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- Genre: Masters Thesis
- Genre: Interviews
- Genre: Interactive multimedia--Evaluation.
Description
Acute Myeloid Leukemia (AML) is a disease that occurs when genomic changes alter expression of key genes in myeloid blood cells. These changes cause them to resume an undifferentiated state, proliferate, and maintain growth throughout the body. AML is commonly treated with chemotherapy, but recent efforts to reduce therapy toxicity have focused on drugs that specifically target and inhibit protein products of the cancer’s aberrantly expressed genes. This method has proved difficult for some proteins because of structural challenges or mutations that confer resistance to therapy. One potential method of targeted therapy that circumvents these issues is the use of small molecules that stabilize DNA secondary structures called G-quadruplexes. G-quadruplexes are present in the promoter region of many potential oncogenes and have regulatory roles in their transcription. This study analyzes the therapeutic potential of the compound GQC-05 in AML. This compound was shown in vitro to bind and stabilize the regulatory G-quadruplex in the MYC oncogene, which is commonly misregulated in AML. Through qPCR and western blot analysis, a GQC-05 mediated downregulation of MYC mRNA and protein was observed in AML cell lines with high MYC expression. In addition, GQC-05 is able to reduce cell viability through induction of apoptosis in sensitive AML cell lines. Concurrent treatment of AML cell lines with GQC-05 and the MYC inhibitor (+)JQ1 showed an antagonistic effect, indicating potential competition in the silencing of MYC. However, GQC-05 is not able to reduce MYC expression significantly enough to induce apoptosis in less sensitive AML cell lines. This resistance may be due to the cells’ lack of dependence on other potential GQC-05 targets that may help upregulate MYC or stabilize its protein product. Three such genes identified by RNA-seq analysis of GQC-05 treated cells are NOTCH1, PIM1, and RHOU. These results indicate that the use of small molecules to target the MYC promoter G-quadruplex is a viable potential therapy for AML. They also support a novel mechanism for targeting other potentially key genetic drivers in AML and lay the groundwork for advances in treatment of other cancers driven by G-quadruplex regulated oncogenes.
ContributorsTurnidge, Megan (Author) / Lake, Douglas (Thesis advisor) / Kim, Suwon (Committee member) / Azorsa, David (Committee member) / Arizona State University (Publisher)
Created2017
Description
High-efficiency DC-DC converters make up one of the important blocks of state-of-the-art power supplies. The trend toward high level of transistor integration has caused load current demands to grow significantly. Supplying high output current and minimizing output current ripple has been a driving force behind the evolution of Multi-phase topologies. Ability to supply large output current with improved efficiency, reduction in the size of filter components, improved transient response make multi-phase topologies a preferred choice for low voltage-high current applications.
Current sensing capability inside a system is much sought after for applications which include Peak-current mode control, Current limiting, Overload protection. Current sensing is extremely important for current sharing in Multi-phase topologies. Existing approaches such as Series resistor, SenseFET, inductor DCR based current sensing are simple but their drawbacks such low efficiency, low accuracy, limited bandwidth demand a novel current sensing scheme.
This research presents a systematic design procedure of a 5V - 1.8V, 8A 4-Phase Buck regulator with a novel current sensing scheme based on replication of the inductor current. The proposed solution consists of detailed system modeling in PLECS which includes modification of the peak current mode model to accommodate the new current sensing element, derivation of power-stage and Plant transfer functions, Controller design. The proposed model has been verified through PLECS simulations and compared with a transistor-level implementation of the system. The time-domain parameters such as overshoot and settling-time simulated through transistor-level
implementation is in close agreement with the results obtained from the PLECS model.
Current sensing capability inside a system is much sought after for applications which include Peak-current mode control, Current limiting, Overload protection. Current sensing is extremely important for current sharing in Multi-phase topologies. Existing approaches such as Series resistor, SenseFET, inductor DCR based current sensing are simple but their drawbacks such low efficiency, low accuracy, limited bandwidth demand a novel current sensing scheme.
This research presents a systematic design procedure of a 5V - 1.8V, 8A 4-Phase Buck regulator with a novel current sensing scheme based on replication of the inductor current. The proposed solution consists of detailed system modeling in PLECS which includes modification of the peak current mode model to accommodate the new current sensing element, derivation of power-stage and Plant transfer functions, Controller design. The proposed model has been verified through PLECS simulations and compared with a transistor-level implementation of the system. The time-domain parameters such as overshoot and settling-time simulated through transistor-level
implementation is in close agreement with the results obtained from the PLECS model.
ContributorsBurli, Venkatesh (Author) / Bakkaloglu, Bertan (Thesis advisor) / Garrity, Douglas (Committee member) / Kitchen, Jennifer (Committee member) / Arizona State University (Publisher)
Created2017
Description
The media disperses a large amount of information daily pertaining to political events social movements, and societal conflicts. Media pertaining to these topics, no matter the format of publication used, are framed a particular way. Framing is used not for just guiding audiences to desired beliefs, but also to fuel societal change or legitimize/delegitimize social movements. For this reason, tools that can help to clarify when changes in social discourse occur and identify their causes are of great use. This thesis presents a visual analytics framework that allows for the exploration and visualization of changes that occur in social climate with respect to space and time. Focusing on the links between data from the Armed Conflict Location and Event Data Project (ACLED) and a streaming RSS news data set, users can be cued into interesting events enabling them to form and explore hypothesis. This visual analytics framework also focuses on improving intervention detection, allowing users to hypothesize about correlations between events and happiness levels, and supports collaborative analysis.
ContributorsSteptoe, Michael (Author) / Maciejewski, Ross (Thesis advisor) / Davulcu, Hasan (Committee member) / Corman, Steven (Committee member) / Arizona State University (Publisher)
Created2017
Description
The interaction between visual fixations during planning and performance in a
dexterous task was analyzed. An eye-tracking device was affixed to subjects during
sequences of null (salient center of mass) and weighted (non salient center of mass) trials
with unconstrained precision grasp. Subjects experienced both expected and unexpected
perturbations, with the task of minimizing object roll. Unexpected perturbations were
controlled by switching weights between trials, expected perturbations were controlled by
asking subjects to rotate the object themselves. In all cases subjects were able to
minimize the roll of the object within three trials. Eye fixations were correlated with
object weight for the initial context and for known shifts in center of mass. In subsequent
trials with unexpected weight shifts, subjects appeared to scan areas of interest from both
contexts even after learning present orientation.
dexterous task was analyzed. An eye-tracking device was affixed to subjects during
sequences of null (salient center of mass) and weighted (non salient center of mass) trials
with unconstrained precision grasp. Subjects experienced both expected and unexpected
perturbations, with the task of minimizing object roll. Unexpected perturbations were
controlled by switching weights between trials, expected perturbations were controlled by
asking subjects to rotate the object themselves. In all cases subjects were able to
minimize the roll of the object within three trials. Eye fixations were correlated with
object weight for the initial context and for known shifts in center of mass. In subsequent
trials with unexpected weight shifts, subjects appeared to scan areas of interest from both
contexts even after learning present orientation.
ContributorsSmith, Michael David (Author) / Santello, Marco (Thesis advisor) / Buneo, Christopher (Committee member) / Schaefer, Sydney (Committee member) / Arizona State University (Publisher)
Created2017
Description
Activated Carbon has been used for decades to remove organics from water at large scale in municipal water treatment as well as at small scale in Point of Use (POU) and Point of Entry (POE) water treatment. This study focused on Granular Activated Carbon (GAC) and also activated Carbon Block (CB) were studied.
This thesis has three related elements for organics control in drinking water. First, coagulation chemistry for Alum and Aluminum Chlorohydrate (ACH) was optimized for significant organics removal to address membrane fouling issue at a local municipal water treatment plant in Arizona. Second, Rapid Small Scale Column Tests were conducted for removal of Perfluorinated compounds (PFC), PFC were present in groundwater at a local site in Arizona at trace levels with combined concentration of Perfluorooctaneoic Acid (PFOA) and Perfloorooctanesulfonic Acid (PFOS) up to 245 ng/L. Groundwater from the concerned site is used as drinking water source by a private utility. PFC Removal was evaluated for different GAC, influent concentrations and particle sizes. Third, a new testing protocol (Mini Carbon Block (MCB)) for bench scale study of POU water treatment device, specifically carbon block filter was developed and evaluated. The new bench scale decreased the hydraulic requirements by 60 times approximately, which increases the feasibility to test POU at a lab scale. It was evaluated for a common POU organic contaminant: Chloroform, and other model contaminants.
10 mg/L of ACH and 30 mg/L of Alum with pH adjustment were determined as optimal coagulant doses. Bituminous coal based GAC was almost three times better than coconut shell based GAC for removing PFC. Multiple tests with MCB suggested no short circuiting and consistent performance for methylene blue though chloroform removal tests underestimated full scale carbon block performance but all these tests creates a good theoretical and practical fundament for this new approach and provides directions for future researchers.
This thesis has three related elements for organics control in drinking water. First, coagulation chemistry for Alum and Aluminum Chlorohydrate (ACH) was optimized for significant organics removal to address membrane fouling issue at a local municipal water treatment plant in Arizona. Second, Rapid Small Scale Column Tests were conducted for removal of Perfluorinated compounds (PFC), PFC were present in groundwater at a local site in Arizona at trace levels with combined concentration of Perfluorooctaneoic Acid (PFOA) and Perfloorooctanesulfonic Acid (PFOS) up to 245 ng/L. Groundwater from the concerned site is used as drinking water source by a private utility. PFC Removal was evaluated for different GAC, influent concentrations and particle sizes. Third, a new testing protocol (Mini Carbon Block (MCB)) for bench scale study of POU water treatment device, specifically carbon block filter was developed and evaluated. The new bench scale decreased the hydraulic requirements by 60 times approximately, which increases the feasibility to test POU at a lab scale. It was evaluated for a common POU organic contaminant: Chloroform, and other model contaminants.
10 mg/L of ACH and 30 mg/L of Alum with pH adjustment were determined as optimal coagulant doses. Bituminous coal based GAC was almost three times better than coconut shell based GAC for removing PFC. Multiple tests with MCB suggested no short circuiting and consistent performance for methylene blue though chloroform removal tests underestimated full scale carbon block performance but all these tests creates a good theoretical and practical fundament for this new approach and provides directions for future researchers.
ContributorsAshani, Harsh Satishbhai (Author) / Westerhoff, Paul (Thesis advisor) / Hristovski, Kiril (Committee member) / Conroy-Ben, Otakuye (Committee member) / Arizona State University (Publisher)
Created2017
Description
This study utilizes semiotic phenomenology as a method of inquiry to describe the lived experiences of Lesbian, Gay, Bisexual, Transgender, Queer (LGBTQ) gamers (gaymers). I begin by discussing my issues with the current gaming literature, arguing that the gamer community is a space that privileges cis, heterosexual, and hypermasculine men while oppressing those who may not fit this mold. I discuss the shortcomings of the current literature that attempts to critically look at race and gaming, noting that race in the gaming community is still portrayed as secondary. I focus special attention to how this space allows for more inclusion than the larger gamer and LGBTQ communities while also critiquing those whom this space privileges. Through interviews of members of the local gaymer organization, the Phoenix Gaymers, I discuss ways in which the gaymer community is more inclusive and conscious of others but still follows forms of what I describe to be gaymer privilege. I focus on gaymer privilege within the intersections of race, gender, and sexuality, where I argue from the phenomenological descriptions, reductions, and interpretations that there are still overt issues of sexism and transphobia as well as implicit issues of white privilege. While I describe the issues that are found within the Phoenix Gaymers, I also attempt to provide suggestions for change within the organization as well as in academic scholarship to create more awareness and inclusion for female, transgender, genderqueer, and queer people of color gaymers.
ContributorsOmori, Jeremy Michael (Author) / Sandlin, Jennifer (Thesis advisor) / Martinez, Jacqueline M (Committee member) / Pérez, Kimberlee (Committee member) / Linde, Jennifer (Committee member) / Arizona State University (Publisher)
Created2017
Description
A medical control system, a real-time controller, uses a predictive model of human physiology for estimation and controlling of drug concentration in the human body. Artificial Pancreas (AP) is an example of the control system which regulates blood glucose in T1D patients. The predictive model in the control system such as Bergman Minimal Model (BMM) is based on physiological modeling technique which separates the body into the number of anatomical compartments and each compartment's effect on body system is determined by their physiological parameters. These models are less accurate due to unaccounted physiological factors effecting target values. Estimation of a large number of physiological parameters through optimization algorithm is computationally expensive and stuck in local minima. This work evaluates a machine learning(ML) framework which has an ML model guided through physiological models. A support vector regression model guided through modified BMM is implemented for estimation of blood glucose levels. Physical activity and Endogenous glucose production are key factors that contribute in the increased hypoglycemia events thus, this work modifies Bergman Minimal Model ( Bergman et al. 1981) for more accurate estimation of blood glucose levels. Results show that the SVR outperformed BMM by 0.164 average RMSE for 7 different patients in the free-living scenario. This computationally inexpensive data driven model can potentially learn parameters more accurately with time. In conclusion, advised prediction model is promising in modeling the physiology elements in living systems.
ContributorsAgrawal, Anurag (Author) / Gupta, Sandeep K. S. (Thesis advisor) / Banerjee, Ayan (Committee member) / Kudva, Yogish (Committee member) / Arizona State University (Publisher)
Created2017
Description
This phenomenological study explores the question: What are the lived experiences of Arizonans who identify their gender identities as ‘non-binary’? (‘non-binary’ defined here as anyone who identifies their gender as something other than ‘always and exclusively male or always and exclusively female’). The study explores the lived realities of four non-binary identified transgender people living in Arizona. Each participant took a short survey and conducted a 45-minute in-person interview, conducted through phenomenological questioning to evoke deep descriptions of experience. After analyzing the results through feminist hermeneutic phenomenology, this study suggests that the experience of non-binary gender identity presents an essential pattern of cultivating self-realization. The essential themes of internal recognition, external presentation, and movement toward wellness fell into this pattern. The United States has conceptualized transgender identity in many ways, from pathologizing to politicizing, to medically affirming views. Although the literature on this topic is quite small, there is no doubt that non-binary transgender people exist in U.S. public life. Ultimately, if non-binary people are to find affirming paths toward self-realization, they must be heard from their own experiences in their own voices.
ContributorsSkinner, Ashton (Author) / Sandlin, Jennifer (Thesis advisor) / Nakagawa, Kathryn (Committee member) / Santos, Carlos (Committee member) / Arizona State University (Publisher)
Created2017
Description
This article proposes a new information-based subdata selection (IBOSS) algorithm, Squared Scaled Distance Algorithm (SSDA). It is based on the invariance of the determinant of the information matrix under orthogonal transformations, especially rotations. Extensive simulation results show that the new IBOSS algorithm retains nice asymptotic properties of IBOSS and gives a larger determinant of the subdata information matrix. It has the same order of time complexity as the D-optimal IBOSS algorithm. However, it exploits the advantages of vectorized calculation avoiding for loops and is approximately 6 times as fast as the D-optimal IBOSS algorithm in R. The robustness of SSDA is studied from three aspects: nonorthogonality, including interaction terms and variable misspecification. A new accurate variable selection algorithm is proposed to help the implementation of IBOSS algorithms when a large number of variables are present with sparse important variables among them. Aggregating random subsample results, this variable selection algorithm is much more accurate than the LASSO method using full data. Since the time complexity is associated with the number of variables only, it is also very computationally efficient if the number of variables is fixed as n increases and not massively large. More importantly, using subsamples it solves the problem that full data cannot be stored in the memory when a data set is too large.
ContributorsZheng, Yi (Author) / Stufken, John (Thesis advisor) / Reiser, Mark R. (Committee member) / McCulloch, Robert (Committee member) / Arizona State University (Publisher)
Created2017
Description
Advancements in thermal interface materials (TIMs) allows for the creation of new and more powerful electronics as they increase the heat transfer from the component to the heat sink. Current industrial options provide decent heat transfer, but the creation of TIMs with higher thermal conductivities is needed. In addition, if these TIMs are elastic in nature, their effectiveness can greatly increase as they can deal with changing interfaces without degradation of their properties. The research performed delves into this idea, creating elastic TIMs using liquid metal (LM), in this case galinstan, along with other matrix particles embedded in Polydimethylsiloxane (PDMS) to create an easy to use, relatively inexpensive, thermally conductive, but electrically insulative, pad with increased thermal conductivity from industrial solutions.
The pads were created using varying amounts of LM and matrix materials ranging from copper microspheres to diamond powder mixed into PDMS using a high-speed mixer. The material was then cast into molds and cured to create the pads. Once the pads were created, the difficulty came in quantifying their thermal properties. A stepped bar apparatus (SBA) following ASTM D5470 was created to measure the thermal resistance of the pads but it was determined that thermal conductivity was a more usable metric of the pads’ performance. This meant that the pad’s in-situ thickness was needed during testing, prompting the installation of a linear encoder to measure the thickness. The design and analysis of the necessary modification and proposed future design is further detailed in the following paper.
The pads were created using varying amounts of LM and matrix materials ranging from copper microspheres to diamond powder mixed into PDMS using a high-speed mixer. The material was then cast into molds and cured to create the pads. Once the pads were created, the difficulty came in quantifying their thermal properties. A stepped bar apparatus (SBA) following ASTM D5470 was created to measure the thermal resistance of the pads but it was determined that thermal conductivity was a more usable metric of the pads’ performance. This meant that the pad’s in-situ thickness was needed during testing, prompting the installation of a linear encoder to measure the thickness. The design and analysis of the necessary modification and proposed future design is further detailed in the following paper.
ContributorsKemme, Nicholas (Author) / Rykaczewski, Konrad (Thesis advisor) / Wang, Robert (Thesis advisor) / Wang, Liping (Committee member) / Arizona State University (Publisher)
Created2017