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- Creators: School of Life Sciences
- Creators: DeNardo, Dale
In order to address this, multiple comparative genomics and bioinformatics analyses were conducted to elucidate patterns of evolution in the green anole and across multiple anole species. Comparative genomics analyses were used to infer additional X-linked loci in the green anole, RNAseq data from male and female samples were anayzed to quantify patterns of sex-biased gene expression across the genome, and the extent of dosage compensation on the anole X chromosome was characterized, providing evidence that the sex chromosomes in the green anole are dosage compensated.
In addition, X-linked genes have a lower ratio of nonsynonymous to synonymous substitution rates than the autosomes when compared to other Anolis species, and pairwise rates of evolution in genes across the anole genome were analyzed. To conduct this analysis a new pipeline was created for filtering alignments and performing batch calculations for whole genome coding sequences. This pipeline has been made publicly available.
Animal shelters are stressful environments for dogs and a plethora of research has been conducted on interventions aimed at improving the welfare of these animals. One type of intervention is social interaction, either between dogs and people or dogs and conspecifics. To investigate the types of social interaction dogs engage in and the impact of that contact on their welfare, 12 dogs were enrolled to participate in group sessions with other dogs, supervised by staff, in a shelter setting. There were three, 15-minute sessions per day across three days in which groups of two to four dogs were observed and recorded on video. These videos were then analyzed per dog for three types of interactions: dog-dog, dog-human, and dog-environment. It was found that the dogs spent significantly more time engaging with the staff members in the room than with conspecifics or the environment. Physiological measurements, including cortisol and S-IgA levels, were taken using urinary and fecal samples obtained both in the morning prior to these interaction sessions and after the final interaction of the day. No significant correlations were found between the amount of time that the dogs spent in each type of interaction and dogs’ cortisol or S-IgA levels. However, smaller statistical effects suggest that human interaction may correspond with decreased stress the day after interaction while conspecific interaction may be related to increases in stress the following day. Overall, these findings suggest that social interaction, particularly with people, may be beneficial, and should be further explored as a method to enhance the well-being of shelter dogs.