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- All Subjects: Urban heat island
Description
This study was conducted as part of an underlying initiative to elucidate the mechanism of action of natural antibacterial clay minerals for application as therapeutic agents for difficult-to-treat infections such as methicillin-resistant Staphylococcus aureus (MRSA)-derived skin lesions and Buruli ulcer. The goal of this investigation was to determine whether exposure to the leachate of an antibacterial clay mineral, designated as CB, produced DNA double-strand breaks (DSBs) in Escherichia coli. A neutral comet assay for bacterial cells was adapted to assess DSB levels upon exposure to soluble antimicrobial compounds. Challenges involved with the adaptation process included comet visualization and data collection. To appropriately account for antimicrobial-mediated strand fragmentation, suitable control reactions comprised of exposures to water, ethanol, kanamycin, and bleomycin were developed and optimized for the assay. Bacterial exposure to CB resulted in significantly longer comet lengths compared to negative control exposures, suggesting that CB killing activity involves the induction of DNA DSBs. The results of this investigation further characterize the antimicrobial mechanisms associated with a particular clay mineral mixture. The adapted comet assay protocol described herein functions as an effective tool to assess double-strand fragmentation resulting from exposure to soluble antimicrobial compounds and to visually compare results from experimental and control reactions.
ContributorsSolanky, Dipesh (Author) / Haydel, Shelley (Thesis director) / Stout, Valerie (Committee member) / Adusumilli, Sarojini (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor)
Created2012-12
Description
This study seeks to determine the role of land architecture—the composition and configuration of land cover—as well as cadastral/demographic/economic factors on land surface temperature (LST) and the surface urban heat island effect of Phoenix, Arizona. It employs 1 m National Agricultural Imagery Program data of land-cover with 120mLandsat-derived land surface temperature, decomposed to 30 m, a new measure of configuration, the normalized moment of inertia, and U.S. Census data to address the question for two randomly selected samples comprising 523 and 545 residential neighborhoods (census blocks) in the city. The results indicate that, contrary to most other studies, land configuration has a stronger influence on LST than land composition. In addition, both land configuration and architecture combined with cadastral, demographic, and economic variables, capture a significant amount of explained variance in LST. The results indicate that attention to land architecture in the development of or reshaping of neighborhoods may ameliorate the summer extremes in LST.
ContributorsLi, Xiaoxiao (Author) / Li, Wenwen (Author) / Middel, Ariane (Author) / Harlan, Sharon L. (Author) / Brazel, Anthony J. (Author) / Turner II, B. L. (Author)
Created2015-12-29
Description
Urban growth, from regional sprawl to global urbanization, is the most rapid, drastic, and irreversible form of human modification to the natural environment. Extensive land cover modifications during urban growth have altered the local energy balance, causing the city warmer than its surrounding rural environment, a phenomenon known as an urban heat island (UHI). How are the seasonal and diurnal surface temperatures related to the land surface characteristics, and what land cover types and/or patterns are desirable for ameliorating climate in a fast growing desert city? This dissertation scrutinizes these questions and seeks to address them using a combination of satellite remote sensing, geographical information science, and spatial statistical modeling techniques.
This dissertation includes two main parts. The first part proposes to employ the continuous, pixel-based landscape gradient models in comparison to the discrete, patch-based mosaic models and evaluates model efficiency in two empirical contexts: urban landscape pattern mapping and land cover dynamics monitoring. The second part formalizes a novel statistical model called spatially filtered ridge regression (SFRR) that ensures accurate and stable statistical estimation despite the existence of multicollinearity and the inherent spatial effect.
Results highlight the strong potential of local indicators of spatial dependence in landscape pattern mapping across various geographical scales. This is based on evidence from a sequence of exploratory comparative analyses and a time series study of land cover dynamics over Phoenix, AZ. The newly proposed SFRR method is capable of producing reliable estimates when analyzing statistical relationships involving geographic data and highly correlated predictor variables. An empirical application of the SFRR over Phoenix suggests that urban cooling can be achieved not only by altering the land cover abundance, but also by optimizing the spatial arrangements of urban land cover features. Considering the limited water supply, rapid urban expansion, and the continuously warming climate, judicious design and planning of urban land cover features is of increasing importance for conserving resources and enhancing quality of life.
This dissertation includes two main parts. The first part proposes to employ the continuous, pixel-based landscape gradient models in comparison to the discrete, patch-based mosaic models and evaluates model efficiency in two empirical contexts: urban landscape pattern mapping and land cover dynamics monitoring. The second part formalizes a novel statistical model called spatially filtered ridge regression (SFRR) that ensures accurate and stable statistical estimation despite the existence of multicollinearity and the inherent spatial effect.
Results highlight the strong potential of local indicators of spatial dependence in landscape pattern mapping across various geographical scales. This is based on evidence from a sequence of exploratory comparative analyses and a time series study of land cover dynamics over Phoenix, AZ. The newly proposed SFRR method is capable of producing reliable estimates when analyzing statistical relationships involving geographic data and highly correlated predictor variables. An empirical application of the SFRR over Phoenix suggests that urban cooling can be achieved not only by altering the land cover abundance, but also by optimizing the spatial arrangements of urban land cover features. Considering the limited water supply, rapid urban expansion, and the continuously warming climate, judicious design and planning of urban land cover features is of increasing importance for conserving resources and enhancing quality of life.
ContributorsFan, Chao (Author) / Myint, Soe W (Thesis advisor) / Li, Wenwen (Committee member) / Rey, Sergio J (Committee member) / Arizona State University (Publisher)
Created2016
Description
Glioblastoma multiforme (GBMs) is the most prevalent brain tumor type and causes approximately 40% of all non-metastic primary tumors in adult patients [1]. GBMs are malignant, grade-4 brain tumors, the most aggressive classication as established by the World Health Organization and are marked by their low survival rate; the median survival time is only twelve months from initial diagnosis: Patients who live more than three years are considered long-term survivors [2]. GBMs are highly invasive and their diffusive growth pattern makes it impossible to remove the tumors by surgery alone [3]. The purpose of this paper is to use individual patient data to parameterize a model of GBMs that allows for data on tumor growth and development to be captured on a clinically relevant time scale. Such an endeavor is the rst step to a clinically applicable predictions of GBMs. Previous research has yielded models that adequately represent the development of GBMs, but they have not attempted to follow specic patient cases through the entire tumor process. Using the model utilized by Kostelich et al. [4], I will attempt to redress this deciency. In doing so, I will improve upon a family of models that can be used to approximate the time of development and/or structure evolution in GBMs. The eventual goal is to incorporate Magnetic Resonance Imaging (MRI) data into a parameterized model of GBMs in such a way that it can be used clinically to predict tumor growth and behavior. Furthermore, I hope to come to a denitive conclusion as to the accuracy of the Koteslich et al. model throughout the development of GBMs tumors.
ContributorsManning, Miles (Author) / Kostelich, Eric (Thesis director) / Kuang, Yang (Committee member) / Preul, Mark (Committee member) / Barrett, The Honors College (Contributor) / College of Liberal Arts and Sciences (Contributor)
Created2012-12