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- Creators: Turriziani Colonna, Federica
- Creators: Guo, Jia
Allan C. Wilson studied genes, proteins, and body structures of animals and humans in the US during the second half of the twentieth century. Wilson also studied human evolution. Although morphology and behaviors of humans (Homo sapiens) and great apes differ, Wilson found that they have biochemical and genetic similarities. Wilson and his colleagues calculated the time period of humans' and African apes' common ancestor. Wilson and his team also studied DNA outside of the nucleus in the cellular energy producing particles, called mitochondrial DNA (mtDNA), to study when different human groups evolved from each other.
Novel approaches for highly multiplexed single cell in situ transcriptomic analysis were developed by our group to enable single-cell comprehensive RNA profiling in their native spatial contexts. Reiterative FISH was demonstrated to be able to detect >100 RNA species in single cell in situ, while more sophisticated approaches, consecutive FISH (C-FISH) and switchable fluorescent oligonucleotide based FISH (SFO-FISH), have the potential for whole transcriptome profiling at the single molecule sensitivity. The introduction of a cleavable fluorescent tyramide even enables sensitive RNA profiling in intact tissues with high throughput. These approaches will have wide applications in studies of systems biology, molecular diagnosis and targeted therapies.
L'Institut Pasteur (The Pasteur Institute) is a non-profit private research institution founded by Louis Pasteur on 4 June 1887 in Paris, France. The Institute's research focuses on the study of infectious diseases, micro-organisms, viruses, and vaccines. As of 2014, ten scientists have received Nobel Prizes in physiology or medicine for the research they have done at the Pasteur Institute. Contrary to the way genetics was studied in US research universities during the mid-twentieth century, the genetic research conducted at the Pasteur Institute at the same time did not rest on a conceptual separation between embryology and evolution. According to historian Michel Morange from the Ecole Normale Superieure in Paris, France, this difference enabled Pasteurian scientists to develop the concepts of regulatory genes and of developmental genes.