Matching Items (38)
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- All Subjects: Sustainability
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Description
The purpose of this project was to examine the viability of protein biomarkers in pre-symptomatic detection of lung cancer. Regular screening has been shown to vastly improve patient survival outcome. Lung cancer currently has the highest occurrence and mortality of all cancers and so a means of screening would be highly beneficial. In this research, the biomarker neuron-specific enolase (Enolase-2, eno2), a marker of small-cell lung cancer, was detected at varying concentrations using electrochemical impedance spectroscopy in order to develop a mathematical model of predicting protein expression based on a measured impedance value at a determined optimum frequency. The extent of protein expression would indicate the possibility of the patient having small-cell lung cancer. The optimum frequency was found to be 459 Hz, and the mathematical model to determine eno2 concentration based on impedance was found to be y = 40.246x + 719.5 with an R2 value of 0.82237. These results suggest that this approach could provide an option for the development of small-cell lung cancer screening utilizing electrochemical technology.
ContributorsEvans, William Ian (Author) / LaBelle, Jeffrey (Thesis director) / Spano, Mark (Committee member) / Barrett, The Honors College (Contributor) / Harrington Bioengineering Program (Contributor)
Created2014-05
Description
Although sustainability as a concept and a science has been around for quite some time, it has only recently come into the common vernacular of citizens around the world. While there are a number of arguments that have been and can be made about the role of sustainability in developing countries, it can be said with certainty that sustainability education, especially at the pre-university level, is commonly neglected even in countries that have sustainability initiatives elsewhere in their systems. Education is an important part of development in any country, and sustainability education is critical to raising generations who are more aware of the connections in the world around them. Informal education, or education that takes place outside of a formal classroom, can provide an especially important platform for sustainability ideas. These factors take on unique characteristics within the environment of a small island with noble sustainability goals but limited resources and an economy that includes a significant domestic goat population. After providing basic background on sustainability and the nature of the educational process within the environment of the small island-nation of Grenada, I discuss the importance of informal education and follow my path with a local non-profit in Grenada leading to the development of a locally-relevant sustainability curriculum for implementation in a K-6 school.
ContributorsMelkonoff, Natalie Anne (Author) / Eder, James (Thesis director) / BurnSilver, Shauna (Committee member) / Barrett, The Honors College (Contributor) / School of International Letters and Cultures (Contributor) / School of Sustainability (Contributor) / School of Life Sciences (Contributor)
Created2015-05
Description
Locusts are a major crop pest in many parts of the world and different species are endemic to different countries. In Latin America, the South American Locust (Schistocerca cancellata) is the predominant species found mostly in Argentina, Chile, Bolivia, Paraguay, and southern Brazil with Argentina being the most affected. Several control and management practices, including biological control, have been implemented in these countries in the past to control the locusts and reduce their impact on crop and vegetation, however, effective long-term control and management practices will require a detail understanding of how the predominant locust species in this region responds to resource variation. Research has shown that there is strong evidence that locusts, and many other organisms, will actively balance dietary macronutrients (protein, carbohydrates, and lipids) to optimize growth, survival, and/or reproduction. A study by Cease et. al, 2017, on the dietary preferences of the Mongolian locust (Oedaleus asiaticus) showed that it prefers diets that are high in carbohydrates over diets that are high in protein, in this case locusts self-selected a 1:2 ratio of protein:carbohydrate. This and many other studies provide vital insight into the nutritional and feeding preferences of these locust species but the effects that this difference in protein: carbohydrate preferences has on growth, egg production, flight potential, and survival has yet to be fully explored, hence, this study investigates the effects that nitrogen fertilization of wheatgrass will have on the growth, egg production, survival, and flight muscle mass of the South American locust in a controlled, laboratory environment.
ContributorsManneh, Balanding (Author) / Cease, Arianne (Thesis director) / Overson, Rick (Committee member) / School of Sustainability (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
In this paper, β-estradiol was characterized utilizing electrochemical impedance spectroscopy (EIS) techniques for the purpose of developing a multi-marker fertility sensor. β-estradiol was immobilized onto the surface of gold disk electrodes to find the optimal binding frequency of estradiol and its respective antibody, anti-17β-estradiol, which was determined to be 37.46Hz. At this frequency a logarithmic relationship between concentration and impedance (Z/ohm) was established creating a concentration calibration curve with a slope of 211 ohm/ln(pg mL-1), an R-squared value of 0.986 and a lower limit of detection of 742 fg mL-1. The specificity and cross-reactivity of the antibody with other hormones was tested through interferent and non-target experiments. Signal-to-noise ratio analysis verified that anti-17β-estradiol exhibited minimal chemical reactions with other hormones (SNR< 3) in non-target experiments. Additionally, there were minimal changes in the amount of signal collected during interferent testing, with albumin and follicle stimulating hormone having SNR values greater than 3. These results, along with the unique frequency response of the antibody-target binding reaction, allow for the possibility of using anti-17β-estradiol and β-estradiol for detecting multiple fertility biomarkers on a single sensor.
ContributorsSmith, Victoria Ann (Author) / LaBelle, Jeffrey (Thesis director) / Spano, Mark (Committee member) / Barrett, The Honors College (Contributor) / Harrington Bioengineering Program (Contributor)
Created2014-05
Description
Diabetes mellitus is a disease characterized by many chronic and acute conditions. With the prevalence and cost quickly increasing, we seek to improve on the current standard of care and create a rapid, label free sensor for glycated albumin (GA) index using electrochemical impedance spectroscopy (EIS). The antibody, anti-HA, was fixed to gold electrodes and a sine wave of sweeping frequencies was induced with a range of HA, GA, and GA with HA concentrations. Each frequency in the impedance sweep was analyzed for highest response and R-squared value. The frequency with both factors optimized is specific for both the antibody-antigen binding interactions with HA and GA and was determined to be 1476 Hz and 1.18 Hz respectively in purified solutions. The correlation slope between the impedance response and concentration for albumin (0 \u2014 5400 mg/dL of albumin) was determined to be 72.28 ohm/ln(mg/dL) with an R-square value of 0.89 with a 2.27 lower limit of detection. The correlation slope between the impedance response and concentration for glycated albumin (0 \u2014 108 mg/dL) was determined to be -876.96 ohm/ln(mg/dL) with an R-squared value of 0.70 with a 0.92 mg/dL lower limit of detection (LLD). The above data confirms that EIS offers a new method of GA detection by providing unique correlation with albumin as well as glycated albumin. The unique frequency response of GA and HA allows for modulation of alternating current signals so that several other markers important in the management of diabetes could be measured with a single sensor. Future work will be necessary to establish multimarker sensing on one electrode.
ContributorsEusebio, Francis Ang (Author) / LaBelle, Jeffrey (Thesis director) / Pizziconi, Vincent (Committee member) / Barrett, The Honors College (Contributor) / Harrington Bioengineering Program (Contributor)
Created2014-05
DescriptionThis paper provides an analysis of the differences in impacts made by companies that promote their sustainability efforts. A comparison of companies reveals that the ones with greater supply chain influence and larger consumer bases can make more concrete progress in terms of accomplishment for the sustainability realm.
ContributorsBeaubien, Courtney Lynn (Author) / Anderies, John (Thesis director) / Allenby, Brad (Committee member) / Janssen, Marco (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor)
Created2013-05
Description
Acetaminophen, commonly found in Tylenol and other over the counter (OTC) pharmaceuticals, was electrochemically characterized on custom made, flexible, screen printed electrodes (SPEs) to serve as a model target pharmaceutical found in flowing water lines. Carbon, silver/silver chloride, and insulator paste inks were printed onto polyethylene naphthalateolyester (PEN) using custom made stencils for a 4x1 array of 3-electrode electrochemical cells. Cyclic voltammetry was performed to find the electrical potential corresponding to the greatest current response and the experiments were conducted using amperometric current-time mode (AMP*i-t). The physical limitations of SPEs as well as the detection limitations of the target, such as pH and temperature were tested. A concentration gradient of the target was fitted with a linear curve (R2 0.99), and a lower limit of detection of 14.5 μM. It was also found that both pH and temperature affect the current produced by acetaminophen at a fixed concentration, and that the sensors can detect target in a continuous flow. A flow apparatus consisting of an inlet and effluent pipe served as the flow model into which a rolled up flexible electrode array was inserted. The broader goal of this research is to develop a highly sensitive electrode array on flexible substrates which can detect multiple targets simultaneously. Acetaminophen was chosen due to its electro-active properties and its presence in most public water lines in the United States.
ContributorsMaxwell, Stephanie Ann (Author) / LaBelle, Jeffrey (Thesis director) / Allee, David (Committee member) / Barrett, The Honors College (Contributor) / Harrington Bioengineering Program (Contributor)
Created2014-05
Description
Consumption of seafood poses a substantial threat to global biodiversity. Chemical contamination found in both wild-caught and farmed seafood also presents significant health risks to consumers. Flame retardants, used in upholstery, plastics, clothing, and other products to reduce fire danger, are of particular concern as they are commonly found in the marine environment and permeate the tissues of fish that are sold for consumption via multiple pathways. By summarizing various metrics of sustainability and the mercury content in consumed species of fish and shellfish, researchers have found that high levels of chemical contamination was linked with lesser fishery sustainability. I conducted a literature review of flame retardant content in seafood to further compare contamination and sustainability in addition to the initial analysis with mercury. My review suggests that the widespread issue of fishery collapse could be alleviated by demonstrating to stakeholders that many unsustainable fish stocks are mutually disadvantageous for both human consumers and the environment. Future research should address the need for the collection of data that better represent actual global contaminant concentrations in seafood.
ContributorsNoziglia, Andrea Joyce (Author) / Gerber, Leah (Thesis director) / Smith, Andrew (Committee member) / Pratt, Stephen (Committee member) / Barrett, The Honors College (Contributor) / Herberger Institute for Design and the Arts (Contributor) / School of International Letters and Cultures (Contributor) / School of Life Sciences (Contributor)
Created2014-05
Description
In the face of the world's most pressing sustainability challenges, such as climate change, ecosystem degradation, and loss of biodiversity, the following questions must be explored: Why are these situation occurring? How can we understand their complexity? How can we research these challenges to mitigate negative outcomes? This thesis investigates the relationships between people and nature through coupled human and natural systems, or CHANS, and argues for a transdisciplinary research approach for sustainability science. The following questions and topics are discussed: 1. The Complexity of Sustainability and Implications for Traditional Research Approaches 2. Coupled Human and Natural Systems Research 3. What is Transdisciplinary Research, and How Does it Relate to the Living With Locusts Team's Coupled Human and Natural Systems Research? This thesis uses the case of a team researching international locust plagues to argue for this approach. The team's project is titled "Living With Locusts" and is directed by Arianne Cease of Arizona State University's School of Sustainability.
ContributorsLantz, Kayna Mishelle (Author) / Cease, Arianne (Thesis director) / Campbell, Jacob (Committee member) / School of Sustainability (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-12
Description
This paper analyzes the Flint, Michigan water crisis through research of water treatment in the United States. Pertinent scientific information is provided to serve as a background from which the reader can draw from to best understand the situation. The significance of water treatment in the context of sustainability is demonstrated through this descriptive case study of Flint. In ongoing efforts to supply safe drinking water to all communities, the comprehension of how the national framework works and why water is treated is paramount. Through the lens of society, this paper examines the science of water pollution, water treatment, treatment issues, and ensuing consequences. Water is a critical finite resource, and understanding how to most effectively use this limited resource is a major goal of the sustainable agenda.
ContributorsRahman, Natalia (Co-author) / Carrera, Steffani (Co-author) / Hartwell, Leland (Thesis director) / Hale, Annie (Committee member) / School of Sustainability (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05