooxygenase 2 (COX-2) contributing to inflammation, oxidative stress, blood pressure shifts, and atherosclerosis.11,12,69,84 Palmitate has been found to upregulate TNF-alpha,85 and COX-2. 11,12, 84
In various studies, sumac, a Mediterranean spice and known antioxidant,39,7,66,67 has been shown to have antioxidant properties through its ability to inhibit reactive oxygen species (ROS) such as superoxide.39,7,66,67 Sumac has also been found to reduce TNF-alpha.100 Results from a study of hypertensive human subjects fed a sumac supplement showed a decrease in blood pressure.59
In the current study, COX-2 levels were determined to evaluate the level of inflammation in response to palmitate when primary aortic human vascular smooth muscle cells (HAoVSM) were treated with sumac. The treatments included: vehicle (bovine serum albumin), 100 µM palmitate, and 10, 20, 40, 60, and 80 µg/mL sumac. Sumac did not alter COX-2 protein levels between vehicle and sumac groups. Additional studies were designed to examine whether 80 µg/mL sumac could reverse impaired vasodilation caused by 10 weeks of high fat intake, consisting of 60% of total calories from fat, in Sprague-Dawley rats. Mesenteric arteries were isolated and exposed to sumac. High fat diet (HFD) arteries had impaired vasodilation compared to arteries from chow-fed fats. HFD arteries exposed to sumac had similar endothelium-dependent vasodilation responses as those not exposed to sumac, however, there were trends for improved vasodilation. I suggest that sumac likely exhibits antioxidant capabilities that prevent superoxide from decreasing the bioavailability of nitric oxide in the vasculature, thus promoting endothelium-dependent vasodilation and preventing the creation of more harmful reactive oxygen species. Isolated arteries from chow fed rats developed irreversible vasodilation when exposed to sumac and were therefore not responsive to pre-constriction with phenylephrine (PE) likely related to nitrates and gallic acid naturally present in sumac whereby inhibiting PE.
In various studies, sumac, a Mediterranean spice and known antioxidant,39,7,66,67 has been shown to have antioxidant properties through its ability to inhibit reactive oxygen species (ROS) such as superoxide.39,7,66,67 Sumac has also been found to reduce TNF-alpha.100 Results from a study of hypertensive human subjects fed a sumac supplement showed a decrease in blood pressure.59
In the current study, COX-2 levels were determined to evaluate the level of inflammation in response to palmitate when primary aortic human vascular smooth muscle cells (HAoVSM) were treated with sumac. The treatments included: vehicle (bovine serum albumin), 100 µM palmitate, and 10, 20, 40, 60, and 80 µg/mL sumac. Sumac did not alter COX-2 protein levels between vehicle and sumac groups. Additional studies were designed to examine whether 80 µg/mL sumac could reverse impaired vasodilation caused by 10 weeks of high fat intake, consisting of 60% of total calories from fat, in Sprague-Dawley rats. Mesenteric arteries were isolated and exposed to sumac. High fat diet (HFD) arteries had impaired vasodilation compared to arteries from chow-fed fats. HFD arteries exposed to sumac had similar endothelium-dependent vasodilation responses as those not exposed to sumac, however, there were trends for improved vasodilation. I suggest that sumac likely exhibits antioxidant capabilities that prevent superoxide from decreasing the bioavailability of nitric oxide in the vasculature, thus promoting endothelium-dependent vasodilation and preventing the creation of more harmful reactive oxygen species. Isolated arteries from chow fed rats developed irreversible vasodilation when exposed to sumac and were therefore not responsive to pre-constriction with phenylephrine (PE) likely related to nitrates and gallic acid naturally present in sumac whereby inhibiting PE.
ContributorsBarberes, Julia (Author) / Sweazea, Karen (Thesis advisor) / Gonzales, Rayna (Committee member) / Alexon, Christy (Committee member) / Arizona State University (Publisher)
Created2018